Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,303,697	Δ1 bp	coding (92/165 nt)	yojO →	uncharacterized protein YojO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,303,696	0	G	.	100.0%	103.4 / NA	25	coding (91/165 nt)	yojO	uncharacterized protein YojO
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (3/22);  total (3/22)

AATAAGCCATTTTTATAGCCGCTAAGATATTAAAGGATGTGTCAAAGATGCATACCCCGATCGGGGTAAAACCTGTAGCAGGATCAAAAGAGTGGC‑GGGAAGCGT‑GGCAAAAACGGGCTTTTGCTCACATTTCAAATGGTTATAAATATATTTATATAGCGATTGATTCACCAGAGATATTTCTGCTGGTTTGCTCTCTCATTAGAATTTAACACTAAAAGAGCAGGTAAAATTGTCTGAATGTTCTTTAAGTTATTCATAAAGCAAA  >  NC_000913/2303559‑2303826                                                                                                                                            |                                                                                                                                   aataaGCCATTTTTATAGCCGCTAAGATATTAAAGGATGTGTCAAAGATGCATACCCCGATCGGGGTAAAACCTGTAGCAGGATCAAAAGAGTGGC‑GGGAAGCGT‑GGCAAAAACGGGCTTTTGCTCACATTTCAAATgtt                                                                                                                                  <  1:324814/140‑3 (MQ=255)    aaGCCATTTTTATAGCCGCTAAGATATTAAAGGATGTGTCAAAGATGCATACCCCGATCGGGGTAAAACCTGTAGCAGGATCAAAAGAGTGGC‑GGGAAGCGT‑GGCAAAAACGGGCTTTTGCTCACATTTCAAAT‑GTtata                                                                                                                              <  2:597870/140‑1 (MQ=255)    aaGCCATTTTTATAGCCGCTAAGATATTAAAGGATGTGTCAAAGATGCATACCCCGATCGGGGTAAAACCTGTAGCAGGATCAAAAGAGTGGC‑GGGAAGCGT‑GGCAAAAACGGGCTTTTGCTCACATTTCAAAT‑GTtata                                                                                                                              <  1:375247/140‑1 (MQ=255)    aaGCCATTTTTATAGCCGCTAAGATATTAAAGGATGTGTCAAAGATGCATACCCCGATCGGGGTAAAACCTGTAGCAGGATCAAAAGAGTGGC‑GGGAAGCGT‑GGCAAAAACGGGCTTTTGCTCACATTTCAAAT‑GTtata                                                                                                                              <  1:565413/140‑1 (MQ=255)    aaGCCATTTTTATAGCCGCTAAGATATTAAAGGATGTGTCAAAGATGCATACCCCGATCGGGGTAAAACCTGTAGCAGGATCAAAAGAGTGGC‑GGGAAGCGT‑GGCAAAAACGGGCTTTTGCTCACATTTCAAAT‑GTtata                                                                                                                              <  1:597725/140‑1 (MQ=255)     aGCCATTTTTATAGCCGCTAAGATATTAAAGGATGTGTCAAAGATGCATA‑CCCGATCGGGGTAAAACCTGTAGCAGGATCAAAAGAGTGGC‑GGGAAGCGT‑GGCAAAAACGGGCTTTTGCTCACATTTCAAAT‑GTTATaaa                                                                                                                            <  1:766062/140‑1 (MQ=255)      gCCATTTTTATAGCCGCTAAGATATTAAAGGATGTGTCAAAGATGCATACCCCGATCGGGGTAAAACCTGTAGCAGGATCAAAAGAGTGGC‑GGGAAGCGT‑GGCAAAAACGGGCTTTTGCTCACATTTCAAAT‑GTTATaaa                                                                                                                            <  1:201095/140‑1 (MQ=255)      gCCATTTTTATAGCCGCTAAGATATTAAAGGATGTGTCAAAGATGCATACCCCGATCGGGGTAAAACCTGTAGCAGGATCAAAAGAGTGGC‑GGGAAGCGT‑GGCAAAAACGGGCTTTTGCTCACATTTCAAAT‑GTTATaaa                                                                                                                            <  1:407573/140‑1 (MQ=255)        cATTTTTATAGCCGCTAAGATATTAAAGGATGTGTCAAAGATGCATACCCCGATCGGGGTAAAACCTGTAGCAGGATCAAAAGAGTGGC‑GGGAAGCGT‑GGCAAAAACGGGCTTTTGCTCACATTTCAAAT‑GTTATAAata                                                                                                                          <  2:632675/140‑1 (MQ=255)                  gCCGCTAAGATATTAAAGGATGTGTCAAAGATGCATACCCCGATCGGGGTAAAACCTGTAGCAGGATCAAAAGAGTGGC‑GGGAAGCGT‑GGCAAAAACGGGCTTTTGCTCACATTTCAAAT‑GTTATAAATATATTtatata                                                                                                                >  2:89484/1‑140 (MQ=255)                  gCCGCTAAGATATTAAAGGATGTGTCAAAGATGCATACCCCGATCGGGGTAAAACCTGTAGCAGGATCAAAAGAGTGGC‑GGGAAGCGT‑GGCAAAAACGGGCTTTTGCTCACATTTCAAAT‑GTTATAAATATATTtatata                                                                                                                <  1:89484/140‑1 (MQ=255)                     gCTAAGATATTAAAGGATGTGTCAAAGATGCATACCCCGATCGGGGTAAAACCTGTAGCAGGATCAAAAGAGTGGC‑GGGAGAAGC‑GGGCAAAACGCGCGGTTTTTCTCATTTTTAAT‑GTTTTTAATATATTtaaaaattg                                                                                                             >  2:713303/1‑133 (MQ=255)                          gATATTAAAGGATGTGTCAAAGATGCATACCCCGATCGGGGTAAAACCTGTAGCAGGATCAAAAGAGTGGC‑GGGAAGCGT‑GGCAAAAACGGGCTTTTGCTCACATTTCAAAT‑GTTATAAATATATTTATATAGCgattga                                                                                                        <  2:1247938/140‑1 (MQ=255)                           atatTAAAGGATGTGTCAAAGATGCATACCCCGATCGGGGTAAAACCTGTAGCAGGATCAAAAGAGTGGC‑GGGAAGCGT‑GGCAAAAACGGGCTTTTGCTCACATTTCAAAT‑GTTATAAATATATTTATATAGCgattgat                                                                                                       <  2:1320098/140‑1 (MQ=255)                                     aTGTGTCAAAGATGCATACCCCGATCGGGGTAAAACCTGTAGCAGGATCAAAAGAGTGGCGGGGAAGGTG‑GGGAAAAAAGGGGTTTT‑TTTACATTTTAAAT‑ATTATAAAAATATTTTTATATAGATTTATTTTCCAaaga                                                                                             >  2:1235089/1‑140 (MQ=255)                                        tgtCAAAGATGCATACCCCGATCGGGGTAAAACCTGTAGCAGGATCAAAAGAGTGGC‑GGGAAACGG‑GGGGAAAAAAGGGTTTTGTTCACCTTTTTAAT‑ATTATAAATATATTTTTATTATGATTGATTTACCAAAGaaat                                                                                          >  2:799022/1‑140 (MQ=255)                                         gtCAAAGATGCATACCCCGATCGGGGTAAAACCTGTAGCAGGATCAAAAGAGTGGC‑GGGGAAAGG‑GGGAAAAAAAGGCTTTTTCTCACACTTCTAA‑AATTTTAAAAAAATTTATATATCGATTTATTTACCACAGAAAtt                                                                                         >  2:6655/1‑140 (MQ=255)                                              aGATGCATACCCCGATCGGGGTAAAACCTGTAGCAGGATCAAAAGAGTGGC‑GGGGAGCGG‑GGGGAAAAAAGGCGTTTTTTCTCATTTTAAAT‑ATTATAAAAATATTTATATTATGATTTATTTACCAAAGATAttttttt                                                                                    >  2:1066291/1‑136 (MQ=255)                                                   cATACCCCGATCGGGGTAAAACCTGTAGCAGGATCAAAAGAGTGGC‑GGGGAGGGTGGGAAAAAAAGGGCTTTT‑TTCCCATTTTCAAAGTTTTTAAA‑ATAAATTTATAAAGATTTATTTACCAAAGATATTTttgtctctt                                                                               >  2:87280/1‑134 (MQ=255)                                                    aTACCCCGATCGGGGTAAAACCTGTAGCAGGATCAAAAGAGTGGC‑GGGGAGGGG‑GGGGAAAAAAAGCGTTTTCTTTCATTTTTAA‑AATTATAAAAATATTTATATATAGAATGATTTTCCACAGAAATTTTTTCTTGttt                                                                              >  2:977013/1‑140 (MQ=255)                                                      aCCCCGATCGGGGTAAAACCTGTAGCAGGATCAAAAGAGTGGC‑GGGAAGCGT‑GGCAAAAACGGGCTTTTGCTCACATTTCAAAT‑GTTATAAATATATTTATATAGCGATTGATTCACCAGAGATATTTCTGCTGGTTTGc                                                                            <  1:6655/140‑1 (MQ=255)                                                         ccGATCGGGGTAAAACCTGTAGCAGGATCAAAAGAGTGGC‑GGGAAGCGT‑GGCAAAAACGGGCTTTTGCTCACATTTCAAAT‑GTTATAAATATATTTATATAGCGATTGATTCACCAGAGATATTTCTGCTGGTTTGctct                                                                         <  1:1228385/140‑1 (MQ=255)                                                           gATCGGGGTAAAACCTGTAGCAGGATCAAAAGAGTGGC‑GGGAAGCGT‑GGCAAAAACGGGCTTTTGCTCACATTTCAAAT‑GTTATAAATATATTTATATAGCGATTGATTCACCAGAGATATTTCTGCTGGTTTGctctct                                                                       <  1:4211/140‑1 (MQ=255)                                                                        ccTGTAGCAGGATCAAAAGAGTGGC‑GGGAAGCGT‑GGCAAAAACGGGCTTTTGCTCACATTTCAAAT‑GTTATAAATATATTTATATAGCGATTGATTCACCAGAGATATTTCTGCTGGTTTGCTCTCTCATTAGAATTTaa                                                          <  1:803366/140‑1 (MQ=255)                                                                         cTGTAGCAGGATCAAAAGAGTGGC‑GGGAAGCGT‑GGCAAAAACGGGCTTTTGCTCACATTTCAAAT‑GTTATAAATATATTTATATAGCGATTGATTCACCAGAGATATTTCTGCTGGTTTGCTCTCTCATTAGAATTTAac                                                         <  2:111718/140‑1 (MQ=255)                                                                                 ggATCAAAAGAGTGGC‑GGGGAGCGG‑GGCAAAAAAAGGCTTTTGCTCACATTTCAAAT‑ATTATAAAAAAAATTTTATAAAGATTGATTTACCCGAGATATTTTTTTTTGTTTGTTTTCTCTTTATAATTTtaaaataaaaa                                                 >  2:851454/1‑139 (MQ=255)                                                                                  gATCAAAAGAGTGGC‑GGGAAGCGT‑GGCAAAAACGGGCTTTTGCTCACATTTCAAAT‑GTTATAAATATATTTATATAGCGATTGATTCACCAGAGATATTTCTGCTGGTTTGCTCTCTCATTAGAATTTAACACTAAAaga                                                <  1:977013/140‑1 (MQ=255)                                                                                            gTGGC‑GGGAAGCGT‑GGCAAAAACGGGCTTTTGCTCACATTTCAAAT‑GTTATAAATATATTTATATAGCGATTGATTCACCAGAGATATTTCTGCTGGTTTGCTCTCTCATTAGAATTTAACACTAAAAGAGCAGGTaaaa                                      <  1:632113/140‑1 (MQ=255)                                                                                                       gCGT‑GGCAAAAACGGGCTTTTGCTCACATTTCAAAT‑GTTATAAATATATTTATATAGCGATTGATTCACCAGAGATATTTCCGCTGGTTTGCTCTCTCATTAGAATTTAACACTAAAAGAGCAGGTAAAATTGTCTGAAt                            <  1:353100/140‑1 (MQ=255)                                                                                                          t‑GGCAAAAACGGGCTTTTGCTCACATTTCAAAT‑GTTATAAATATATTTATATAGCGATTGATTCACCAGAGATATTTCTGCTGGTTTGCTCTCTCATTAGAATTTAACACTAAAAGAGCAGGTAAAATTGTCTGaa                             <  2:1092128/136‑1 (MQ=255)                                                                                                          t‑GGCAAAAACGGGCTTTTGCTCACATTTCAAAT‑GTTATAAATATATTTATATAGCGATTGATTCACCAGAGATATTTCTGCTGGTTTGCTCTCTCATTAGAATTTAACACTAAAAGAGCAGGTAAAATTGTCTGaa                             >  1:1092128/1‑136 (MQ=255)                                                                                                               aaaaaCGGGCTTTTGCTCACATTTCAAAT‑GTTATAAATATATTTATATAGCGATTGATTCACCAGAGATATTTCTGCTGGTTTGCTCTCTCATTAGAATTTAACACTAAAAGAGCAGGTAAAATTGTCTGAATGTTCttt                     <  1:1235089/140‑1 (MQ=255)                                                                                                                                 acaTTTCAAAT‑GTTATAAATATATTTATATAGCGATTGATTCACCAGAGATATTTCTGCTGGTTTGCTCTCTCATTAGAATTTAACACTAAAAGAGCAGATAAAATTGTCTGAATGTTCTTTAAGTTATTCATAAAGCaa   <  1:1309321/140‑1 (MQ=255)                                                                                                                                  caTTTCAAAT‑GTTATAAATATATTTATATAGCGATTGATTCACCAGAGATATTTCTGCTGGTTTGCTCTCTCATTAGAATTTAACACTAAAAGAGCAGGTAAAATTGTCTGAATGTTCTTTAAGTTATTCATAAAGCaaa  >  2:160740/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                   AATAAGCCATTTTTATAGCCGCTAAGATATTAAAGGATGTGTCAAAGATGCATACCCCGATCGGGGTAAAACCTGTAGCAGGATCAAAAGAGTGGC‑GGGAAGCGT‑GGCAAAAACGGGCTTTTGCTCACATTTCAAATGGTTATAAATATATTTATATAGCGATTGATTCACCAGAGATATTTCTGCTGGTTTGCTCTCTCATTAGAATTTAACACTAAAAGAGCAGGTAAAATTGTCTGAATGTTCTTTAAGTTATTCATAAAGCAAA  >  NC_000913/2303559‑2303826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑