Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,935,222	C→T	A482V (GCC→GTC)	rbsA →	ribose ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,935,222	0	C	T	100.0%	321.8 / NA	92	A482V (GCC→GTC)	rbsA	ribose ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (49/43);  total (49/43)

TGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGCCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACTGTCTCTGGTCGCCGTTATTTCACGAAAGCGTGGCTGATGGAGCAGAAATCGCTTAT  >  NC_000913/3935087‑3935358                                                                                                                                        |                                                                                                                                         tGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCAcc                                                                                                                                      >  2:383098/1‑140 (MQ=255)   aTTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCa                                                                                                                                    >  1:58152/1‑140 (MQ=255)   aTTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCa                                                                                                                                    >  1:1091696/1‑140 (MQ=255)   aTTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCa                                                                                                                                    <  2:1289483/140‑1 (MQ=255)   aTTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCa                                                                                                                                    >  1:1207137/1‑140 (MQ=255)   aTTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCa                                                                                                                                    >  2:473987/1‑140 (MQ=255)      aaCCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGa                                                                                                                                 >  1:381546/1‑140 (MQ=255)      aaCCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGa                                                                                                                                 >  1:329287/1‑140 (MQ=255)      aaCCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGa                                                                                                                                 <  2:190419/140‑1 (MQ=255)          aGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAgtg                                                                                                                             >  1:402739/1‑140 (MQ=255)           gTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAgtgt                                                                                                                            >  1:623950/1‑140 (MQ=255)             tCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTa                                                                                                                          >  2:496422/1‑140 (MQ=255)               aaaGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAAt                                                                                                                        <  2:881397/140‑1 (MQ=255)                 aGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATgg                                                                                                                      >  1:168180/1‑140 (MQ=255)                      aTGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGcc                                                                                                                 >  1:1187680/1‑140 (MQ=255)                      aTGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGcc                                                                                                                 >  1:612295/1‑140 (MQ=255)                      aTGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGcc                                                                                                                 >  1:1167739/1‑140 (MQ=255)                      aTGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGcc                                                                                                                 >  1:1250691/1‑140 (MQ=255)                             gAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTgg                                                                                                          >  2:1254310/1‑140 (MQ=255)                              aGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTggg                                                                                                         <  2:1087951/140‑1 (MQ=255)                                   catTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGc                                                                                                    >  1:1034917/1‑140 (MQ=255)                                        tGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAAt                                                                                               <  2:168180/140‑1 (MQ=255)                                         ggTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATc                                                                                              >  1:72687/1‑140 (MQ=255)                                            gtCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATcgcg                                                                                           <  2:72687/140‑1 (MQ=255)                                            gtCATCGGAGATGCCAGAAGTATTAGGCATGACCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGATGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATcgcg                                                                                           <  2:1207137/140‑1 (MQ=255)                                             tCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGc                                                                                                        >  2:824427/1‑126 (MQ=255)                                             tCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGc                                                                                                        <  1:824427/126‑1 (MQ=255)                                             tCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGt                                                                                          >  2:772468/1‑140 (MQ=255)                                               aTCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGa                                                                                        >  2:615198/1‑140 (MQ=255)                                               aTCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGa                                                                                        >  2:143087/1‑140 (MQ=255)                                                tCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGaa                                                                                       <  1:383098/140‑1 (MQ=255)                                                    agaTGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAGGCTTAATCGCGTGAATCAg                                                                                   <  2:1091696/140‑1 (MQ=255)                                                     gaTGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAgg                                                                                  >  1:894339/1‑140 (MQ=255)                                                       tGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAAt                                                                                      >  1:1256822/1‑134 (MQ=255)                                                       tGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAAt                                                                                      <  2:1256822/134‑1 (MQ=255)                                                         ccAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTa                                                                              >  2:1099959/1‑140 (MQ=255)                                                            gAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTaaaa                                                                           <  2:58152/140‑1 (MQ=255)                                                                 aTTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATg                                                                      <  2:331075/140‑1 (MQ=255)                                                                   tAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGAc                                                                    <  2:491237/140‑1 (MQ=255)                                                                       cATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGa                                                                     >  1:478785/1‑135 (MQ=255)                                                                       cATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGa                                                                     <  2:478785/135‑1 (MQ=255)                                                                       cATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAAcc                                                                >  2:178703/1‑140 (MQ=255)                                                                         tGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCa                                                              >  1:1360950/1‑140 (MQ=255)                                                                          gAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGAc                                                                    >  2:1004948/1‑133 (MQ=255)                                                                          gAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGAc                                                                    <  1:1004948/133‑1 (MQ=255)                                                                          gAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAg                                                             >  2:1186413/1‑140 (MQ=255)                                                                          gAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAg                                                             >  2:282809/1‑140 (MQ=255)                                                                          gAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAg                                                             >  2:751106/1‑140 (MQ=255)                                                                           aGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGa                                                                     <  1:47412/131‑1 (MQ=255)                                                                           aGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGa                                                                     >  2:47412/1‑131 (MQ=255)                                                                           aGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGa                                                            <  2:329287/140‑1 (MQ=255)                                                                           aGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGa                                                            <  2:866391/140‑1 (MQ=255)                                                                           aGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGa                                                            <  2:921202/140‑1 (MQ=255)                                                                           aGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGa                                                            <  1:751106/140‑1 (MQ=255)                                                                            gCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGAc                                                           <  1:258639/140‑1 (MQ=255)                                                                             cGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACt                                                          >  2:1348963/1‑140 (MQ=255)                                                                                tCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGa                                                            >  1:746218/1‑135 (MQ=255)                                                                                tCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGa                                                            <  2:746218/135‑1 (MQ=255)                                                                                 cGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACTGtct                                                      >  2:731782/1‑140 (MQ=255)                                                                                     tcatcGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACTGTCTCTgg                                                  <  1:731782/140‑1 (MQ=255)                                                                                          gTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACTGTCTCTGGTCGcc                                             <  1:1254310/140‑1 (MQ=255)                                                                                            catgcatgAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACTGTCTCTGGTCGCCGt                                           <  1:473987/140‑1 (MQ=255)                                                                                              tgcatgAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACTGTCTCTGGTCGCCGTTa                                         >  1:648584/1‑140 (MQ=255)                                                                                              tgcatgAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACTGTCTCTGGTCGCCGTTa                                         >  1:50744/1‑140 (MQ=255)                                                                                                catgAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACTGTCTCTGGTCGCCGTTAtt                                       <  1:496422/140‑1 (MQ=255)                                                                                                catgAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACTGTCTCTGGTCGCCGTTAtt                                       <  1:152628/140‑1 (MQ=255)                                                                                                  tgAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACTGTCTCTGGTCGCCGTTATTTc                                     >  1:48490/1‑140 (MQ=255)                                                                                                    aaGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACTGTCTCTGGTCGCCGTTATTTCAc                                   <  1:934173/140‑1 (MQ=255)                                                                                                    aaGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACTGTCTCTGGTCGCCGTTATTTCAc                                   <  2:612295/140‑1 (MQ=255)                                                                                                    aaGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACTGTCTCTGGTCGCCGTTATTTCAc                                   <  2:1167739/140‑1 (MQ=255)                                                                                                     aGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACTGTCTCTGGTCGCCGTTAtt                                       <  1:1241404/135‑1 (MQ=255)                                                                                                     aGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACTGTCTCTGGTCGCCGTTAtt                                       >  2:1241404/1‑135 (MQ=255)                                                                                                     aGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACTGTCTCTGGTCGCCGTTATTTCAc                                   <  1:855823/139‑1 (MQ=255)                                                                                                     aGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACTGTCTCTGGTCGCCGTTATTTCAc                                   >  2:855823/1‑139 (MQ=255)                                                                                                     aGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACTGTCTCTGGTCGCCGTTATTTCACg                                  <  1:615198/140‑1 (MQ=255)                                                                                                     aGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACTGTCTCTGGTCGCCGTTATTTCACg                                  >  2:331716/1‑140 (MQ=255)                                                                                                     aGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACTGTCTCTGGTCGCCGTTATTTCACg                                  <  2:402739/140‑1 (MQ=255)                                                                                                      gggCATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACTGTCTCTGGTCGCCGTTATTTCACGa                                 >  2:1111967/1‑140 (MQ=255)                                                                                                         cATCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACTGTCTCTGGTCGCCGTTATTTCACGAAAg                              >  2:1327805/1‑140 (MQ=255)                                                                                                          aTCTCAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACTGTCTCTGGTCGCCGTTATTTCACGAAAGc                             >  1:634416/1‑140 (MQ=255)                                                                                                           tctcAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACTGTCTCTGGTCGCCGTTATTTCACGAAAGCg                            >  1:404889/1‑140 (MQ=255)                                                                                                            ctcAGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACTGTCTCTGGTCGCCGTTATTTCACGAAAGCGt                           <  2:404889/140‑1 (MQ=255)                                                                                                               aGCGGGGAATTTACTCGTGAGCAGGTCACCCAGGAGGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACTGTCTCTGGTCGCCGTTATTTCACGAAAGCGTGGc                        >  2:345972/1‑140 (MQ=255)                                                                                                                  ggggAATTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACTGTCTCTGGTCGCCGTTATTTCACGAAAGCGTGGCTGa                     <  2:634416/140‑1 (MQ=255)                                                                                                                       aTTTACTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACTGTCTCTGGTCGCCGTTATTTCACGAAAGCGTGGCTGATGGAg                >  2:175132/1‑140 (MQ=255)                                                                                                                            cTCGTGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACTGTCTCTGGTCGCCGTTATTTCACGAAAGCGTGGCTGATGGAGCAGaa           <  2:1360950/140‑1 (MQ=255)                                                                                                                                tGAGCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACTGTCTCTGGTCGCCGTTATTTCACGAAAGCGTGGCTGATGGAGCAGAAATCg       >  1:258493/1‑140 (MQ=255)                                                                                                                                  aGCAGGTCACCCAGGAGGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACTGTCTCTGGTCGCCGTTATTTCACGAAAGCGTGGCTGATGGAGCAGAAATCGCt     <  1:345972/140‑1 (MQ=255)                                                                                                                                   gCAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACTGTCTCTGGTCGCCGTTATTTCACGAAAGCGTGGCTGATGGAGCAGAAATCGCtt    <  1:282809/140‑1 (MQ=255)                                                                                                                                    cAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACTGTCTCTGGTCGCCGTTATTTCACGAAAGCGTGGCTGATGGAGCAGAAATCGCTTa   <  2:50744/140‑1 (MQ=255)                                                                                                                                    cAGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACTGTCTCTGGTCGCCGTTATTTCACGAAAGCGTGGCTGATGGAGCAGAAATCGCTTa   <  1:780267/140‑1 (MQ=255)                                                                                                                                     aGGTCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACTGTCTCTGGTCGCCGTTATTTCACGAAAGCGTGGCTGATGGAGCAGAAATCGCTTAt  <  1:1327805/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         TGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTACTCGTGAGCAGGCCACCCAGGAAGTGTTAATGGCTGCCGCTGTGGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACTGTCTCTGGTCGCCGTTATTTCACGAAAGCGTGGCTGATGGAGCAGAAATCGCTTAT  >  NC_000913/3935087‑3935358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑