BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,078,994	C→T	G113S (GGC→AGC)	cmtB ←	mannitol‑specific PTS enzyme IIA component CmtB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,078,994	0	C	T	100.0%	375.3 / NA	104	G113S (GGC→AGC)	cmtB	mannitol‑specific PTS enzyme IIA component CmtB
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (59/45); total (59/45)

Only 100 of 104 total aligned reads displayed.

TCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCCAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTAATGTCAAAGACATCCCGGTTTTAAGCGCCCCACATTCCGGTCGCGCA  >  NC_000913/3078867‑3079130

tCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAatc                                                                                                                              >  1:752891/1‑140 (MQ=255)

   tGTAGAGGAGAGAGTAATTATCCGCGGTTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAatcggc                                                                                                                           >  1:1109035/1‑140 (MQ=255)

     tAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAatcggcat                                                                                                                         >  2:1136382/1‑140 (MQ=255)

     tAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAatcggcat                                                                                                                         >  2:260959/1‑140 (MQ=255)

     tAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAatcggcat                                                                                                                         <  1:593584/140‑1 (MQ=255)

       gaggagAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAatcggcatcg                                                                                                                       >  2:1117845/1‑140 (MQ=255)

          gagagaGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAAtcggcatcggca                                                                                                                    >  1:678507/1‑140 (MQ=255)

          gagagaGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAAtcggcatcggca                                                                                                                    >  1:191475/1‑140 (MQ=255)

            gagagTAATTATCCGCGGCTGATAATGTCCGCTGATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGgcagc                                                                                                                  >  2:1009890/1‑140 (MQ=255)

            gagagTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGgcagc                                                                                                                  >  1:1001673/1‑140 (MQ=255)

             agagTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGgcagca                                                                                                                 >  1:671627/1‑140 (MQ=255)

               agTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAatcggcatcggc                                                                                                                     <  2:830476/134‑1 (MQ=255)

               agTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAatcggcatcggc                                                                                                                     >  1:830476/1‑134 (MQ=255)

               agTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCaga                                                                                                               >  2:610750/1‑140 (MQ=255)

               agTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCaga                                                                                                               >  2:240090/1‑140 (MQ=255)

               agTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCaga                                                                                                               >  2:259882/1‑140 (MQ=255)

               agTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCaga                                                                                                               >  2:651646/1‑140 (MQ=255)

               agTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCaga                                                                                                               >  2:163074/1‑140 (MQ=255)

                 tAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAatcggcatcggc                                                                                                                     <  2:386517/132‑1 (MQ=255)

                 tAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAatcggcatcggc                                                                                                                     >  1:386517/1‑132 (MQ=255)

                    ttATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTc                                                                                                          >  2:1189844/1‑140 (MQ=255)

                      aTCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCg                                                                                                        <  2:191475/140‑1 (MQ=255)

                      aTCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCg                                                                                                        >  2:302970/1‑140 (MQ=255)

                         cgcgGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATg                                                                                                     >  1:824709/1‑140 (MQ=255)

                         cgcgGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATg                                                                                                     >  2:18598/1‑140 (MQ=255)

                          gcgGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGa                                                                                                    <  1:755486/140‑1 (MQ=255)

                           cgGCTGATAATGTCCGCTGATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAg                                                                                                   <  1:1009890/140‑1 (MQ=255)

                           cgGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAg                                                                                                   >  1:286834/1‑140 (MQ=255)

                           cgGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAg                                                                                                   >  2:1065374/1‑140 (MQ=255)

                            ggCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCg                                                                                                        <  2:690569/134‑1 (MQ=255)

                            ggCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCg                                                                                                        >  1:690569/1‑134 (MQ=255)

                            ggCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGt                                                                                                  <  2:678507/140‑1 (MQ=255)

                               tGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGtaat                                                                                               <  1:302970/140‑1 (MQ=255)

                                    aTGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGGTGAGtaatttaat                                                                                          <  1:240090/140‑1 (MQ=255)

                                    aTGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGtaatttaat                                                                                          <  1:651646/140‑1 (MQ=255)

                                    aTGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGtaatttaat                                                                                          <  1:610750/140‑1 (MQ=255)

                                        ccGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGt                                                                                      >  1:469277/1‑140 (MQ=255)

                                           cTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGt                                                                                      <  2:249568/137‑1 (MQ=255)

                                           cTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGt                                                                                      >  1:249568/1‑137 (MQ=255)

                                           cTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCg                                                                                   >  2:835847/1‑140 (MQ=255)

                                                    tttCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTcccc                                                                          <  1:512034/140‑1 (MQ=255)

                                                      tCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCgg                                                                        <  1:259882/140‑1 (MQ=255)

                                                      tCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCgg                                                                        >  2:916971/1‑140 (MQ=255)

                                                      tCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCgg                                                                        <  1:18598/140‑1 (MQ=255)

                                                      tCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCgg                                                                        >  2:1238253/1‑140 (MQ=255)

                                                       cTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGa                                                                       <  2:469277/140‑1 (MQ=255)

                                                           tgatgCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATT‑CCCGGAAAATa                                                                  <  1:260959/140‑1 (MQ=255)

                                                             atgCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATaa                                                                 <  1:1136382/140‑1 (MQ=255)

                                                                  gTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACAcc                                                            <  2:1091432/140‑1 (MQ=255)

                                                                  gTTAAGAGTTCTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACAcc                                                            <  1:862474/140‑1 (MQ=255)

                                                                    tAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACAcc                                                            <  2:536910/138‑1 (MQ=255)

                                                                    tAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACAcc                                                            >  1:536910/1‑138 (MQ=255)

                                                                     aaGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATaaa                                                                >  2:613031/1‑133 (MQ=255)

                                                                     aaGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATaaa                                                                <  1:613031/133‑1 (MQ=255)

                                                                     aaGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTg                                                         <  1:1189844/140‑1 (MQ=255)

                                                                        agTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTc                                                      >  2:589013/1‑140 (MQ=255)

                                                                         gttgttCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCa                                                     >  1:334253/1‑140 (MQ=255)

                                                                          ttgttCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATG‑GCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAg                                                   >  2:649634/1‑140 (MQ=255)

                                                                             ttCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCTAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTa                                                 >  1:918187/1‑140 (MQ=255)

                                                                             ttCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACAcc                                                            >  1:172436/1‑129 (MQ=255)

                                                                             ttCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACAcc                                                            <  2:172436/129‑1 (MQ=255)

                                                                             ttCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGt                                                  >  1:377253/1‑139 (MQ=255)

                                                                             ttCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGt                                                  <  2:377253/139‑1 (MQ=255)

                                                                             ttCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTa                                                 <  1:285355/140‑1 (MQ=255)

                                                                             ttCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTa                                                 >  1:343656/1‑140 (MQ=255)

                                                                             ttCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTa                                                 <  2:483735/140‑1 (MQ=255)

                                                                             ttCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTa                                                 <  1:982551/140‑1 (MQ=255)

                                                                             ttCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTa                                                 >  1:968985/1‑140 (MQ=255)

                                                                             ttCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTa                                                 >  2:178073/1‑140 (MQ=255)

                                                                             ttCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTa                                                 <  1:835847/140‑1 (MQ=255)

                                                                             ttCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTa                                                 >  2:324030/1‑140 (MQ=255)

                                                                             ttCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTa                                                 >  1:1205506/1‑140 (MQ=255)

                                                                               cGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACAcc                                                            >  1:1137088/1‑127 (MQ=255)

                                                                               cGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACAcc                                                            <  2:1137088/127‑1 (MQ=255)

                                                                                    aTTTCTTCTTCGCACAGTAATTCTCTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTAATGTCaa                                          <  1:1066496/140‑1 (MQ=255)

                                                                                      ttcttcttcGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATG‑GCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTAATGTCAAAGa                                       <  1:649634/140‑1 (MQ=255)

                                                                                       tcttcttcGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTAATGTCAAAGa                                       >  2:412100/1‑140 (MQ=255)

                                                                                           cttcGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTAATGTCAAAGACATc                                   <  1:412100/140‑1 (MQ=255)

                                                                                            ttcGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTAATGTCAAAGACATcc                                  >  1:751536/1‑140 (MQ=255)

                                                                                            ttcGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTAATGTCAAAGACATcc                                  <  2:1109035/140‑1 (MQ=255)

                                                                                             tcGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTAATGTCAAAGACATccc                                 >  1:1096675/1‑140 (MQ=255)

                                                                                             tcGCACAGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTAATGTCAAAGACATccc                                 >  2:916775/1‑140 (MQ=255)

                                                                                                   aGTAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTAATGTCAAAGACATCCCGGtttt                           >  2:574383/1‑140 (MQ=255)

                                                                                                     tAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTAATGTCAAAGACATCCCGGTTTTaa                         <  2:751536/140‑1 (MQ=255)

                                                                                                     tAATTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTAATGTCAAAGACATCCCGGTTTTaa                         >  2:1187710/1‑140 (MQ=255)

                                                                                                       aTTCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTAATGTCAAAGACATCCCGGTTTTAAgc                       <  1:163074/140‑1 (MQ=255)

                                                                                                        ttCACTTAACGCCTGAATAGCGCTAATGTGCTAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTAATGTCAAAGACATCCCGGTTTTAAgcg                      <  2:918187/140‑1 (MQ=255)

                                                                                                        ttCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTAATGTCAAAGACATCCCGGTTTTAAgcg                      <  1:178073/140‑1 (MQ=255)

                                                                                                        ttCACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTAATGTCAAAGACATCCCGGTTTTAAgcg                      >  1:1214463/1‑140 (MQ=255)

                                                                                                          cACTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTAATGTCAAAGACATCCCGGTTTTAAGCGcc                    <  1:589013/140‑1 (MQ=255)

                                                                                                           aCTTAACGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTAATGTCAAAGACATCCCGGTTTTAAGCGccc                   <  2:1205506/140‑1 (MQ=255)

                                                                                                             cttaCGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTAATGTCAAAGACATCCCGGTTTTAAGCGCCCca                 <  2:183340/137‑1 (MQ=255)

                                                                                                                 cGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTAATGTCAAAGACATCCCGGTTTTAAGCGCCCCACAtt             >  2:603106/1‑140 (MQ=255)

                                                                                                                 cGCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTAATGTCAAAGACATCCCGGTTTTAAGCGCCCCACAtt             <  1:324030/140‑1 (MQ=255)

                                                                                                                  gCCTGAATAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTAATGTCAAAGACATCCCGGTTTTAAGCGCCCCACATTc            >  2:388180/1‑140 (MQ=255)

                                                                                                                       aaTAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTAATGTCAAAGACATCCCGGTTTTAAGCGCCCCACATTCCGGTc       >  1:414780/1‑140 (MQ=255)

                                                                                                                       aaTAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTAATGTCAAAGACATCCCGGTTTTAAGCGCCCCACATTCCGGTc       >  1:48733/1‑140 (MQ=255)

                                                                                                                       aaTAGCGCTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTAATGTCAAAGACATCCCGGTTTTAAGCGCCCCACATTCCGGTc       <  2:1001673/140‑1 (MQ=255)

                                                                                                                           gcgcTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTAATGTCAAAGACATCCCGGTTTTAAGCGCCCCACATTCCGGTCgcgc   >  1:567572/1‑140 (MQ=255)

                                                                                                                            cgcTAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTAATGTCAAAGACATCCCGGTTTTAAGCGCCCCACATTCCGGTCGCGCa  <  2:48733/140‑1 (MQ=255)

TCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAATAGCGCCAATGTGCGAATCGGCATCGGCAGCAGAGAGTCCGATGAGTAATTTAATTGGTTCGTCATTCCCCGGAAAATAAACACCTTGTTCAAGTAATGTCAAAGACATCCCGGTTTTAAGCGCCCCACATTCCGGTCGCGCA  >  NC_000913/3078867‑3079130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`