Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,253,814 A→G L134P (CTG→CCG)  yhaJ ← DNA‑binding transcriptional activator YhaJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,253,8140AG100.0% 162.4 / NA 45L134P (CTG→CCG) yhaJDNA‑binding transcriptional activator YhaJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/14);  total (31/14)

CGCGGCAACGTAGACGTTCATTAACGTATAGAGCTTGCGCGAGTTGATCTCCGACGAGGAACGAAAATGCATATCCGGCGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCAGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAACCGCGCGCGAGAGCTTC  >  NC_000913/3253684‑3253950
                                                                                                                                  |                                                                                                                                        
cgcgGCAACGTAGACGTTCATTAACGTATAGAGCTTGCGCGAGTTGATCTCCGACGAGGAACGAAAATGCATATCCGGCGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCtg                                                                                                                                 >  1:644785/1‑140 (MQ=255)
    gCAACGTAGACGTTCATTAACGTATAGAGCTTGCGCGAGTTGATCTCCGACGAGGAACGAAAATGCATATCCGGCGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGtgat                                                                                                                             >  1:787752/1‑140 (MQ=255)
        ggTAGACTTTCATAACCGTATAGAGCTGGCGCGATTTGATCCCCGACGAGAAAAAAAAAGCCATACCGGGCGCAAAAAAAATACCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGAtt                                                                                                                         <  2:838437/139‑1 (MQ=255)
                        cGTATAGAGCTTGCGCGAGTTGATCTCCGACGAGGAACGAAAATGCATATCCGGCGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTgg                                                                                                         >  1:436826/1‑140 (MQ=255)
                          tataGGGTTGGCGCGAGTTGCTCCCCGACGAGAAACAAAAATGAATCCCGGGCGCAAAAAAAATTCCCCCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCt                                                                                                       <  2:436826/140‑1 (MQ=255)
                            taGAGCTTGCGCGAGTTGATCTCCGACGAGGAACGAAAATGCATATCCGGCGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCttt                                                                                                     >  1:24605/1‑140 (MQ=255)
                             agagCTTGCGCGAGTTGATCTCCGACGAGGAACGAAAATGCATATCCGGCGCGATAACAATATCCGCCCGCCCCTGCTCCACCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCtttt                                                                                                    >  2:1194494/1‑140 (MQ=255)
                               agCTTGCGCGAGTTGATCTCCGACGAGGAACGAAAATGCATATCCGGTGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTgc                                                                                                  >  1:721573/1‑140 (MQ=255)
                               agCTTGCGCGAGTTGATCTCCGACGAGGAACGAAAATGCATATCCGGCGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTgc                                                                                                  >  1:779540/1‑140 (MQ=255)
                                 cTTGCGCGAGTTGATCTCCGACGAGGAACGAAAATGCATATCCGGCGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTgccg                                                                                                >  1:523785/1‑140 (MQ=255)
                                   tGCGCGAGTTGATCTCCGACGAGGAACGAAAATGCATATCCGGCGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTgccgcc                                                                                              >  1:581871/1‑140 (MQ=255)
                                   tGCGCGAGTTGATCTCCGACGAGGAACGAAAATGCATATCCGGCGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATCGGCTTTTgccgcc                                                                                              >  1:697150/1‑140 (MQ=255)
                                   tGCGCGAGTTGATCTCCGACGAGGAACGAAAATGCATATCCAGCGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTgccgcc                                                                                              >  2:669399/1‑140 (MQ=255)
                                     cgcgAGTTGATCTCCGACGAGGAACGAAAATGCATATCCGGCGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAg                                                                                            >  1:1085543/1‑140 (MQ=255)
                                              aTCTCCGACGAGAAACAAAAATGCATACCCGGCGCGATAACAATACCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGAt                                                                                   <  1:156925/140‑1 (MQ=255)
                                              aTCCCCGACGAGAAACAAAAATGCATTCCCGGCGGAAAAACAATACCCGCCCCCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGAt                                                                                   <  1:1225963/140‑1 (MQ=255)
                                                        aGGAACGAAAATGCATATCCGGCGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGaaa                                                                         >  2:1118080/1‑140 (MQ=255)
                                                        aGGAACGAAAATGCATATCCGGCGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGaaa                                                                         >  1:683246/1‑140 (MQ=255)
                                                           aaCGAAAATGCATATCCGGCGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAg                                                                      >  2:54589/1‑140 (MQ=255)
                                                                    gCATATCCGGCGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTc                                                             >  2:2100/1‑140 (MQ=255)
                                                                     cATATCCGGCGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCg                                                            >  1:789364/1‑140 (MQ=255)
                                                                     cATATCCGGCGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCC‑GTTTGTCGATTAACGGGAAAAAGGCAGGTGTCgg                                                           >  1:165299/1‑140 (MQ=255)
                                                                       tccccGGCGCAAAAAAAATACCCCCCCGCCCCTGCCCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGt                                                          <  2:581871/137‑1 (MQ=255)
                                                                              cgcgATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAgcg                                                   >  2:352972/1‑140 (MQ=255)
                                                                               gcgATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCTCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAgcgc                                                  >  2:268012/1‑140 (MQ=255)
                                                                                cgATAAAAATACCCCCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCt                                                 <  1:78108/140‑1 (MQ=255)
                                                                                aaaaaaaaaTACCCCCCCCCCCCTGCCCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCt                                                 <  2:1085543/133‑1 (MQ=255)
                                                                                             cgcccgccCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATgg                                    >  2:927189/1‑140 (MQ=255)
                                                                                               cccgcccCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTg                                  >  2:1286141/1‑140 (MQ=255)
                                                                                                ccgcccCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTga                                 >  1:149412/1‑140 (MQ=255)
                                                                                                 cgcccCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGCAAAATGCAGGAGTCGGTACCAGCGCTTCGGTCCCAATGGTgaa                                >  2:340880/1‑139 (MQ=255)
                                                                                                    cccTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATg                             <  1:1118080/140‑1 (MQ=255)
                                                                                                    cccTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATg                             <  2:721573/140‑1 (MQ=255)
                                                                                                      cTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCg                           <  1:352972/140‑1 (MQ=255)
                                                                                                       tGCCCCACCCTTCCCCCCCCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCGt                          <  1:1011273/140‑1 (MQ=255)
                                                                                                            cAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATCGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTccc                     <  2:697150/140‑1 (MQ=255)
                                                                                                              gCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCaa                   >  1:234558/1‑140 (MQ=255)
                                                                                                              gCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCaa                   >  2:296261/1‑140 (MQ=255)
                                                                                                              gCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAAC‑GGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAAc                  >  1:1240329/1‑140 (MQ=255)
                                                                                                                  ttCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAACcgc               >  1:1293980/1‑140 (MQ=255)
                                                                                                                   tCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAACcgcg              >  1:1144678/1‑140 (MQ=255)
                                                                                                                     ccACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAACcgcgcg            <  1:296261/140‑1 (MQ=255)
                                                                                                                       aCGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAACcgcgcgcg          >  1:356428/1‑140 (MQ=255)
                                                                                                                         gCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAACCGCGCGCgag        <  2:779540/140‑1 (MQ=255)
                                                                                                                         gCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAACCGCGCGCgag        >  2:1137405/1‑140 (MQ=255)
                                                                                                                              cGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCG‑CAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAACCGCGCGCGAGAGCTTc  >  2:376970/1‑140 (MQ=255)
                                                                                                                                  |                                                                                                                                        
CGCGGCAACGTAGACGTTCATTAACGTATAGAGCTTGCGCGAGTTGATCTCCGACGAGGAACGAAAATGCATATCCGGCGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCAGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAACCGCGCGCGAGAGCTTC  >  NC_000913/3253684‑3253950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑