Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,243,814	A→G	L421P (CTG→CCG)	malF ←	maltose ABC transporter membrane subunit MalF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,243,814	0	A	G	100.0%	300.2 / NA	82	L421P (CTG→CCG)	malF	maltose ABC transporter membrane subunit MalF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/42);  total (40/42)

GCAGGTCGGTATAACCGGCTGGCGTGGTCGTGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATT  >  NC_000913/4243677‑4243950                                                                                                                                          |                                                                                                                                         gCAGGTCGGTATAACCGGCTGGCGTGGTCGTGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCagcggc                                                                                                                                        >  1:458666/1‑140 (MQ=255)   aGGTCGGTATAACCGGCTGGCGTGGTCGTGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCATGAAGTGGTTAAAGTTTAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCAGTTTAATCAgcggcgg                                                                                                                                      >  2:1222792/1‑140 (MQ=255)   aGGTCGGTATAACCGGCTGGCGTGGTCGTGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAgcggcgg                                                                                                                                      >  1:1211479/1‑140 (MQ=255)        ggTATAACCGGCTGGCGTGGTCGTGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAgcggcggcagcg                                                                                                                                 <  2:894477/140‑1 (MQ=255)        ggTATAACCGGCTGGCGTGGTCGTGCCAAGACGATCC‑GGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGt                                                                                                                                <  2:295602/140‑1 (MQ=255)         gTATAACCGGCTGGCGTGGTCGTGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGt                                                                                                                                <  2:192213/140‑1 (MQ=255)         gTATAACCGGCTGGCGTGGTCGTGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGt                                                                                                                                <  2:583051/140‑1 (MQ=255)               ccGGCTGGCGTGGTCGTGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCtt                                                                                                                          >  1:1327716/1‑140 (MQ=255)               ccGGCTGGCGTGGTCGTGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCtt                                                                                                                          >  1:740583/1‑140 (MQ=255)                   cTGGCGTGGTCGTGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAaag                                                                                                                      >  1:1343627/1‑140 (MQ=255)                    tGGCGTGGTCGTGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAgcggcggcagcg                                                                                                                                 <  2:1129825/128‑1 (MQ=255)                    tGGCGTGGTCGTGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAgcggcggcagcg                                                                                                                                 >  1:1129825/1‑128 (MQ=255)                    tGGCGTGGTCGTGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAgcggcg                                                                                                                                       <  2:694259/122‑1 (MQ=255)                    tGGCGTGGTCGTGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAgcggcg                                                                                                                                       >  1:694259/1‑122 (MQ=255)                    tGGCGTGGTCGTGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAaaga                                                                                                                     >  1:749499/1‑140 (MQ=255)                             cGTGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGa                                                                                                            <  2:749499/140‑1 (MQ=255)                                     gACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCt                                                                                                    <  2:356329/140‑1 (MQ=255)                                        gATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTgcg                                                                                                 <  1:729655/140‑1 (MQ=255)                                        gATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTgcg                                                                                                 <  2:278352/140‑1 (MQ=255)                                          tCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGcc                                                                                               <  2:985718/140‑1 (MQ=255)                                          tCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGcc                                                                                               >  1:243787/1‑140 (MQ=255)                                          tCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGcc                                                                                               >  1:319127/1‑140 (MQ=255)                                          tCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGcc                                                                                               >  1:985718/1‑140 (MQ=255)                                               gccgccGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGccatcca                                                                                          <  2:1343627/140‑1 (MQ=255)                                               gccgccGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGccatcca                                                                                          >  1:785379/1‑140 (MQ=255)                                                    cgTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGccatcca                                                                                          >  1:1065757/1‑135 (MQ=255)                                                    cgTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGccatcca                                                                                          <  2:1065757/135‑1 (MQ=255)                                                         gTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGc                                                                                <  2:130423/140‑1 (MQ=255)                                                          ttAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCt                                                                               <  1:821630/140‑1 (MQ=255)                                                              cAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCAt                                                                           >  2:185847/1‑140 (MQ=255)                                                                gTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATAc                                                                         >  1:8907/1‑140 (MQ=255)                                                                   gAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACaaa                                                                      >  2:81161/1‑140 (MQ=255)                                                                   gAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACaaa                                                                      <  2:319127/140‑1 (MQ=255)                                                                        aGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGGAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGc                                                                                <  2:854385/125‑1 (MQ=255)                                                                        aGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGGAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGc                                                                                >  1:854385/1‑125 (MQ=255)                                                                         gCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTcc                                                                >  1:1277442/1‑140 (MQ=255)                                                                           aCGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCgg                                                              >  1:688735/1‑140 (MQ=255)                                                                             gAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGaa                                                            <  2:814058/140‑1 (MQ=255)                                                                               aGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGaa                                                            <  2:1342247/138‑1 (MQ=255)                                                                               aGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGaa                                                            >  1:1342247/1‑138 (MQ=255)                                                                                 ttgttAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGc                                                        <  2:614863/140‑1 (MQ=255)                                                                                  tgttAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCt                                                       <  2:740583/140‑1 (MQ=255)                                                                                  tgttAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCt                                                       <  2:1327716/140‑1 (MQ=255)                                                                                    ttaaagttaaagGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCttt                                                     >  1:1198173/1‑140 (MQ=255)                                                                                         gttaaagGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCa                                                >  2:800837/1‑140 (MQ=255)                                                                                         gttaaagGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCa                                                <  1:296867/140‑1 (MQ=255)                                                                                         gttaaagGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCa                                                >  2:782702/1‑140 (MQ=255)                                                                                         gttaaagGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCa                                                >  1:746968/1‑140 (MQ=255)                                                                                         gttaaagGCGAAGCTGGCGATCATAAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCt                                                       <  1:1075569/133‑1 (MQ=255)                                                                                         gttaaagGCGAAGCTGGCGATCATAAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCt                                                       >  2:1075569/1‑133 (MQ=255)                                                                                           taaagGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAg                                              >  1:205089/1‑140 (MQ=255)                                                                                           taaagGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAg                                              >  2:1264440/1‑140 (MQ=255)                                                                                           taaagGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAg                                              >  1:508287/1‑140 (MQ=255)                                                                                            aaagGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGc                                             >  1:1005921/1‑140 (MQ=255)                                                                                            aaagGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGc                                             >  1:178980/1‑140 (MQ=255)                                                                                            aaagGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGc                                             <  2:688735/140‑1 (MQ=255)                                                                                             aagGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGcc                                            >  1:936591/1‑140 (MQ=255)                                                                                              agGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGccc                                           <  1:30110/140‑1 (MQ=255)                                                                                                 cGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATg                                        <  1:1222792/140‑1 (MQ=255)                                                                                                   aaGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCa                                      >  1:1356924/1‑140 (MQ=255)                                                                                                   aaGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCa                                      >  2:1325375/1‑140 (MQ=255)                                                                                                   aaGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCa                                      <  2:178980/140‑1 (MQ=255)                                                                                                   aaGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCa                                      <  2:722563/140‑1 (MQ=255)                                                                                                   aaGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCa                                      >  1:1285909/1‑140 (MQ=255)                                                                                                    aGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCag                                     >  2:1058677/1‑140 (MQ=255)                                                                                                    aGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCag                                     >  1:1179812/1‑140 (MQ=255)                                                                                                     gCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCaga                                    <  2:360043/140‑1 (MQ=255)                                                                                                     gCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCaga                                    <  2:1179812/140‑1 (MQ=255)                                                                                                       tGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAgg                                  >  2:882151/1‑140 (MQ=255)                                                                                                        ggCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGa                                 <  1:1058677/140‑1 (MQ=255)                                                                                                        ggCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGa                                 <  2:1285909/140‑1 (MQ=255)                                                                                                        ggCGATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGa                                 <  2:508287/140‑1 (MQ=255)                                                                                                           gATCATCAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGatca                              <  2:755638/140‑1 (MQ=255)                                                                                                            atcatcAGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGatcat                             <  2:212008/140‑1 (MQ=255)                                                                                                             tcatcaGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGatcatc                            <  2:8907/140‑1 (MQ=255)                                                                                                               atcaGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATcatcat                          <  2:936591/140‑1 (MQ=255)                                                                                                                tcaGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATg                         <  1:800837/140‑1 (MQ=255)                                                                                                                 caGCGGCGTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGt                        >  2:413945/1‑140 (MQ=255)                                                                                                                    cggcgTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACg                     <  1:882151/140‑1 (MQ=255)                                                                                                                       cgTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGAt                  >  2:1229786/1‑140 (MQ=255)                                                                                                                       cgTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGAt                  >  1:289706/1‑140 (MQ=255)                                                                                                                       cgTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGAt                  >  1:132719/1‑140 (MQ=255)                                                                                                                       cgTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGAt                  >  2:857344/1‑140 (MQ=255)                                                                                                                        gTCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATa                 <  2:289706/140‑1 (MQ=255)                                                                                                                         tCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATaa                <  1:1325375/140‑1 (MQ=255)                                                                                                                         tCAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATaa                >  1:681477/1‑140 (MQ=255)                                                                                                                          cAGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAAc               <  1:53485/140‑1 (MQ=255)                                                                                                                           aGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAAcc              <  1:1229786/140‑1 (MQ=255)                                                                                                                           aGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAAcc              <  1:1264440/140‑1 (MQ=255)                                                                                                                           aGCGGTTTAATCAGCGGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAAcc              >  1:837682/1‑140 (MQ=255)                                                                                                                                       agcggcGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTAtt  <  2:1356924/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         GCAGGTCGGTATAACCGGCTGGCGTGGTCGTGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCAGCGGTTTAATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATT  >  NC_000913/4243677‑4243950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑