Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,164,769	G→A	A402V (GCG→GTG)	parC ←	DNA topoisomerase IV subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,164,769	0	G	A	100.0%	185.4 / NA	53	A402V (GCG→GTG)	parC	DNA topoisomerase IV subunit A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/28);  total (25/28)

GCAACTGGTCGCGCTCTTTTTCCAGTTCACTCTGCTCACCGCGAATCTTCATCTCTTCCAGTTTGGCAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCGCCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGAGGATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCGCACGG  >  NC_000913/3164632‑3164904                                                                                                                                          |                                                                                                                                        gCAACTGGTCGCGCTCTTTTTCCAGTTCACTCTGCTCACCGCGAATCTTCATCTCTTCCAGTTTGGCAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCAcc                                                                                                                                       >  2:1340354/1‑140 (MQ=255)  cAACTGGTCGCGCTCTTTTTCCAGTTCACTCTGCTCACCGCGAATCTTCATCTCTTCCAGTTTGGCAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCg                                                                                                                                      >  2:60165/1‑140 (MQ=255)       ggTCGCGCTCTTTTTCCAGTTCACTCTGCTCACCGCGAATCTTCATCTCTTCCAGTTTGGCAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTc                                                                                                                                 >  1:643322/1‑140 (MQ=255)        gTCGCGCTCTTTTTCCAGTTCACTCTGCTCACCGCGAATCTTCATCTCTTCCAGTTTGGCAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCg                                                                                                                                >  1:1112347/1‑140 (MQ=255)            cgcTCTTTTTCCAGTTCACTCTGCTCACCGCGAATCTTCATCTCTTCCAGTTTGGCAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGt                                                                                                                              >  2:1046190/1‑138 (MQ=255)            cgcTCTTTTTCCAGTTCACTCTGCTCACCGCGAATCTTCATCTCTTCCAGTTTGGCAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGt                                                                                                                              <  1:1046190/138‑1 (MQ=255)            cgcTCTTTTTCCAGTTCACTCTGCTCACCGCGAATCTTCATCTCTTCCAGTTTGGCAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTc                                                                                                                            >  1:1089414/1‑140 (MQ=255)                 tttttCCAGTTCACTCTGCTCACCGCGAATCTTCATCTCTTCCAGTTTGGCAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCtt                                                                                                                       <  2:643322/140‑1 (MQ=255)                         gTTCACTCTGCTCACCGCGAATCTTCATCTCTTCCAGTTTGGCAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGa                                                                                                               <  1:784105/140‑1 (MQ=255)                          ttCACTCTGCTCACCGCGAATCTTCATCTCTTCCAGTTTGGCAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGaa                                                                                                              <  1:1061234/140‑1 (MQ=255)                           tCACTCTGCTCACCGCGAATCTTCATCTCTTCCAGTTTGGCAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAAt                                                                                                             >  1:1031627/1‑140 (MQ=255)                           tCACTCTGCTCACCGCGAATCTTCATCTCTTCCAGTTTGGCAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAAt                                                                                                             >  2:638469/1‑140 (MQ=255)                            cACTCTGCTCACCGCGAATCTTCATCTCTTCCAGTTTGGCAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATg                                                                                                            <  2:1112347/140‑1 (MQ=255)                                   cTCACCGCGAATCTTCATCTCTTCCAGTTTGGCAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGTCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCaa                                                                                                     >  2:480539/1‑140 (MQ=255)                                   cTCACCGCGAATCTTCATCTCTTCCAGTTTGGCAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCaa                                                                                                     >  1:500361/1‑140 (MQ=255)                                   cTCACCGCGAATCTTCATCTCTTCCAGTTTGGCAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCaa                                                                                                     >  1:1326175/1‑140 (MQ=255)                                   cTCACCGCGAATCTTCATCTCTTCCAGTTTGGCAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCaa                                                                                                     >  2:1344265/1‑140 (MQ=255)                                    tCACCGCGAATCTTCATCTCTTCCAGTTTGGCAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCAAt                                                                                                    <  1:464649/140‑1 (MQ=255)                                       ccGCGAATCTTCATCTCTTCCAGTTTGGCAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCAc                                                                                                 <  2:724928/140‑1 (MQ=255)                                        cgcgAATCTTCATCTCTTCCAGTTTGGCATGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACt                                                                                                >  1:182353/1‑140 (MQ=255)                                         gcgAATCTTCATCTCTTCCAGTTTGGCAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGAt                                                                                                          >  1:220935/1‑129 (MQ=255)                                         gcgAATCTTCATCTCTTCCAGTTTGGCAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGAt                                                                                                          <  2:220935/129‑1 (MQ=255)                                         gcgAATCTTCATCTCTTCCAGTTTGGCAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACtt                                                                                               >  1:972626/1‑140 (MQ=255)                                            aaTCTTCATCTCTTCCAGTTTGGCAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGTCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTtcgt                                                                                            <  1:480539/140‑1 (MQ=255)                                            aaTCTTCATCTCTTCCAGTTTGGCAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTtcgt                                                                                            <  2:883964/140‑1 (MQ=255)                                            aaTCTTCATCTCTTCCAGTTTGGCAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTtcgt                                                                                            <  2:1089414/140‑1 (MQ=255)                                              tCTTCATCTCTTCCAGTTTGGCAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTtcgtcg                                                                                          <  2:1044963/140‑1 (MQ=255)                                                  cATCTCTTCCAGTTTGGCAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGatat                                                                                      >  1:1105835/1‑140 (MQ=255)                                                       ctTCCAGTTTGGCAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGATATTgaga                                                                                 <  2:1326175/140‑1 (MQ=255)                                                         tCCAGTTTGGCAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGATATTGAGaaa                                                                               >  2:96172/1‑140 (MQ=255)                                                         tCCAGTTTGGCAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGATATTGAGaaa                                                                               >  2:980950/1‑140 (MQ=255)                                                         tCCAGTTTGGCAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGATATTGAGaaa                                                                               >  1:783889/1‑140 (MQ=255)                                                          ccAGTTTGGCAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAAc                                                                              >  2:482659/1‑140 (MQ=255)                                                             gTTTGGCAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGcc                                                                           <  2:777194/140‑1 (MQ=255)                                                             gTTTGGCAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGcc                                                                           <  1:638469/140‑1 (MQ=255)                                                                   cAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGCCACCAGc                                                                     <  2:768419/140‑1 (MQ=255)                                                                           cGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTc                                                             <  2:1031627/140‑1 (MQ=255)                                                                           cGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTc                                                             <  2:783889/140‑1 (MQ=255)                                                                           cGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTc                                                             <  1:60165/140‑1 (MQ=255)                                                                                    aGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGAGGATATg                                                    <  1:38281/140‑1 (MQ=255)                                                                                        cGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGAGGATATGCAgg                                                <  1:968733/140‑1 (MQ=255)                                                                                          aGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCg                                                            <  2:342557/126‑1 (MQ=255)                                                                                          aGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCg                                                            >  1:342557/1‑126 (MQ=255)                                                                                          aGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGAGGATATGCAGgcg                                              >  1:91254/1‑140 (MQ=255)                                                                                          aGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGAGGATATGCAGgcg                                              >  1:1315134/1‑140 (MQ=255)                                                                                           ggATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGAGGATATGCAGgcgc                                             <  1:482659/140‑1 (MQ=255)                                                                                                    ccGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGAGGATATGCAGGCGCTTGAGGACt                                    <  1:466090/140‑1 (MQ=255)                                                                                                      gCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGAGGATATGCAGGCGCTTGAGGACttt                                  <  1:96172/140‑1 (MQ=255)                                                                                                           ggTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGAGGATATGCAGGCGCTTGAGGACTTTCTCCa                             <  2:1315134/140‑1 (MQ=255)                                                                                                           ggTTTCCGTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGAGGATATGCAGGCGCTTGAGGACTTTCTCCa                             <  1:1004322/140‑1 (MQ=255)                                                                                                                  gTAAGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGAGGATATGCAGGCGCTTGAGGACTTTCTCCAGACGATa                      <  2:1105835/140‑1 (MQ=255)                                                                                                                    aaGGCCAAACCGCGACATCAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGAGGATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGt                    >  1:380181/1‑140 (MQ=255)                                                                                                                                      cAGCACCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGAGGATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCGCACgg  >  1:776576/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        GCAACTGGTCGCGCTCTTTTTCCAGTTCACTCTGCTCACCGCGAATCTTCATCTCTTCCAGTTTGGCAAGATGACGCAGTTTCAGTTCGAGGATCGCTTCCGCCTGGGTTTCCGTAAGGCCAAACCGCGACATCAGCGCCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGAGGATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCGCACGG  >  NC_000913/3164632‑3164904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑