Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,380,030	C→T	G163D (GGC→GAC)	frdB ←	fumarate reductase iron‑sulfur protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,380,030	0	C	T	100.0%	340.3 / NA	97	G163D (GGC→GAC)	frdB	fumarate reductase iron‑sulfur protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (43/54);  total (43/54)

CACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTA  >  NC_000913/4379894‑4380160                                                                                                                                         |                                                                                                                                   cACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTACCGTGGTCACGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCaa                                                                                                                                 <  1:718283/140‑1 (MQ=255)   cGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAAc                                                                                                                               >  2:934947/1‑140 (MQ=255)        gTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCgg                                                                                                                          <  1:1021282/140‑1 (MQ=255)             gCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTTCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCGGGACCGATGATCTCTGGGTTCAGGTCAAACTGCGGGCAcg                                                                                                                     <  2:645827/140‑1 (MQ=255)              cTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCAcgc                                                                                                                    >  2:621959/1‑140 (MQ=255)                  aTACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACgcggcg                                                                                                                >  2:325363/1‑140 (MQ=255)                   tACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGt                                                                                                               >  2:809834/1‑140 (MQ=255)                    aCGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTa                                                                                                              <  1:621959/140‑1 (MQ=255)                       ccGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGca                                                                                                           <  1:1183442/140‑1 (MQ=255)                       ccGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGca                                                                                                           <  1:214785/140‑1 (MQ=255)                        cGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGcac                                                                                                          <  1:925049/140‑1 (MQ=255)                        cGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGcac                                                                                                          >  2:925049/1‑140 (MQ=255)                        cGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGcac                                                                                                          >  2:107774/1‑140 (MQ=255)                          ttCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACaa                                                                                                        >  1:1035507/1‑140 (MQ=255)                           tCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACaaa                                                                                                       >  2:164359/1‑140 (MQ=255)                           tCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACaaa                                                                                                       >  1:207132/1‑140 (MQ=255)                             tGGCTGTTCAACTGCGCCATACGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGGGGCGTAGCACAAAcc                                                                                                     <  1:809834/140‑1 (MQ=255)                             tGGCTGTTCAACTGCGCCATACGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAAcc                                                                                                     <  1:566132/140‑1 (MQ=255)                              ggCTGTTCAACTGCGCCATACGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACca                                                                                                    <  2:1035507/140‑1 (MQ=255)                                  gTTCAACTGCGCCATACGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGt                                                                                                >  1:676323/1‑140 (MQ=255)                                    tCAACTGCGCCATACGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAAcc                                                                                                     >  2:706005/1‑133 (MQ=255)                                    tCAACTGCGCCATACGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAAcc                                                                                                     <  1:706005/133‑1 (MQ=255)                                     cAACTGCGCCATACGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAAc                                                                                                      >  2:862480/1‑131 (MQ=255)                                     cAACTGCGCCATACGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAAc                                                                                                      <  1:862480/131‑1 (MQ=255)                                       aCTGCGCCATACGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATg                                                                                           >  2:724344/1‑140 (MQ=255)                                        cTGCGCCATACGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGc                                                                                          <  1:755214/140‑1 (MQ=255)                                           cgcCATACGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAAc                                                                                       <  2:110537/140‑1 (MQ=255)                                             ccATACGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCg                                                                                     >  2:9674/1‑140 (MQ=255)                                               aTACGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGga                                                                                   <  1:395443/140‑1 (MQ=255)                                               aTACGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGga                                                                                   <  2:676323/140‑1 (MQ=255)                                                tACGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGAt                                                                                            >  1:368752/1‑130 (MQ=255)                                                tACGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGAt                                                                                            <  2:368752/130‑1 (MQ=255)                                                 aCGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGgaga                                                                                 <  2:889447/140‑1 (MQ=255)                                                 aCGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGgaga                                                                                 <  2:404557/140‑1 (MQ=255)                                                 aCGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGgaga                                                                                 <  1:1203452/140‑1 (MQ=255)                                                 aCGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGgaga                                                                                 <  1:164359/140‑1 (MQ=255)                                                 aCGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGgaga                                                                                 <  1:934947/140‑1 (MQ=255)                                                  cGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGaa                                                                                <  2:125513/140‑1 (MQ=255)                                                  cGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGaa                                                                                <  2:162224/140‑1 (MQ=255)                                                          cttACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGAt                                                                        >  1:535664/1‑140 (MQ=255)                                                             aCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACt                                                                     >  1:1010478/1‑140 (MQ=255)                                                              ccGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACtt                                                                    >  2:8897/1‑140 (MQ=255)                                                               cGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTc                                                                   <  1:9674/140‑1 (MQ=255)                                                               cGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTc                                                                   >  1:842848/1‑140 (MQ=255)                                                                gTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCg                                                                  >  1:1201586/1‑140 (MQ=255)                                                                 tGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGgag                                                                                  >  1:138754/1‑123 (MQ=255)                                                                 tGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGgag                                                                                  <  2:138754/123‑1 (MQ=255)                                                                 tGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGc                                                                 >  2:673401/1‑140 (MQ=255)                                                                 tGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGc                                                                 >  1:219928/1‑140 (MQ=255)                                                                 tGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGc                                                                 >  2:610354/1‑140 (MQ=255)                                                                   gTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCa                                                               >  1:170368/1‑140 (MQ=255)                                                                   gTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCa                                                               >  1:388862/1‑140 (MQ=255)                                                                   gTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCC‑GACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGAt                                                                        <  1:1110244/130‑1 (MQ=255)                                                                   gTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCC‑GACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGAt                                                                        >  2:1110244/1‑130 (MQ=255)                                                                    tCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGAGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCAt                                                              >  2:1219126/1‑140 (MQ=255)                                                                       cgGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTg                                                           >  1:469653/1‑140 (MQ=255)                                                                            aTCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACGAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCg                                                      <  2:938616/140‑1 (MQ=255)                                                                              cTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCggg                                                    <  2:219928/140‑1 (MQ=255)                                                                                tCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGt                                                  <  2:388862/140‑1 (MQ=255)                                                                                tCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGt                                                  <  2:170368/140‑1 (MQ=255)                                                                                tCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGt                                                  <  1:1351794/140‑1 (MQ=255)                                                                                tCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGt                                                  <  1:756146/140‑1 (MQ=255)                                                                                  gTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCt                                                >  1:714042/1‑140 (MQ=255)                                                                                    tataACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTgg                                              >  2:905983/1‑140 (MQ=255)                                                                                     ataACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGa                                             <  1:305067/140‑1 (MQ=255)                                                                                     ataACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGa                                             <  1:459201/140‑1 (MQ=255)                                                                                     ataACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGa                                             <  1:8897/140‑1 (MQ=255)                                                                                     ataACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGa                                             <  1:164104/140‑1 (MQ=255)                                                                                       aaCGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTg                                               <  2:149810/136‑1 (MQ=255)                                                                                       aaCGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTg                                               >  1:149810/1‑136 (MQ=255)                                                                                       aaCGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATg                                           >  2:897030/1‑140 (MQ=255)                                                                                          gATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTa                                        <  2:516179/140‑1 (MQ=255)                                                                                          gATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTa                                        <  1:610354/140‑1 (MQ=255)                                                                                           aTGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTc                                                 <  1:1199118/130‑1 (MQ=255)                                                                                           aTGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTc                                                 >  2:1199118/1‑130 (MQ=255)                                                                                           aTGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATATTAg                                       >  2:1257649/1‑140 (MQ=255)                                                                                            tGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCggggg                                                  >  2:123980/1‑127 (MQ=255)                                                                                            tGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGt                                                  <  1:123980/128‑1 (MQ=255)                                                                                            tGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGt                                      >  1:1307639/1‑140 (MQ=255)                                                                                              cgcCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTAc                                    >  1:686872/1‑140 (MQ=255)                                                                                              cgcCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTAc                                    >  2:313149/1‑140 (MQ=255)                                                                                                ccAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTAccc                                  >  1:1039077/1‑140 (MQ=255)                                                                                                   gCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGa                               <  2:735116/140‑1 (MQ=255)                                                                                                       aaTGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCcg                           <  2:363086/140‑1 (MQ=255)                                                                                                       aaTGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCcg                           <  1:897030/140‑1 (MQ=255)                                                                                                       aaTGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCcg                           <  2:714042/140‑1 (MQ=255)                                                                                                         tGGCAGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCcgcg                         <  2:842848/140‑1 (MQ=255)                                                                                                             aGCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGc                     <  1:587873/140‑1 (MQ=255)                                                                                                              gCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCcg                           >  1:1079382/1‑133 (MQ=255)                                                                                                              gCCGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCcg                           <  2:1079382/133‑1 (MQ=255)                                                                                                               ccGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCgg                   <  1:325363/140‑1 (MQ=255)                                                                                                                cGGACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCggg                  >  1:133585/1‑140 (MQ=255)                                                                                                                 gaaCCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCACCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGa                 >  1:1352249/3‑140 (MQ=255)                                                                                                                  gACCGATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAg                <  2:469653/140‑1 (MQ=255)                                                                                                                      gATGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGTTGc            <  2:1307639/140‑1 (MQ=255)                                                                                                                        tGAACTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGTTGCCg          <  2:133585/140‑1 (MQ=255)                                                                                                                          aaCTCTGGGTTCAGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGTTGCCgat        <  1:1219126/140‑1 (MQ=255)                                                                                                                                      aGGTCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTa  <  1:137879/134‑1 (MQ=255)                                                                                                                                         |                                                                                                                                   CACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTA  >  NC_000913/4379894‑4380160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑