Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,009,923	T→C	L252L (TTA→CTA)	pldB →	lysophospholipase L2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,009,923	0	T	C	100.0%	365.3 / NA	99	L252L (TTA→CTA)	pldB	lysophospholipase L2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (50/49);  total (50/49)

GCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGTTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACG  >  NC_000913/4009788‑4010056                                                                                                                                        |                                                                                                                                      gCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGc                                                                                                                                   <  1:1330036/140‑1 (MQ=255)      cAACGTACTGACCCACAGCAGACAGTGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTg                                                                                                                              <  2:417885/140‑1 (MQ=255)        aCGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCg                                                                                                                            <  1:292482/140‑1 (MQ=255)         cGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCgg                                                                                                                           <  2:1059795/140‑1 (MQ=255)              tGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGtgat                                                                                                                      <  2:599505/140‑1 (MQ=255)               gACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGtgatg                                                                                                                     >  1:750646/1‑140 (MQ=255)               gACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGtgatg                                                                                                                     >  2:372393/1‑140 (MQ=255)               gACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGtgatg                                                                                                                     >  2:356392/1‑140 (MQ=255)               gACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGACGAACAGGTGCTAGCCGGTGCGGGtgatg                                                                                                                     >  2:188990/1‑140 (MQ=255)                aCCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGtgatga                                                                                                                    >  1:1171438/1‑140 (MQ=255)                 cccACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGac                                                                                                                   >  1:1351933/1‑140 (MQ=255)                   cacaGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGacgc                                                                                                                 >  2:717376/1‑140 (MQ=255)                    acaGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGacgcc                                                                                                                >  1:1212357/1‑140 (MQ=255)                     cagcagACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTTGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGacgcca                                                                                                               >  1:309145/1‑140 (MQ=255)                     cagcagACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGacgcca                                                                                                               >  1:645688/1‑140 (MQ=255)                      agcagACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGacgccac                                                                                                              >  2:1051102/1‑140 (MQ=255)                      agcagACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGacgccac                                                                                                              >  1:147230/1‑140 (MQ=255)                        cagACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGacgccacgc                                                                                                            <  1:571559/140‑1 (MQ=255)                         agACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGacgccacgcc                                                                                                           <  1:29610/140‑1 (MQ=255)                           aCAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCaa                                                                                                         >  1:962055/1‑140 (MQ=255)                             aGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAAc                                                                                                        >  2:627136/1‑139 (MQ=255)                             aGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAAc                                                                                                        <  1:627136/139‑1 (MQ=255)                             aGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACg                                                                                                       <  2:147230/140‑1 (MQ=255)                             aGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACg                                                                                                       >  2:14310/1‑140 (MQ=255)                                gATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCtt                                                                                                    >  2:91548/1‑140 (MQ=255)                                   atCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTtctc                                                                                                 <  1:356392/140‑1 (MQ=255)                                     cGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCtt                                                                                               <  2:499323/140‑1 (MQ=255)                                     cGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTG‑TGACGCCACGCCAACGCTTCTCTTg                                                                                              <  1:146134/140‑1 (MQ=255)                                          tAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAgg                                                                                          <  2:399229/140‑1 (MQ=255)                                          tAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAgg                                                                                          <  2:494059/140‑1 (MQ=255)                                          tAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAgg                                                                                          <  2:99943/140‑1 (MQ=255)                                          tAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAgg                                                                                          <  1:188990/140‑1 (MQ=255)                                            aCTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCa                                                                                            <  1:466630/136‑1 (MQ=255)                                            aCTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCa                                                                                            >  2:466630/1‑136 (MQ=255)                                            aCTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCt                                                                                        <  1:677415/140‑1 (MQ=255)                                            aCTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCt                                                                                        >  1:713648/1‑140 (MQ=255)                                               tACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGaa                                                                                     <  1:450923/140‑1 (MQ=255)                                                aCGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAag                                                                                    >  1:1041272/1‑140 (MQ=255)                                                aCGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAag                                                                                    >  1:1025282/1‑140 (MQ=255)                                                 cGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAaga                                                                                   <  2:1187468/140‑1 (MQ=255)                                                  gCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAagag                                                                                  >  2:1071557/1‑140 (MQ=255)                                                      cTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAAc                                                                              <  2:713648/140‑1 (MQ=255)                                                      cTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAAc                                                                              <  2:1041272/140‑1 (MQ=255)                                                      cTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAAc                                                                              >  2:1027031/1‑140 (MQ=255)                                                       tATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAAcg                                                                             <  1:1164439/140‑1 (MQ=255)                                                       tATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAAcg                                                                             >  2:455960/1‑140 (MQ=255)                                                        aTGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGc                                                                                         >  1:835602/1‑127 (MQ=255)                                                        aTGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGc                                                                                         <  2:835602/127‑1 (MQ=255)                                                        aTGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAAcgc                                                                            <  1:661753/140‑1 (MQ=255)                                                         tGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAAcgcg                                                                           <  1:1329233/140‑1 (MQ=255)                                                         tGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAAcgcg                                                                           >  2:902339/1‑140 (MQ=255)                                                         tGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAAcgcg                                                                           >  1:1043156/1‑140 (MQ=255)                                                         tGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAAcgcg                                                                           <  2:1043156/140‑1 (MQ=255)                                                           cTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCgtg                                                                         <  2:485269/140‑1 (MQ=255)                                                              atgaCCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAAcgcg                                                                           >  2:40471/1‑135 (MQ=255)                                                              atgaCCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAAcgcg                                                                           <  2:311099/135‑1 (MQ=255)                                                              atgaCCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAAcgcg                                                                           >  1:311099/1‑135 (MQ=255)                                                              atgaCCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAAcgcg                                                                           <  1:40471/135‑1 (MQ=255)                                                              atgaCCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCgtggtg                                                                      >  2:793273/1‑140 (MQ=255)                                                              atgaCCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCgtggtg                                                                      >  1:1253586/1‑140 (MQ=255)                                                              atgaCCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCgtggtg                                                                      >  2:20588/1‑140 (MQ=255)                                                              atgaCCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCgtggtg                                                                      >  1:432354/1‑140 (MQ=255)                                                              atgaCCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCgtggtg                                                                      >  1:318057/1‑140 (MQ=255)                                                              atgaCCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCgtggtg                                                                      >  1:36377/1‑140 (MQ=255)                                                                 aCCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGAt                                                                   >  2:299854/1‑140 (MQ=255)                                                                 aCCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGAt                                                                   <  1:902339/140‑1 (MQ=255)                                                                 aCCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGAt                                                                   <  1:1051102/140‑1 (MQ=255)                                                                 aCCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGAt                                                                   <  2:1025282/140‑1 (MQ=255)                                                                     aaCGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAAcc                                                               <  1:14310/140‑1 (MQ=255)                                                                             gcgTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCgcatgcat                                                       >  2:1152737/1‑140 (MQ=255)                                                                               gTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGa                                                     <  2:1351933/140‑1 (MQ=255)                                                                                tCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGAc                                                    <  1:299854/140‑1 (MQ=255)                                                                                  ggTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCg                                                  <  1:793273/140‑1 (MQ=255)                                                                                              tACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATACCCGCATGCATGACCGTTTTTGTGAACt                                      <  2:318057/140‑1 (MQ=255)                                                                                              tACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACt                                      >  1:403531/1‑140 (MQ=255)                                                                                                    ttggTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCAc                                <  2:309145/138‑1 (MQ=255)                                                                                                      ggTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCAccg                              >  2:509590/1‑140 (MQ=255)                                                                                                      ggTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCAccg                              >  1:932206/1‑140 (MQ=255)                                                                                                      ggTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCAccg                              >  1:391073/1‑140 (MQ=255)                                                                                                      ggTACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCAccg                              >  2:1223147/1‑140 (MQ=255)                                                                                                        tACGCGAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCAccgcc                            >  2:655786/1‑140 (MQ=255)                                                                                                          cgcgAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGTACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACcgccgc                          >  2:1023529/1‑140 (MQ=255)                                                                                                          cgcgAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACcgccgc                          >  1:866737/1‑140 (MQ=255)                                                                                                          cgcgAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACcgccgc                          >  2:478932/1‑140 (MQ=255)                                                                                                          cgcgAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACcgccgc                          >  1:1302434/1‑140 (MQ=255)                                                                                                          cgcgAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACcgccgc                          >  1:735865/1‑140 (MQ=255)                                                                                                          cgcgAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACcgccgc                          >  2:283396/1‑140 (MQ=255)                                                                                                          cgcgAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATACATGACCGTTTTTGTGAACTCCGCACcgccgc                          >  1:1049334/1‑140 (MQ=255)                                                                                                           gcgAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCcgcg                         <  2:1212357/140‑1 (MQ=255)                                                                                                             gAAAGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCggg                       <  1:455960/140‑1 (MQ=255)                                                                                                               aaGTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCGGGcc                     <  2:1366540/140‑1 (MQ=255)                                                                                                                 gTATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCGGGCCAt                   <  1:692983/140‑1 (MQ=255)                                                                                                                  tATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGGTAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCGGGCCATc                  >  1:1214766/1‑140 (MQ=255)                                                                                                                  tATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCGGGCCATc                  <  1:283396/140‑1 (MQ=255)                                                                                                                  tATTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCGGGCCATc                  <  1:692594/140‑1 (MQ=255)                                                                                                                   aTTCTGGCTGGCGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCGGGCCATcc                 <  2:727213/140‑1 (MQ=255)                                                                                                                         gctggcGAACAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCGGGCCATCCTGTCGa           >  2:900854/1‑140 (MQ=255)                                                                                                                                aaCAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCGGGCCATCCTGTCGAAggagga    <  2:645688/140‑1 (MQ=255)                                                                                                                                aaCAGGTGCTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCGGGCCATCCTGTCGAAggagga    <  1:1023529/140‑1 (MQ=255)                                                                                                                                  cAGGTGCTAGCCGGTGCGGGTGATGACGTCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACg  <  2:503175/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      GCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGTTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGCTGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACG  >  NC_000913/4009788‑4010056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑