Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,060,960	G→A	P37P (CCG→CCA)	scpA →	methylmalonyl‑CoA mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,060,960	0	G	A	97.4%	252.2 / ‑2.4	76	P37P (CCG→CCA)	scpA	methylmalonyl‑CoA mutase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (37/37);  total (38/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.09e-01

TTTGATTGCGTTTTACGGAGCAAATAATGTCTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCA  >  NC_000913/3060824‑3061095                                                                                                                                         |                                                                                                                                        tttGATTGCGTTTTACGGAGCAAATAATGTCTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTg                                                                                                                                      <  2:71072/140‑1 (MQ=255)   tGATTGCGTTTTACGGAGCAAATAATGTCTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGta                                                                                                                                    >  2:596374/1‑140 (MQ=255)     aTTGCGTTTTACGGAGCAAATAATGTCTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGtata                                                                                                                                  >  2:1207469/1‑140 (MQ=255)     aTTGCGTTTTACGGAGCAAATAATGTCTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGtata                                                                                                                                  >  2:277465/1‑140 (MQ=255)      ttGCGTTTTACGGAGCAAATAATGTCTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATAc                                                                                                                                 <  2:354776/140‑1 (MQ=255)         cGTTTTACGGAGCAAATAATGTCTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGa                                                                                                                              >  1:144631/1‑140 (MQ=255)              tACGGAGCAAATAATGTCTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCg                                                                                                                         <  2:1211194/140‑1 (MQ=255)                     cAAATAATGTCTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGa                                                                                                                  >  2:622796/1‑140 (MQ=255)                            tGTCTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTg                                                                                                           <  1:622796/140‑1 (MQ=255)                             gTCTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTgg                                                                                                          >  2:1203937/1‑140 (MQ=255)                             gTCTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTgg                                                                                                          >  2:217150/1‑140 (MQ=255)                             gTCTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTgg                                                                                                          >  1:732417/1‑140 (MQ=255)                               ctgaCGAGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAg                                                                                                        >  2:1142766/4‑140 (MQ=255)                               cTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTgg                                                                                                          <  2:499734/138‑1 (MQ=255)                               cTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTgg                                                                                                          >  1:499734/1‑138 (MQ=255)                                tAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAgg                                                                                                       >  2:1146282/1‑140 (MQ=255)                                tAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAgg                                                                                                       <  1:596374/140‑1 (MQ=255)                                 aaCGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGt                                                                                                      <  1:597677/140‑1 (MQ=255)                                   cGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGa                                                                                                    >  1:1086761/1‑140 (MQ=255)                                   cGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGa                                                                                                    >  2:44029/1‑140 (MQ=255)                                    gTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGAc                                                                                                   <  2:144631/140‑1 (MQ=255)                                     tGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACa                                                                                                  >  1:124249/1‑140 (MQ=255)                                       cAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAgg                                                                                                <  2:518166/140‑1 (MQ=255)                                        aGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGAc                                                                                                   >  2:139680/1‑136 (MQ=255)                                        aGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGAc                                                                                                   <  1:139680/136‑1 (MQ=255)                                               gCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGt                                                                                                      <  2:1205100/126‑1 (MQ=255)                                               gCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGt                                                                                                      >  1:1205100/1‑126 (MQ=255)                                               gCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTc                                                                                        >  1:112475/1‑140 (MQ=255)                                               gCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTc                                                                                        >  1:865839/1‑140 (MQ=255)                                               gCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTc                                                                                        >  1:344008/1‑140 (MQ=255)                                               gCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTc                                                                                        >  1:630895/1‑140 (MQ=255)                                                      cTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGttt                                                                                 >  2:767607/1‑140 (MQ=255)                                                        tGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTgc                                                                               >  1:908916/1‑140 (MQ=255)                                                        tGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTgc                                                                               >  1:576477/1‑140 (MQ=255)                                                         gCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTgcc                                                                              >  2:967150/1‑140 (MQ=255)                                                           caacaaGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTgccgc                                                                            <  2:576477/140‑1 (MQ=255)                                                                        aGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTgg                                                               >  1:127928/1‑140 (MQ=255)                                                                         gCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGc                                                              <  1:661063/140‑1 (MQ=255)                                                                         gCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGc                                                              >  2:1260993/1‑140 (MQ=255)                                                                          ccGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGcc                                                             >  1:657673/1‑140 (MQ=255)                                                                             tCGGGAGAAACCTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTCACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGCGGCCcgc                                                          <  2:274902/140‑1 (MQ=255)                                                                             tCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCcgc                                                          >  1:97507/1‑140 (MQ=255)                                                                             tCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCcgc                                                          >  1:595219/1‑140 (MQ=255)                                                                              cGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCcgcg                                                         <  1:1203937/140‑1 (MQ=255)                                                                              cGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCcgcg                                                         <  2:112475/140‑1 (MQ=255)                                                                              cGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCcgcg                                                         <  1:885268/140‑1 (MQ=255)                                                                              cGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCcgcg                                                         <  2:128095/140‑1 (MQ=255)                                                                                ggAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTg                                                       <  2:344008/140‑1 (MQ=255)                                                                                ggAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTg                                                       <  1:277465/140‑1 (MQ=255)                                                                                ggAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTg                                                       <  2:509757/140‑1 (MQ=255)                                                                                ggAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTg                                                       <  1:767607/140‑1 (MQ=255)                                                                                  agaAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGcc                                                     <  2:963701/140‑1 (MQ=255)                                                                                  agaAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGcc                                                     <  2:657673/140‑1 (MQ=255)                                                                                  agaAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGcc                                                     <  1:967150/140‑1 (MQ=255)                                                                                  agaAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGcc                                                     <  1:1260993/140‑1 (MQ=255)                                                                                  agaAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGcc                                                     <  1:1207469/140‑1 (MQ=255)                                                                                     aaaCTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACt                                                  <  2:124249/140‑1 (MQ=255)                                                                                          gTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGta                                             <  2:908916/140‑1 (MQ=255)                                                                                              aCTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATAcc                                         >  2:161397/1‑140 (MQ=255)                                                                                                 cGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGcc                                      <  2:127928/140‑1 (MQ=255)                                                                                                 cGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGcc                                      <  2:732417/140‑1 (MQ=255)                                                                                                 cGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGcc                                      <  1:729346/140‑1 (MQ=255)                                                                                                   cTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCa                                    >  1:945348/1‑140 (MQ=255)                                                                                                     ggTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAAc                                  >  2:1258703/1‑140 (MQ=255)                                                                                                         catcaAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTg                              >  1:852690/1‑140 (MQ=255)                                                                                                         catcaAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTg                              <  2:97507/140‑1 (MQ=255)                                                                                                          atcaAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTgg                             >  2:1261352/1‑140 (MQ=255)                                                                                                            caAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGAc                           <  1:1258703/140‑1 (MQ=255)                                                                                                            caAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGAc                           <  1:44029/140‑1 (MQ=255)                                                                                                                ccGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATc                       <  1:217150/140‑1 (MQ=255)                                                                                                                         ggATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTAt              <  1:161397/140‑1 (MQ=255)                                                                                                                           aTCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGc            >  2:336419/1‑140 (MQ=255)                                                                                                                            tCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCt           <  2:852690/140‑1 (MQ=255)                                                                                                                                  tCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGtttt     >  2:16428/1‑140 (MQ=255)                                                                                                                                   cAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGttttt    >  1:466658/1‑140 (MQ=255)                                                                                                                                    aaGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTc   <  2:595219/140‑1 (MQ=255)                                                                                                                                     aGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCa  >  2:1154925/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        TTTGATTGCGTTTTACGGAGCAAATAATGTCTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCA  >  NC_000913/3060824‑3061095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑