Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,503,270	T→C	T406A (ACC→GCC)	fryA ←	putative PTS multiphosphoryl transfer protein FryA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,503,270	0	T	C	100.0%	193.6 / NA	55	T406A (ACC→GCC)	fryA	putative PTS multiphosphoryl transfer protein FryA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/27);  total (28/27)

TGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTGTCGCCCGCCGCCAGTAGCACCTGCTGGTAGGCTTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTCGGTACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCGGTGCCGATATTCGCCGCAATATCAATACGCTTGTTATCACGGGAATAGGCCAGCTGCGCGGCAGCCTGCGCCTGTT  >  NC_000913/2503135‑2503404                                                                                                                                        |                                                                                                                                       tGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTGTCGCCCGCCGCCAGTAGCACCTGCTGGTAGGCTTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTCGGCACgg                                                                                                                                    >  2:702768/1‑140 (MQ=255)   tttttATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTGTCGCCCGCCGCCAGTAGCACCTGCTGGTAGGCTTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTTGGCACGGaa                                                                                                                                  <  2:274738/139‑1 (MQ=255)   cTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTGTCGCCCGCCGCCAGTAGCACCTGCTGGTAGGCTTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTCGGCACGGaa                                                                                                                                  <  2:528168/140‑1 (MQ=255)   cTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTGTCGCCCGCCGCCAGTAGCACCTGCTGGTAGGCTTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTCGGCACGGaa                                                                                                                                  >  1:541115/1‑140 (MQ=255)   cTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTGTCGCCCGCCGCCAGTAGCACCTGCTGGTAGGCTTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTCGGCACGGaa                                                                                                                                  <  2:541115/140‑1 (MQ=255)   cTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTGTCGCCCGCCGCCAGTAGCACCTGCTGGTAGGCTTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTCGGCACGGaa                                                                                                                                  <  2:1247597/140‑1 (MQ=255)   cTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTGTCGCCCGCCGCCAGTAGCACCTGCTGGTAGGCTTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTCGGCACGGaa                                                                                                                                  <  2:92721/140‑1 (MQ=255)        aTCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTGTCGCCCGCCGCCAGTAGCACCTGCTGGTAGGCTTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTCGGCACGGaa                                                                                                                                  >  2:601873/1‑135 (MQ=255)        aTCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTGTCGCCCGCCGCCAGTAGCACCTGCTGGTAGGCTTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTCGGCACGGaa                                                                                                                                  <  1:601873/135‑1 (MQ=255)                          cGTGCGGAAGATAATCGGCTTGTCGCCCGCCGCCAGTAGCACCTGCTGGTAGGCTTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGt                                                                                                           >  1:127507/1‑140 (MQ=255)                                          ggCTTGTCGCCCGCCGCCAGTAGCACCTGCTGGTAGGCTTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTgg                                                                                           <  2:1086614/140‑1 (MQ=255)                                          ggCTTGTCGCCCGCCGCCAGTAGCACCTGCTGGTAGGCTTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTgg                                                                                           <  2:301036/140‑1 (MQ=255)                                           gCTTGTCGCCCGCCGCCAGTAGCACCTGCTGGTAGGCTTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGgc                                                                                          >  2:501/1‑140 (MQ=255)                                             ttGTCGCCCGCCGCCAGTAGCACCTGCTGGTAGGCTTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGgcgc                                                                                        >  2:72690/1‑140 (MQ=255)                                               gTCGCCCGCCGCCAGTAGCACCTGCTGGTAGGCTTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTgg                                                                                           <  1:498318/135‑1 (MQ=255)                                               gTCGCCCGCCGCCAGTAGCACCTGCTGGTAGGCTTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTgg                                                                                           >  2:498318/1‑135 (MQ=255)                                                  gcccgccGCCAGTAGCACCTGCTGGTAGGCTTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCa                                                                                   <  2:634201/140‑1 (MQ=255)                                                      gccgccAGTAGCACCTGCTGGTAGGCTTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGc                                                                               <  1:469028/140‑1 (MQ=255)                                                       ccgccAGTAGCACCTGCTGGTAGGCTTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCg                                                                              <  1:702768/140‑1 (MQ=255)                                                           cAGTAGCACCTGCTGGTAGGCTTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCGGTGc                                                                          <  1:881169/140‑1 (MQ=255)                                                                  cccTGCTGGTAGGCTTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCGGTGCCGATAtt                                                                   <  2:208429/139‑1 (MQ=255)                                                                      gctgGTAGGCTTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCGGTGCCGATATTcgcc                                                               >  1:510529/1‑140 (MQ=255)                                                                      gctgGTAGGCTTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCGGTGCCGATATTcgcc                                                               >  2:616208/1‑140 (MQ=255)                                                                        tgGTAGGCTTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCGGTGCCGATATTcgccgc                                                             >  2:98431/1‑140 (MQ=255)                                                                        tgGTAGGCTTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCGGTGCCGATATTcgccgc                                                             >  2:252184/1‑140 (MQ=255)                                                                        tgGTAGGCTTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTCGACACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCg                                                                              <  2:1029872/123‑1 (MQ=255)                                                                        tgGTAGGCTTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTCGACACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCg                                                                              >  1:1029872/1‑123 (MQ=255)                                                                          gTAGGCTTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCGGTGCCGATATTCGCCGCaa                                                           <  2:207268/140‑1 (MQ=255)                                                                           tAGGCTTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGACTGGCGCTTCCAGAGCGGTGCCGATATTcgccg                                                              <  2:422917/136‑1 (MQ=255)                                                                           tAGGCTTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGACTGGCGCTTCCAGAGCGGTGCCGATATTcgccg                                                              >  1:422917/1‑136 (MQ=255)                                                                               cTTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTCGGCACGGAACAGCCCGACACCTTGCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCGGTGCCGATATTCGCCGCAATATCa                                                      >  2:971658/1‑140 (MQ=255)                                                                               cTTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCGGTGCCGATATTCGCCGCAATATCa                                                      >  2:153755/1‑140 (MQ=255)                                                                               cTTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCGGTGCCGATATTCGCCGCAATATCa                                                      >  2:155637/1‑140 (MQ=255)                                                                                  cAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCGGTGCCGATATTCGCCGCAATATCAATa                                                   <  1:448522/140‑1 (MQ=255)                                                                                    aaTTGCTCCTGCTCGTCCGGCGCGCTGTCG‑GATCCATATAGAGCATTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCGGTGCCGATATTCGCCGCAATATCAATa                                                   >  2:190811/1‑137 (MQ=255)                                                                                    aaTTGCTCCTGCTCGTCCGGCGCGCTGTCG‑GATCCATATAGAGCATTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCGGTGCCGATATTCGCCGCAATATCAATa                                                   <  1:190811/137‑1 (MQ=255)                                                                                              gCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCGGTGCCGATATTCGCCGCAATATCAATACGCTTGTTATCa                                       <  1:501/140‑1 (MQ=255)                                                                                                              gTCGCGATCCATATAGAGCATTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCGGTGCCGATATTCGCCGCAATATCAATACGCTTGTTATCACGGGAATAGGCCAGCt                       <  2:510529/140‑1 (MQ=255)                                                                                                                    aTCCATATAGAGCATTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCGGTGCCGATATTCGCCGCAATATCAATACGCTTGGTATCACGGGAATAGGCAAGCTGCGCgg                 <  1:616208/140‑1 (MQ=255)                                                                                                                         tataGAGCATTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCGGTGCCGATATTCGCCGCAATATCAATACGCTTGTTATCACGGGAATAGGCCAGCTGCGCGGCAGcc            <  1:72690/140‑1 (MQ=255)                                                                                                                         tataGAGCATTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCGGTGCCGATATTCGCCGCAATATCAATACGCTTGTTATCACGGGAATAGGCCAGCTGCGCGGCAGcc            <  2:722462/140‑1 (MQ=255)                                                                                                                              agCATTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCGGTGCCGATATTCGCCGCAATATCAATACGCTTGTTATCACGGGAATAGGCCAGCTGCGCgg                 <  1:1117185/130‑1 (MQ=255)                                                                                                                              agCATTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCGGTGCCGATATTCGCCGCAATATCAATACGCTTGTTATCACGGGAATAGGCCAGCTGCGCgg                 >  2:1117185/1‑130 (MQ=255)                                                                                                                               gCATTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCGGTGCCGATATTCGCCGCAATATCAATACGCTTGTTATCACGGGAATTGGCCAGCTGCGCGGCAGCCTGCGcc      >  1:25994/1‑140 (MQ=255)                                                                                                                               gCATTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCGGTGCCGATATTCGCCGCAATATCAATACGCTTGTTATCACGGGAATAGGCCAGCTGCGCGGCAGCCTGCGcc      >  2:945538/1‑140 (MQ=255)                                                                                                                                cATTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCGGTGCCGATATTCGCCGCAATATCAATACGCTTGTTATCACGGGAATAGGCCAGCTGCGCGGCAGCCTgcgc       >  1:513108/1‑138 (MQ=255)                                                                                                                                cATTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCGGTGCCGATATTCGCCGCAATATCAATACGCTTGTTATCACGGGAATAGGCCAGCTGCGCGGCAGCCTgcgc       <  2:513108/138‑1 (MQ=255)                                                                                                                                cATTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCGGTGCCGATATTCGCCGCAATATCAATACGCTTGTTATCACGGGAATAGGCCAGCTGCGCGGCAGCCTGCGCCt     >  2:252009/1‑140 (MQ=255)                                                                                                                                 aTTTCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCGGTGCCGATATTCGCCGCAATATCAATACGCTTGTTATCACGGGAATAGGCCAGCTGCGCGGCAGCCTGCGCCtg    >  2:148437/1‑140 (MQ=255)                                                                                                                                  tttCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCGGTGCCGATATTCGCCGCAATATCAATACGCTTGTTATCACGGGAATAGGCCAGCTGCGCGGCAGcc            <  2:1066843/131‑1 (MQ=255)                                                                                                                                  tttCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCGGTGCCGATATTCGCCGCAATATCAATACGCTTGTTATCACGGGAATAGGCCAGCTGCGCGGCAGcc            >  1:1066843/1‑131 (MQ=255)                                                                                                                                   ttCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCGGTGCCGATATTCGCCGCAATATCAATACGCTTGTTATCACGGGAATAGGCCAGCTGCGCGGCAGCCTGCGCCtgtt  >  1:413742/1‑140 (MQ=255)                                                                                                                                   ttCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCGGTGCCGATATTCGCCGCAATATCAATACGCTTGTTATCACGGGAATAGGCCAGCTGCGCGGCAGCCTGCGCCtgtt  >  2:680688/1‑140 (MQ=255)                                                                                                                                   ttCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCGGTGCCGATATTCGCCGCAATATCAATACGCTTGTTATCACGGGAATAGGCCAGCTGCGCGGCAGCCTGCGCCtgtt  >  1:352973/1‑140 (MQ=255)                                                                                                                                   ttCGGCACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCGGTGCCGATATTCGCCGCAATATCAATACGCTTGTTATCACGGGAATAGGCCAGCTGCGCGGCAGCCTGCGCCtgtt  >  2:306393/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       TGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTGTCGCCCGCCGCCAGTAGCACCTGCTGGTAGGCTTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCATATAGAGCATTTCGGTACGGAACAGCCCGACACCTTCCGCGCCGTTGGCAAACGCGCCTGGCGCTTCCAGAGCGGTGCCGATATTCGCCGCAATATCAATACGCTTGTTATCACGGGAATAGGCCAGCTGCGCGGCAGCCTGCGCCTGTT  >  NC_000913/2503135‑2503404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑