Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,068,157	T→C	intergenic (‑77/+16)	yggE ← / ← argO	uncharacterized protein YggE/L‑arginine exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,068,157	0	T	C	100.0%	291.4 / NA	80	intergenic (‑77/+16)	yggE/argO	uncharacterized protein YggE/L‑arginine exporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/39);  total (41/39)

CTGCGCTGCCATCCCGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCA  >  NC_000913/3068021‑3068288                                                                                                                                         |                                                                                                                                    cTGCGCTGCCATCCCGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTcc                                                                                                                                  <  1:886155/140‑1 (MQ=255)         ccATCCCGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATa                                                                                                                          >  2:1087604/1‑140 (MQ=255)         ccATCCCGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATa                                                                                                                          >  2:1192801/1‑140 (MQ=255)            tCCCGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTAGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAgcg                                                                                                                       <  1:1192801/140‑1 (MQ=255)              ccGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGcc                                                                                                                     <  2:877826/140‑1 (MQ=255)               cGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCt                                                                                                                    <  1:571152/140‑1 (MQ=255)                 cTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTaa                                                                                                                  <  2:563251/140‑1 (MQ=255)                     tACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGa                                                                                                              >  2:117081/1‑140 (MQ=255)                      aCCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGaa                                                                                                             >  2:102231/1‑140 (MQ=255)                        ccATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACa                                                                                                           >  1:565614/1‑140 (MQ=255)                          aTTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAg                                                                                                         >  2:589167/1‑140 (MQ=255)                           ttAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAgg                                                                                                        >  1:1101873/1‑140 (MQ=255)                            tAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTaa                                                                                                                  <  1:1281540/129‑1 (MQ=255)                            tAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTaa                                                                                                                  >  2:1281540/1‑129 (MQ=255)                            tAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACa                                                                                                           <  1:650236/136‑1 (MQ=255)                            tAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACa                                                                                                           >  2:650236/1‑136 (MQ=255)                                gccgccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTtgt                                                                                                   >  1:271732/1‑140 (MQ=255)                                  cgccAGGGCGATAACTTTGAACTTCGCGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGc                                                                                                 <  1:697848/140‑1 (MQ=255)                                  cgccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGc                                                                                                 <  1:1294599/140‑1 (MQ=255)                                  cgccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGc                                                                                                 >  1:903583/1‑140 (MQ=255)                                   gccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTaa                                                                                                                  <  1:952731/122‑1 (MQ=255)                                   gccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTaa                                                                                                                  >  2:952731/1‑122 (MQ=255)                                     cAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATg                                                                                              >  1:700245/1‑140 (MQ=255)                                      aGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGa                                                                                             >  2:584383/1‑140 (MQ=255)                                       gggCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGc                                                                                                 <  1:564167/135‑1 (MQ=255)                                       gggCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGc                                                                                                 >  2:564167/1‑135 (MQ=255)                                       gggCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAg                                                                                            <  2:1101873/140‑1 (MQ=255)                                        ggCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGc                                                                                                 >  2:361816/1‑134 (MQ=255)                                        ggCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGc                                                                                                 <  1:361816/134‑1 (MQ=255)                                         gCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCa                                                                                          <  1:584383/140‑1 (MQ=255)                                             tAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGc                                                                                                 >  1:1141194/1‑129 (MQ=255)                                             tAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGc                                                                                                 <  2:1141194/129‑1 (MQ=255)                                                  ttGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGtct                                                                                 >  2:312741/1‑140 (MQ=255)                                                  ttGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGtct                                                                                 <  2:903583/140‑1 (MQ=255)                                                   tGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGtctc                                                                                >  2:393396/1‑140 (MQ=255)                                                          cACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGcc                                                                           >  2:1258167/1‑138 (MQ=255)                                                          cACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGcc                                                                           <  1:1258167/138‑1 (MQ=255)                                                             gTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTg                                                                      <  1:589167/140‑1 (MQ=255)                                                               tAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCa                                                                    >  1:153799/1‑140 (MQ=255)                                                                  tctctcCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAgg                                                                 >  2:652661/1‑140 (MQ=255)                                                                  tctctcCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTCTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAgg                                                                 >  1:848003/1‑140 (MQ=255)                                                                    tctcCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCa                                                               <  1:652661/140‑1 (MQ=255)                                                                       ccGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATa                                                            <  2:565614/140‑1 (MQ=255)                                                                       ccGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATa                                                            <  2:153799/140‑1 (MQ=255)                                                                        cGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATaa                                                           >  1:819800/1‑140 (MQ=255)                                                                         gtgtTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATaaa                                                          <  2:1061997/140‑1 (MQ=255)                                                                         gtgtTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATaaa                                                          <  2:819800/140‑1 (MQ=255)                                                                         gtgtTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATaaa                                                          <  2:271732/140‑1 (MQ=255)                                                                           gtTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATaaa                                                          >  2:459671/1‑138 (MQ=255)                                                                           gtTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATaaa                                                          <  1:459671/138‑1 (MQ=255)                                                                           gtTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAAcc                                                        >  1:305430/1‑140 (MQ=255)                                                                           gtTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAAcc                                                        >  2:145430/1‑140 (MQ=255)                                                                            ttAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCaa                                                                   >  1:1300624/1‑128 (MQ=255)                                                                            ttAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCaa                                                                   <  2:1300624/128‑1 (MQ=255)                                                                                ttGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATa                                                   >  1:37119/1‑140 (MQ=255)                                                                                  gACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAac                                                 >  1:1045406/1‑140 (MQ=255)                                                                                   aCTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAaca                                                <  1:393396/140‑1 (MQ=255)                                                                                    cTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAacac                                               <  2:16136/140‑1 (MQ=255)                                                                                    cTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAacac                                               <  2:37119/140‑1 (MQ=255)                                                                                    cTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAacac                                               >  1:1290247/1‑140 (MQ=255)                                                                                      tGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACAt                                             >  1:820635/1‑140 (MQ=255)                                                                                        cccAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATcc                                           >  1:856230/1‑140 (MQ=255)                                                                                          cAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCca                                         <  2:726506/140‑1 (MQ=255)                                                                                          cAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTCTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCca                                         <  2:944623/140‑1 (MQ=255)                                                                                            aaaTTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCcaca                                       <  2:50889/140‑1 (MQ=255)                                                                                             aaTTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACaa                                      >  1:58548/1‑140 (MQ=255)                                                                                              aTTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAAc                                     <  2:305430/140‑1 (MQ=255)                                                                                              aTTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAAc                                     <  1:1087604/140‑1 (MQ=255)                                                                                                  aGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGa                                 >  1:1204093/1‑140 (MQ=255)                                                                                                  aGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGa                                 <  2:700245/140‑1 (MQ=255)                                                                                                         ctGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATcc                                           <  2:1274976/123‑1 (MQ=255)                                                                                                         ctGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATcc                                           >  1:1274976/1‑123 (MQ=255)                                                                                                         ctGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATaa                          >  2:462889/1‑140 (MQ=255)                                                                                                         ctGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATaa                          >  1:589759/1‑140 (MQ=255)                                                                                                              ggCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATgcgc                     >  1:558004/1‑140 (MQ=255)                                                                                                                    cTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGAt                                >  2:466272/1‑123 (MQ=255)                                                                                                                    cTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGAt                                <  1:466272/123‑1 (MQ=255)                                                                                                                              ctGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATgcgc                     >  2:1044030/1‑124 (MQ=255)                                                                                                                              ctGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATgcgc                     <  1:1044030/124‑1 (MQ=255)                                                                                                                                 ttttATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCa  <  2:558004/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    CTGCGCTGCCATCCCGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCA  >  NC_000913/3068021‑3068288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑