Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,762,824	T→C	K239K (AAA→AAG)	abpB ←	CP4‑57 prophage; putative helicase YfjK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,762,824	0	T	C	100.0%	185.7 / NA	53	K239K (AAA→AAG)	abpB	CP4‑57 prophage; putative helicase YfjK
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/23);  total (30/23)

CGTCTTAACATCTAGCGCGACTGTATTGAAGTCGGTAGAAACGAAAGTATGCGGATATCCAAGTTTTTCGAGACCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGTTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTCTTGATGATCAATGTCCCCGCTTTTCAGTTGTCGGAACGCCAACTTGTAAAACTCGTCAATGACGAAGAGGTCAATGTCGACGATGTCGTCACGCTCG  >  NC_000913/2762692‑2762959                                                                                                                                     |                                                                                                                                        cGTCTTAACATCTAGCGCGACTGTATTGAAGTCGGTAGAAACGAAAGTATGCGGATATCCAAGTTTTTCGAGACCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTa                                                                                                                                  <  2:328160/140‑1 (MQ=255)              aGCGCGACTGTATTGAAGTCGGTAGAAACGAAAGTATGCGGATATCCAAGTTTTTCGAGACCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTa                                                                                                                                  <  2:645223/127‑1 (MQ=255)              aGCGCGACTGTATTGAAGTCGGTAGAAACGAAAGTATGCGGATATCCAAGTTTTTCGAGACCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTa                                                                                                                                  >  1:645223/1‑127 (MQ=255)              aGCGCGACTGTATTGAAGTCGGTAGAAACGAAAGTATGCGGATATCCAAGTTTTTCGAGACCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAg                                                                                                                     >  1:1025640/1‑140 (MQ=255)              aGCGCGACTGTATTGAAGTCGGTAGAAACGAAAGTATGCGGATATCCAAGTTTTTCGAGACCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAg                                                                                                                     >  2:753334/1‑140 (MQ=255)              aGCGCGACTGTATTGAAGTCGGTAGAAACGAAAGTATGCGGATATCCAAGTTTTTCGAGACCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAg                                                                                                                     >  1:286202/1‑140 (MQ=255)              aGCGCGACTGTATTGAAGTCGGTAGAAACGAAAGTATGCGGATATCCAAGTTTTTCGAGACCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAg                                                                                                                     >  1:644978/1‑140 (MQ=255)               gcgcGACTGTATTGAAGTCGGTAGAAACGAAAGTATGCGGATATCCAAGTTTTTCGAGACCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAg                                                                                                                     >  2:415839/1‑139 (MQ=255)               gcgcGACTGTATTGAAGTCGGTAGAAACGAAAGTATGCGGATATCCAAGTTTTTCGAGACCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAg                                                                                                                     <  1:415839/139‑1 (MQ=255)                cgcgACTGTATTGAAGTCGGTAGAAACGAAAGTATGCGGATATCCAAGTTTTTCGAGACCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCt                                                                                                                   >  1:986141/1‑140 (MQ=255)                 gcgACTGTATTGAAGTCGGTAGAAACGAAAGTATGCGGATATCCAAGTTTTTCGAGACCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTc                                                                                                                  >  2:623427/1‑140 (MQ=255)                    aCTGTATTGAAGTCGGTAGAAACGAAAGTATGCGGATATCCAAGTTTTTCGAGACCGCGAATTCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGAt                                                                                                               >  1:596185/1‑140 (MQ=255)                            gAAGTCGGTAGAAACGAAAGTATGCGGATATCCAAGTTTTTCGAGACCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACtcgtt                                                                                                       <  2:190045/140‑1 (MQ=255)                              aGTCGGTAGAAACGAAAGTATGCGGATATCCAAGTTTTTCGAGACCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACtcgttcg                                                                                                     <  2:986141/140‑1 (MQ=255)                               gTCGGTAGAAACGAAAGTATGCGGATATCCAAGTTTTTCGAGACCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACtcgttcgt                                                                                                    >  1:491616/1‑140 (MQ=255)                               gTCGGTAGAAACGAAAGTATGCGGATATCCAAGTTTTTCGAGACCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACtcgttcgt                                                                                                    >  1:493623/1‑140 (MQ=255)                                tCGGTAGAAACGAAAGTATGCGGATATCCAAGTTTTTCGAGACCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTc                                                                                                   <  2:493623/140‑1 (MQ=255)                                tCGGTAGAAACGAAAGTATGCGGATATCCAAGTTTTTCGAGACCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTc                                                                                                   <  1:623427/140‑1 (MQ=255)                                tCGGTAGAAACGAAAGTATGCGGATATCCAAGTTTTTCGAGACCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTc                                                                                                   >  1:1196460/1‑140 (MQ=255)                                  ggTAGAAACGAAAGTATGCGGATATCCAAGTTTTTCGAGACCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACtcgttcg                                                                                                     <  2:949504/136‑1 (MQ=255)                                  ggTAGAAACGAAAGTATGCGGATATCCAAGTTTTTCGAGACCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACtcgttcg                                                                                                     >  1:949504/1‑136 (MQ=255)                                    tAGAAACGAAAGTATGCGGATATCCAAGTTTTTCGAGACCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTCTtga                                                                                               <  1:1249005/140‑1 (MQ=255)                                                 aTGCGGATATCCAAGTTTTTCGAGACCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTCTTGATGATCAATGTccc                                                                                  >  2:556834/1‑140 (MQ=255)                                                  tGCGGATATCCAAGTTTTTCGAGACCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTCTTGATGATCAATGTcccc                                                                                 >  1:1203393/1‑140 (MQ=255)                                                  tGCGGATATCCAAGTTTTTCGAGACCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTCTTGATGATCAATGTcccc                                                                                 >  1:286171/1‑140 (MQ=255)                                                   gCGGATATCCAAGTTTTTCGAGACCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTCTTGATGATCAATGTCCCCg                                                                                <  2:1203393/140‑1 (MQ=255)                                                     ggATATCCAAGTTTTTCGAGACCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTCTTGATGATCAATGTCCCCGCt                                                                              >  2:524227/1‑140 (MQ=255)                                                        tatCCAAGTTTTTCGAGACCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTCTTGATGATCAATGTCCCCGCtttt                                                                           <  1:524227/140‑1 (MQ=255)                                                          tCCAAGTTTTTCGAGACCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTCTTGATGATCAATGTCCCCGCtttt                                                                           <  1:1016922/138‑1 (MQ=255)                                                          tCCAAGTTTTTCGAGACCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTCTTGATGATCAATGTCCCCGCtttt                                                                           >  2:1016922/1‑138 (MQ=255)                                                          tCCAAGTTTTTCGAGACCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTCTTGATGATCAATGTCCCCGCTTTTCa                                                                         >  2:289994/1‑140 (MQ=255)                                                              aGTTTTTCGAGACCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTCTTGATGATCAATGTCCCCGCTTTTCAGTTg                                                                     >  1:440735/1‑140 (MQ=255)                                                                     cGAGACCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTCTTGATGATCAATGTCCCCGCTTTTCAGTTGTCGGAAc                                                              >  1:772786/1‑140 (MQ=255)                                                                      gagaCCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTCTTGATGATCAATGTCCCCGCTTTTCAGTTGTCGGAACg                                                             <  2:772786/140‑1 (MQ=255)                                                                        gaCCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTCTTGATGATCAATGTCCCCGCTTTTCAGTTGTCGGAACGcc                                                           <  1:753334/140‑1 (MQ=255)                                                                        gaCCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTCTTGATGATCAATGTCCCCGCTTTTCAGTTGTCGGAACGcc                                                           <  2:459037/140‑1 (MQ=255)                                                                         aCCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTCTTGATGATCAATGTCCCCGCTTTTCAGTTGTCGGAACGCCa                                                          <  2:286202/140‑1 (MQ=255)                                                                         aCCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTCTTGATGATCAATGTCCCCGCTTTTCAGTTGTCGGAACGCCa                                                          <  2:440735/140‑1 (MQ=255)                                                                             cgAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTCTTGATGATCAATGTCCCCGCTTTTCAGTTGTCGGAACGCCAACtt                                                      >  2:354657/1‑140 (MQ=255)                                                                                 tGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTCTTGATGATCAATGTCCCCGCTTTTCAGTTGTCGGAACGCCAACTTGTaa                                                  <  2:491616/140‑1 (MQ=255)                                                                                      ttAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTCTTGATGATCAATGTCCCCGCTTTTCAGTTGTCGGAACGCCAACTTGTAAAACTc                                             >  2:559709/1‑140 (MQ=255)                                                                                        aacgaacgGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTCTTGATGATCAATGTCCCCGCTTTTCAGTTGTCGGAACGCCAACTTGTAAAACTCGt                                           <  1:289994/140‑1 (MQ=255)                                                                                        aacgaacgGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTCTTGATGATCAATGTCCCCGCTTTTCAGTTGTCGGAACGCCAACTTGTAAAACTCGt                                           <  1:559709/140‑1 (MQ=255)                                                                                              cgGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTCTTGATGATCAATGTCCCCGCTTTTCAGTTGTCGGAACGCCAACTTGTAAAACTCGTCAATg                                      <  1:990388/139‑1 (MQ=255)                                                                                              cgGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTCTTGATGATCAATGTCCCCGCTTTTCAGTTGTCGGAACGCCAACTTGTAAAACTCGTCAATg                                      >  2:990388/1‑139 (MQ=255)                                                                                                gTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTCTTGATGATCAATGTCCCCGCTTTTCAGTTGTCGGAACGCCAACTTGTAAAACTCGTCAATGACg                                   >  2:990582/1‑140 (MQ=255)                                                                                                          gTAAAACTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTCTTGATGATCAATGTCCCCGCTTTTCAGTTGTCGGAACGCCAACTTGTAAAACTCGTCAATGACGAAGAGGTCaa                         <  2:1025640/140‑1 (MQ=255)                                                                                                             aaaCTGCCTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTCTTGATGATCAATGTCCCCGCTTTTCAGTTGTCGGAACGCCAACTTGTAAAACTCGTCAATGACGAAGAGGTCAATGt                      >  1:143660/1‑140 (MQ=255)                                                                                                                   ccTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTCTTGATGATCAATGTCCCCGCTTTTCAGTTGTCGGAACGCCAACTTGTAAAACTCGTCAATGACGAAGAGGTCAATGTcgacga                >  2:102203/1‑140 (MQ=255)                                                                                                                    cTGGAGACCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTCTTGATGATCAATGTCCCCGCTTTTCAGTTGTCGGAACGCCAACTTGTAAAACTCGTCAATGACGAAGAGGTCAATGTCGACGAt               >  1:571624/1‑140 (MQ=255)                                                                                                                        agaCCTTAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTCTTGATGATCAATGTCCCCGCTTTTCAGTTGTCGGAACGCCAACTTGTAAAACTCGTCAATGACGAAGAGGTCAATGTCGACGATgtcg           >  1:251662/1‑140 (MQ=255)                                                                                                                             ttAAGCAGCTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTCTTGATGATCAATGTCCCCGCTTTTCAGTTGTCGGAACGCCAACTTGTAAAACTCGTCAATGACGAAGAGGTCAATGTCGACGATGTCGTCACg      <  2:571624/140‑1 (MQ=255)                                                                                                                                 gCAGCTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTCTTGATGATCAATGTCCCCGCTTTTCAGTTGTCGGAACGCCAACTTGTAAAACTCGTCAATGACGAAGAGGTCAATGTCGACGATGTCGTCACGCTCg  >  1:345569/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                        CGTCTTAACATCTAGCGCGACTGTATTGAAGTCGGTAGAAACGAAAGTATGCGGATATCCAAGTTTTTCGAGACCGCGAATGCTGTTAACGAACGGTCCCGTCAGGTAAAACTGCCTGGAGACCTTAAGCAGTTTGCTTAACGCAATGTTGAGCTCGATGACTCGTTCGTCTTGATGATCAATGTCCCCGCTTTTCAGTTGTCGGAACGCCAACTTGTAAAACTCGTCAATGACGAAGAGGTCAATGTCGACGATGTCGTCACGCTCG  >  NC_000913/2762692‑2762959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑