Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,884,387	T→C	F18L (TTC→CTC)	rpmH →	50S ribosomal subunit protein L34

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,884,387	0	T	C	100.0%	324.3 / NA	88	F18L (TTC→CTC)	rpmH	50S ribosomal subunit protein L34
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/51);  total (37/51)

CATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAG  >  NC_000913/3884252‑3884515                                                                                                                                        |                                                                                                                                 cATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCg                                                                                                                              <  2:130881/140‑1 (MQ=255)  aTGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGt                                                                                                                             <  2:382505/140‑1 (MQ=255)  aTGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGt                                                                                                                             <  2:616686/140‑1 (MQ=255)  aTGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGt                                                                                                                             >  2:1116370/1‑140 (MQ=255)          ggTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTAt                                                                                                                     <  1:20941/140‑1 (MQ=255)          ggTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTAt                                                                                                                     >  1:196930/1‑140 (MQ=255)          ggTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTAt                                                                                                                     >  2:1189707/1‑140 (MQ=255)          ggTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTAt                                                                                                                     >  2:946087/1‑140 (MQ=255)            tgtCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATgg                                                                                                                   <  1:479721/140‑1 (MQ=255)            tgtCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATgg                                                                                                                   <  1:89402/140‑1 (MQ=255)            tgtCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATgg                                                                                                                   >  2:89402/1‑140 (MQ=255)             gtCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCg                                                                                                                              <  2:1129675/128‑1 (MQ=255)             gtCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCg                                                                                                                              >  1:1129675/1‑128 (MQ=255)              tCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTg                                                                                                                            >  2:812183/1‑129 (MQ=255)              tCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTg                                                                                                                            <  1:812183/129‑1 (MQ=255)              tCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGct                                                                                                                 >  1:133531/1‑140 (MQ=255)              tCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGct                                                                                                                 >  2:248249/1‑140 (MQ=255)                cATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGctac                                                                                                               >  2:246707/1‑140 (MQ=255)                   cGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTTAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTaa                                                                                                            >  1:543836/1‑140 (MQ=255)                      ttCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTaaaaa                                                                                                         >  2:270645/1‑140 (MQ=255)                        cATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATg                                                                                                       >  2:1034442/1‑140 (MQ=255)                             ttCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGgtcgt                                                                                                  >  2:799207/1‑140 (MQ=255)                              tCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGgtcgtc                                                                                                 <  2:981641/140‑1 (MQ=255)                              tCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGgtcgtc                                                                                                 <  2:961481/140‑1 (MQ=255)                              tCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAACCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGgtcgtc                                                                                                 <  1:969874/140‑1 (MQ=255)                                ggcggATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAg                                                                                               >  1:309785/1‑140 (MQ=255)                                           aaTAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCAcg                                                                                    <  2:196930/140‑1 (MQ=255)                                            aTAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGgtcgt                                                                                                  <  2:207666/125‑1 (MQ=255)                                            aTAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGgtcgt                                                                                                  >  1:207666/1‑125 (MQ=255)                                            aTAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCAcgt                                                                                   >  1:129160/1‑140 (MQ=255)                                              aaaGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCAcgtcg                                                                                 <  2:1289200/140‑1 (MQ=255)                                              aaaGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCAcgtcg                                                                                 <  2:1350484/140‑1 (MQ=255)                                              aaaGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCAcgtcg                                                                                 <  1:799207/140‑1 (MQ=255)                                                aGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCAcgttgtc                                                                               <  2:718869/140‑1 (MQ=255)                                                aGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCAcgtcgtc                                                                               <  1:240804/140‑1 (MQ=255)                                                    aTTGAATTTATTCAAGTTTAGGTAG‑AATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCt                                                                          >  1:288257/1‑140 (MQ=255)                                                     ttGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCt                                                                          >  1:1146958/1‑140 (MQ=255)                                                     ttGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCt                                                                          >  1:1186967/1‑140 (MQ=255)                                                     ttGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCt                                                                          >  2:799318/1‑140 (MQ=255)                                                      tGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTa                                                                         <  1:246707/140‑1 (MQ=255)                                                          tttATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAgg                                                                     <  2:133531/140‑1 (MQ=255)                                                            tATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCAcgtcg                                                                                 <  2:1168506/126‑1 (MQ=255)                                                            tATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCAcgtcg                                                                                 >  1:1168506/1‑126 (MQ=255)                                                               tCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCcgcg                                                                >  1:672017/1‑140 (MQ=255)                                                               tCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCcgcg                                                                <  2:1186967/140‑1 (MQ=255)                                                                  aGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTc                                                             >  1:1136339/1‑140 (MQ=255)                                                                    tttAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGt                                                           <  1:270645/140‑1 (MQ=255)                                                                      tAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCt                                                         <  1:1034442/140‑1 (MQ=255)                                                                        ggTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCcgcg                                                                <  1:728115/131‑1 (MQ=255)                                                                        ggTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCcgcg                                                                >  1:1296712/1‑131 (MQ=255)                                                                        ggTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCcgcg                                                                >  2:728115/1‑131 (MQ=255)                                                                        ggTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCcgcg                                                                <  2:1296712/131‑1 (MQ=255)                                                                        ggTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGa                                                       <  1:1200818/140‑1 (MQ=255)                                                                        ggTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGa                                                       >  2:257544/1‑140 (MQ=255)                                                                         gTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGAc                                                      <  1:1189707/140‑1 (MQ=255)                                                                            gAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGt                                                   <  1:1344800/140‑1 (MQ=255)                                                                            gAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGt                                                   <  1:248249/140‑1 (MQ=255)                                                                             aaaTCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGtt                                                  >  2:567950/1‑140 (MQ=255)                                                                              aaTCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGttt                                                 <  2:1136339/140‑1 (MQ=255)                                                                              aaTCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTATGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGttt                                                 >  2:1275208/1‑140 (MQ=255)                                                                                 cGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTa                                              <  2:1308388/140‑1 (MQ=255)                                                                                 cGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTa                                              <  1:289561/140‑1 (MQ=255)                                                                                 cGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTa                                              <  1:1116370/140‑1 (MQ=255)                                                                                  gCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGAc                                                      >  2:148165/1‑131 (MQ=255)                                                                                  gCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGAc                                                      <  1:148165/131‑1 (MQ=255)                                                                                  gCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTaa                                             >  2:376363/1‑140 (MQ=255)                                                                                   ccATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGttt                                                 >  2:1251708/1‑135 (MQ=255)                                                                                   ccATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGttt                                                 <  1:1251708/135‑1 (MQ=255)                                                                                   ccATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAg                                            <  1:1275208/140‑1 (MQ=255)                                                                                      tGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGtaa                                         <  2:288257/140‑1 (MQ=255)                                                                                       gAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGtaat                                        <  2:964037/140‑1 (MQ=255)                                                                                             cACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCt                                  <  1:567950/140‑1 (MQ=255)                                                                                             cACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCt                                  <  1:257544/140‑1 (MQ=255)                                                                                                ttttCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAAc                               <  2:129160/140‑1 (MQ=255)                                                                                                      aCCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGa                         <  2:1158253/140‑1 (MQ=255)                                                                                                      aCCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGa                         <  2:309785/140‑1 (MQ=255)                                                                                                      aCCGTCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGa                         <  1:939245/140‑1 (MQ=255)                                                                                                          tCTGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTgg                     <  2:701905/140‑1 (MQ=255)                                                                                                            tGTACTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGtt                   <  1:1111180/140‑1 (MQ=255)                                                                                                               aCTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAg                >  1:1211893/1‑140 (MQ=255)                                                                                                                cTGAAGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGc               <  2:223427/140‑1 (MQ=255)                                                                                                                   aaGCGCAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCg            >  2:1361049/1‑140 (MQ=255)                                                                                                                     gcgcAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGa                         >  1:423248/1‑125 (MQ=255)                                                                                                                     gcgcAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGa                         <  2:423248/125‑1 (MQ=255)                                                                                                                      cgcAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCAt         <  1:518591/140‑1 (MQ=255)                                                                                                                      cgcAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCAt         <  2:543836/140‑1 (MQ=255)                                                                                                                        cAACCGTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCAttt       >  2:801929/1‑140 (MQ=255)                                                                                                                             gTTCTCACGGCCTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAg  >  2:825241/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                 CATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAG  >  NC_000913/3884252‑3884515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑