Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	422,637	A→G	E40E (GAA→GAG)	malZ →	maltodextrin glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	422,637	0	A	G	100.0%	257.6 / NA	71	E40E (GAA→GAG)	malZ	maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/31);  total (40/31)

CATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTG  >  NC_000913/422500‑422771                                                                                                                                          |                                                                                                                                       catcaCAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAgca                                                                                                                                      <  2:737166/140‑1 (MQ=255)    cacaGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGa                                                                                                                                   <  1:944740/140‑1 (MQ=255)         gggAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACg                                                                                                                              >  2:369126/1‑140 (MQ=255)         gggAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACg                                                                                                                              >  2:731995/1‑140 (MQ=255)         gggAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACg                                                                                                                              >  2:45429/1‑140 (MQ=255)             aTTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACgaaga                                                                                                                          <  1:731995/140‑1 (MQ=255)             aTTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACgaaga                                                                                                                          <  1:369126/140‑1 (MQ=255)             aTTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACgaaga                                                                                                                          <  1:360270/140‑1 (MQ=255)             aTTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACgaaga                                                                                                                          <  1:45429/140‑1 (MQ=255)                   atgTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTc                                                                                                                    >  2:172107/1‑140 (MQ=255)                   atgTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTc                                                                                                                    >  2:442623/1‑140 (MQ=255)                     gTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAg                                                                                                                  >  2:183157/1‑140 (MQ=255)                      ttAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGt                                                                                                                 >  2:563436/1‑140 (MQ=255)                        aaaTGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTAc                                                                                                               >  2:1084694/1‑140 (MQ=255)                        aaaTGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTAc                                                                                                               <  1:172107/140‑1 (MQ=255)                         aaTGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTAcc                                                                                                              <  1:1242635/140‑1 (MQ=255)                         aaTGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTAcc                                                                                                              <  1:1084694/140‑1 (MQ=255)                         aaTGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTAcc                                                                                                              <  1:1323403/140‑1 (MQ=255)                           tGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGa                                                                                                            >  1:1247662/1‑140 (MQ=255)                           tGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGa                                                                                                            <  2:962960/140‑1 (MQ=255)                               tGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCa                                                                                                        >  2:1069116/1‑140 (MQ=255)                                ggCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCAt                                                                                                       <  1:490588/140‑1 (MQ=255)                                ggCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCAt                                                                                                       >  2:1079085/1‑140 (MQ=255)                                ggCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCAt                                                                                                       >  1:1218315/1‑140 (MQ=255)                                     cTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAagca                                                                                                  >  1:142936/1‑140 (MQ=255)                                       gCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAagcagc                                                                                                >  1:1021176/1‑140 (MQ=255)                                       gCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAagcagc                                                                                                >  2:313472/1‑140 (MQ=255)                                            tGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAagca                                                                                                  <  2:1076740/133‑1 (MQ=255)                                            tGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAagca                                                                                                  >  1:1076740/1‑133 (MQ=255)                                             gCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTc                                                                                          <  1:1079085/140‑1 (MQ=255)                                                  cATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCg                                                                                     <  1:193239/140‑1 (MQ=255)                                                  cATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCg                                                                                     <  1:791710/140‑1 (MQ=255)                                                   aTTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAACAGCGCAGTCAGCCGc                                                                                    <  2:64398/140‑1 (MQ=255)                                                          aaaCAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCg                                                                                     <  2:1046580/132‑1 (MQ=255)                                                          aaaCAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCg                                                                                     >  1:1046580/1‑132 (MQ=255)                                                            aCAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCg                                                                           <  2:800833/140‑1 (MQ=255)                                                                agcaaagATCAACTGCTCATTACACTGTGGCTGATGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGcc                                                                                      >  1:1290119/1‑125 (MQ=255)                                                                agcaaagATCAACTGCTCATTACACTGTGGCTGATGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGcc                                                                                      <  2:1290119/125‑1 (MQ=255)                                                                agcaaagATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCAc                                                                       >  2:478300/1‑140 (MQ=255)                                                                          aaCTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGt                                                             >  1:409558/1‑140 (MQ=255)                                                                           aCTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTg                                                            <  1:442623/140‑1 (MQ=255)                                                                            cTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTgc                                                           >  2:549467/1‑140 (MQ=255)                                                                                         tgtgGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGAtctc                                              >  1:1244576/1‑140 (MQ=255)                                                                                         tgtgGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGAtctc                                              >  2:588470/1‑140 (MQ=255)                                                                                         tgtgGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGAtctc                                              >  2:183107/1‑140 (MQ=255)                                                                                         tgtgGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGAtctc                                              >  2:74756/1‑140 (MQ=255)                                                                                         tgtgGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGAtctc                                              >  1:977513/1‑140 (MQ=255)                                                                                         tgtgGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGAtct                                               >  2:1170621/1‑139 (MQ=255)                                                                                         tgtgGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGAtct                                               <  1:1170621/139‑1 (MQ=255)                                                                                             gCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCa                                          <  2:409558/140‑1 (MQ=255)                                                                                             gCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCa                                          >  1:598265/1‑140 (MQ=255)                                                                                              cTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAg                                         >  2:91234/1‑140 (MQ=255)                                                                                              cTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAg                                         >  1:1151531/1‑140 (MQ=255)                                                                                                 aCGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCgg                                      >  2:194423/1‑140 (MQ=255)                                                                                                   gggCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGAtctctc                                            >  1:255961/1‑132 (MQ=255)                                                                                                   gggCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGAtctctc                                            <  2:255961/132‑1 (MQ=255)                                                                                                   gggCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGAc                                    >  2:1081675/1‑140 (MQ=255)                                                                                                     gCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACaa                                  >  1:888357/1‑140 (MQ=255)                                                                                                     gCGAAGACCCACCGCAGCGCATTATGCGGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACaa                                  >  1:590191/1‑140 (MQ=255)                                                                                                               aCCGCAGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCggcggc                        >  1:1339143/1‑140 (MQ=255)                                                                                                                    aGCGCATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTAc                   <  2:1021176/140‑1 (MQ=255)                                                                                                                     gcgcATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACa                  >  1:1050976/1‑140 (MQ=255)                                                                                                                     gcgcATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACa                  >  2:1281998/1‑140 (MQ=255)                                                                                                                      cgcATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAg                 >  2:769252/1‑140 (MQ=255)                                                                                                                        cATTATGCTGCGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGtt               <  1:313472/140‑1 (MQ=255)                                                                                                                             tgctgcGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCaaa          <  1:183107/140‑1 (MQ=255)                                                                                                                             tgctgcGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCaaa          <  1:1081675/140‑1 (MQ=255)                                                                                                                             tgctgcGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCaaa          <  1:478300/140‑1 (MQ=255)                                                                                                                                tgcGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAActg       <  1:1281998/140‑1 (MQ=255)                                                                                                                                 gcGTACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAActgc      <  1:483738/140‑1 (MQ=255)                                                                                                                                    tACAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCtgt   >  1:899056/1‑140 (MQ=255)                                                                                                                                     aCAGAGCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCtgtg  <  2:899056/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       CATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTG  >  NC_000913/422500‑422771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑