Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,833,576	T→C	D321G (GAC→GGC)	yicI ←	alpha‑D‑xyloside xylohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,833,576	0	T	C	100.0%	247.3 / NA	68	D321G (GAC→GGC)	yicI	alpha‑D‑xyloside xylohydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/36);  total (32/36)

TCTCTTGTAACTCTTTAAAGACGGGGGATTTTTGACCGATATAGGGGTTAATCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGTCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGTTCCGCCATACCATCGATAAAGCTGTTTACCGTCGCTTCGTCGTAGTTGGTGGTAAATGA  >  NC_000913/3833451‑3833712                                                                                                                              |                                                                                                                                         tctcTTGTAACTCTTTAAAGACGGGGGATTTTTGACCGATATAGGGGTTAATCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCg                                                                                                                            <  2:315967/140‑1 (MQ=255)   tctTGTAACTCTTTAAAGACGGGGGATTTTTGACCGATATAGGGGTTAATCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGca                                                                                                                          <  1:74827/140‑1 (MQ=255)    ctTGTAACTCTTTAAAGACGGGGGATTTTTGACCGATATAGGGGTTAATCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGcac                                                                                                                         <  1:1005130/140‑1 (MQ=255)     ttGTAACTCTTTAAAGACGGGGGATTTTTGACCGATATAGGGGTTAATCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGcacc                                                                                                                        >  1:365187/1‑140 (MQ=255)       gTAACTCTTTAAAGACGGGGGATTTTTGACCGATATAGGGGTTAATCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGcaccac                                                                                                                      >  1:1080493/1‑140 (MQ=255)          aCTCTTTAAAGACGGGGGATTTTTGACCGATATAGGGGTTAATCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTgg                                                                                                                   >  1:1007707/1‑140 (MQ=255)           ctctTTAAAGACGGGGGATTTTTGACCGATATAGGGGTTAATCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGa                                                                                                                  <  2:668877/140‑1 (MQ=255)           ctctTTAAAGACGGGGGATTTTTGACCGATATAGGGGTTAATCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGa                                                                                                                  >  2:611417/1‑140 (MQ=255)               ttAAAGACGGGGGATTTTTGACCGATATAGGGGTTAATCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGc                                                                                                              >  1:57307/1‑140 (MQ=255)                  aaGACGGGGGATTTTTGACCGATATAGGGGTTAATCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCTCCTCTGGAAGGCttt                                                                                                           <  2:57307/140‑1 (MQ=255)                      cGGGGGATTTTTGACCGATATAGGGGTTAATCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATc                                                                                                       <  2:1080493/140‑1 (MQ=255)                        ggggATTTTTGACCGATATAGGGGTTAATCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCa                                                                                                         <  2:233188/136‑1 (MQ=255)                        ggggATTTTTGACCGATATAGGGGTTAATCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCa                                                                                                         >  1:233188/1‑136 (MQ=255)                        ggggATTTTTGACCGATATAGGGGTTAATCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCa                                                                                                     >  1:417395/1‑140 (MQ=255)                           gATTTTTGACCGATATAGGGGTTAATCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGaa                                                                                                  <  2:1124425/140‑1 (MQ=255)                                  gACCGATATAGGGGTTAATCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCa                                                                                           <  2:1007707/140‑1 (MQ=255)                                   aCCGATATAGGGGTTAATCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCaa                                                                                          <  2:365187/140‑1 (MQ=255)                                    ccGATATAGGGGTTAATCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCaaa                                                                                         <  1:36105/140‑1 (MQ=255)                                      gATATAGGGGTTAATCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGt                                                                                       <  1:349893/140‑1 (MQ=255)                                                  aaTCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGTGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCa                                                                           >  1:105261/1‑140 (MQ=255)                                                  aaTCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCa                                                                                           >  1:296175/1‑124 (MQ=255)                                                  aaTCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCa                                                                                           <  1:111564/124‑1 (MQ=255)                                                  aaTCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCa                                                                                           <  2:296175/124‑1 (MQ=255)                                                  aaTCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCa                                                                                           >  2:111564/1‑124 (MQ=255)                                                  aaTCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGt                                                                                       <  2:571850/128‑1 (MQ=255)                                                  aaTCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGt                                                                                       >  1:571850/1‑128 (MQ=255)                                                  aaTCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCa                                                                           >  1:1150791/1‑140 (MQ=255)                                                  aaTCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCa                                                                           >  1:935423/1‑140 (MQ=255)                                                  aaTCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCa                                                                           >  1:756464/1‑140 (MQ=255)                                                  aaTCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCa                                                                           >  2:168512/1‑140 (MQ=255)                                                  aaTCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCa                                                                           >  1:1044826/1‑140 (MQ=255)                                                  aaTCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCa                                                                           >  1:1038845/1‑140 (MQ=255)                                                  aaTCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCa                                                                           >  1:54069/1‑140 (MQ=255)                                                  aaTCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCa                                                                           >  1:443143/1‑140 (MQ=255)                                                  aaTCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCa                                                                           >  1:412489/1‑140 (MQ=255)                                                  aaTCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCa                                                                           >  2:459815/1‑140 (MQ=255)                                                  aaTCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCa                                                                           >  1:1254144/1‑140 (MQ=255)                                                  aaTCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCa                                                                           >  1:1176687/1‑140 (MQ=255)                                                       aGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGc                                                                      >  2:331383/1‑140 (MQ=255)                                                             aGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTg                                                                <  2:756464/140‑1 (MQ=255)                                                               aTTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCg                                                              <  1:611417/140‑1 (MQ=255)                                                               aTTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCg                                                              >  2:657109/1‑140 (MQ=255)                                                                    cAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGTGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGTTCCg                                                         <  2:105261/140‑1 (MQ=255)                                                                     aGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGTTCCGc                                                        <  1:459815/140‑1 (MQ=255)                                                                      gTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGTTCCGcc                                                       <  2:1004830/140‑1 (MQ=255)                                                                         cTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGTTCCGCCATa                                                    >  1:375329/1‑140 (MQ=255)                                                                           ttCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGTTCCGCCATAcc                                                  <  2:1044826/140‑1 (MQ=255)                                                                           ttCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGTTCCGCCATAcc                                                  <  2:54069/140‑1 (MQ=255)                                                                           ttCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACCCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGTTCCGCCATAcc                                                  >  1:1243302/1‑140 (MQ=255)                                                                            tCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGTTCCGCCATACCa                                                 >  1:1252767/1‑140 (MQ=255)                                                                                tttCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGTGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGTTCCGCCATACCATCGa                                             <  1:506019/140‑1 (MQ=255)                                                                                  tCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGTTCCGCCATACCATCGATa                                           <  2:52357/140‑1 (MQ=255)                                                                                                  cccTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGTTCCGCCATACCATCGATAAAGCTGTTTACCGTCg                           <  2:842510/140‑1 (MQ=255)                                                                                                  cccTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGTTCCGCCATACCATCGATAAAGCTGTTTACCGTCg                           <  2:443143/140‑1 (MQ=255)                                                                                                   ccTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGTTCCGCCATACCATCGATAAAGCTGTTTACCGTCGc                          <  2:1038845/140‑1 (MQ=255)                                                                                                   ccTTCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGTTCCGCCATACCATCGATAAAGCTGTTTACCGTCGc                          <  1:512694/140‑1 (MQ=255)                                                                                                      tCCGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGTTCCGCCATACCATCGATAAAGCTGTTTACCGTCGCTtc                       >  1:704242/1‑140 (MQ=255)                                                                                                       ccGGGTCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGTTCCGCCATACCATCGATAAAGCTGTTTACCGTCGCTtcg                      >  1:183860/1‑140 (MQ=255)                                                                                                            tCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGTTCCGCCATACCATCGATAAAGCTGTTTACCGTCGCt                         <  2:1227377/132‑1 (MQ=255)                                                                                                            tCAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGTTCCGCCATACCATCGATAAAGCTGTTTACCGTCGCt                         >  1:1227377/1‑132 (MQ=255)                                                                                                             cAGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGTTCCGCCATACCATCGATAAAGCTGTTTACCGTCGCTTCGTCGTAg                <  1:657109/140‑1 (MQ=255)                                                                                                              aGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGTTCCGCCATACCATCGATAAAGCTGTTTACCGTCGCTTCGTCGTAGt               <  2:374781/140‑1 (MQ=255)                                                                                                              aGGGAAAGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGTTCCGCCATACCATCGATAAAGCTGTTTACCGTCGCTTCGTCGTAGt               >  1:174346/1‑140 (MQ=255)                                                                                                              aGGGAAAGTCAGCGGGCCCCACCCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGTTCCGCCATACCATCGATAAAGCTGTTTACCGTCGCTTCGTCGTAGt               <  2:1243302/140‑1 (MQ=255)                                                                                                                  aaaGTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGTTCCGCCATACCATCGATAAAGCTGTTTACCGTCGCTTCGTCGTAGTtggt           <  2:935423/140‑1 (MQ=255)                                                                                                                     gTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGTTCCGCCATACCATCGATAAAGCTGTTTACCGTCGCTTCGTCGTAGTTggtggt        <  2:412489/140‑1 (MQ=255)                                                                                                                     gTCAGCGGGCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGTTCCGCCATACCATCGATAAAGCTGTTTACCGTCGCTTCGTCGTAGTTggtggt        <  2:183860/140‑1 (MQ=255)                                                                                                                           gggCCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGTTCCGCCATACCATCGATAAAGCTGTTTACCGTCGCTTCGTCGTAGTTGGTGGTAAATGa  <  1:168512/140‑1 (MQ=255)                                                                                                                              |                                                                                                                                         TCTCTTGTAACTCTTTAAAGACGGGGGATTTTTGACCGATATAGGGGTTAATCCAGACGCAGATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGTCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGTTCCGCCATACCATCGATAAAGCTGTTTACCGTCGCTTCGTCGTAGTTGGTGGTAAATGA  >  NC_000913/3833451‑3833712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑