Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,740,658	C→T	A165T (GCT→ACT)	aroF ←	3‑deoxy‑7‑phosphoheptulonate synthase, Tyr‑sensitive

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,740,658	0	C	T	100.0%	314.0 / NA	90	A165T (GCT→ACT)	aroF	3‑deoxy‑7‑phosphoheptulonate synthase, Tyr‑sensitive
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (41/49);  total (41/49)

AAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTAC  >  NC_000913/2740522‑2740793                                                                                                                                         |                                                                                                                                        aaaCGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTAcc                                                                                                                                      <  1:528456/140‑1 (MQ=255)  aaCGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCa                                                                                                                                     >  1:262102/1‑140 (MQ=255)   aCGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCaa                                                                                                                                    >  1:82740/1‑140 (MQ=255)    cGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAAt                                                                                                                                   >  2:804206/1‑140 (MQ=255)         cGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTg                                                                                                                              <  2:2523/140‑1 (MQ=255)         cGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTg                                                                                                                              <  2:5327/140‑1 (MQ=255)         cGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTg                                                                                                                              <  2:843299/140‑1 (MQ=255)         cGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTg                                                                                                                              >  1:5327/1‑140 (MQ=255)          ggCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGa                                                                                                                             >  2:465390/1‑140 (MQ=255)          ggCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGa                                                                                                                             >  2:13192/1‑140 (MQ=255)           gCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGAc                                                                                                                            <  2:1074700/140‑1 (MQ=255)           gCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGAc                                                                                                                            <  2:262102/140‑1 (MQ=255)           gCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGAc                                                                                                                            <  1:274539/140‑1 (MQ=255)           gCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGAc                                                                                                                            <  2:1262006/140‑1 (MQ=255)           gCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGAc                                                                                                                            <  1:609665/140‑1 (MQ=255)            cTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGAcc                                                                                                                           >  1:285969/1‑140 (MQ=255)               ggcggcggcGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGc                                                                                                                        >  1:626959/1‑140 (MQ=255)                 cggcggcgCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTAcc                                                                                                                                      <  1:326811/124‑1 (MQ=255)                 cggcggcgCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTa                                                                                                                      <  2:82740/140‑1 (MQ=255)                 cggcggcgCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTa                                                                                                                      >  1:304366/1‑140 (MQ=255)                 cggcggcgCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTa                                                                                                                      >  2:317687/1‑140 (MQ=255)                 cagcggcgCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTAcc                                                                                                                                      >  2:326811/3‑124 (MQ=255)                      gcgcgcATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAg                                                                                                                 <  1:385000/140‑1 (MQ=255)                         cgcATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATc                                                                                                              >  1:337993/1‑140 (MQ=255)                         cgcATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATc                                                                                                              <  2:337993/140‑1 (MQ=255)                         cgcATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATc                                                                                                              >  2:375285/1‑140 (MQ=255)                          gcATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCg                                                                                                             <  2:28113/140‑1 (MQ=255)                           cATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGc                                                                                                            <  1:208785/140‑1 (MQ=255)                           cATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGc                                                                                                            <  1:20154/140‑1 (MQ=255)                              aGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCa                                                                                                         >  1:1236334/1‑140 (MQ=255)                                   tAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTAt                                                                                                    >  2:167757/1‑140 (MQ=255)                                     aTTGCTGTTGCCAGCCTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGTGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTg                                                                                                  <  2:830144/140‑1 (MQ=255)                                     aTTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTg                                                                                                  <  2:1095729/140‑1 (MQ=255)                                       tGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCg                                                                                                <  2:677206/140‑1 (MQ=255)                                       tGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCg                                                                                                <  2:1236334/140‑1 (MQ=255)                                         cTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCggg                                                                                              <  1:465390/140‑1 (MQ=255)                                         cTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCggg                                                                                              <  1:1212885/140‑1 (MQ=255)                                         cTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCggg                                                                                              <  1:1136216/140‑1 (MQ=255)                                          tGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGc                                                                                             >  2:838354/1‑140 (MQ=255)                                          tGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGc                                                                                             >  1:753225/1‑140 (MQ=255)                                            ttGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTa                                                                                           >  1:583575/1‑140 (MQ=255)                                            ttGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTa                                                                                           >  1:830005/1‑140 (MQ=255)                                              gCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCgg                                                                                               <  2:668726/134‑1 (MQ=255)                                              gCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCgg                                                                                               >  1:668726/1‑134 (MQ=255)                                              gCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTAtt                                                                                         <  1:443781/140‑1 (MQ=255)                                              gCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCAATTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTAtt                                                                                         <  2:1081975/140‑1 (MQ=255)                                                cAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCg                                                                                       <  1:255765/140‑1 (MQ=255)                                                       ccGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTaa                                                                                >  2:191079/1‑140 (MQ=255)                                                        cGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAAc                                                                               >  1:226826/1‑140 (MQ=255)                                                        cGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAAc                                                                               >  1:417143/1‑140 (MQ=255)                                                              tGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTcc                                                                         <  2:417143/140‑1 (MQ=255)                                                               gCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCg                                                                        <  2:255504/140‑1 (MQ=255)                                                                  gTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCg                                                                     <  1:979813/140‑1 (MQ=255)                                                                     tttAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCa                                                                  <  1:167757/140‑1 (MQ=255)                                                                         aaaCCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTgg                                                              <  1:317687/140‑1 (MQ=255)                                                                         aaaCCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTgg                                                              <  2:582273/140‑1 (MQ=255)                                                                         aaaCCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTgg                                                              <  1:766114/140‑1 (MQ=255)                                                                             caaccGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAG‑GGCAGTc                                                         <  1:376613/140‑1 (MQ=255)                                                                               accGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTcc                                                        >  1:501045/1‑140 (MQ=255)                                                                               accGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTcc                                                        >  2:737945/1‑140 (MQ=255)                                                                                      tGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTCCCATATTc                                                 >  1:858856/1‑140 (MQ=255)                                                                                         aaaGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTCCCATATTCAcc                                              <  2:226826/140‑1 (MQ=255)                                                                                             tccGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTCCCATATTCACCAGCt                                          >  2:350761/2‑140 (MQ=255)                                                                                             cccGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTCCCATATTCACCAGCt                                          >  1:588517/1‑140 (MQ=255)                                                                                              ccGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCGATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTCCCATATTCACCAGCTc                                         >  2:405486/1‑140 (MQ=255)                                                                                                 gAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTTGGATCTAACGCTTCCGTCGCCAGTGGCAGTCCCATATTCACCAGCTCAag                                      >  1:441365/1‑140 (MQ=255)                                                                                                 gAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTCCCATATTCACCAGCTCAag                                      <  1:278654/140‑1 (MQ=255)                                                                                                    gCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTCCCATATTCACCAGCTCAagcag                                   <  2:858856/140‑1 (MQ=255)                                                                                                    gCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTCCCATATTCACCAGCTCAagcag                                   <  2:583575/140‑1 (MQ=255)                                                                                                       aTTTCACGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGCGGCAGTCCCATATTCACCAGCTCAAGCAGCaa                                >  1:269052/1‑140 (MQ=255)                                                                                                            aCGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTCCCATATTCACCAGCTCAAGCAGCAATTTAc                           <  1:350761/140‑1 (MQ=255)                                                                                                            aCGGTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTCCCATATTCACCAGCTCAAGCAGCAATTTAc                           <  2:753225/140‑1 (MQ=255)                                                                                                              ggTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTCCCATATTCACCAGCTCAAGCAGCAATTTAcgc                         >  2:46021/1‑140 (MQ=255)                                                                                                              ggTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTCCCATATTCACCAGCTCAAGCAGCAATTTAcgc                         >  1:601565/1‑140 (MQ=255)                                                                                                               gTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTCCCATATTCACCAGCTCAAGCAGCAATTTAcgcg                        >  2:531686/1‑140 (MQ=255)                                                                                                               gTGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTCCCATATTCACCAGCTCAAGCAGCAATTTAcgcg                        <  1:737945/140‑1 (MQ=255)                                                                                                                tGAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTCCCATATTCACCAGCTCAAGCAGCAATTTAcgcgc                       >  2:921126/1‑140 (MQ=255)                                                                                                                 gAGTTTGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTCCCATATTCACCAGCTCAAGCAGCAATTTAcgcgcg                      <  2:626959/140‑1 (MQ=255)                                                                                                                     ttGCGATTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCt                  >  2:294646/1‑140 (MQ=255)                                                                                                                          aTTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGc             <  2:601565/140‑1 (MQ=255)                                                                                                                          aTTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGc             <  1:838354/140‑1 (MQ=255)                                                                                                                          aTTCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGc             >  1:301538/1‑140 (MQ=255)                                                                                                                           ttCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGcc            <  1:531686/140‑1 (MQ=255)                                                                                                                           ttCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGcc            <  1:375285/140‑1 (MQ=255)                                                                                                                            tCCGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGccc           >  1:1211163/1‑140 (MQ=255)                                                                                                                              cGTTGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCgg         >  2:147678/1‑140 (MQ=255)                                                                                                                                 tGTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCGGCtt      <  1:294646/140‑1 (MQ=255)                                                                                                                                  gTACGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGCGGCAGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTc     <  2:269052/140‑1 (MQ=255)                                                                                                                                     cGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTAc  >  2:464938/1‑140 (MQ=255)                                                                                                                                     cGAGTACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTAc  >  2:814789/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        AAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTAC  >  NC_000913/2740522‑2740793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑