Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,445,095	C→T	G328G (GGC→GGT)	tamB →	translocation and assembly module subunit TamB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,445,095	0	C	T	100.0%	295.9 / NA	86	G328G (GGC→GGT)	tamB	translocation and assembly module subunit TamB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (44/42);  total (44/42)

AGCGGCACGGCGCAGCTGTCAGACAACTGGCCGGTGGACATCACTCTCAACAGTACACTGAACGTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGCGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGGCCGTTCACTGGTGAGAAGCAGTATCAGGCGG  >  NC_000913/4444961‑4445231                                                                                                                                       |                                                                                                                                         aGCGGCACGGCGCAGCTGTCAGACAACTGGCCGGTGGACATCACTCTCAACAGTACACTGAACGTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTaa                                                                                                                                     >  1:501585/1‑140 (MQ=255)  gCGGCACGGCGCAGCTGTCAGACAACTGGCCGGTGGACATCACTCTCAACAGTACACTGAACGTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAAt                                                                                                                                    >  1:383108/1‑140 (MQ=255)       aCGGCGCAGCTGTCAGACAACTGGCCGGTGGACATCACTCTCAACAGTACACTGAACGTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTtc                                                                                                                               <  1:384035/140‑1 (MQ=255)       aCGGCGCAGCTGTCAGACAACTGGCCGGTGGACATCACTCTCAACAGTACACTGAACGTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTtc                                                                                                                               <  1:379405/140‑1 (MQ=255)            gcAGCTGTCAGACAACTGGCCGGTGGACATCACTCTCAACAGTACACTGAACGTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTtccggtc                                                                                                                          >  1:441302/1‑140 (MQ=255)              aGCTGTCAGACAACTGGCCGGTGGACATCACTCTCAACAGTACACTGAACGTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTtccggtccg                                                                                                                        <  1:1201384/140‑1 (MQ=255)              aGCTGTCAGACAACTGGCCGGTGGACATCACTCTCAACAGTACACTGAACGTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTtccggtccg                                                                                                                        >  2:1201384/1‑140 (MQ=255)              aGCTGTCAGACAACTGGCCGGTGGACATCACTCTCAACAGTACACTGAACGTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTtccggtccg                                                                                                                        <  2:441302/140‑1 (MQ=255)              aGCTGTCAGACAACTGGCCGGTGGACATCACTCTCAACAGTACACTGAACGTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTtccggtccg                                                                                                                        <  1:183055/140‑1 (MQ=255)                cTGTCAGACAACTGGCCGGTGGACATCACTCTCAACAGTACACTGAACGTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTccggtccggt                                                                                                                      <  2:1245258/140‑1 (MQ=255)                    cAGACAACTGGCCGGTGGACATCACTCTCAACAGTACACTGAACGTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGat                                                                                                                  <  1:446102/140‑1 (MQ=255)                          aCTGGCCGGTGGACATCACTCTCAACAGTACACTGAACGTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGAt                                                                                                            >  2:994139/1‑140 (MQ=255)                             ggCCGGTGGACATCACTCTCAACAGTACACTGAACGTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTa                                                                                                         >  1:118677/1‑140 (MQ=255)                             ggCCGGTGGACATCACTCTCAACAGTACACTGAACGTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTa                                                                                                         >  1:99349/1‑140 (MQ=255)                             ggCCGGTGGACATCACTCTCAACAGTACACTGAACGTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTa                                                                                                         >  1:200794/1‑140 (MQ=255)                             ggCCGGTGGACATCACTCTCAACAGTACACTGAACGTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTa                                                                                                         >  2:405776/1‑140 (MQ=255)                             ggCCGGTGGACATCACTCTCAACAGTACACTGAACGTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTa                                                                                                         >  2:507025/1‑140 (MQ=255)                              gCCGGTGGACATCACTCTCAACAGTACACTGAACGTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTAc                                                                                                        >  1:456548/1‑140 (MQ=255)                                   tGGACATCACTCTCAACAGTACACTGAACGTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGcc                                                                                                   >  1:71464/1‑140 (MQ=255)                                      aCATCACTCTCAACAGTACACTGAACGTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGAt                                                                                                            >  2:1017545/1‑128 (MQ=255)                                      aCATCACTCTCAACAGTACACTGAACGTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGAt                                                                                                            <  1:1017545/128‑1 (MQ=255)                                        aTCACTCTCAACAGTACACTGAACGTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGAc                                                                                              <  1:405776/140‑1 (MQ=255)                                             tctcAACAGTACACTGAACGTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGAc                                                                                         >  2:744850/1‑140 (MQ=255)                                                  aCAGTACACTGAACGTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCg                                                                                    >  1:231197/1‑140 (MQ=255)                                                    aGTACACTGAACGTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGa                                                                                  <  2:200794/140‑1 (MQ=255)                                                         acTGAACGTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCg                                                                             >  1:185020/1‑140 (MQ=255)                                                         acTGAACGTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCg                                                                             >  2:59770/1‑140 (MQ=255)                                                           tGAACGTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGa                                                                           >  2:72108/1‑140 (MQ=255)                                                           tGAACGTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGa                                                                           >  1:106195/1‑140 (MQ=255)                                                           tGAACGTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGa                                                                           >  1:203614/1‑140 (MQ=255)                                                             aaCGTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGAtt                                                                         >  2:728472/1‑140 (MQ=255)                                                             aaCGTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGAtt                                                                         >  2:534805/1‑140 (MQ=255)                                                             aaCGTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGAtt                                                                         >  1:1353848/1‑140 (MQ=255)                                                             aaCGTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGAtt                                                                         >  2:1162152/1‑140 (MQ=255)                                                                gTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGAtt                                                                         <  1:1330845/137‑1 (MQ=255)                                                                gTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGAtt                                                                         >  2:1330845/1‑137 (MQ=255)                                                                gTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGcc                                                                      >  1:438145/1‑140 (MQ=255)                                                                 tGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCg                                                                     <  1:1162152/140‑1 (MQ=255)                                                                 tGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCg                                                                     <  2:203614/140‑1 (MQ=255)                                                                  ggAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGc                                                                    >  1:117414/1‑140 (MQ=255)                                                                   gAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCt                                                                   <  1:1121032/140‑1 (MQ=255)                                                                   gAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCt                                                                   <  2:545797/140‑1 (MQ=255)                                                                   gAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCt                                                                   <  2:438145/140‑1 (MQ=255)                                                                   gAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCt                                                                   <  1:900296/140‑1 (MQ=255)                                                                   gAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCt                                                                   <  1:994139/140‑1 (MQ=255)                                                                   gAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCt                                                                   <  1:744850/140‑1 (MQ=255)                                                                   gAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCt                                                                   <  1:685767/140‑1 (MQ=255)                                                                   gAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCt                                                                   >  1:462033/1‑140 (MQ=255)                                                                   gAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATAGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCt                                                                   >  2:1201246/1‑140 (MQ=255)                                                                   gAGCAGTTGAAAGGTGAAGACGTGAAGCTGACAATGGGCGGCGCGCTGCATGAACAGCTGGAGATTGCTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGCCGCGACTGGCGGAAGCCGGATTGCCGCt                                                                   <  2:807953/140‑1 (MQ=255)                                                                       cGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAAc                                                               >  2:248320/1‑140 (MQ=255)                                                                       cGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAAc                                                               >  1:763567/1‑140 (MQ=255)                                                                        gTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACg                                                              <  2:135804/140‑1 (MQ=255)                                                                        gTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACg                                                              <  2:117414/140‑1 (MQ=255)                                                                        gTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACg                                                              <  2:501585/140‑1 (MQ=255)                                                                        gTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACg                                                              <  2:462033/140‑1 (MQ=255)                                                                         ttGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGATATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGt                                                             >  1:593879/1‑140 (MQ=255)                                                                             aaGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGaa                                                         <  2:145578/140‑1 (MQ=255)                                                                                gTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTg                                                      >  1:757613/1‑140 (MQ=255)                                                                                   aaaaaGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCt                                                                   <  1:177330/124‑1 (MQ=255)                                                                                   aaaaaGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCt                                                                   >  2:177330/1‑124 (MQ=255)                                                                                   aaaaaGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGA‑TGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACa                                                  >  2:420182/1‑140 (MQ=255)                                                                                       aGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCa                                               <  2:231197/140‑1 (MQ=255)                                                                                          gAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAAc                                            >  2:316545/1‑140 (MQ=255)                                                                                          gAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAAc                                            >  2:339670/1‑140 (MQ=255)                                                                                          gAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAAc                                            >  1:915817/1‑140 (MQ=255)                                                                                          gAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATATTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGa                                                     <  1:419174/131‑1 (MQ=255)                                                                                          gAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATATTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGa                                                     >  2:419174/1‑131 (MQ=255)                                                                                           aaGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACa                                           >  1:187460/1‑140 (MQ=255)                                                                                            aGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAg                                          <  1:248320/140‑1 (MQ=255)                                                                                                  aaaTGGGCGGCGCGCTGCGCGAACAGCTGGATATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTAc                                    <  2:593879/140‑1 (MQ=255)                                                                                                   aaTGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACt                                   >  2:542741/1‑140 (MQ=255)                                                                                                     tGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTgg                                 <  2:185020/140‑1 (MQ=255)                                                                                                       ggcggcGCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGGcc                               <  2:456548/140‑1 (MQ=255)                                                                                                          ggcgCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGGCCGtt                            <  2:419633/140‑1 (MQ=255)                                                                                                          ggcgCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGGCCGtt                            <  2:106195/140‑1 (MQ=255)                                                                                                          ggcgCGCTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGGCCGtt                            <  2:763567/140‑1 (MQ=255)                                                                                                           gcgcgcTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGGCCGTTc                           >  2:594261/1‑140 (MQ=255)                                                                                                            cgcgcTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGGCCGTTCa                          <  1:316545/140‑1 (MQ=255)                                                                                                             gcgcTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGGCCGTTCAc                         <  1:826546/140‑1 (MQ=255)                                                                                                             gcgcTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGGCCGTTCAc                         <  1:72108/140‑1 (MQ=255)                                                                                                                cTGCGCGAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGGCCGTTCACTgg                      <  2:1017404/140‑1 (MQ=255)                                                                                                                     cgAACAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGGCCGTTCACTGGTgaga                 <  1:542741/140‑1 (MQ=255)                                                                                                                       aaCAGCTGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGGCCGTTCACTGGTGAGAAg               <  2:383108/140‑1 (MQ=255)                                                                                                                             tGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGGCCGTTCACTGGTGAGAAGCAGTAt         <  1:594261/140‑1 (MQ=255)                                                                                                                             tGGAGATTGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGGCCGTTCACTGGTGAGAAGCAGTAt         >  2:975613/1‑140 (MQ=255)                                                                                                                                    tGGTGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGGCCGTTCACTGGTGAGAAGCAGTATCAGGCgg  >  2:137734/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                         AGCGGCACGGCGCAGCTGTCAGACAACTGGCCGGTGGACATCACTCTCAACAGTACACTGAACGTGGAGCCGTTGAAAGGTGAAAAAGTGAAGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGCGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGGCCGTTCACTGGTGAGAAGCAGTATCAGGCGG  >  NC_000913/4444961‑4445231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑