Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,213,348 A→G Y101C (TAC→TGC)  rpoD → RNA polymerase, sigma 70 (sigma D) factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,213,3480AG100.0% 385.2 / NA 105Y101C (TAC→TGC) rpoDRNA polymerase, sigma 70 (sigma D) factor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (47/58);  total (47/58)

Only 100 of 106 total aligned reads displayed.

GATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGG  >  NC_000913/3213211‑3213482
                                                                                                                                         |                                                                                                                                      
gATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGACGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCa                                                                                                                                      <  2:642041/140‑1 (MQ=255)
 aTGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCAt                                                                                                                                     >  1:469327/1‑140 (MQ=255)
 aTGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCAt                                                                                                                                     >  1:1194998/1‑140 (MQ=255)
   ggAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGc                                                                                                                                   >  1:756102/1‑140 (MQ=255)
   ggAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGc                                                                                                                                   >  1:133935/1‑140 (MQ=255)
        aagCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGaa                                                                                                                              >  2:723586/1‑140 (MQ=255)
         agCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGaaa                                                                                                                             >  1:604918/1‑140 (MQ=255)
           cACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATg                                                                                                                           >  2:211838/1‑140 (MQ=255)
           cACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATg                                                                                                                           >  2:1048893/1‑140 (MQ=255)
           cACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATg                                                                                                                           >  2:179101/1‑140 (MQ=255)
            aCCGGATGCCGATGATCTGATGCTGGCTGAAAACACCG‑GGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATggg                                                                                                                         <  1:849327/140‑1 (MQ=255)
             ccGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATggg                                                                                                                         <  1:1115754/140‑1 (MQ=255)
             ccGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATggg                                                                                                                         <  2:384717/140‑1 (MQ=255)
             ccGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATggg                                                                                                                         <  1:1339102/140‑1 (MQ=255)
             ccGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATggg                                                                                                                         <  2:1212648/140‑1 (MQ=255)
             ccGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATggg                                                                                                                         <  2:648486/140‑1 (MQ=255)
             ccGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATggg                                                                                                                         <  2:766870/140‑1 (MQ=255)
               ggATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATggg                                                                                                                         >  2:136338/1‑138 (MQ=255)
               ggATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATggg                                                                                                                         <  1:136338/138‑1 (MQ=255)
               ggATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCa                                                                                                                       >  2:389463/1‑140 (MQ=255)
               ggATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCa                                                                                                                       >  2:279799/1‑140 (MQ=255)
                   gCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGt                                                                                                                   >  1:54838/1‑140 (MQ=255)
                   gCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGt                                                                                                                   >  2:759706/1‑140 (MQ=255)
                    ccGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGtt                                                                                                                  >  1:411681/1‑140 (MQ=255)
                     cggtgatCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTg                                                                                                                 <  1:95798/137‑1 (MQ=255)
                     cGATGATCTGCTGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTg                                                                                                                 <  1:934296/140‑1 (MQ=255)
                     cGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATggg                                                                                                                         >  2:1039917/1‑132 (MQ=255)
                     cGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATggg                                                                                                                         <  1:1039917/132‑1 (MQ=255)
                     cGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTg                                                                                                                 <  1:1362643/140‑1 (MQ=255)
                     cGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTg                                                                                                                 <  2:798452/140‑1 (MQ=255)
                                     gctgAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCcgcg                                                                                                  >  2:433551/1‑139 (MQ=255)
                                     gctgAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGa                                                                                                 >  2:300228/1‑140 (MQ=255)
                                     gctgAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGa                                                                                                 >  2:340052/1‑140 (MQ=255)
                                     gctgAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGa                                                                                                 <  2:469327/140‑1 (MQ=255)
                                     gctgAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGa                                                                                                 >  2:695276/1‑140 (MQ=255)
                                     gctgAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGa                                                                                                 >  2:79217/1‑140 (MQ=255)
                                     gctgAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGa                                                                                                 >  2:905752/1‑140 (MQ=255)
                                     gctgAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGa                                                                                                 >  1:1079781/1‑140 (MQ=255)
                                      ctgAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGaa                                                                                                >  2:122715/1‑140 (MQ=255)
                                       tgAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAg                                                                                               >  2:268477/1‑140 (MQ=255)
                                               ccGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAAtt                                                                                       >  2:72010/1‑140 (MQ=255)
                                               ccGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAAtt                                                                                       >  1:409355/1‑140 (MQ=255)
                                               ccGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGAACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAAtt                                                                                       >  2:1240440/1‑140 (MQ=255)
                                                 gcgGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGa                                                                                     >  2:1351995/1‑140 (MQ=255)
                                                 gcgGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGa                                                                                     <  2:409355/140‑1 (MQ=255)
                                                  cgGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGAc                                                                                    <  1:215326/140‑1 (MQ=255)
                                                        aaGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCt                                                                              <  1:179101/140‑1 (MQ=255)
                                                        aaGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCt                                                                              <  1:1139752/140‑1 (MQ=255)
                                                        aaGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCt                                                                              <  1:72010/140‑1 (MQ=255)
                                                        aaGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCt                                                                              <  1:211838/140‑1 (MQ=255)
                                                             gctgcCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTa                                                                             >  1:876067/1‑136 (MQ=255)
                                                             gctgcCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTa                                                                             <  2:876067/136‑1 (MQ=255)
                                                                 ccGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTAtt                                                                     <  1:1310839/140‑1 (MQ=255)
                                                                      gccgccGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGa                                                                <  2:411681/140‑1 (MQ=255)
                                                                      gccgccGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGa                                                                <  2:613130/140‑1 (MQ=255)
                                                                           cgcgCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGa                                                           <  1:389463/140‑1 (MQ=255)
                                                                               cAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTa                                                                       <  1:1128112/124‑1 (MQ=255)
                                                                               cAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTa                                                                       >  2:1128112/1‑124 (MQ=255)
                                                                                 ggTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAAcc                                                     <  1:1271404/140‑1 (MQ=255)
                                                                                 ggTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAAcc                                                     <  2:887644/140‑1 (MQ=255)
                                                                                 ggTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAAcc                                                     <  2:1194998/140‑1 (MQ=255)
                                                                                 ggTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAAcc                                                     <  2:769027/140‑1 (MQ=255)
                                                                                 ggTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAAcc                                                     <  1:759706/140‑1 (MQ=255)
                                                                                            gCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGc                                          >  1:465103/1‑140 (MQ=255)
                                                                                              gTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTc                                        <  1:340052/140‑1 (MQ=255)
                                                                                                 gAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGt                                     <  2:465103/140‑1 (MQ=255)
                                                                                                 gAATCTGAAATCGGACGCAAGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGt                                     >  2:498893/1‑140 (MQ=255)
                                                                                                  aaTCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCt                                         >  2:613453/1‑135 (MQ=255)
                                                                                                  aaTCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCt                                         <  1:613453/135‑1 (MQ=255)
                                                                                                  aaTCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGtt                                    >  2:1231645/1‑140 (MQ=255)
                                                                                                  aaTCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGtt                                    <  1:723586/140‑1 (MQ=255)
                                                                                                  aaTCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGtt                                    <  1:695276/140‑1 (MQ=255)
                                                                                                      tGAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTg                                >  1:1013985/1‑140 (MQ=255)
                                                                                                       gAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGa                               <  1:15442/140‑1 (MQ=255)
                                                                                                       gAAATCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGa                               <  1:122715/140‑1 (MQ=255)
                                                                                                        aaaTCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGaa                              <  1:79217/140‑1 (MQ=255)
                                                                                                         aaTCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAat                             >  1:1085227/1‑140 (MQ=255)
                                                                                                         aaTCGGGCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAat                             >  1:1222056/1‑140 (MQ=255)
                                                                                                              ggCGCACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCg                        >  2:345449/1‑140 (MQ=255)
                                                                                                               gcgcACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCgg                       <  1:345449/140‑1 (MQ=255)
                                                                                                                cgcACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGa                               >  1:700646/1‑131 (MQ=255)
                                                                                                                cgcACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGa                               <  2:700646/131‑1 (MQ=255)
                                                                                                                 gcACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGaa                     <  1:301642/140‑1 (MQ=255)
                                                                                                                  cACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAg                    >  2:538217/1‑140 (MQ=255)
                                                                                                                  cACGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAg                    >  1:120228/1‑140 (MQ=255)
                                                                                                                    cGACTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCg                  <  1:149901/140‑1 (MQ=255)
                                                                                                                      aCTGACCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGAt                <  1:268477/140‑1 (MQ=255)
                                                                                                                          aCCCGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCAcc            >  1:334186/1‑140 (MQ=255)
                                                                                                                            ccGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTa          <  1:538217/140‑1 (MQ=255)
                                                                                                                            ccGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTa          <  1:259634/140‑1 (MQ=255)
                                                                                                                            ccGGTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTa          <  1:279799/140‑1 (MQ=255)
                                                                                                                               gTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATct       >  2:257276/1‑140 (MQ=255)
                                                                                                                               gTACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATct       >  1:474352/1‑140 (MQ=255)
                                                                                                                                tACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTa          <  1:932081/136‑1 (MQ=255)
                                                                                                                                tACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATctg      <  1:116488/140‑1 (MQ=255)
                                                                                                                                tACGCATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATctg      <  1:1231645/140‑1 (MQ=255)
                                                                                                                                    cATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTgg  <  2:1049036/140‑1 (MQ=255)
                                                                                                                                    cATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTgg  <  2:1079781/140‑1 (MQ=255)
                                                                                                                                    cATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTgg  <  2:1085227/140‑1 (MQ=255)
                                                                                                                                    cATGTGCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTgg  >  2:124058/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                      
GATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGG  >  NC_000913/3213211‑3213482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑