Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,543,262	A→G	L32L (TTA→TTG)	rpnA →	recombination‑promoting nuclease RpnA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,543,262	0	A	G	100.0%	323.2 / NA	91	L32L (TTA→TTG)	rpnA	recombination‑promoting nuclease RpnA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/53);  total (38/53)

GCGTAGAGTCGCGGCATTCAAACAACAGGTGAAGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTACCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGAAAGCTATTCCGATGTGCTGTGGTCGGTGAAAACGGAACAAGGACCAGGATA  >  NC_000913/3543126‑3543393                                                                                                                                         |                                                                                                                                    gcgTAGAGTCGCGGCATTCAAACAACAGGTGAAGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCg                                                                                                                                  <  1:528644/140‑1 (MQ=255)  cgTAGAGTCGCGGCATTCAAACAACAGGTGAAGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCgg                                                                                                                                 <  1:550554/140‑1 (MQ=255)   gTAGAGTCGCGGCATTCAAACAACAGGTGAAGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGc                                                                                                                                <  2:532890/140‑1 (MQ=255)      gagTCGCGGCATTCAAACAACAGGTGAAGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCAcc                                                                                                                             <  1:1367156/140‑1 (MQ=255)         tCGCGGCATTCAAACAACAGGTGAAGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCAt                                                                                                                          >  2:526721/1‑140 (MQ=255)             ggCATTCAAACAACAGGTGAAGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCAc                                                                                                                      >  1:683249/1‑140 (MQ=255)             ggCATTCAAACAACAGGTGAAGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCAc                                                                                                                      >  1:595983/1‑140 (MQ=255)              gCATTCAAACAACAGGTGAAGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCAcg                                                                                                                     <  1:1212121/140‑1 (MQ=255)                 ttCAAACAACAGGGGAAGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACGCGCTGCGTTGTTCAAACTCTTTTTACGCCGACCGGACACGGCTCGTGATTTTCTTGCGATTCATTTGCCGGCACGCCTTCACgcgc                                                                                                                  >  2:1107574/1‑140 (MQ=255)                      acaacaGGTGAAGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTtgt                                                                                                             <  2:249168/140‑1 (MQ=255)                       caacaGGTGAAGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTtgtg                                                                                                            <  1:811312/140‑1 (MQ=255)                         acaGGTGAAGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGat                                                                                                          >  1:1088641/1‑140 (MQ=255)                          caGGTGAAGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGata                                                                                                         <  1:332/140‑1 (MQ=255)                           aGGTGAAGGAACGTCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCt                                                                                                                 >  2:350816/1‑131 (MQ=255)                           aGGTGAAGGAACGTCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCt                                                                                                                 <  1:350816/131‑1 (MQ=255)                            ggTGAAGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCTACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATg                                                                                                       >  1:1302205/1‑140 (MQ=255)                            ggTGAAGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATg                                                                                                       >  1:701605/1‑140 (MQ=255)                             gTGAAGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCt                                                                                                                 <  2:206672/129‑1 (MQ=255)                             gTGAAGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCt                                                                                                                 >  1:206672/1‑129 (MQ=255)                              tGAAGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGaa                                                                                                     <  2:1336743/140‑1 (MQ=255)                                aaGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCt                                                                                                                 <  1:86371/126‑1 (MQ=255)                                aaGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCt                                                                                                                 >  2:86371/1‑126 (MQ=255)                                aaGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGaaaa                                                                                                   <  2:595983/140‑1 (MQ=255)                                  ggAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAAcc                                                                                                 >  1:343015/1‑140 (MQ=255)                                    aaCGCCATGAGCAAAAAGCAGAGTTCCTCCCCACCCGATGCGCTGTTCAAACTCTGTTTCCGCCAACCGGACACGGCTCGTGATTTTCTTGCCTTTCATTTGCCGGCACCCATTCACGCCCTTTGTGATATGAAAACCCt                                                                                               <  2:1106218/140‑1 (MQ=255)                                    aaCGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCt                                                                                               <  2:343015/140‑1 (MQ=255)                                    aaCGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCt                                                                                               <  2:1280490/140‑1 (MQ=255)                                      cGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCa                                                                                             >  1:471451/1‑140 (MQ=255)                                       gCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCaa                                                                                            >  1:136214/1‑140 (MQ=255)                                         cATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGc                                                                                          <  2:301671/140‑1 (MQ=255)                                         cATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGc                                                                                          <  2:845673/140‑1 (MQ=255)                                           tGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTg                                                                                        >  2:586112/1‑140 (MQ=255)                                             aGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGaa                                                                                                     >  1:546706/1‑125 (MQ=255)                                             aGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGaa                                                                                                     <  2:546706/125‑1 (MQ=255)                                             aGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCt                                                                                               <  2:1202659/131‑1 (MQ=255)                                             aGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCt                                                                                               >  1:1202659/1‑131 (MQ=255)                                                   aaGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGag                                                                                <  1:586112/140‑1 (MQ=255)                                                   aaGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGag                                                                                <  2:1302205/140‑1 (MQ=255)                                                       agagTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACTCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGagcagc                                                                            >  2:1235787/1‑140 (MQ=255)                                                        gagTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCt                                                                           >  2:1078056/1‑140 (MQ=255)                                                           ttCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTa                                                                        >  1:712581/1‑140 (MQ=255)                                                            tCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTAt                                                                       <  2:709869/140‑1 (MQ=255)                                                            tCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTAt                                                                       <  2:40590/140‑1 (MQ=255)                                                                ccccACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATtgat                                                                   <  1:768110/140‑1 (MQ=255)                                                                    acacGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACg                                                               <  1:752174/140‑1 (MQ=255)                                                                     cacGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTAt                                                                       <  2:1149351/131‑1 (MQ=255)                                                                     cacGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTAt                                                                       >  1:1149351/1‑131 (MQ=255)                                                                     cacGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGa                                                              >  2:14323/1‑140 (MQ=255)                                                                       cGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATc                                                            <  1:182533/140‑1 (MQ=255)                                                                        gATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCt                                                           >  1:88635/1‑140 (MQ=255)                                                                          tGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGc                                                         >  2:287929/1‑140 (MQ=255)                                                                          tGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGc                                                         >  1:504355/1‑140 (MQ=255)                                                                           gcgcTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCg                                                        >  2:1002861/1‑140 (MQ=255)                                                                           gcgcTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCg                                                        >  2:859274/1‑140 (MQ=255)                                                                           gcgcTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCg                                                        >  1:1090700/1‑140 (MQ=255)                                                                            cgcTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGt                                                       <  2:471451/140‑1 (MQ=255)                                                                            cgcTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGt                                                       >  2:491571/1‑140 (MQ=255)                                                                            cgcTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGt                                                       <  2:541395/140‑1 (MQ=255)                                                                              cTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGa                                                     <  2:1277256/140‑1 (MQ=255)                                                                              cTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGa                                                     >  1:1047298/1‑140 (MQ=255)                                                                              cTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGa                                                     <  1:1163924/140‑1 (MQ=255)                                                                               tGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGaa                                                    <  2:1088641/140‑1 (MQ=255)                                                                               tGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGaa                                                    <  1:1078056/140‑1 (MQ=255)                                                                                 ttCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGAAAg                                                  <  2:1047298/140‑1 (MQ=255)                                                                                 ttCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGAAAg                                                  >  2:1356876/1‑140 (MQ=255)                                                                                 ttCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGAAAg                                                  >  2:1230441/1‑140 (MQ=255)                                                                                 ttCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGAAAg                                                  <  1:859274/140‑1 (MQ=255)                                                                                      aCTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGTCGATCTGCGTGAAAGCTAtt                                             <  1:1107574/140‑1 (MQ=255)                                                                                         ctTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGAAAGCTATTCCg                                          <  1:287929/140‑1 (MQ=255)                                                                                           ttttACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGAAAGCTATTCCGAt                                        >  2:1157113/1‑140 (MQ=255)                                                                                                    aaCCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGAAAGCTATTCCGATGTGCTGTgg                               <  1:526721/140‑1 (MQ=255)                                                                                                         gACACGGCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGAAAGCTATTCCGATGTGCTGTGGTCGGt                          >  2:912886/1‑140 (MQ=255)                                                                                                              ggCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGAAAGCTATTCCGATGTGCTGTGGTCGGTGaaaa                     <  1:193512/140‑1 (MQ=255)                                                                                                               gCTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGAAAGCTATTCCGATGTGCTGTGGTCGGTGAAAAc                    >  2:148864/1‑140 (MQ=255)                                                                                                                cTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGAAAGCTATTCCGATGTGCTGTGGTCGGTGAAAACg                   >  2:992001/1‑140 (MQ=255)                                                                                                                cTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGAAAGCTATTCCGATGTGCTGTGGTCGGTGAAAACg                   >  1:621296/1‑140 (MQ=255)                                                                                                                cTCGTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGAAAGCTATTCCGATGTGCTGTGGTCGGTGAAAACg                   <  1:992001/140‑1 (MQ=255)                                                                                                                   gTGATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGAAAGCTATTCCGATGTGCTGTGGTCGGTGAAAACGGaa                >  2:268077/1‑140 (MQ=255)                                                                                                                    tGATTTTCTTGCGTTTCTTTTGCCGGCACCCATTCCCGCGCCTTGTGATATGAAAACCCCCAAGCTCGAATCGAGCAGCTTTATTGATGACGATCTGCGTGCAAGCTATTCCGCTGTGCCGTGGTCGGTGAAAACGGaaa               >  2:767096/1‑139 (MQ=255)                                                                                                                     gATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGAAAGCTATTCCGATGTGCTGTGGTCGGTGAAAACGGAACa              <  2:578161/140‑1 (MQ=255)                                                                                                                     gATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGAAAGCTATTCCGATGTGCTGTGGTCGGTGAAAACGGAACa              <  2:683249/140‑1 (MQ=255)                                                                                                                     gATTTTCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGAAAGCTATTCCGATGTGCTGTGGTCGGTGAAAACGGAACa              <  2:136214/140‑1 (MQ=255)                                                                                                                         ttCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGTTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGAAAGCTATTCCGATGTGCTGTGGTCGGTGAAAACGGAACAAGGa          <  1:872950/140‑1 (MQ=255)                                                                                                                         ttCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGAAAGCTATTCCGATGTGCTGTGGTCGGTGAAAACGGAACAAGGa          <  2:701605/140‑1 (MQ=255)                                                                                                                         ttCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGAAAGCTATTCCGATGTGCTGTGGTCGGTGAAAACGGAACAAGGa          <  1:402748/140‑1 (MQ=255)                                                                                                                          tCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGAAAGCTATTCCGATGTGCTGTGGTCGGTGAAAACGGAACAAGGAc         <  1:274822/140‑1 (MQ=255)                                                                                                                          tCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGAAAGCTATTCCGATGTGCTGTGGTCGGTGAAAACGGAACAAGGAc         <  1:166680/140‑1 (MQ=255)                                                                                                                          tCTTGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGAAAGCTATTCCGATGTGCTGTGGTCGGTGAAAACGGAACAAGGAc         <  2:88635/140‑1 (MQ=255)                                                                                                                             tGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGAAAGCTATTCCGATGTGCTGTGGTCGGTGAAAACGGAACAAGGACCAg      <  1:622954/140‑1 (MQ=255)                                                                                                                             tGCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGAAAGCTATTCCGATGTGCTGTGGTCGGTGAAAACGGAACAAGGACCAg      <  1:14323/140‑1 (MQ=255)                                                                                                                              gCGTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGAAAGCTATTCCGATGTGCTGTGGTCGGTGAAAACGGAACAAGGACCAgg     <  2:621296/140‑1 (MQ=255)                                                                                                                                gTTTCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGAAAGCTATTCCGATGTGCTGTGGTCGGTGAAAACGGAACAAGGACCAGGAt   >  2:892338/1‑140 (MQ=255)                                                                                                                                 tttCATTTGCCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGAAAGCTATTCCGATGTGCTGTGGTCGGTGAAAACGGAACAAGGACCAGGATa  <  1:892338/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    GCGTAGAGTCGCGGCATTCAAACAACAGGTGAAGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACACGGCTCGTGATTTTCTTGCGTTTCATTTACCGGCACCCATTCACGCGCTTTGTGATATGAAAACCCTCAAGCTGGAGTCGAGCAGCTTTATTGATGACGATCTGCGTGAAAGCTATTCCGATGTGCTGTGGTCGGTGAAAACGGAACAAGGACCAGGATA  >  NC_000913/3543126‑3543393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑