Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,180,607	G→C	V63L (GTG→CTG)	ygiC →	putative enzyme with ATPase activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,180,607	0	G	C	100.0%	313.8 / NA	85	V63L (GTG→CTG)	ygiC	putative enzyme with ATPase activity
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (46/39);  total (46/39)

ACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCA  >  NC_000913/3180470‑3180742                                                                                                                                          |                                                                                                                                        aCGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAgtgctg                                                                                                                                       >  1:578258/1‑140 (MQ=255)                         ccATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAg                                                                                                               >  1:854678/1‑140 (MQ=255)                         ccATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAg                                                                                                               <  1:867352/140‑1 (MQ=255)                           aTGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCt                                                                                                             >  1:1245369/1‑140 (MQ=255)                            tGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCtg                                                                                                            >  1:331193/1‑140 (MQ=255)                            tGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCtg                                                                                                            >  2:1146189/1‑140 (MQ=255)                                  gCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGAcc                                                                                                      >  2:323413/1‑140 (MQ=255)                                    gAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCaa                                                                                                    >  1:877093/1‑140 (MQ=255)                                      gCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCt                                                                                                             >  1:1129209/1‑129 (MQ=255)                                      gCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCt                                                                                                             <  2:1129209/129‑1 (MQ=255)                                      gCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAAt                                                                                                  >  1:1133531/1‑140 (MQ=255)                                      gCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAAt                                                                                                  >  2:380089/1‑140 (MQ=255)                                      gCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAAt                                                                                                  >  1:297852/1‑140 (MQ=255)                                          tACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCg                                                                                              <  2:637823/140‑1 (MQ=255)                                          tACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCg                                                                                              <  2:1156006/140‑1 (MQ=255)                                           aCTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGc                                                                                             >  1:737511/1‑140 (MQ=255)                                                    aaGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCaaaa                                                                                    >  1:917151/1‑140 (MQ=255)                                                    aaGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCaaaa                                                                                    >  1:382144/1‑140 (MQ=255)                                                    aaGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCaaaa                                                                                    >  1:1013447/1‑140 (MQ=255)                                                     aGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAac                                                                                   <  2:1133531/140‑1 (MQ=255)                                                     aGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAac                                                                                   <  2:297852/140‑1 (MQ=255)                                                         gCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGGAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTGCGCATTCCAAAacacac                                                                               >  2:1215044/1‑140 (MQ=255)                                                         gCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCg                                                                                              <  1:801290/125‑1 (MQ=255)                                                         gCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCg                                                                                              >  2:801290/1‑125 (MQ=255)                                                         gCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAacacac                                                                               >  1:142034/1‑140 (MQ=255)                                                         gCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAacacac                                                                               <  2:382144/140‑1 (MQ=255)                                                         gCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAacacac                                                                               >  2:649483/1‑140 (MQ=255)                                                         gCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAacacac                                                                               >  1:705263/1‑140 (MQ=255)                                                          cTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACAcc                                                                              >  2:188609/1‑140 (MQ=255)                                                            tactacAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTg                                                                            >  2:731073/1‑140 (MQ=255)                                                                   aGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGtttt                                                                     >  2:992716/1‑140 (MQ=255)                                                                    gTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTtg                                                                    >  1:1028872/1‑140 (MQ=255)                                                                    gTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTtg                                                                    >  1:149926/1‑140 (MQ=255)                                                                       gACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTgc                                                                 >  1:669907/1‑140 (MQ=255)                                                                        aCCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTgcg                                                                <  2:1338614/140‑1 (MQ=255)                                                                        aCCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTgcg                                                                <  2:331193/140‑1 (MQ=255)                                                                        aCCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTgcg                                                                <  2:1245369/140‑1 (MQ=255)                                                                        aCCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTgcg                                                                <  1:380089/140‑1 (MQ=255)                                                                        aCCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTgcg                                                                >  1:58201/1‑140 (MQ=255)                                                                         cccTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTgcgc                                                               >  1:1277640/1‑140 (MQ=255)                                                                         cccTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTgcgc                                                               >  2:1092116/1‑140 (MQ=255)                                                                             cGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGt                                                           >  2:492039/1‑140 (MQ=255)                                                                              gCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTc                                                          <  2:333880/140‑1 (MQ=255)                                                                               cccAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTgcg                                                                <  2:431573/133‑1 (MQ=255)                                                                               cccAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTgcg                                                                >  1:431573/1‑133 (MQ=255)                                                                               cccAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCa                                                         >  1:325462/1‑140 (MQ=255)                                                                                ccAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCAt                                                        <  1:649483/140‑1 (MQ=255)                                                                                  aGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCAt                                                        >  1:435217/1‑138 (MQ=255)                                                                                  aGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCAt                                                        <  2:435217/138‑1 (MQ=255)                                                                                  aGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATgg                                                      >  2:1147122/1‑140 (MQ=255)                                                                                    gTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCt                                                    >  1:40445/1‑140 (MQ=255)                                                                                     ttGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTg                                                   <  2:854678/140‑1 (MQ=255)                                                                                      tGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGa                                                  <  1:731073/140‑1 (MQ=255)                                                                                             cTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCa                                           >  1:1205527/1‑140 (MQ=255)                                                                                              tGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAg                                          >  1:721834/1‑140 (MQ=255)                                                                                               ggAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGc                                         >  2:532779/1‑140 (MQ=255)                                                                                                          ccgccgAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCAt                                      <  2:718352/132‑1 (MQ=255)                                                                                                          ccgccgAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCAt                                      <  2:1090903/132‑1 (MQ=255)                                                                                                          ccgccgAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCAt                                      >  1:1090903/1‑132 (MQ=255)                                                                                                          ccgccgAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCAt                                      >  1:718352/1‑132 (MQ=255)                                                                                                           cgccgAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCAt                                      >  2:810888/1‑131 (MQ=255)                                                                                                           cgccgAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCAt                                      <  1:810888/131‑1 (MQ=255)                                                                                                           cgccgAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTAtt                             >  2:199948/1‑140 (MQ=255)                                                                                                           cgccgAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTAtt                             <  1:492039/140‑1 (MQ=255)                                                                                                            gccgAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTc                            <  2:737511/140‑1 (MQ=255)                                                                                                            gccgAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTc                            <  2:325462/140‑1 (MQ=255)                                                                                                             ccgAACTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTcg                           <  2:142034/140‑1 (MQ=255)                                                                                                                 aCTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTc                       <  1:65898/140‑1 (MQ=255)                                                                                                                 aCTGCACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTc                       <  2:149926/140‑1 (MQ=255)                                                                                                                     cACCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGa                   >  1:158730/1‑140 (MQ=255)                                                                                                                      aCCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGAt                  <  2:1222774/140‑1 (MQ=255)                                                                                                                      aCCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGAt                  <  2:721834/140‑1 (MQ=255)                                                                                                                      aCCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGAt                  <  1:323413/140‑1 (MQ=255)                                                                                                                      aCCAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGAt                  <  1:1146189/140‑1 (MQ=255)                                                                                                                       ccAGATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATc                 >  1:454837/1‑140 (MQ=255)                                                                                                                          gATGTGCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTgg              <  2:594345/140‑1 (MQ=255)                                                                                                                            tgtgCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGATGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCg            <  2:1205527/140‑1 (MQ=255)                                                                                                                            tgtgCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCg            <  1:1215044/140‑1 (MQ=255)                                                                                                                            tgtgCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCg            <  1:1092116/140‑1 (MQ=255)                                                                                                                               gCCTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTgg         >  1:274478/1‑140 (MQ=255)                                                                                                                                 cTGAAAGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGa       >  1:331825/1‑140 (MQ=255)                                                                                                                                    aaaGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATgg    <  2:1277640/140‑1 (MQ=255)                                                                                                                                    aaaGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATgg    <  1:992716/140‑1 (MQ=255)                                                                                                                                     aaGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCg            >  2:5788/1‑131 (MQ=255)                                                                                                                                     aaGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCg            <  1:5788/131‑1 (MQ=255)                                                                                                                                      aGTGCTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCa  <  1:532779/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        ACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCA  >  NC_000913/3180470‑3180742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑