Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,967,224	T→C	E404G (GAA→GGA)	ptsP ←	phosphoenolpyruvate‑protein phosphotransferase PtsP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,967,224	0	T	C	100.0%	326.4 / NA	92	E404G (GAA→GGA)	ptsP	phosphoenolpyruvate‑protein phosphotransferase PtsP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (44/48);  total (44/48)

CTTCATGTTCCGGGCTTAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGAT  >  NC_000913/2967090‑2967360                                                                                                                                       |                                                                                                                                         cttcATGTTCCGGGCTTAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTgc                                                                                                                                     >  2:300082/1‑140 (MQ=255)     aTGTTCCGGGCTTAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTgcagca                                                                                                                                 <  2:787404/140‑1 (MQ=255)      tGTTCCGGGCTTAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGcagcag                                                                                                                                >  1:1261874/1‑140 (MQ=255)      tGTTCCGGGCTTAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGcagcag                                                                                                                                >  1:132234/1‑140 (MQ=255)         tCCGGGCTTAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTAc                                                                                                                             <  2:669718/140‑1 (MQ=255)          ccGGGCTTAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTAc                                                                                                                             <  2:345953/139‑1 (MQ=255)          ccGGGCTTAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTAc                                                                                                                             >  1:345953/1‑139 (MQ=255)            gggCTTAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCgg                                                                                                                          >  1:12103/1‑140 (MQ=255)              gCTTAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCt                                                                                                                        <  2:175543/140‑1 (MQ=255)              gCTTAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCt                                                                                                                        <  1:500686/140‑1 (MQ=255)                 tAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCg                                                                                                                     >  1:436965/1‑140 (MQ=255)                      cAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCg                                                                                                                <  1:947157/140‑1 (MQ=255)                        gCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGAc                                                                                                              <  1:864084/140‑1 (MQ=255)                         cATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTAc                                                                                                                             >  1:417355/1‑124 (MQ=255)                         cATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTAc                                                                                                                             <  2:417355/124‑1 (MQ=255)                           ttGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAg                                                                                                           <  1:300082/140‑1 (MQ=255)                              aGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGAc                                                                                                              >  2:372261/1‑134 (MQ=255)                              aGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGAc                                                                                                              <  1:372261/134‑1 (MQ=255)                              aGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCaa                                                                                                        >  1:19207/1‑140 (MQ=255)                                cATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAAtt                                                                                                      <  1:700292/140‑1 (MQ=255)                                cATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAAtt                                                                                                      <  1:62938/140‑1 (MQ=255)                                  tGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCa                                                                                                    <  1:1001037/140‑1 (MQ=255)                                   gACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCAc                                                                                                   <  2:946911/140‑1 (MQ=255)                                            aCGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGc                                                                                          <  2:365348/140‑1 (MQ=255)                                              gCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCa                                                                                        <  1:850411/140‑1 (MQ=255)                                              gCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCa                                                                                        >  1:1171498/1‑140 (MQ=255)                                              gCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCa                                                                                        >  1:520740/1‑140 (MQ=255)                                               cTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCAt                                                                                       >  2:1067295/1‑140 (MQ=255)                                               cTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCAt                                                                                       >  2:153698/1‑140 (MQ=255)                                                tCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATc                                                                                      >  2:342228/1‑140 (MQ=255)                                                tCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATc                                                                                      >  2:536353/1‑140 (MQ=255)                                                tCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATc                                                                                      >  2:67042/1‑140 (MQ=255)                                                tCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATc                                                                                      >  2:567667/1‑140 (MQ=255)                                                tCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATc                                                                                      >  2:83103/1‑140 (MQ=255)                                                        ttttAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCa                                                                              <  1:147544/140‑1 (MQ=255)                                                           tAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCg                                                                           <  2:132234/140‑1 (MQ=255)                                                                gcgcGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTa                                                                      <  2:766531/140‑1 (MQ=255)                                                                gcgcGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTa                                                                      <  1:67042/140‑1 (MQ=255)                                                                 cgcgGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAATGATCAGCGTCCTAc                                                                     >  2:897397/1‑140 (MQ=255)                                                                 cgcgGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTAc                                                                     >  1:602875/1‑140 (MQ=255)                                                                     ggTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATg                                                                 <  1:153698/140‑1 (MQ=255)                                                                     ggTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATg                                                                 <  1:1067295/140‑1 (MQ=255)                                                                       tAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATgca                                                               <  1:1052313/140‑1 (MQ=255)                                                                               cGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGaa                                                       <  1:390820/140‑1 (MQ=255)                                                                                gTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAg                                                      >  1:1203191/1‑140 (MQ=255)                                                                                gTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAg                                                      >  2:765248/1‑140 (MQ=255)                                                                                  cATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGa                                                        <  2:1183587/136‑1 (MQ=255)                                                                                  cATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGa                                                        >  1:1183587/1‑136 (MQ=255)                                                                                   aTCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCt                                                   >  1:1008204/1‑140 (MQ=255)                                                                                    tCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTg                                                  >  1:398686/1‑140 (MQ=255)                                                                                      ttCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGaa                                                <  2:1008204/140‑1 (MQ=255)                                                                                      ttCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGaa                                                >  2:56840/1‑140 (MQ=255)                                                                                           ccAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATcc                                           <  1:567667/140‑1 (MQ=255)                                                                                             aGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCcgc                                         >  1:295185/1‑140 (MQ=255)                                                                                              gACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCcgcg                                        <  2:520740/140‑1 (MQ=255)                                                                                               aCGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCg                                                         <  1:1192330/122‑1 (MQ=255)                                                                                               aCGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCg                                                         >  2:1192330/1‑122 (MQ=255)                                                                                               aCGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGAAATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATGAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCgcgc                                       >  2:1113914/1‑140 (MQ=255)                                                                                                   cTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAATGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCAt                                   <  1:897397/140‑1 (MQ=255)                                                                                                   cTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCAt                                   <  1:156747/140‑1 (MQ=255)                                                                                                   cTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCAt                                   <  1:212238/140‑1 (MQ=255)                                                                                                       gCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCAt                                   >  2:834547/1‑136 (MQ=255)                                                                                                       gCTCAATCTCTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCAt                                   <  1:834547/136‑1 (MQ=255)                                                                                                             tctcTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAggg                         >  1:120568/1‑140 (MQ=255)                                                                                                             tctcTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAggg                         >  1:248811/1‑140 (MQ=255)                                                                                                             tctcTTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAggg                         >  2:1001364/1‑140 (MQ=255)                                                                                                              ctctTCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGa                        <  1:1113914/140‑1 (MQ=255)                                                                                                                 ttCACTAATTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATcc                     >  1:1094370/1‑140 (MQ=255)                                                                                                                        aTTAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAgcgc              >  1:1042766/1‑140 (MQ=255)                                                                                                                         ttAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCa             <  2:436965/140‑1 (MQ=255)                                                                                                                         ttAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCa             <  1:258630/140‑1 (MQ=255)                                                                                                                         ttAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCa             <  2:295185/140‑1 (MQ=255)                                                                                                                         ttAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCa             <  1:83103/140‑1 (MQ=255)                                                                                                                          tAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCAc            <  2:1171498/140‑1 (MQ=255)                                                                                                                          tAGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCAc            <  1:536353/140‑1 (MQ=255)                                                                                                                           aGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAg                 >  1:109867/1‑134 (MQ=255)                                                                                                                           aGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAg                 <  2:109867/134‑1 (MQ=255)                                                                                                                           aGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCa             <  2:909367/138‑1 (MQ=255)                                                                                                                           aGCCGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCa             >  1:909367/1‑138 (MQ=255)                                                                                                                             ccGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAgc                <  1:79172/133‑1 (MQ=255)                                                                                                                             ccGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAgc                >  2:79172/1‑133 (MQ=255)                                                                                                                             ccGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTa         >  2:282990/1‑140 (MQ=255)                                                                                                                             ccGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTa         >  2:599861/1‑140 (MQ=255)                                                                                                                             ccGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTa         >  1:901265/1‑140 (MQ=255)                                                                                                                             ccGCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTa         >  1:177756/1‑140 (MQ=255)                                                                                                                               gCTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTAcc       <  2:602875/140‑1 (MQ=255)                                                                                                                                cTGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCa      >  2:1055000/1‑140 (MQ=255)                                                                                                                                 tGATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCAt     <  1:56840/140‑1 (MQ=255)                                                                                                                                  gATATCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATg    >  1:275757/1‑140 (MQ=255)                                                                                                                                    tatCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGAt  <  2:901265/140‑1 (MQ=255)                                                                                                                                    tatCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGAt  <  2:12103/140‑1 (MQ=255)                                                                                                                                    tatCCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGAt  <  2:323676/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         CTTCATGTTCCGGGCTTAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGAT  >  NC_000913/2967090‑2967360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑