Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,141,044	G→A	A393V (GCG→GTG)	ptsA ←	putative PTS multiphosphoryl transfer protein PtsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,141,044	0	G	A	100.0%	302.9 / NA	86	A393V (GCG→GTG)	ptsA	putative PTS multiphosphoryl transfer protein PtsA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (42/44);  total (42/44)

GTCCGTTGGCGGATTCCAGCGCCTGACAAAAAATGTTGTACAACTCGCTTTCGCCCGGTGCGCTGGTGCGATCCATATAGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCGCTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGTTGCTCACGCAGGGCGTCCTGTACGCGGGCTTCTTGCTGATAATAACGA  >  NC_000913/4140907‑4141178                                                                                                                                          |                                                                                                                                       gTCCGTTGGCGGATTCCAGCGCCTGACAAAAAATGTTGTACAACTCGCTTTCGCCCGGTGCGCTGGTGCGATCCATATAGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACt                                                                                                                                      <  1:15245/140‑1 (MQ=255)  tCCGTTGGCGGATTCCAGCGCCTGACAAAAAATGTTGTACAACTCGCTTTCGCCCGGTGCGCTGGTGCGATCCATATAGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACtt                                                                                                                                     >  1:720023/1‑140 (MQ=255)  tCCGTTGGCGGATTCCAGCGCCTGACAAAAAATGTTGTACAACTCGCTTTCGCCCGGTGCGCTGGTGCGATCCATATAGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACtt                                                                                                                                     >  2:856256/1‑140 (MQ=255)     gTTGGCGGATTCCAGCGCCTGACAAAAAATGTTGTACAACTCGCTTTCGCCCGGTGCGCTGGTGCGATCCATATAGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCa                                                                                                                                  <  1:1103883/140‑1 (MQ=255)     gTTGGCGGATTCCAGCGCCTGACAAAAAATGTTGTACAACTCGCTTTCGCCCGGTGCGCTGGTGCGATCCATATAGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCa                                                                                                                                  <  1:1282010/140‑1 (MQ=255)      ttGGCGGATTCCAGCGCCTGACAAAAAATGTTGTACAACTCGCTTTCGCCCGGTGCGCTGGTGCGATCCATATAGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCAc                                                                                                                                 >  1:298145/1‑140 (MQ=255)      ttGGCGGATTCCAGCGCCTGACAAAAAATGTTGTACAACTCGCTTTCGCCCGGTGCGCTGGTGCGATCCATATAGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCAc                                                                                                                                 <  2:147754/140‑1 (MQ=255)         gCGGATTCCAGCGCCTGACAAAAAATGTTGTACAACTCGCTTTCGCCCGGTGCGCTGGTGCGATCCATATAGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGa                                                                                                                              <  1:1138350/140‑1 (MQ=255)              ttCCAGCGCCTGACAAAAAATGTTGTACAACTCGCTTTCGCCCGGTGCGCTGGTGCGATCCATATAGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAg                                                                                                                         <  2:720023/140‑1 (MQ=255)               tCCAGCGCCTGACAAAAAATGTTGTACAACTCGCTTTCGCCCGGTGCGCTGGTGCGATCCATATAGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGc                                                                                                                        >  2:899548/1‑140 (MQ=255)                  aGCGCCTGACAAAAAATGTTGTACAACTCGCTTTCGCCCGGCGCGCTGGTGCGATCCATATAGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGAt                                                                                                                     >  2:120490/1‑140 (MQ=255)                   gcgcCTGACAAAAAATGTTGTACAACTCGCTTTCGCCCGGTGCGCTGGTGCGATCCATATAGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATg                                                                                                                    >  2:644798/1‑140 (MQ=255)                   gcgcCTGACAAAAAATGTTGTACAACTCGCTTTCGCCCGGTGCGCTGGTGCGATCCATATAGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATg                                                                                                                    <  2:135160/140‑1 (MQ=255)                     gcCTGACAAAAAATGTTGTACAACTCGCTTTCGCCCGGTGCGCTGGTGCGATCCATATAGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGtt                                                                                                                  <  2:746784/140‑1 (MQ=255)                      ccTGACAAAAAATGTTGTACAACTCGCTTTCGCCCGGTGCGCTGGTGCGATCCATATAGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTa                                                                                                                 <  2:1174431/140‑1 (MQ=255)                       cTGACAAAAAATGTTGTACAACTCGCTTTCGCCCGGTGCGCTGGTGCGATCCATATAGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGTGGCCTGCACTTCCACGGAGTGAGCGATGTTAg                                                                                                                >  1:660754/1‑140 (MQ=255)                       cTGACAAAAAATGTTGTACAACTCGCTTTCGCCCGGTGCGCTGGTGCGATCCATATAGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAg                                                                                                                >  1:228893/1‑140 (MQ=255)                          aCAAAAAATGTTGTACAACTCGCTTTCGCCCGGCGCGCTGGTGCGATCCATATAGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCgg                                                                                                             <  1:120490/140‑1 (MQ=255)                                    ttgtACAACTCGCTTTCGCCCGGTGCGCTGGTGCGATCCATATAGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAAt                                                                                                   >  1:567715/1‑140 (MQ=255)                                       tACAACTCGCTTTCGCCCGGTGCGCTGGTGCGATCCATATAGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCg                                                                                                <  1:856256/140‑1 (MQ=255)                                         cAACTCGCTTTCGCCCGGTGCGCTGGTGCGATCCATATAGAGCATTTCAGTGCGGAACAAAGCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGa                                                                                              >  2:1220840/1‑140 (MQ=255)                                            cTCGCTTTCGCCCGGTGCGCTGGTGCGATCCATATAGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATAc                                                                                           >  2:785464/1‑140 (MQ=255)                                              cGCTTTCGCCCGGTGCGCTGGTGCGATCCATATAGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCg                                                                                         >  2:440797/1‑140 (MQ=255)                                                  ttCGCCCGGTGCGCTGGTGCGATCCATATAGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGTAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAg                                                                                     <  2:1139613/140‑1 (MQ=255)                                                  ttCGCCCGGTGCGCTGGTGCGATCCATATAGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAg                                                                                     <  2:109224/140‑1 (MQ=255)                                                  ttCGCCCGGTGCGCTGGTGCGATCCATATAGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAg                                                                                     <  1:1018443/140‑1 (MQ=255)                                                  ttCGCCCGGTGCGCTGGTGCGATCCATATAGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAg                                                                                     <  1:539802/140‑1 (MQ=255)                                                       ccGGTGCGCTGGTGCGATCCATATAGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTa                                                                                <  2:951852/140‑1 (MQ=255)                                                         ggTGCGCTGGTGCGATCCATATAGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACg                                                                              <  2:567715/140‑1 (MQ=255)                                                         ggTGCGCTGGTGCGATCCATATAGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACg                                                                              <  1:899548/140‑1 (MQ=255)                                                           tGCGCTGGTGCGATCCATATAGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACggg                                                                            >  1:1089768/1‑140 (MQ=255)                                                               cTGGTGCGATCCATATAGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCttg                                                                        >  1:1205547/1‑140 (MQ=255)                                                                       aTCCATATAGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCa                                                                >  1:1078197/1‑140 (MQ=255)                                                                       aTCCATATAGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCa                                                                >  1:674789/1‑140 (MQ=255)                                                                            tataGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAg                                                           >  1:1317836/1‑140 (MQ=255)                                                                             ataGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGTGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGa                                                          <  2:660754/140‑1 (MQ=255)                                                                              taGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGac                                                         <  2:298145/140‑1 (MQ=255)                                                                               agagCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGaca                                                        <  2:1089768/140‑1 (MQ=255)                                                                                gagCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGacac                                                       <  1:815242/140‑1 (MQ=255)                                                                                 agCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACg                                                      >  2:1080549/1‑140 (MQ=255)                                                                                 agCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACg                                                      >  2:1348803/1‑140 (MQ=255)                                                                                 agCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACg                                                      >  1:878792/1‑140 (MQ=255)                                                                                 agCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACg                                                      >  2:347061/1‑140 (MQ=255)                                                                                 agCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGACGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACg                                                      >  2:939389/1‑140 (MQ=255)                                                                                  gCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGCATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGc                                                     >  2:939033/1‑140 (MQ=255)                                                                                  gCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGGCACGc                                                     >  1:1308373/1‑140 (MQ=255)                                                                                  gCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGc                                                     >  2:241149/1‑140 (MQ=255)                                                                                   cATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCt                                                    >  1:1112199/1‑140 (MQ=255)                                                                                   cATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCt                                                    >  1:634232/1‑140 (MQ=255)                                                                                    aTTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTg                                                   >  2:479831/1‑140 (MQ=255)                                                                                      ttCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGtt                                                 >  1:1128826/1‑140 (MQ=255)                                                                                      ttCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGtt                                                 >  1:594977/1‑140 (MQ=255)                                                                                                 aCAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGTTGCTCACGCAgg                                      <  1:818658/140‑1 (MQ=255)                                                                                                   aaaCCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGTTGCTCACGCAGGGc                                    <  1:1348803/140‑1 (MQ=255)                                                                                                   aaaCCAACGCCTTCCGCACCATTGACGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGTTGCTCACGCAGGGc                                    <  1:939389/140‑1 (MQ=255)                                                                                                    aaCCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGTTGCTCACGCAGGGCg                                   <  1:347061/140‑1 (MQ=255)                                                                                                    aaCCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGTTGCTCACGCAGGGCg                                   <  1:644798/140‑1 (MQ=255)                                                                                                     aCCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGTTGCTCACGCAGGGCGt                                  >  2:1355838/1‑140 (MQ=255)                                                                                                         aCGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGTTGCTCACGCAGGGCGTCCTg                              <  1:785464/140‑1 (MQ=255)                                                                                                          cGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGTTGCTCAc                                           >  1:595679/1‑126 (MQ=255)                                                                                                          cGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGTTGCTCACGCAGGGCGTCCTGt                             >  2:882044/1‑140 (MQ=255)                                                                                                          cGCCTTCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACAGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGTTGCTCAc                                           <  2:595679/126‑1 (MQ=255)                                                                                                               tCCGCACCATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGTTGCTCACGCAGGGCGTCCTGTAcgcg                        >  2:1014458/1‑140 (MQ=255)                                                                                                                     ccATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGTTGCTCACGCAGGGCGTCCTGTACGCGGGcttc                  <  2:826090/140‑1 (MQ=255)                                                                                                                     ccATTGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGTTGCTCACGCAGGGCGTCCTGTACGCGGGcttc                  <  1:440797/140‑1 (MQ=255)                                                                                                                        ttGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGTTGCTCACGCAGGGCGTCCTGTACGCGGGCTTCTTg               <  2:886118/140‑1 (MQ=255)                                                                                                                         tGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTTAGCCAGACACGCTGTTGCTCACGCAGGGCGTCCTGTACGCGGGCTTCTTGc              <  1:1056126/140‑1 (MQ=255)                                                                                                                         tGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGTTGCTCACGCAGGGCGTcc                                <  1:1072437/122‑1 (MQ=255)                                                                                                                         tGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGTTGCTCACGCAGGGCGTcc                                >  2:1072437/1‑122 (MQ=255)                                                                                                                         tGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGTTGCTCACGCAGGGCGTCCTGTACGCGGGcttctt                <  1:758201/138‑1 (MQ=255)                                                                                                                         tGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGTTGCTCACGCAGGGCGTCCTGTACGCGGGcttctt                >  2:758201/1‑138 (MQ=255)                                                                                                                         tGCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGTTGCTCACGCAGGGCGTCCTGTACGCGGGCTTCTTGc              <  2:841358/140‑1 (MQ=255)                                                                                                                          gCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGTTGCTCACGCAGGGCGTCCTGTACGCGGGCTTCTTGCt             <  1:1220840/140‑1 (MQ=255)                                                                                                                          gCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGTTGCTCACGCAGGGCGTCCTGTACGCGGGCTTCTTGCt             <  1:1080549/140‑1 (MQ=255)                                                                                                                          gCCGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGTTGCTCACGCAGGGCGTCCTGTACGCGGGCTTCTTGCt             <  1:1355838/140‑1 (MQ=255)                                                                                                                            cGAATGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGTTGCTCACGCAGGGCGTCCTGTACGCGGGCTTCTTGCTGa           <  2:672594/140‑1 (MQ=255)                                                                                                                              aaTGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGTTGCTCACGCAGGGCGTCCTGTACGCGGGCTTCTTGCTGata         >  1:311326/1‑140 (MQ=255)                                                                                                                               aTGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGTTGCTCACGCAGGGCGTCCTGTACGCGGGCTTCTTGCTGataa        <  2:1078197/140‑1 (MQ=255)                                                                                                                               aTGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGTTGCTCACGCAGGGCGTCCTGTACGCGGGCTTCTTGCTGataa        <  2:674789/140‑1 (MQ=255)                                                                                                                                tGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGTTGCTCACGCAGGGCGTCCTGTACGCGGGCTTCTTGCTGataat       >  2:503780/1‑140 (MQ=255)                                                                                                                                tGCGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGTTGCTCACGCAGGGCGTCCTGTACGCGGGCTTCTTGCTGataat       >  2:563616/1‑140 (MQ=255)                                                                                                                                  cGGCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGTTGCTCACGCAGGGCGTCCTGTACGCGGGCTTCTTGCTGAtaataa     >  1:314450/1‑140 (MQ=255)                                                                                                                                   ggCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGTTGCTCACGCAGGGCGTCCTGTACGCGGGCTTCTTGCTGATAATAAc    <  1:503780/140‑1 (MQ=255)                                                                                                                                    gCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGTTGCTCACGCAGGGCGTCCTGTACGCGGGCTTCTTGCt             <  2:88873/130‑1 (MQ=255)                                                                                                                                    gCCTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGTTGCTCACGCAGGGCGTCCTGTACGCGGGCTTCTTGCt             >  1:88873/1‑130 (MQ=255)                                                                                                                                     ccTGCACTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGTTGCTCACGCAGGGCGTCCTGTACGCGGGCTTCTTGCTGATAATAACGa  >  1:1324534/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       GTCCGTTGGCGGATTCCAGCGCCTGACAAAAAATGTTGTACAACTCGCTTTCGCCCGGTGCGCTGGTGCGATCCATATAGAGCATTTCAGTGCGGAACAAACCAACGCCTTCCGCACCATTGCCGAATGCGGCCTGCGCTTCCACGGAGTGAGCGATGTTAGCGGCAATTTCAATGCGGATACCGTCAGCGGTACGGGCTTGTTGGGTCAGCCAGACACGCTGTTGCTCACGCAGGGCGTCCTGTACGCGGGCTTCTTGCTGATAATAACGA  >  NC_000913/4140907‑4141178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑