Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,606,330 (G)8→9 noncoding (72/87 nt) leuQ ← tRNA‑Leu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,606,3221.G94.4% 177.5 / 2.9 54noncoding (80/87 nt)leuQtRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base . (2/1);  new base G (23/28);  total (25/29)
Fisher's exact test for biased strand distribution p-value = 5.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.31e-01

TTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGA‑GG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCT  >  NC_000913/4606184‑4606447
                                                                                                                                            |                                                                                                                                
ttGATATCGCCTCGGTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGA‑g                                                                                                                                 <  2:209305/140‑1 (MQ=255)
      tCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGA‑gg‑gg‑g‑gg                                                                                                                        <  1:1066648/140‑1 (MQ=255)
          cTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGA‑gg‑gg‑g‑ggggac                                                                                                                    <  1:132980/140‑4 (MQ=255)
           tCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGA‑gg‑gg‑g‑ggggact                                                                                                                   >  1:1275310/1‑136 (MQ=255)
              tttGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGa                                                                                                                 <  2:716563/140‑1 (MQ=255)
              tttGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGa                                                                                                                 <  2:1016793/140‑1 (MQ=255)
               ttGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGaa                                                                                                                <  1:40008/140‑1 (MQ=255)
                  gTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAAccc                                                                                                             >  1:63299/1‑140 (MQ=255)
                   tGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAACAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAAcccc                                                                                                            <  2:63299/140‑1 (MQ=255)
                    ggagggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAAcccca                                                                                                           <  1:614413/136‑2 (MQ=255)
                    gCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAAccccc                                                                                                           <  1:259049/140‑1 (MQ=255)
                     cGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCACTATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCa                                                                                                          <  2:1275310/140‑1 (MQ=255)
                     cGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCa                                                                                                          <  1:588008/140‑1 (MQ=255)
                     cGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCa                                                                                                          <  1:493143/140‑1 (MQ=255)
                     cGAGGGGGGGGACCTGAACCCCCATGTCAGTAAGAACACTAACACCTGAAGCTAGCACGTCAACCAATTCCGACACCTCAGGCAAGTGCGAGCAATAGCAACG‑AGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCa                                                                                                          <  2:180626/140‑1 (MQ=18)
                            ggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGt                                                                                                   <  1:871875/140‑1 (MQ=255)
                               gACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAg                                                                                                <  2:754101/140‑1 (MQ=255)
                                     aaCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGAGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACt                                                                                          >  2:1306680/1‑140 (MQ=255)
                                       cccccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTaa                                                                                        <  2:1194171/140‑1 (MQ=255)
                                          ccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAacac                                                                                     >  1:330300/1‑140 (MQ=255)
                                           cgcGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACAcc                                                                                    <  1:424211/138‑1 (MQ=255)
                                            aCGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGCAGCGA‑GGCGC‑G‑TGGTCTTGAACCCCCACGCCCGTAAGGACACTAACAccg                                                                                   >  2:523729/1‑139 (MQ=25)
                                                ccGTAAGAACACTAACACCTGAATCCTGCGCGTCTACCGCTTCCGCCGTCTACGAGAAGTGCCAGCAATATAAGCGAAGATTTTTGGTCCGG‑GG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAg                                                                               <  2:807626/140‑1 (MQ=12)
                                                        acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGcgcg                                                                       >  2:500243/1‑140 (MQ=255)
                                                         cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTa                                                                            >  1:460445/1‑134 (MQ=255)
                                                         cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTa                                                                            <  2:460445/134‑1 (MQ=255)
                                                          acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGTCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTc                                                                     >  2:838043/1‑140 (MQ=255)
                                                              acacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGTCTTGGACCCCCACTTCCCTAAAGAAACTAACACCTGAAGCTAGCGCGATTAcc                                                                 >  2:415467/1‑140 (MQ=37)
                                                              acacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTAcc                                                                 >  1:1086183/1‑140 (MQ=255)
                                                                  cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGA‑GG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTc                                                            >  1:53658/1‑140 (MQ=255)
                                                                    gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                                                           >  1:122987/1‑140 (MQ=255)
                                                                    gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                                                           >  1:375870/1‑140 (MQ=255)
                                                                     aaGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                          >  2:1277657/1‑140 (MQ=255)
                                                                            cgcgTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                                                       >  1:685289/1‑136 (MQ=255)
                                                                            cgcgTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                                                       <  2:685289/136‑1 (MQ=255)
                                                                            cgcgTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt                                                   >  1:294960/1‑140 (MQ=255)
                                                                                tCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCa                                               <  1:500243/140‑1 (MQ=255)
                                                                                    ccAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATAcc                                           <  1:1306680/140‑1 (MQ=255)
                                                                                       aTTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATc                                        >  2:124043/1‑140 (MQ=255)
                                                                                         tCCGCCACCTTCGCCCAGTGCGAGCAATATCAATG‑TCCTCTTTGGTGGCA‑GG‑GGCG‑GAGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCaa                                      <  2:1089815/140‑1 (MQ=18)
                                                                                         tCCGCCACCTTCGCCCACTGCGAGCAATATCAATG‑TCCTCTTTGGCGGCA‑GG‑GG‑GAGGGGCTTGAAACCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCaa                                      <  2:325661/140‑1 (MQ=18)
                                                                                           cGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAAtt                                    >  1:632259/1‑140 (MQ=255)
                                                                                           cGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAAtt                                    >  1:1171722/1‑140 (MQ=255)
                                                                                              cACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCa                                               <  2:320874/126‑1 (MQ=255)
                                                                                              cACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCa                                               >  1:320874/1‑126 (MQ=255)
                                                                                                      cccAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGaa                         <  2:294960/140‑1 (MQ=255)
                                                                                                       ccAGTTCGAGCAATATCAACG‑TGGTCTTTGGTACGA‑GG‑GGCG‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATGCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAAt                        <  2:1207472/140‑1 (MQ=21)
                                                                                                           tGCGAGCAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCt                    >  1:509233/1‑140 (MQ=255)
                                                                                                           tGCGAGCAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCt                    >  1:544746/1‑140 (MQ=255)
                                                                                                             cGAGCAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTAc                  <  2:122987/140‑1 (MQ=255)
                                                                                                                 cAATATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACg              <  2:1086183/140‑1 (MQ=255)
                                                                                                                  aaTATCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACgg             <  1:523729/140‑1 (MQ=255)
                                                                                                                   atatCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGa            <  2:544746/140‑1 (MQ=255)
                                                                                                                    tatCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAg           >  2:121813/1‑140 (MQ=255)
                                                                                                                    tatCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAg           <  2:330300/140‑1 (MQ=255)
                                                                                                                     atCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAAt                        <  1:584305/126‑1 (MQ=255)
                                                                                                                     atCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAAt                        >  2:584305/1‑126 (MQ=255)
                                                                                                                     atCAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAgg          >  1:510867/1‑140 (MQ=255)
                                                                                                                     atCAACG‑TGGTTTTTGGTGCGA‑GG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgc         >  1:1283839/1‑140 (MQ=255)
                                                                                                                       cAACG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg        <  2:1171722/140‑1 (MQ=255)
                                                                                                                         aCG‑TGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCa      <  1:1277657/140‑1 (MQ=255)
                                                                                                                             tGGTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCAT‑AATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCt  >  1:656928/1‑140 (MQ=255)
                                                                                                                               gTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAg           >  2:481394/1‑130 (MQ=255)
                                                                                                                               gTTTTTGGTGCGAGGG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAg           <  1:481394/130‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                
TTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG‑TGGTTTTTGGTGCGA‑GG‑GG‑G‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCT  >  NC_000913/4606184‑4606447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑