Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	442,284	C→T	P246S (CCG→TCG)	thiI →	tRNA uridine 4‑sulfurtransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	442,284	0	C	T	100.0%	191.2 / NA	54	P246S (CCG→TCG)	thiI	tRNA uridine 4‑sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/26);  total (28/26)

CCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGTTACGGCGTACAGGCGCTGGT  >  NC_000913/442154‑442402                                                                                                                                   |                                                                                                                       ccGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGtcgtcg                                                                                                               >  1:18220/1‑140 (MQ=255)     gTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGa                                                                                                           <  1:454611/140‑1 (MQ=255)        cATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAAt                                                                                                        >  2:32070/1‑140 (MQ=255)                cttcttTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATtct                                                                                                     >  1:111815/1‑135 (MQ=255)                cttcttTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATtct                                                                                                     <  2:111815/135‑1 (MQ=255)                cttcttTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCgaga                                                                                                >  1:108454/1‑140 (MQ=255)                cttcttTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCgaga                                                                                                >  2:1144092/1‑140 (MQ=255)                  tcttTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGaaa                                                                                              >  2:849142/1‑140 (MQ=255)                     ttAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATc                                                                                           >  1:573950/1‑140 (MQ=255)                      tAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCgag                                                                                                 <  1:480452/133‑1 (MQ=255)                      tAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCgag                                                                                                 >  2:480452/1‑133 (MQ=255)                       aaCCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATcga                                                                                         <  1:240788/140‑1 (MQ=255)                         ccTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATcgacg                                                                                       <  2:108454/140‑1 (MQ=255)                         ccTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATcgacg                                                                                       <  2:475038/140‑1 (MQ=255)                            cggcggCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGacgacg                                                                                    >  1:1157864/1‑140 (MQ=255)                            cggcggCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGacgacg                                                                                    >  2:989047/1‑140 (MQ=255)                             ggcggcGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATcgac                                                                                        <  2:403463/135‑1 (MQ=255)                             ggcggcGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATcgac                                                                                        >  1:403463/1‑135 (MQ=255)                             ggcggcGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACgg                                                                                   >  1:708823/1‑140 (MQ=255)                              gcggcgcggcGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGt                                                                                  <  2:1149503/140‑1 (MQ=255)                               cggcgcggcgCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTc                                                                                 >  1:1088478/1‑140 (MQ=255)                               cggcgcggcgCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTc                                                                                 >  1:1315538/1‑140 (MQ=255)                                   gcggcgcATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGAt                                                                             <  1:681937/140‑1 (MQ=255)                                     ggcgcATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATgg                                                                           <  1:989047/140‑1 (MQ=255)                                        gcATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCg                                                                        <  1:1144092/140‑1 (MQ=255)                                        gcATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCg                                                                        <  2:598199/140‑1 (MQ=255)                                        gcATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCg                                                                        <  2:408282/140‑1 (MQ=255)                                        gcATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCg                                                                        <  1:32070/140‑1 (MQ=255)                                        gcATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCCTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCg                                                                        <  2:1088478/140‑1 (MQ=255)                                           tGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTa                                                                     <  2:1134933/140‑1 (MQ=255)                                                  ggCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCaa                                                              <  2:1315538/140‑1 (MQ=255)                                                  ggCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCaa                                                              <  1:647101/140‑1 (MQ=255)                                                      tgcgtCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCt                                                                 <  1:625383/133‑1 (MQ=255)                                                      tgcgtCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCt                                                                 >  2:625383/1‑133 (MQ=255)                                                        cgtCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTat                                                        >  1:248938/1‑140 (MQ=255)                                                             ggTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATgg                                                   >  2:1343141/1‑140 (MQ=255)                                                                        aTCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCa                                        <  2:1157864/140‑1 (MQ=255)                                                                           tgtgGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCt                                     >  1:335565/1‑140 (MQ=255)                                                                                  ccGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTgg                              >  1:734336/1‑140 (MQ=255)                                                                                  ccGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTgg                              >  1:214387/1‑140 (MQ=255)                                                                                  ccGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTgg                              <  2:1073194/140‑1 (MQ=255)                                                                                  ccGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTgg                              >  1:416242/1‑140 (MQ=255)                                                                                  ccGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTgg                              >  1:430174/1‑140 (MQ=255)                                                                                    gCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCt                            <  2:18220/140‑1 (MQ=255)                                                                                    gCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCt                            <  2:416242/140‑1 (MQ=255)                                                                                                   aCCGCGTGCGTTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGTTACGGCGTa             >  1:935701/1‑140 (MQ=255)                                                                                                       cgtgcgtTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGTTGGGCGTTATCCTCAAACGTATGATGCTGCGTGCCGCATCTAAAGTGGCTGAACGTTACGGCGTACAgg         >  2:1222011/1‑140 (MQ=255)                                                                                                        gtgcgtTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGTTACg                  <  1:876430/130‑1 (MQ=255)                                                                                                        gtgcgtTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGTTACg                  >  2:876430/1‑130 (MQ=255)                                                                                                        atgcgtTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGTTACGGCGTACAGgc        >  2:730084/2‑140 (MQ=255)                                                                                                         tgcgtTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGTTa                    >  1:877833/1‑127 (MQ=255)                                                                                                         tgcgtTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGTTa                    <  2:877833/127‑1 (MQ=255)                                                                                                         tgcgtTTTGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGTTACGGCGTACAGgcg       >  1:755334/1‑140 (MQ=255)                                                                                                              tttGTCGCTATTAATTTCGAATCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGTTACGGCGTACAGGCGCTGGt  <  2:935701/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                       CCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGTTACGGCGTACAGGCGCTGGT  >  NC_000913/442154‑442402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑