Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,527,823	T→C	V293A (GTG→GCG)	yfeH →	putative solute:Na(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,527,823	0	T	C	100.0%	158.8 / NA	46	V293A (GTG→GCG)	yfeH	putative solute:Na(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/21);  total (25/21)

CGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCA  >  NC_000913/2527686‑2527957                                                                                                                                          |                                                                                                                                       cGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGgcgc                                                                                                                                      >  2:409479/1‑140 (MQ=255)   tCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGcgctg                                                                                                                                    <  1:816451/140‑1 (MQ=255)    cTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGcgctgc                                                                                                                                   >  2:385234/1‑140 (MQ=255)            cACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCtgatg                                                                                                                           >  2:1252710/1‑140 (MQ=255)              cGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCCGTGATCGGTATGATGGCGCTGCCCCTgatgat                                                                                                                         >  2:1111958/1‑140 (MQ=255)                   aCTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTcc                                                                                                                    >  1:1041100/1‑140 (MQ=255)                   aCTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTcc                                                                                                                    <  1:965874/140‑1 (MQ=255)                    cTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCa                                                                                                                   <  2:874914/140‑1 (MQ=255)                           tCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATc                                                                                                            >  1:270297/1‑140 (MQ=255)                                 aGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTg                                                                                                      <  1:1252710/140‑1 (MQ=255)                                 aGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTg                                                                                                      >  1:392532/1‑140 (MQ=255)                                      gATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGt                                                                                                 <  1:852304/140‑1 (MQ=255)                                      gATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATCCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGt                                                                                                 <  1:18657/140‑1 (MQ=255)                                       aTGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTc                                                                                                >  2:151539/1‑140 (MQ=255)                                       aTGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTc                                                                                                >  2:40936/1‑140 (MQ=255)                                       aTGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTc                                                                                                >  2:108651/1‑140 (MQ=255)                                       aTGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGAAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATcc                                                                                                           >  1:95075/1‑129 (MQ=255)                                       aTGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGAAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATcc                                                                                                           <  2:95075/129‑1 (MQ=255)                                           aaTTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCtgtg                                                                                            <  1:442057/140‑1 (MQ=255)                                           aaTTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCtgtg                                                                                            <  1:1111958/140‑1 (MQ=255)                                                 tATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACGTCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGc                                                                                      >  2:365836/1‑140 (MQ=255)                                                     gTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGgc                                                                                  >  2:818203/1‑140 (MQ=255)                                                     gTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGgc                                                                                  >  1:1177142/1‑140 (MQ=255)                                                        ttttGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGAT‑GCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGt                                                                              >  1:383038/1‑140 (MQ=255)                                                         tttGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGt                                                                              <  2:270297/140‑1 (MQ=255)                                                               gTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATAc                                                                        >  2:841897/1‑140 (MQ=255)                                                                  cGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACaaa                                                                     >  2:413172/1‑140 (MQ=255)                                                                            tCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCg                                                           >  1:830185/1‑140 (MQ=255)                                                                                gCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACa                                                       <  2:1041100/140‑1 (MQ=255)                                                                                        cATCCCGATGGCAAACATTCTGTTCCCCACGTCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAgg                                               <  1:365836/140‑1 (MQ=255)                                                                                        cATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAgg                                               <  1:841897/140‑1 (MQ=255)                                                                                          tCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGgcg                                             >  1:1174038/1‑140 (MQ=255)                                                                                           cccGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGgcgc                                            <  1:108651/140‑1 (MQ=255)                                                                                                      aCATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAagc                                 >  2:679471/1‑140 (MQ=255)                                                                                                        aTTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAagcag                               <  2:1174038/140‑1 (MQ=255)                                                                                                             gTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCg                          >  2:126836/1‑140 (MQ=255)                                                                                                              ttCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGa                         >  1:16353/1‑140 (MQ=255)                                                                                                                  ccACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATaaa                     >  2:211834/1‑140 (MQ=255)                                                                                                                      aTCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCtt                 <  1:409479/140‑1 (MQ=255)                                                                                                                        cGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATa                       <  1:682986/132‑1 (MQ=255)                                                                                                                        cGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATa                       >  2:682986/1‑132 (MQ=255)                                                                                                                        cGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTaa               >  2:842876/1‑140 (MQ=255)                                                                                                                             aTCGGTATGATGGCGTTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCgg          >  2:1147745/1‑140 (MQ=255)                                                                                                                             aTCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCgg          >  2:159264/1‑140 (MQ=255)                                                                                                                              tCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGa         <  2:383038/140‑1 (MQ=255)                                                                                                                              tCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGa         <  1:211834/140‑1 (MQ=255)                                                                                                                              tCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGa         <  1:40936/140‑1 (MQ=255)                                                                                                                              tCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGa         <  2:1078481/140‑1 (MQ=255)                                                                                                                               cGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGAc        <  2:16353/140‑1 (MQ=255)                                                                                                                                     gatgGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCa  <  2:756199/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       CGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCA  >  NC_000913/2527686‑2527957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑