Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,550,283	C→T	S338S (TCG→TCA)	gntP ←	fructuronate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,550,283	0	C	T	98.9%	291.6 / NA	88	S338S (TCG→TCA)	gntP	fructuronate transporter
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (42/44);  total (44/44)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCACCATTGCAATACTCTTCACCGCGCTTTCAAAAGCGT  >  NC_000913/4550147‑4550419                                                                                                                                         |                                                                                                                                         tCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTgag                                                                                                                                       <  2:1226066/140‑1 (MQ=255)           cAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGt                                                                                                                             <  1:1080405/140‑1 (MQ=255)             gCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTgg                                                                                                                           <  2:118896/140‑1 (MQ=255)               tGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGa                                                                                                                         >  2:974319/1‑140 (MQ=255)               tGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGa                                                                                                                         >  1:924215/1‑140 (MQ=255)               tGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGa                                                                                                                         >  1:44065/1‑140 (MQ=255)                  ccAGTTGCTGGACCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACAt                                                                                                                      >  2:1334376/1‑140 (MQ=255)                     gTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCaa                                                                                                                   <  1:237575/140‑1 (MQ=255)                      ttGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGAc                                                                                                                        <  2:977649/134‑1 (MQ=255)                      ttGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGAc                                                                                                                        >  1:977649/1‑134 (MQ=255)                      ttGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGAc                                                                                                                        >  1:520737/1‑134 (MQ=255)                      ttGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGAc                                                                                                                        <  2:520737/134‑1 (MQ=255)                      ttGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAAc                                                                                                                  >  2:474486/1‑140 (MQ=255)                      ttGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAAc                                                                                                                  <  2:1060447/140‑1 (MQ=255)                        gCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACAt                                                                                                                >  1:730376/1‑140 (MQ=255)                            gATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCg                                                                                                            <  1:262241/140‑1 (MQ=255)                            gATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCg                                                                                                            <  1:974319/140‑1 (MQ=255)                                tAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATgg                                                                                                        >  2:80936/1‑140 (MQ=255)                                      tGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGc                                                                                                  >  2:300686/1‑140 (MQ=255)                                      tGCAGCACTGATAATCCCGGCGGCGATCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGCGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGc                                                                                                  >  2:1227700/1‑140 (MQ=255)                                       gcagcaCTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCg                                                                                                            <  2:1169042/129‑1 (MQ=255)                                       gcagcaCTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCg                                                                                                            >  1:1169042/1‑129 (MQ=255)                                       gcagcaCTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGcc                                                                                                 >  2:571887/1‑140 (MQ=255)                                        cagcaCTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTATCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCa                                                                                                >  1:190399/1‑140 (MQ=255)                                        cagcaCTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGcc                                                                                                 >  1:239714/1‑139 (MQ=255)                                        cagcaCTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGcc                                                                                                 <  2:239714/139‑1 (MQ=255)                                        cagcaCTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCa                                                                                                >  1:248147/1‑140 (MQ=255)                                        cagcaCTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCa                                                                                                >  2:108182/1‑140 (MQ=255)                                        cagcaCTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCa                                                                                                >  2:455333/1‑140 (MQ=255)                                        cagcaCTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCa                                                                                                >  2:70089/1‑140 (MQ=255)                                        cagcaCTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCa                                                                                                >  2:285818/1‑140 (MQ=255)                                        cagcaCTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCa                                                                                                >  2:333447/1‑140 (MQ=255)                                            aCTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGc                                                                                            <  2:680849/140‑1 (MQ=255)                                            aCTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGc                                                                                            <  1:1294487/140‑1 (MQ=255)                                             cTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTATCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGcc                                                                                           <  2:190399/140‑1 (MQ=255)                                             cTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGcc                                                                                           >  1:1323823/1‑140 (MQ=255)                                                aTAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGgt                                                                                        >  1:1140035/1‑140 (MQ=255)                                                  aaTCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGgtgt                                                                                      <  2:39001/140‑1 (MQ=255)                                                      ccGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCgat                                                                                  <  2:694537/140‑1 (MQ=255)                                                       cggcggcggTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCgatg                                                                                 <  1:231433/140‑1 (MQ=255)                                                        ggcggcggTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGGGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCgatga                                                                                >  2:901972/1‑140 (MQ=255)                                                          cggcggTCATCTCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGatgatg                                                                              >  1:299150/1‑140 (MQ=255)                                                          cggcggTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCg                                                                                          <  1:245349/128‑1 (MQ=255)                                                          cggcggTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCg                                                                                          <  1:171726/128‑1 (MQ=255)                                                          cggcggTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCg                                                                                          >  2:171726/1‑128 (MQ=255)                                                          cggcggTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCg                                                                                          >  2:245349/1‑128 (MQ=255)                                                            gcggTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGt                                                                            <  2:1099663/140‑1 (MQ=255)                                                                        gAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCAc                                                                <  1:926226/140‑1 (MQ=255)                                                                         aaaCGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACg                                                               >  2:642241/1‑140 (MQ=255)                                                                              acacCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCAcc                                                          <  2:1236731/140‑1 (MQ=255)                                                                                acCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCg                                                        <  1:474486/140‑1 (MQ=255)                                                                                  ccTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCa                                                      <  1:990374/140‑1 (MQ=255)                                                                                     gACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCg                                                   <  2:1140035/140‑1 (MQ=255)                                                                                           tCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCAc                                                           >  2:185464/1‑126 (MQ=255)                                                                                           tCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCAc                                                           <  1:185464/126‑1 (MQ=255)                                                                                           tCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATc                                             >  2:647919/1‑140 (MQ=255)                                                                                           tCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATc                                             >  1:1023647/1‑140 (MQ=255)                                                                                            cGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGCAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCa                                            >  1:697202/1‑140 (MQ=255)                                                                                                aGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAAt                                        >  2:697745/1‑140 (MQ=255)                                                                                                aGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAAt                                        <  2:299150/140‑1 (MQ=255)                                                                                                   cGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCAc                                     <  2:1008088/140‑1 (MQ=255)                                                                                                   cGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCAc                                     <  1:1195979/140‑1 (MQ=255)                                                                                                   cGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCAc                                     <  1:285818/140‑1 (MQ=255)                                                                                                   cGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCAc                                     <  1:333447/140‑1 (MQ=255)                                                                                                   cGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCAc                                     <  1:371618/140‑1 (MQ=255)                                                                                                   cGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCAc                                     <  1:554634/140‑1 (MQ=255)                                                                                                   cGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCAc                                     <  1:642241/140‑1 (MQ=255)                                                                                                   cGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCAc                                     <  1:70089/140‑1 (MQ=255)                                                                                                   cGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCAc                                     <  1:80936/140‑1 (MQ=255)                                                                                                   cGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCAc                                     <  1:901972/140‑1 (MQ=255)                                                                                                   cGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCAc                                     <  2:1023647/140‑1 (MQ=255)                                                                                                   cGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCAc                                     <  2:1323823/140‑1 (MQ=255)                                                                                                     aaTTAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCACCa                                   <  1:1334376/140‑1 (MQ=255)                                                                                                     aaTTAGCACAGTGATCAGCCATGCCATGATGCAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCACCa                                   <  2:697202/140‑1 (MQ=255)                                                                                                        tAGCACAGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCACCATTg                                <  2:730376/140‑1 (MQ=255)                                                                                                            acaGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCACCATTGCaa                             >  1:573446/1‑139 (MQ=255)                                                                                                            acaGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCACCATTGCaa                             <  2:573446/139‑1 (MQ=255)                                                                                                              aGTGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCACCATTGCAATAc                          <  1:300686/140‑1 (MQ=255)                                                                                                                tGATCAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCACCATTGCAATActc                        >  1:798578/1‑140 (MQ=255)                                                                                                                    cAGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCACCATTGCAATACTCTTCa                    >  1:1289925/1‑140 (MQ=255)                                                                                                                     aGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCACCATTGCAATACTCTTCAc                   >  1:793521/1‑140 (MQ=255)                                                                                                                     aGCCATGCCATGATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCACCATTGCAATACTCTTCAc                   >  1:185870/1‑140 (MQ=255)                                                                                                                        catgccatgATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATAGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCACCATTGCAATACTCTTCACcgc                >  1:183883/1‑140 (MQ=255)                                                                                                                          tgccatgATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCACCATTGCAATACTCTTCACCgcgc              >  1:1254970/1‑140 (MQ=255)                                                                                                                            ccatgATGTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCACCATTGCAATACTCTTCACCGCGCtt            >  1:967858/1‑140 (MQ=255)                                                                                                                               tgatgTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCACCATTGCAATACTCTTCACCGCGCTTTCa         >  1:1010342/1‑140 (MQ=255)                                                                                                                                 atgTAGGGTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCACCATTGCAATACTCTTCACCGCGCTTTCaaa       >  1:302606/1‑140 (MQ=255)                                                                                                                                      gggTGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCACCATTGCAATACTCTTCACCGCGCTTTCAAAAGCGt  >  1:1323519/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         TCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCACCATTGCAATACTCTTCACCGCGCTTTCAAAAGCGT  >  NC_000913/4550147‑4550419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑