Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,663,316	Δ1 bp	coding (2903/3114 nt)	mdtF →	multidrug efflux pump RND permease MdtF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,663,316	0	T	.	100.0%	356.6 / NA	83	coding (2903/3114 nt)	mdtF	multidrug efflux pump RND permease MdtF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (41/42);  total (41/42)

TCTACTTCCAGGTTGGTTTGCTGACCACCATCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGATGCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGTCATGGTGCCGGTTCTGGCGCGCAAAACGCGGTAGGTACCGGCGTGATGGGCGGGATGTTTGCCGCAA  >  NC_000913/3663181‑3663450                                                                                                                                        |                                                                                                                                       tCTACTTCCAGGTTGGTTTGCTGACCACCATCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGtt                                                                                                                                   >  2:1271399/1‑140 (MQ=255) tCTACTTCCAGGTTGGTTTGCTGACCACCATCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGtt                                                                                                                                   >  1:692477/1‑140 (MQ=255)  cTACTTCCAGGTTGGTTTGCTGACCACCATCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGttt                                                                                                                                  >  1:428312/1‑140 (MQ=255)     cTTCCAGGTTGGTTTGCTGACCACCATCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACg                                                                                                                               <  1:101162/140‑1 (MQ=255)     cTTCCAGGTTGGTTTGCTGACCACCATCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACg                                                                                                                               >  2:1168234/1‑140 (MQ=255)     cTTCCAGGTTGGTTTGCTGACCACCATCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACg                                                                                                                               >  2:482530/1‑140 (MQ=255)       tCCAGGTTGGTTTGCTGACCACCATCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGcc                                                                                                                             <  2:208842/140‑1 (MQ=255)       tCCAGGTTGGTTTGCTGACCACCATCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGcc                                                                                                                             <  1:1344975/140‑1 (MQ=255)        ccAGGTTGGTTTGCTGACCACCATCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGccc                                                                                                                            >  1:888018/1‑140 (MQ=255)          aGGTTGGTTTGCTGACCACCATCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCaa                                                                                                                          >  2:1124727/1‑140 (MQ=255)          aGGTTGGTTTGCTGACCACCATCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCaa                                                                                                                          >  1:1043237/1‑140 (MQ=255)          aGGTTGGTTTGCTGACCACCATCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCaa                                                                                                                          <  2:737391/140‑1 (MQ=255)           ggttggttTGCTGACCACCATCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCa                                                                                                                           >  2:412488/1‑138 (MQ=255)           ggttggttTGCTGACCACCATCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCa                                                                                                                           >  2:638894/1‑138 (MQ=255)           ggttggttTGCTGACCACCATCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCa                                                                                                                           <  1:638894/138‑1 (MQ=255)           ggttggttTGCTGACCACCATCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCa                                                                                                                           <  1:412488/138‑1 (MQ=255)           ggttggttTGCTGACCACCATCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAAt                                                                                                                         >  1:1313397/1‑140 (MQ=255)             ttggttTGCTGACCACCATCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATcc                                                                                                                       >  2:444792/1‑140 (MQ=255)             ttggttTGCTGACCACCATCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATcc                                                                                                                       >  2:820499/1‑140 (MQ=255)              tggttTGCTGACCACCATCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCt                                                                                                                      <  2:692477/140‑1 (MQ=255)                gttTGCTGACCACCATCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGcc                                                                                                                             >  1:127294/1‑131 (MQ=255)                gttTGCTGACCACCATCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGcc                                                                                                                             <  2:127294/131‑1 (MQ=255)                   tGCTGACCACCATCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCtgatga                                                                                                                 <  1:1168234/140‑1 (MQ=255)                   tGCTGACCACCATCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCtgatga                                                                                                                 <  2:1313397/140‑1 (MQ=255)                        accaccATCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGG‑AAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACctctc                                                                                                           <  2:224517/140‑1 (MQ=255)                         ccaccaTCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACctctc                                                                                                           <  1:42802/140‑1 (MQ=255)                          caccaTCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCtg                                                                                                                     >  1:802972/1‑129 (MQ=255)                          caccaTCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCtg                                                                                                                     <  2:802972/129‑1 (MQ=255)                          caccaTCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACctctct                                                                                                          <  1:280737/140‑1 (MQ=255)                          caccaTCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACctctct                                                                                                          <  2:545658/140‑1 (MQ=255)                          caccaTCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACctctct                                                                                                          >  2:1285448/1‑140 (MQ=255)                           accaTCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTg                                                                                                         >  2:1354112/1‑140 (MQ=255)                               tCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCt                                                                                                     >  1:1348302/1‑140 (MQ=255)                               tCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCt                                                                                                     <  2:1043237/140‑1 (MQ=255)                                  ggCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTa                                                                                                  <  2:1321353/140‑1 (MQ=255)                                  ggCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTa                                                                                                  >  1:1081119/1‑140 (MQ=255)                                      ttCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATtct                                                                                              <  1:463616/140‑1 (MQ=255)                                      ttCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATtct                                                                                              <  2:888631/140‑1 (MQ=255)                                       tCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATtctc                                                                                             >  2:807155/1‑140 (MQ=255)                                        ccGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCg                                                                                            <  1:834715/140‑1 (MQ=255)                                           ccAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCg                                                                                         <  1:143267/140‑1 (MQ=255)                                            cAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGt                                                                                        <  1:1001573/140‑1 (MQ=255)                                            cAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGt                                                                                        <  1:686044/140‑1 (MQ=255)                                                  cGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGcc                                                                                  >  2:50124/1‑140 (MQ=255)                                                   gCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCg                                                                                 <  1:1124727/140‑1 (MQ=255)                                                       tCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTCCCGCTgg                                                                             <  2:1346483/140‑1 (MQ=255)                                                           gATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTAt                                                                         <  2:1213843/140‑1 (MQ=255)                                                             ttGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCa                                                                       <  1:621596/140‑1 (MQ=255)                                                              tGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGc                                                                                >  1:930717/1‑130 (MQ=255)                                                              tGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGc                                                                                <  2:930717/130‑1 (MQ=255)                                                               gTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGt                                                                     <  1:50124/140‑1 (MQ=255)                                                               gTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGt                                                                     >  2:867662/1‑140 (MQ=255)                                                                   aaTTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGTCATg                                                                 >  2:628603/1‑140 (MQ=255)                                                                      ttGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATc                                                                        >  2:571862/1‑130 (MQ=255)                                                                      ttGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATc                                                                        <  1:571862/130‑1 (MQ=255)                                                                              gagaTGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGTCATGGTGCCGGTTCt                                                      >  1:8108/1‑140 (MQ=255)                                                                                         gaaagaaGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGTCATGGTGCCGGTTCTGGCGCGCaaaa                                           >  2:976956/1‑140 (MQ=255)                                                                                          aaagaaGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGTCATGGTGCCGGTTCTGGCGCGCa                                              <  2:668316/136‑1 (MQ=255)                                                                                          aaagaaGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGTCATGGTGCCGGTTCTGGCGCGCa                                              >  1:668316/1‑136 (MQ=255)                                                                                          aaagaaGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGTCATGGTGCCGGTTCTGGCGCGCAAAAc                                          <  1:867662/140‑1 (MQ=255)                                                                                               agGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGTCATGGTGCCGGTTCTGGCGCGCAAAACGCggt                                     <  2:1348302/140‑1 (MQ=255)                                                                                                gggAAAACGCCGATAGAGGCAATCATCGAANCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGTCATGGTGCCGGTTCTGGCGCGCAAAACGCggta                                    >  1:584859/1‑140 (MQ=255)                                                                                                gggAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGTCATGGTGCCGGTTCTGGCGCGCAAAACGCggtc                                    >  2:45450/1‑139 (MQ=255)                                                                                                gggAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGTCATGGTGCCGGTTCTGGCGCGCAAAACGCggta                                    >  2:1002453/1‑140 (MQ=255)                                                                                                gggAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGTCATGGTGCCGGTTCTGGCGCGCAAAACGCggta                                    >  1:1335406/1‑140 (MQ=255)                                                                                                     aaCGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGTCATGGTGCCGGTTCTGGCGCGCAAAACGCGGTAGGTAc                               <  2:1041850/140‑1 (MQ=255)                                                                                                     aaCGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGTCATGGTGCCGGTTCTGGCGCGCAAAACGCGGTAGGTAc                               >  2:756150/1‑140 (MQ=255)                                                                                                      aCGCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGTCATGGTGCCGGTTCTGGCGCGCAAAACGCGGTAGGTAcc                              >  1:798875/1‑140 (MQ=255)                                                                                                        gCCGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGTCATGGTGCCGGTTCTGGCGCGCAAAACGCGGTAGGTACCgg                            >  2:296517/1‑140 (MQ=255)                                                                                                          cGATAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGTCATGGTGCCGGTTCTGGCGCGCAAAACGCGGTAGGTACCGGCg                          >  1:1029279/1‑140 (MQ=255)                                                                                                            aTAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGTCATGGTGCCGGTTCTGGCGCGCAAAACGCGGTAGGTACCGGCGTg                        >  1:1294061/1‑140 (MQ=255)                                                                                                             tAGAGGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGTCATGGTGCCGGTTCTGGCGCGCAAAACGCGGTAGGTACCGGCGTGa                       <  2:584859/140‑1 (MQ=255)                                                                                                              agagGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGTCATGGTGCCGGTTCTGGCGCGCAAAACGCGGTAGGTACCGGCGTGAt                      <  1:1307321/140‑1 (MQ=255)                                                                                                               gagGCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGTCATGGTGCCGGTTCTGGCGCGCAAAACGCGGTAGGTACCGGCGTGATg                     <  2:52031/140‑1 (MQ=255)                                                                                                                  gCAATCATCGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGTCATGGTGCCGGTTCTGGCGCGCAAAACGCGGTAGGTACCGGCGTGATGGGc                  >  1:878989/1‑140 (MQ=255)                                                                                                                      tcatcGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGTCATGGTGCCGGTTCTGGCGCGCAAAACGCGGTAGGTACCGGCGTGATGGGCGGGa              >  1:734426/1‑140 (MQ=255)                                                                                                                        atcGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGTCATGGTGCCGGTTCTGGCGCGCAAAACGCGGTAGGTACCGGCGTGATGGGCGGGATg            <  2:645901/140‑1 (MQ=255)                                                                                                                          cGAAGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGTCATGGTGCCGGTTCTGGCGCGCAAAACGCGGTAGGTACCGGCGTGATGGGCGGGATGtt          <  2:878989/140‑1 (MQ=255)                                                                                                                             aGCGGCGCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGTCATGGTGCCGGTTCTGGCGCGCAAAACGCGGTAGGTACCGGCGTGATGGGCGGGATGTTTGc       <  1:1354112/140‑1 (MQ=255)                                                                                                                              gcggcgCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGTCATGGTGCCGGTTCTGGCGCGCAAAACGCGGTAGGTACCGGCGTGATGGGCGGGATGTTTGcc      >  2:1355643/1‑140 (MQ=255)                                                                                                                                ggcgCGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGTCATGGTGCCGGTTCTGGCGCGCAAAACGCGGTAGGTACCGGCGTGATGGGCGGGATGTTTGCCGc    >  1:282525/1‑140 (MQ=255)                                                                                                                                 gcgcGGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGTCATGGTGCCGGTTCTGGCGCGCAAAACGCGGTAGGTACCGGCGTGATGGGCGGGATGTTTGCCGCa   <  1:482530/140‑1 (MQ=255)                                                                                                                                  cgcgGA‑GCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGTCATGGTGCCGGTTCTGGCGCGCAAAACGCGGTAGGTACCGGCGTGATGGGCGGGATGTTTGCCGCaa  <  1:628603/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       TCTACTTCCAGGTTGGTTTGCTGACCACCATCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGATGCGTTTACGCCCAATCCTGATGACCTCTCTGGCCTTTATTCTCGGCGTGCTGCCGCTGGTTATCAGTCATGGTGCCGGTTCTGGCGCGCAAAACGCGGTAGGTACCGGCGTGATGGGCGGGATGTTTGCCGCAA  >  NC_000913/3663181‑3663450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑