Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,157,589	T→C	V247A (GTG→GCG)	argH →	argininosuccinate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,157,589	0	T	C	100.0%	225.7 / NA	62	V247A (GTG→GCG)	argH	argininosuccinate lyase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/32);  total (30/32)

GAACGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGTGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGCGAAGCGGGGTTTGTGGAGCTTTCTGACCGCGTGACTTCCGGTTCATCATTAATGCCGCAGAAGAAAAACCCGGATGCGCTGGAGCTGATT  >  NC_000913/4157454‑4157725                                                                                                                                        |                                                                                                                                         gAACGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCAt                                                                                                                                      <  2:648109/140‑1 (MQ=255)    cGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTg                                                                                                                                   <  1:592463/140‑1 (MQ=255)       ccTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTcg                                                                                                                                >  2:991669/1‑140 (MQ=255)       ccTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTcg                                                                                                                                >  2:582447/1‑140 (MQ=255)         tATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTcgcg                                                                                                                              <  1:991669/140‑1 (MQ=255)         tATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTcgcg                                                                                                                              <  2:1290187/140‑1 (MQ=255)            gAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGttt                                                                                                                           <  2:1300972/140‑1 (MQ=255)                 cGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTg                                                                                                                      <  2:1073116/140‑1 (MQ=255)                 cGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTg                                                                                                                      >  1:1283800/1‑140 (MQ=255)                 cGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTATCGACAGCGTTTCTGACCGCGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCCTCTGTCGCGTTTTGCTg                                                                                                                      >  2:797974/1‑140 (MQ=255)                  gACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGa                                                                                                                     <  2:1115051/140‑1 (MQ=255)                   aCCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGaa                                                                                                                    >  1:1286432/1‑140 (MQ=255)                     cGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGa                                                                                                                  >  2:597745/1‑140 (MQ=255)                            aGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGAtt                                                                                                           <  2:1286432/140‑1 (MQ=255)                            aGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGAtt                                                                                                           <  1:801699/140‑1 (MQ=255)                              ttAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCGTCTGTCGCGTTTTGCTGAAGATCTGAtttt                                                                                                         >  2:834559/1‑140 (MQ=255)                              ttAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGAtttt                                                                                                         >  1:494519/1‑140 (MQ=255)                                 gCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTg                                                                                                                      >  1:83099/1‑124 (MQ=255)                                 gCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTg                                                                                                                      <  2:83099/124‑1 (MQ=255)                                        ggctggGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGAtt                                                                                                           >  2:381275/1‑128 (MQ=255)                                        ggctggGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGAtt                                                                                                           >  2:536263/1‑128 (MQ=255)                                        ggctggGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGAtt                                                                                                           <  1:381275/128‑1 (MQ=255)                                        ggctggGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGAtt                                                                                                           <  1:536263/128‑1 (MQ=255)                                        ggctggGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACAcc                                                                                               >  1:650641/1‑140 (MQ=255)                                        ggctggGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACAcc                                                                                               >  1:889919/1‑140 (MQ=255)                                        ggctggGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACAcc                                                                                               >  2:1247496/1‑140 (MQ=255)                                        ggctggGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACAcc                                                                                               >  1:992274/1‑140 (MQ=255)                                         gctggGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCg                                                                                              <  1:1247496/140‑1 (MQ=255)                                                tttGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGCGAAGc                                                                                       >  1:920471/1‑140 (MQ=255)                                                tttGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGCGAAGc                                                                                       <  1:751298/140‑1 (MQ=255)                                                   gCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGCGAAGCggg                                                                                    <  2:373473/140‑1 (MQ=255)                                                     ttCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGCGAAGCGGGGt                                                                                  <  2:650641/140‑1 (MQ=255)                                                       cGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGc                                                                                            <  2:779426/128‑1 (MQ=255)                                                       cGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGc                                                                                            >  1:779426/1‑128 (MQ=255)                                                       cGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGCGAAGCGGGGttt                                                                                <  2:920471/140‑1 (MQ=255)                                                       cGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGCGAAGCGGGGttt                                                                                >  2:77955/1‑140 (MQ=255)                                                           gACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGCGAAGCGGGGTTTGTgg                                                                            <  1:77955/140‑1 (MQ=255)                                                            aCCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGCGAAGCGGGGTTTGTGGa                                                                           >  2:960823/1‑140 (MQ=255)                                                                   aCAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGCGAAGCGGGGTTTGTGGAGCTTTCt                                                                    <  1:996452/140‑1 (MQ=255)                                                                      gTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGCGAAGCGGGGTTTGTGGAGCTTTCTGAc                                                                 <  2:1108680/140‑1 (MQ=255)                                                                        ctcGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGCGAAGCGGGGTTTGTGGAGCTTTCTGACcg                                                               >  2:479086/1‑140 (MQ=255)                                                                         tcGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGCGAAGCGGGGTTTGTGGAGCTTTCTGACcgc                                                              >  1:1201440/1‑140 (MQ=255)                                                                         tcGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGCGAAGCGGGGTTTGTGGAGCTTTCTGACcgc                                                              <  2:1201440/140‑1 (MQ=255)                                                                            aCAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGCGAAGCGGGGTTTGTGGAGCTTTCTGACCGCGTg                                                           <  1:834559/140‑1 (MQ=255)                                                                                cGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGCGAAGCGGGGTTTGTGGAGCTTTCTGACCGCGTGACtt                                                       >  2:1112315/1‑140 (MQ=255)                                                                                cGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGCGAAGCGGGGTTTGTGGAGCTTTCTGACCGCGTGACtt                                                       >  2:692607/1‑140 (MQ=255)                                                                                 gTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGCGAAGCGGGGTTTGTGGAGCTTTCTGACCGCGTGACTTc                                                      <  1:960823/140‑1 (MQ=255)                                                                                       gACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGCGAAGCGGGGTTTGTGGAGCTTTCTGACCGCGTGACTTCCGGTtc                                                <  1:272214/140‑1 (MQ=255)                                                                                       gACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGCGAAGCGGGGTTTGTGGAGCTTTCTGACCGCGTGACTTCCGGTtc                                                <  1:597745/140‑1 (MQ=255)                                                                                                  tgtgTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGCGAAGCGGGGTTTGTGGAGCTTTCTGACCGCGTGACTTCCGGTtc                                                >  1:821742/1‑129 (MQ=255)                                                                                                  tgtgTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGCGAAGCGGGGTTTGTGGAGCTTTCTGACCGCGTGACTTCCGGTtc                                                <  2:821742/129‑1 (MQ=255)                                                                                                  tgtgTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGCGAAGCGGGGTTTGTGGAGCTTTCTGACCGCGTGACTTCCGGTTCATCATTAATGc                                     >  2:1258275/1‑140 (MQ=255)                                                                                                  tgtgTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGCGAAGCGGGGTTTGTGGAGCTTTCTGACCGCGTGACTTCCGGTTCATCATTAATGc                                     >  2:586084/1‑140 (MQ=255)                                                                                                      ttGGAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGCGAAGCGGGGTTTGTGGAGCTTTCTGACCGCGTGACTTCCGGTTCATCATTAATGCCGCa                                 >  1:419897/1‑140 (MQ=255)                                                                                                        ggAACTGCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGCGAAGCGGGGTTTGTGGAGCTTTCTGACCGCGTGACTTCCGGTTCATCATTAATGCCGCaga                               >  2:44180/1‑140 (MQ=255)                                                                                                              gCTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGCGAAGCGGGGTTTGTGGAGCTTTCTGACCGCGTGACTTCCGGTTCATCATTAATGCCGCAGAAGaaaa                         >  2:204486/1‑140 (MQ=255)                                                                                                               cTTTCTGCTGCCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGCGAAGCGGGGTTTGTGGAGCTTTCTGACCGCGTGACTTCCGGTTCATCATTAATGCCGCAGAAGaaaaa                        >  2:438991/1‑140 (MQ=255)                                                                                                                    tgctgcCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGCGAAGCGGGGTTTGTGGAGCTTTCTGACCGCGTGACTTCCGGTTCATCATTAATGCCGCAGAAGAAAAACCCgg                   <  1:355691/140‑1 (MQ=255)                                                                                                                     gctgcCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGCGAAGCGGGGTTTGTGGAGCTTTCTGACCGCGTGACTTCCGGTTCATCATTAATGCCGCAGAAGAAAAACCCGGa                  <  2:1259547/140‑1 (MQ=255)                                                                                                                     gctgcCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGCGAAGCGGGGTTTGTGGAGCTTTCTGACCGCGTGACTTCCGGTTCATCATTAATGCCGCAGAAGAAAAACCCGGa                  <  1:479086/140‑1 (MQ=255)                                                                                                                     gctgcCGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGCGAAGCGGGGTTTGTGGAGCTTTCTGACCGCGTGACTTCCGGTTCATCATTAATGCCGCAGAAGAAAAACCCGGa                  <  2:992274/140‑1 (MQ=255)                                                                                                                         ccGCTATCGGCATGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGCGAAGCGGGGTTTGTGGAGCTTTCTGACCGCGTGACTTCCGGTTCATCATTAATGCCGCAGAAGAAAAACCCGGATgcg              >  1:584697/1‑140 (MQ=255)                                                                                                                                     tGGCGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGCGAAGCGGGGTTTGTGGAGCTTTCTGACCGCGTGACTTCCGGTTCATCATTAATGCCGCAGAAGAAAAACCCGGATGCGCTGGAGCTGAtt  <  2:1283800/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         GAACGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGTGACCATGTGTTGGAACTGCTTTCTGCTGCCGCTATCGGCATGGTGCATCTGTCGCGTTTTGCTGAAGATCTGATTTTCTTTAACACCGGCGAAGCGGGGTTTGTGGAGCTTTCTGACCGCGTGACTTCCGGTTCATCATTAATGCCGCAGAAGAAAAACCCGGATGCGCTGGAGCTGATT  >  NC_000913/4157454‑4157725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑