Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,379,308	G→A	A20V (GCC→GTC)	frdD ←	fumarate reductase membrane protein FrdD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,379,308	0	G	A	100.0%	258.3 / NA	75	A20V (GCC→GTC)	frdD	fumarate reductase membrane protein FrdD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/47);  total (28/47)

CGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTACCAGTACAGGGCAACAAACAGGATTACGATGGTGGCAACCACAGTTACCGCCCAGAGACTTTTG  >  NC_000913/4379173‑4379443                                                                                                                                        |                                                                                                                                        cgACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCAGCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGAcccc                                                                                                                                     >  2:1266816/1‑140 (MQ=255)    ccAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGaa                                                                                                                                  <  1:714276/140‑1 (MQ=255)    ccAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGaa                                                                                                                                  >  2:228677/1‑140 (MQ=255)        tGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAgg                                                                                                                              <  2:1249614/140‑1 (MQ=255)           aGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGccc                                                                                                                           <  2:1110549/140‑1 (MQ=255)           aGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGccc                                                                                                                           <  1:740202/140‑1 (MQ=255)                 gcgcGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAAt                                                                                                                     <  2:475503/140‑1 (MQ=255)                  cgcgAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATa                                                                                                                    >  1:574796/1‑140 (MQ=255)                  cgcgAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATa                                                                                                                    >  1:283016/1‑140 (MQ=255)                      aaCGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCgg                                                                                                                <  1:1085468/140‑1 (MQ=255)                            aGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTc                                                                                                          >  1:679016/1‑140 (MQ=255)                              aaCGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCGCAGAATACCGGTTCGTCAg                                                                                                        >  1:551600/1‑140 (MQ=255)                              aaCGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAg                                                                                                        <  2:813920/140‑1 (MQ=255)                                cgcgCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGaa                                                                                                      <  2:1362537/140‑1 (MQ=255)                                   gcTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGc                                                                                                   >  1:1211048/1‑140 (MQ=255)                                     tCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCtt                                                                                                 >  1:304865/1‑140 (MQ=255)                                          gCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCtttggat                                                                                            <  1:778310/140‑1 (MQ=255)                                          gCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCtttggat                                                                                            <  2:574796/140‑1 (MQ=255)                                          gCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCtttggat                                                                                            <  1:1326959/140‑1 (MQ=255)                                          gCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCtttggat                                                                                            <  1:453284/140‑1 (MQ=255)                                             cAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCtttggatttg                                                                                         >  2:276603/1‑140 (MQ=255)                                               gcgcATCACCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCtttggatttgga                                                                                       <  1:276603/140‑1 (MQ=255)                                                   aTCACCCGGAAACAGCCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTaa                                                                                   <  1:412742/140‑1 (MQ=255)                                                   aTCACCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTaa                                                                                   <  1:867035/140‑1 (MQ=255)                                                   aTCACCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTaa                                                                                   >  1:180187/1‑140 (MQ=255)                                                    tCACCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAat                                                                                  <  1:145587/140‑1 (MQ=255)                                                     cACCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAatc                                                                                 >  1:1215335/1‑140 (MQ=255)                                                           gAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCa                                                                           <  1:228677/140‑1 (MQ=255)                                                           gAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCa                                                                           <  2:869459/140‑1 (MQ=255)                                                           gAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCa                                                                           <  1:402694/140‑1 (MQ=255)                                                             aacaacCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAgg                                                                         <  1:1266816/140‑1 (MQ=255)                                                                aacCCCAGTGGCAGCAGCATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCTGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTc                                                                      <  2:1215335/140‑1 (MQ=255)                                                                aacCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTc                                                                      <  1:358321/140‑1 (MQ=255)                                                                aacCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTc                                                                      <  1:1218426/140‑1 (MQ=255)                                                                aacCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTc                                                                      <  1:740813/140‑1 (MQ=255)                                                                aacCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTc                                                                      <  2:304865/140‑1 (MQ=255)                                                                aacCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTc                                                                      <  2:317864/140‑1 (MQ=255)                                                                aacCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTc                                                                      <  1:220012/140‑1 (MQ=255)                                                                aacCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTc                                                                      <  2:952979/140‑1 (MQ=255)                                                                aacCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTc                                                                      <  2:1016044/140‑1 (MQ=255)                                                                aacCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTc                                                                      <  2:679016/140‑1 (MQ=255)                                                                aacCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCCGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTc                                                                      <  2:494870/140‑1 (MQ=255)                                                                  ccccAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCt                                                                    >  2:194728/1‑140 (MQ=255)                                                                  ccccAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAAAACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCt                                                                    >  2:1023770/1‑140 (MQ=255)                                                                     cAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTc                                                                      >  2:287874/1‑135 (MQ=255)                                                                     cAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTc                                                                      <  1:287874/135‑1 (MQ=255)                                                                     cAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTAc                                                                 >  1:41423/1‑140 (MQ=255)                                                                     cAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTAc                                                                 <  1:1023770/140‑1 (MQ=255)                                                                      aGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTAcc                                                                >  2:587052/1‑140 (MQ=255)                                                                          gcagcaGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTACCAGTa                                                            <  2:1271161/140‑1 (MQ=255)                                                                          gcagcaGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTACCAGTa                                                            <  1:194728/140‑1 (MQ=255)                                                                                  aTACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTACCAGTACAGGGcaa                                                    >  2:1237560/1‑140 (MQ=255)                                                                                   tACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTACCAGTACAGGGcaac                                                   <  1:16481/140‑1 (MQ=255)                                                                                      ccaccaGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTACCAGTACAGGGCaacaaa                                                <  1:829004/140‑1 (MQ=255)                                                                                        accaGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTACCAGTACAggg                                                       <  1:93067/131‑1 (MQ=255)                                                                                        accaGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTACCAGTACAggg                                                       >  2:93067/1‑131 (MQ=255)                                                                                           agcagGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTACCAGTACAGGGCAACAAACAGGa                                           >  2:1274055/1‑140 (MQ=255)                                                                                                   catcaCCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTACCAGTACAGGGCAACAAACAGGATTACGAtg                                   <  2:1140680/140‑1 (MQ=255)                                                                                                   catcaCCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTACCAGTACAGGGCAACAAACAGGATTACGAtg                                   <  1:1297007/140‑1 (MQ=255)                                                                                                    atcaCCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTACCAGTACAGGGCAACAAACAGGATTACGaggt                                  >  1:1064493/1‑137 (MQ=255)                                                                                                    atcaCCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTACCAGTACAGGGCAACAAACAGGATTACGa                                     <  1:751065/137‑1 (MQ=255)                                                                                                    atcaCCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTACCAGTACAGGGCAACAAACAGGATTACGa                                     >  2:751065/1‑137 (MQ=255)                                                                                                    atcaCCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTACCAGTACAGGGCAACAAACAGGATTACGAtgg                                  >  2:292336/1‑140 (MQ=255)                                                                                                     tcaCCGGCGCAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTACCAGTACAGGGCAACAAACAGGATTACGAtggt                                 <  2:180187/140‑1 (MQ=255)                                                                                                           gcgcAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTACCAGTACAGGGCAACAAACAGGATTACGATGGTGGCAAc                           >  1:524360/1‑140 (MQ=255)                                                                                                           gcgcAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTACCAGTACAGGGCAACAAACAGGATTACGATGGTGGCAAc                           <  2:733804/140‑1 (MQ=255)                                                                                                            cgcAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTACCAGTACAGGGCAACAAACAGGATTACGATGGTGGCAAcc                          >  2:416272/1‑140 (MQ=255)                                                                                                              cAATGATGGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTACCAGTACAGGGCAACAAACAGGATTACGATGGTGGCAACcac                        <  1:416272/140‑1 (MQ=255)                                                                                                                   atgGCGCTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTACCAGTACAGGGCAACAAACAGGATTACGATGGTGGCAACCACAGTTa                   <  1:292336/140‑1 (MQ=255)                                                                                                                      gcgcTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTACCAGTACAGGGCAACAAACAGGATTACGATGGTGGCAACCACAGTTACCg                >  2:1093245/1‑140 (MQ=255)                                                                                                                      gcgcTCCACATACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTACCAGTACAGGGCAACAAACAGGATTACGATGGTGGCAACCACAGTTACCg                >  1:599618/1‑140 (MQ=255)                                                                                                                             acaTACCACCGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTACCAGTACAGGGCAACAAACAGGATTACGATGGTGGCAACCACAGTTACCGCCCagag         <  2:1211048/140‑1 (MQ=255)                                                                                                                                  ccaccGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTACCAGTACAGGGCAACAAACAGGATTACGATGGTGGCAACCACAGTTACCGCCCAGAGACttt    <  1:1093245/140‑1 (MQ=255)                                                                                                                                    accGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTACCAGTACAGGGCAACAAACAGGATTACGATGGTGGCAACCACAGTTACCGCCCAGAGACTTTTg  >  1:228146/1‑140 (MQ=255)                                                                                                                                    accGACCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTACCAGTACAGGGCAACAAACAGGATTACGATGGTGGCAACCACAGTTACCGCCCAGAGACTTTTg  >  1:1368296/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        CGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTACCAGTACAGGGCAACAAACAGGATTACGATGGTGGCAACCACAGTTACCGCCCAGAGACTTTTG  >  NC_000913/4379173‑4379443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑