Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,234,758 T→C K373R (AAG→AGG)  rlmG ← 23S rRNA m(2)G1835 methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,234,7580TC98.2% 197.6 / ‑4.5 56K373R (AAG→AGG) rlmG23S rRNA m(2)G1835 methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (21/34);  total (21/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTGGCAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCG  >  NC_000913/3234622‑3234894
                                                                                                                                        |                                                                                                                                        
gAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCa                                                                                                                                       >  2:1172026/1‑140 (MQ=255)
gAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCa                                                                                                                                       <  1:80241/140‑1 (MQ=255)
gAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCa                                                                                                                                       <  2:148053/140‑1 (MQ=255)
  aCTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCAcc                                                                                                                                     <  1:1323054/140‑1 (MQ=255)
      ggCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTGTCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCAcc                                                                                                                                     <  2:300279/136‑1 (MQ=255)
      ggCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTGTCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCAcc                                                                                                                                     >  1:300279/1‑136 (MQ=255)
      ggCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCtt                                                                                                                                 >  2:89925/1‑140 (MQ=255)
      ggCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCtt                                                                                                                                 >  2:629720/1‑140 (MQ=255)
      ggCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCt                                                                                                                                  >  2:420366/1‑139 (MQ=255)
      ggCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCt                                                                                                                                  <  1:420366/139‑1 (MQ=255)
        cAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCttt                                                                                                                                >  1:25371/1‑139 (MQ=255)
        cAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCttt                                                                                                                                <  2:25371/139‑1 (MQ=255)
           aCATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGCCCCAGCCTCACCGCTTTCAGt                                                                                                                            >  1:458034/1‑140 (MQ=255)
               tGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACca                                                                                                                        <  2:310179/140‑1 (MQ=255)
                          aCGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACCACAAATttatta                                                                                                             <  1:1363330/140‑1 (MQ=255)
                                    cgTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCaa                                                                                                   <  1:89925/140‑1 (MQ=255)
                                    cgTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCaa                                                                                                   <  1:548291/140‑1 (MQ=255)
                                          aTCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAgtgg                                                                                             >  1:1240195/1‑140 (MQ=255)
                                          aTCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCACTTTCAGTACCACAAATTTATTATTCGTCGCAATAgtgg                                                                                             >  2:577824/1‑140 (MQ=255)
                                                ccTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGt                                                                                       >  1:497222/1‑140 (MQ=255)
                                                   ggAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGc                                                                                    <  2:43218/140‑1 (MQ=255)
                                                     aGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCg                                                                                  <  1:1301194/140‑1 (MQ=255)
                                                      gTAACGTGCTGAACGTCGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGa                                                                                 <  2:1224573/140‑1 (MQ=255)
                                                         aCGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGaaaa                                                                              >  2:454741/1‑140 (MQ=255)
                                                          cGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAAt                                                                             >  1:271291/1‑140 (MQ=255)
                                                              cTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAAtcttc                                                                         >  1:412950/1‑140 (MQ=255)
                                                              cTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAAtcttc                                                                         >  2:568762/1‑140 (MQ=255)
                                                               tGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAAtcttct                                                                        >  1:319940/1‑140 (MQ=255)
                                                                gAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATcttctt                                                                       <  2:666364/140‑1 (MQ=255)
                                                                gAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCACTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATcttctt                                                                       <  1:577824/140‑1 (MQ=255)
                                                                  aCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCa                                                                     <  1:1172026/140‑1 (MQ=255)
                                                                      aGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGttt                                                                 >  1:1028461/1‑140 (MQ=255)
                                                                       ggCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTa                                                                <  2:205757/140‑1 (MQ=255)
                                                                        gCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGCCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTAt                                                               <  2:458034/140‑1 (MQ=255)
                                                                           ggATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGa                                                            >  1:244948/1‑140 (MQ=255)
                                                                                 ggCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAAt                                                      >  1:378213/1‑140 (MQ=255)
                                                                                  gCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTAt                                                               <  1:39245/130‑1 (MQ=255)
                                                                                  gCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTAt                                                               >  2:39245/1‑130 (MQ=255)
                                                                                  gCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATc                                                     <  1:872081/140‑1 (MQ=255)
                                                                                       tACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGt                                                >  2:166084/1‑140 (MQ=255)
                                                                                               aTCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTg                                        >  1:7810/1‑140 (MQ=255)
                                                                                                tCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTgg                                       <  1:1208197/140‑1 (MQ=255)
                                                                                                tCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTgg                                       <  2:319940/140‑1 (MQ=255)
                                                                                                tCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTgg                                       <  1:412547/140‑1 (MQ=255)
                                                                                                tCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTgg                                       <  2:378213/140‑1 (MQ=255)
                                                                                                tCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTgg                                       <  1:568762/140‑1 (MQ=255)
                                                                                                tCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTgg                                       <  2:244948/140‑1 (MQ=255)
                                                                                                tCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTgg                                       <  2:1028461/140‑1 (MQ=255)
                                                                                                  cGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTGGCa                                     <  1:454741/140‑1 (MQ=255)
                                                                                                     cAGGTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTGGCAACg                                  <  1:166084/140‑1 (MQ=255)
                                                                                                        gTAACTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTGGCAACGata                               <  1:629720/140‑1 (MQ=255)
                                                                                                            cTCCCAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTGGCAACGATATACa                           <  2:271291/140‑1 (MQ=255)
                                                                                                             tCCCAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTGGCAACGATATACAg                          <  2:7810/140‑1 (MQ=255)
                                                                                                               ccAATCTTACCGACGACGTCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTGGCAACGATATACAGCt                        <  2:112634/140‑1 (MQ=255)
                                                                                                                                 tCCCAGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTGGCAACGATATACAGCTCGCCGTTGATTTTCAGGc      <  1:1358357/140‑1 (MQ=255)
                                                                                                                                     aGCCTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTGGCAACGATATACAGCTCGCCGTTGATTTTCAGGCAgcg  <  2:219555/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
GAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTGGCAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCG  >  NC_000913/3234622‑3234894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑