Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,565,722	A→G	V388A (GTC→GCC)	eutA ←	reactivating factor for ethanolamine ammonia‑lyase EutBC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,565,722	0	A	G	100.0%	167.2 / NA	48	V388A (GTC→GCC)	eutA	reactivating factor for ethanolamine ammonia‑lyase EutBC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/22);  total (26/22)

ACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCAAAGTCCTGCCCGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAA  >  NC_000913/2565597‑2565851                                                                                                                              |                                                                                                                                  aCGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCAAAGTCCTGCCCGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACcgcg                                                                                                                     <  2:427416/140‑1 (MQ=255)     gAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCAAAGTCCTGCCCGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGt                                                                                                                 <  1:794510/140‑1 (MQ=255)      aGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCAAAGTCCTGCCCGGCCACCACCAGCAGAGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTa                                                                                                                >  2:875194/1‑140 (MQ=255)             gtAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCAAAGTCCTGCCCGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACTCGTTGATGGCCGTCAGTACCGCGGCGTAACGcaca                                                                                                         >  2:866653/1‑140 (MQ=255)                 cTGTGGGCGCAACAACATGCCCAGAGCTTTACCAAAGTCCTGCCCGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCa                                                                                                     <  1:362228/140‑1 (MQ=255)                   gtgGGCGCAACAACATGCCCAGAGCTTTACCAAAGTCCTGCCCGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGc                                                                                                   >  1:923483/1‑140 (MQ=255)                      ggCGCAACAACATGCCCAGAGCTTTACCAAAGTCCTGCCCGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAg                                                                                                >  2:796223/1‑140 (MQ=255)                      ggCGCAACAACATGCCCAGAGCTTTACCAAAGTCCTGCCCGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAg                                                                                                >  1:556305/1‑140 (MQ=255)                                   gCCCAGAGCTTTACCAAAGTCCTGCCCGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCa                                                                                   <  2:276081/140‑1 (MQ=255)                                       agagCTTTACCAAAGTCCTGCCCGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCAc                                                                               >  2:635237/1‑140 (MQ=255)                                       agagCTTTACCAAAGTCCTGCCCGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCAc                                                                               >  1:466241/1‑140 (MQ=255)                                        gagCTTTACCAAAGTCCTGCCCGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACg                                                                              >  1:666767/1‑140 (MQ=255)                                         agCTTTACCAAAGTCCTGCCCGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGt                                                                             >  2:1010291/1‑140 (MQ=255)                                             ttACCAAAGTCCTGCCCGGCCACCACCAGCAGGGGATGCGGATCCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTAcgc                                                                         >  2:612075/1‑140 (MQ=255)                                              tACCAAAGTCCTGCCCGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTAcgcg                                                                        >  1:1239952/1‑140 (MQ=255)                                              tACCAAAGTCCTGCCCGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTAcgcg                                                                        >  2:907525/1‑140 (MQ=255)                                               aCCAAAGTCCTGCCCGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGt                                                                       <  2:556305/140‑1 (MQ=255)                                               aCCAAAGTCCTGCCCGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGt                                                                       >  1:797918/1‑140 (MQ=255)                                                    aGTCCTGCCCGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGtt                                                                  >  1:952324/1‑140 (MQ=255)                                                      tCCTGCCCGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGtttt                                                                <  2:797918/140‑1 (MQ=255)                                                      tCCTGCCCGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGttt                                                                 <  1:545006/139‑1 (MQ=255)                                                      tCCTGCCCGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGttt                                                                 >  2:545006/1‑139 (MQ=255)                                                       ccTGCCCGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTg                                                               <  1:1010291/140‑1 (MQ=255)                                                        cTGCCCGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTgg                                                              >  2:899528/1‑140 (MQ=255)                                                        cTGCCCGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTgg                                                              >  1:845245/1‑140 (MQ=255)                                                             cGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCa                                                         <  1:877752/140‑1 (MQ=255)                                                                ccaccaccaGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGa                                                      >  1:1050348/1‑140 (MQ=255)                                                                 caccaccaGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGAt                                                     <  2:64971/140‑1 (MQ=255)                                                                      ccaGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGc                                                <  1:796223/140‑1 (MQ=255)                                                                          cagGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGaa                                            <  2:1050348/140‑1 (MQ=255)                                                                             gggATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCa                                         >  2:698439/1‑140 (MQ=255)                                                                              ggATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAg                                        >  1:135697/1‑140 (MQ=255)                                                                                 tGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAgcgc                                     >  2:1117739/1‑140 (MQ=255)                                                                                 tGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAgcgc                                     >  1:306182/1‑140 (MQ=255)                                                                                 tGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAgcgc                                     >  1:381359/1‑140 (MQ=255)                                                                                 tGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAg                                        >  2:2643/1‑137 (MQ=255)                                                                                 tGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAg                                        <  1:2643/137‑1 (MQ=255)                                                                                   cGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCt                                   <  2:666767/140‑1 (MQ=255)                                                                                         cGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGcc                             <  2:430684/140‑1 (MQ=255)                                                                                         cGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGcc                             >  1:987019/1‑140 (MQ=255)                                                                                         cGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGcc                             <  2:381359/140‑1 (MQ=255)                                                                                         cGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGcc                             <  2:306182/140‑1 (MQ=255)                                                                                           gAAAACGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAg                           <  1:875194/140‑1 (MQ=255)                                                                                              aaCGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGcc                        <  1:1117739/140‑1 (MQ=255)                                                                                              aaCGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGcc                        <  1:1259524/140‑1 (MQ=255)                                                                                              aaCGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGcc                        <  2:135697/140‑1 (MQ=255)                                                                                              aaCGCGCGACGAAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGcc                        <  2:845245/140‑1 (MQ=255)                                                                                                      acgaAATCGACCAACGCGTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAg                >  1:737776/1‑140 (MQ=255)                                                                                                                    cgcgTTGATGGCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCaa  >  1:681509/1‑140 (MQ=255)                                                                                                                              |                                                                                                                                  ACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCAAAGTCCTGCCCGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAA  >  NC_000913/2565597‑2565851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑