Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	412,047	C→T	P145P (CCG→CCA)	araJ ←	putative transport protein AraJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	412,047	0	C	T	100.0%	148.1 / NA	42	P145P (CCG→CCA)	araJ	putative transport protein AraJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/3);  total (39/3)

CGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCCAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGATAACACGATCGCTCCGACGCCAAAAAATGCGCCATGCGGAAAGCCGGATA  >  NC_000913/411911‑412183                                                                                                                                         |                                                                                                                                         cgcgCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCCAGCTAAATTCCTGACTTAAATACGTTCCCAGTGGa                                                                                                                                       <  1:255912/140‑1 (MQ=255)    gcAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCCAGCTAAATTCCTGACTTAAATACGTTCCCAGTGGAATg                                                                                                                                    <  2:772270/140‑1 (MQ=255)                  gCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAGACAGCGATCAATAAAAAGGTGTAACGCCAGCTAAATTCCTGACTTAAATACGTTCCCAGTGGAATGCCCAGCAAATTGGc                                                                                                                      >  2:943241/1‑140 (MQ=255)                  gCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAATGCCAGCTAAATTCCTGACTTAAATACGTTCCCAGTGGAATGCCCAGCAAATTGGc                                                                                                                      >  1:138451/1‑140 (MQ=255)                  gCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCCAGCTAAATTCCTGACTTAAATACGTTCCCAGTGGAATGCCCAGCAAATTGGc                                                                                                                      >  2:41140/1‑140 (MQ=255)                  gCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCCAGCTAAATTCCTGACTTAAATACGTTCCCAGTGGAATGCCCAGCAAATTGGc                                                                                                                      >  1:1348089/1‑140 (MQ=255)                  gCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCCAGCTAAATTCCTGACTTAAATACGTTCCCAGTGGAATGCCCAGCAAATTGGc                                                                                                                      >  1:1007057/1‑140 (MQ=255)                                     gCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCCAGCTAAATTCCTGACTTAAATACGTTCCCAGTGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAAcc                                                                                                   >  1:313340/1‑140 (MQ=255)                                      cACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCCAGCTAAATTCCTGACTTAAATACGTTCCCAGTGGAATGCCCAGCAAATTGGCGa                                                                                                                    <  1:971971/122‑1 (MQ=255)                                      cACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCCAGCTAAATTCCTGACTTAAATACGTTCCCAGTGGAATGCCCAGCAAATTGGCGa                                                                                                                    >  2:971971/1‑122 (MQ=255)                                          cAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCCAGCTAAATTCCTGACTTAAATACGTTCCCAGTGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATccc                                                                                              >  2:501374/1‑140 (MQ=255)                                          cAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCCAGCTAAATTCCTGACTTAAATACGTTCCCAGTGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATccc                                                                                              >  2:458616/1‑140 (MQ=255)                                                     cGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCCAGCTAAATTCCTGACTTAAATACGTTCCCAGTGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCAcggcgg                                                                                   >  2:607121/1‑140 (MQ=255)                                                      gATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCCAGCTAAATTCCTGACTTAAATACGTTCCCAGTGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACTATCCCCGCCAcggcggc                                                                                  >  1:357949/1‑140 (MQ=255)                                                       aTGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCCAGCTAAATTCCTGACTTAAATACGTTCCCAGTGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCAcggcggcg                                                                                 >  2:1095509/1‑140 (MQ=255)                                                                aacagatatTAAAAACAGCAATAAAAAAAAGGGTGTAACGCCAGCTAATTTCCTGACTAAAATCCGTCCCCAGTGGAATCCCCAGAAATTTGGCGACTGTCACCCCGAAACCCACCCCCGCCACGGCGGCGGTGACtttt                                                                        <  2:2123/135‑1 (MQ=255)                                                                      tataAAAAACAGCGATAAATAAAAGGGTGAAACGCCAGCAAAATTCCTGACTTAAATACGTTCCCAGTGGAATCCCCAGCAAATGGGCGACTTTCACCCGGAAAACCACCCCCGCCACGGCGGCGGTGACTTTTCCGGGt                                                                  <  2:1007057/140‑1 (MQ=255)                                                                      tatTAAAAACAGCGATCAATAAAAAGGTGTAACGCCAGCTAAATTCCTGACTTAAATACGTTCCCAGTGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGt                                                                  >  2:549698/1‑140 (MQ=255)                                                                          aaaaaCAGCGATCAAAAAAAAGGTGTAACGCCAGCAAAATTCCTGACTAAAATACGTCCCCAGTGGAATGCCCAGCAAATTGGCGACTGTCACCCCGAAACCCACCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGa                                                              <  2:1073908/140‑1 (MQ=255)                                                                             aaCAGCGATCAATAAAAAGGTGTAACGCCAGCTAAATTCCTGACTTAAATACGTTCCCAGTGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATaa                                                           >  1:279256/1‑140 (MQ=255)                                                                                aGCGATCAATAAAAAGGTGTAACGCCAGCTAAATTCCTGACTTAAATACGTTCCCAGTGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGAtctgtc                                                        >  1:2123/1‑135 (MQ=255)                                                                                aGCGATCAATAAAAAGGTGTAACGCCAGCTAAATTCCTGACTTAAATACGTTCCCAGTGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAAttt                                                        >  2:173721/1‑140 (MQ=255)                                                                                aGCGATCAATAAAAAGGTGTAACGCCAGCTAAATTCCTGACTTAAATACGTTCCCAGTGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAAttt                                                        >  2:1323801/1‑140 (MQ=255)                                                                                aGCGATCAATAAAAAGGTGTAACGCCAGCTAAATTCCTGACTTAAATACGTTCCCAGTGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAAttt                                                        >  1:282136/1‑140 (MQ=255)                                                                                aGCGATCAATAAAAAGGTGTAACGCCAGCTAAATTCCTGACTTAAATACGTTCCCAGTGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAAttt                                                        >  2:918123/1‑140 (MQ=255)                                                                                 gCGATAAAAAAAAGGGTGAAACCCCAGCTAATTTCCTGACTAAAATACGTCCCCGGTGGAAGCCCCAGCAAATGGGCGACTGTCACCCCGAAAACCACCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAAtttt                                                       <  2:1001749/140‑1 (MQ=255)                                                                                  cGATCAATAAAAAGGTGTAACGCCAGCTAAATTCCTGACTTAAATACGTTCCCAGTGGAATGCCCAGCAAATTGGGGACTGTCATCCCGGAAACCATCCCCGCCACAGCGGCGGTGACTTTTCCGGGTTTGATAAttttt                                                      >  2:1088578/1‑140 (MQ=255)                                                                                  cGATCAATAAAAAGGTGTAACGCCAGCTAAATTCCTGACTTAAATACGTTCCCAGTGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAAttttt                                                      >  2:115761/1‑140 (MQ=255)                                                                                  cGATCAATAAAAAGGTGTAACGCCAGCTAAATTCCTGACTTAAATACGTTCCCAGTGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAAttttt                                                      >  2:455139/1‑140 (MQ=255)                                                                                  cGATCAATAAAAAGGTGTAACGCCAGCTAAATTCCTGACTTAAATACGTTCCCAGTGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAAttttt                                                      >  1:1073908/1‑140 (MQ=255)                                                                                     aaaaaaaaaaGGTGTAACGCCAGCTAAATCCCTGACTTAAATACGTCCCCAGTGGAATCCCCAGAAAATTGGCGACTGTCACCCCGAAACCCACCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGAt                                                   <  2:282136/135‑1 (MQ=255)                                                                                       aaTAAAAAGGTGTAACGCCAGCTAAATCCCTGACTTAAATACGTCCCCAGTGGAATGCCCAGCAAATTGGCGACTGTCACCCCGGAACCCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGATaa                                                 <  2:357496/140‑1 (MQ=255)                                                                                                 tgtAACGCCAGCTAAATTCCTGACTTAAATACGTTCCCAGTGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGATAACACGATCGCt                                       >  2:1188452/1‑140 (MQ=255)                                                                                                 tgtAACGCCAGCTAAATTCCTGACTTAAATACGTTCCCAGTGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGATAACACGATCGCt                                       >  2:117907/1‑140 (MQ=255)                                                                                                 tgtAACGCCAGCTAAATTCCTGACTTAAATACGTTCCCAGTGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGATAACACGATCGCt                                       >  2:852910/1‑140 (MQ=255)                                                                                                 tgtAACGCCAGCTAAATTCCTGACTTAAATACGTTCCCAGTGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGATAACACGATCGCt                                       >  2:29315/1‑140 (MQ=255)                                                                                                   tAACGCCAGCTAAATTCCTGACTTAAATACGTTCCCAGTGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGATAACACGATCGCTcc                                     >  2:359905/1‑140 (MQ=255)                                                                                                    aaCGCCAGCTAAATTCCTGACTAAAATCCGTCCCCAGTGGAATCCCCAGAAAATTGGCGACTGTCACCCCGAAACCCACCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGATAACACGATCGCTCCg                                    <  2:1348089/140‑1 (MQ=255)                                                                                                          aGCTAAATTCCTGACTTAAATACGTTCCCAGTGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGATAACACGATCGCTCCGACGCCa                              >  2:1251911/1‑140 (MQ=255)                                                                                                           gCTAAATTCCTGACTTAAATACGTTCCCAGTGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGATAACACGATCGCTCCGACGCCaa                             >  1:935044/1‑140 (MQ=255)                                                                                                           gCTAAATTCCTGACTTAAATACGTTCCCAGTGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGATAACACGATCGCTCCGACGCCaa                             >  1:779378/1‑140 (MQ=255)                                                                                                           gCTAAATTCCTGACTTAAATACGTTCCCAGTGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGATAACACGATCGCTCCGACGCCaa                             >  1:525357/1‑140 (MQ=255)                                                                                                             aaaaTTCCTGACTAAAATACGTCCCCAGTGGAAGGCCCAGCAAATGGGCGACTGTCACCCCGAAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGATAACACGATCGCTCCGACGCCaaaa                           <  2:779378/139‑1 (MQ=255)                                                                                                                     tGACTTAAATACGTTCCCAGTGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGATAACACGATCGCTCCGACGCCAAAAAATGCGcc                   >  1:870230/1‑140 (MQ=255)                                                                                                                     tGACTTAAATACGTTCCCAGTGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGATAACACGATCGCTCCGACGCCAAAAAATGCGcc                   >  2:889469/1‑140 (MQ=255)                                                                                                                         ttAAATACGTTCCCAGTGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGATAACACGATCGCTCCGACGCCAAAAAATGCGCCATGc               >  2:301563/1‑140 (MQ=255)                                                                                                                            aaTACGTTCCAAGTGAAACGCCCAGAAAATGGGCGATTGCCAGCCCGAAACCCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGATAACACGATCGCTCCGACGCCAAAAAATGCGCCATGCGGa            <  1:549698/140‑1 (MQ=255)                                                                                                                                   tCCCAGTGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGATAACACGATCGCTCCGACGCCAAAAAATGCGCCATGCGGAAAGCCgg     >  2:242128/1‑140 (MQ=255)                                                                                                                                   tCCCAGTGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGATAACACGATCGCTCCGACGCCAAAAAATGCGCCATGCGGAAAGCCgg     >  2:1261934/1‑140 (MQ=255)                                                                                                                                   tCCCAGTGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGATAACACGATCGCTCCGACGCCAAAAAATGCGCCATGCGGAAAGCCgg     >  1:501011/1‑140 (MQ=255)                                                                                                                                   tCCCAGTGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGATAACACGATCGCTCCGACGCCAAAAAATGCGCCATGCGGAAAGCCgg     >  2:985251/1‑140 (MQ=255)                                                                                                                                      cAGTGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCACCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGATAACACGATCGCTCCGACGCCAAAAAATGCGCCATGCGGAAAGCCGGATa  <  1:29315/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCCAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGATAACACGATCGCTCCGACGCCAAAAAATGCGCCATGCGGAAAGCCGGATA  >  NC_000913/411911‑412183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑