Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,770,794	G→A	T38T (ACG→ACA)	yfjS →	CP4‑57 prophage; inner membrane lipoprotein YfjS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,770,794	0	G	A	100.0%	194.4 / NA	59	T38T (ACG→ACA)	yfjS	CP4‑57 prophage; inner membrane lipoprotein YfjS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/29);  total (30/29)

CTGATTTCAGGGAAAAAATAATGAAACGTAAAACACTACCTCTGCTGGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACGCCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTACCCGTGAGTCGCCCGATGGTTGCGAGGGTGGCAGT  >  NC_000913/2770661‑2770929                                                                                                                                      |                                                                                                                                        cTGATTTCAGGGAAAAAATAATGAAACGTAAAACACTACCTCTGCTGGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCcg                                                                                                                                   <  1:1171975/140‑1 (MQ=255) cTGATTTCAGGGAAAAAATAATGAAACGTAAAACACTACCTCTGCTGGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCcg                                                                                                                                   <  2:535992/140‑1 (MQ=255) cTGATTTCAGGGAAAAAATAATGAAACGTAAAACACTACCTCTGCTGGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCcg                                                                                                                                   <  2:1088437/140‑1 (MQ=255)           ggAAAAAATAATGAAACGTAAAACACTACCTCTGCTGGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCg                                                                                                                         >  2:698501/1‑140 (MQ=255)           ggAAAAAATAATGAAACGTAAAACACTACCTCTGCTGGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCg                                                                                                                         >  1:294673/1‑140 (MQ=255)                     atGAAACGTAAAACACTACCTCTGCTGGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAg                                                                                                               <  1:486014/140‑1 (MQ=255)                          aCGTAAAACACTACCTCTGCTGGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTc                                                                                                          <  2:796437/140‑1 (MQ=255)                               aaaCACTACCTCTGCTGGCGCTGGTTGCCACCTCTCTGTTTCTTAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGgat                                                                                                     <  2:1167473/140‑1 (MQ=255)                                  cacTACCTCTGCTGGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGgatgat                                                                                                  <  2:668824/140‑1 (MQ=255)                                     tACCTCTGCTGGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTc                                                                                               >  1:893825/1‑140 (MQ=255)                                     tACCTCTGCTGGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTc                                                                                               >  2:223457/1‑140 (MQ=255)                                      aCCTCTGCTGGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCa                                                                                              >  2:630097/1‑140 (MQ=255)                                       ccTCTGCTGGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAg                                                                                             >  1:536556/1‑140 (MQ=255)                                            gctgGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCgaag                                                                                        <  2:294673/140‑1 (MQ=255)                                            gctgGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCgaag                                                                                        >  2:859679/1‑140 (MQ=255)                                             ctgGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCgaaga                                                                                       >  1:592157/1‑140 (MQ=255)                                             ctgGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCgaaga                                                                                       >  1:608437/1‑140 (MQ=255)                                             ctgGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCgaaga                                                                                       <  1:602348/140‑1 (MQ=255)                                             ctgGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCgaaga                                                                                       >  2:1096459/1‑140 (MQ=255)                                                  gcTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGt                                                                                  >  1:120110/1‑140 (MQ=255)                                                     ggTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTcac                                                                               >  2:300412/1‑140 (MQ=255)                                                                ctctGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTtc                                                                    >  1:785499/1‑140 (MQ=255)                                                                ctctGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTtc                                                                    >  2:317221/1‑140 (MQ=255)                                                                     tttCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATc                                                               <  2:782080/140‑1 (MQ=255)                                                                       tCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCg                                                             >  2:160257/1‑140 (MQ=255)                                                                           aGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTg                                                                         >  1:680130/1‑124 (MQ=255)                                                                           aGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTg                                                                         <  2:680130/124‑1 (MQ=255)                                                                           aGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGtct                                                         >  1:338107/1‑140 (MQ=255)                                                                           aGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGtct                                                         <  1:249766/140‑1 (MQ=255)                                                                             cgcCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCa                                                       >  1:636454/1‑140 (MQ=255)                                                                             cgcCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCa                                                       >  1:631174/1‑140 (MQ=255)                                                                               ccTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCAcc                                                     >  2:914464/1‑140 (MQ=255)                                                                                   cGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGc                                                 <  1:753105/140‑1 (MQ=255)                                                                                   cGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGc                                                 <  1:859679/140‑1 (MQ=255)                                                                                     aTGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAg                                               <  2:893825/140‑1 (MQ=255)                                                                                      tGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAgg                                              <  2:631174/140‑1 (MQ=255)                                                                                        aCAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTc                                            <  1:914464/140‑1 (MQ=255)                                                                                          aGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCtt                                          >  1:1215438/1‑140 (MQ=255)                                                                                             aGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACg                                       <  1:1096459/140‑1 (MQ=255)                                                                                             aGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACg                                       <  1:904131/140‑1 (MQ=255)                                                                                                 atgaCCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTAcc                                   >  1:207165/1‑140 (MQ=255)                                                                                                  tgaCCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTAccc                                  >  2:960371/1‑140 (MQ=255)                                                                                                  tgaCCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTAccc                                  >  1:454484/1‑140 (MQ=255)                                                                                                       tCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTACCCGTGAg                             <  1:698501/140‑1 (MQ=255)                                                                                                        cAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTACCCGTGAGt                            <  2:1215438/140‑1 (MQ=255)                                                                                                          aaGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTACCCGTGAGTCg                          <  2:592157/140‑1 (MQ=255)                                                                                                            gCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTACCCGTGAGTCGcc                        <  1:960371/140‑1 (MQ=255)                                                                                                               aTCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTACCCGTGAGTCGCCCGa                     <  2:454484/140‑1 (MQ=255)                                                                                                                tCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTACCCGTGAGTCGCCCGAt                    <  2:338107/140‑1 (MQ=255)                                                                                                                tCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTACCCGTGAGTCGCCCGAt                    >  1:147719/1‑140 (MQ=255)                                                                                                                tCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTACCCGTGAGTCGCCCGAt                    >  1:491526/1‑140 (MQ=255)                                                                                                                       aTTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTACCCGTGAGTCGCCCGATGGTTGCg             <  1:160257/140‑1 (MQ=255)                                                                                                                        tttAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTACCCGTGAGTCGCCCGATGGTTGCGa            >  1:761672/1‑140 (MQ=255)                                                                                                                          tAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTACCCGTGAGTCGCCCGATGGTTGCGAgg          >  2:1042853/1‑140 (MQ=255)                                                                                                                            aGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTACCCGTGAGTCGCCCGATGGTTGCGAGGGt        <  1:300412/140‑1 (MQ=255)                                                                                                                             ggACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTACCCGTGAGTCGCCCGATGGTTGCGAGGGTg       <  2:761672/140‑1 (MQ=255)                                                                                                                             ggACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTACCCGTGAGTCGCCCGATGGTTGCGAGGGTg       <  2:207165/140‑1 (MQ=255)                                                                                                                                 cTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTACCCGTGAGTCGCCCGATGGTTGCGAGGGTGGCAg   >  2:681255/1‑140 (MQ=255)                                                                                                                                  tCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTACCCGTGAGTCGCCCGATGGTTGCGAGGGTGGCAGt  >  2:580671/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                        CTGATTTCAGGGAAAAAATAATGAAACGTAAAACACTACCTCTGCTGGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACGCCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTACCCGTGAGTCGCCCGATGGTTGCGAGGGTGGCAGT  >  NC_000913/2770661‑2770929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑