Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,559,111	A→G	V139A (GTC→GCC)	iadA ←	isoaspartyl dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,559,111	0	A	G	100.0%	218.9 / NA	60	V139A (GTC→GCC)	iadA	isoaspartyl dipeptidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/23);  total (37/23)

TTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGACATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCG  >  NC_000913/4558988‑4559243                                                                                                                             |                                                                                                                                     ttGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGt                                                                                                                      <  1:374674/140‑1 (MQ=255)       aGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCAt                                                                                                                >  2:333375/1‑140 (MQ=255)        ggTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATc                                                                                                               <  1:504326/140‑1 (MQ=255)         gTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATcc                                                                                                              >  2:429017/1‑140 (MQ=255)         gTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATcc                                                                                                              >  1:847888/1‑140 (MQ=255)         gTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATcc                                                                                                              >  2:151358/1‑140 (MQ=255)                   tCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGa                                                                                                     >  2:257529/1‑140 (MQ=255)                   tCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGAt                                                                                                    >  2:314994/1‑140 (MQ=255)                   tCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGAt                                                                                                    >  2:607083/1‑140 (MQ=255)                   tCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGAt                                                                                                    >  2:137381/1‑140 (MQ=255)                   tCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGAt                                                                                                    >  1:554879/1‑140 (MQ=255)                    ccGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATg                                                                                                   >  1:428626/1‑140 (MQ=255)                     cGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGc                                                                                                  <  1:367817/140‑1 (MQ=255)                     cGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGc                                                                                                  <  1:607083/140‑1 (MQ=255)                     cGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGc                                                                                                  <  1:429017/140‑1 (MQ=255)                      ggTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGcc                                                                                                 >  2:1142708/1‑140 (MQ=255)                      ggTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGcc                                                                                                 <  1:137381/140‑1 (MQ=255)                       gTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCct                                                                                                >  2:867012/1‑140 (MQ=255)                       gTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCct                                                                                                >  2:768493/1‑140 (MQ=255)                               gAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCAtt                                                                                        <  1:333375/140‑1 (MQ=255)                                 aCGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGa                                                                                      <  1:854868/140‑1 (MQ=255)                                       aTCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCGGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCAc                                                                                <  1:245011/140‑1 (MQ=255)                                              aTGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCtt                                                                         >  2:111361/1‑140 (MQ=255)                                                 gcgcATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGc                                                                      >  2:307380/1‑140 (MQ=255)                                                 gcgcATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGc                                                                      >  1:336055/1‑140 (MQ=255)                                                  cgcATTTCACGCCAATCACACGATCGATAATCACCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCAc                                                                                <  1:484028/129‑1 (MQ=255)                                                  cgcATTTCACGCCAATCACACGATCGATAATCACCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCAc                                                                                >  2:484028/1‑129 (MQ=255)                                                   gcATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCg                                                                     <  1:257529/140‑1 (MQ=255)                                                      tttCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCa                                                                 >  2:466762/1‑140 (MQ=255)                                                       ttCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCGGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAg                                                                <  2:610314/140‑1 (MQ=255)                                                       ttCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAg                                                                <  2:834822/140‑1 (MQ=255)                                                             ccAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTc                                                          <  1:768493/140‑1 (MQ=255)                                                               aaTCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCg                                                        >  2:737225/1‑140 (MQ=255)                                                                     acGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGc                                                  <  2:1179965/140‑1 (MQ=255)                                                                      cgatcgatAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCg                                                 <  1:1142708/140‑1 (MQ=255)                                                                            atAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGAt                                           >  1:757242/1‑140 (MQ=255)                                                                            atAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGAt                                           >  2:445521/1‑140 (MQ=255)                                                                            atAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGAt                                           >  1:454673/1‑140 (MQ=255)                                                                               aTCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATaga                                        >  1:1234175/1‑140 (MQ=255)                                                                                 cGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGt                                      >  1:819780/1‑140 (MQ=255)                                                                                 cGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGt                                      <  2:819780/140‑1 (MQ=255)                                                                                 cGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGt                                      <  1:344863/140‑1 (MQ=255)                                                                                   ccACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCGAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGACAGATAGAGTCg                                    >  2:1200526/1‑140 (MQ=255)                                                                                   ccACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCg                                    >  2:945970/1‑140 (MQ=255)                                                                                   ccACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCg                                    >  2:740478/1‑140 (MQ=255)                                                                                   ccACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCg                                    >  1:580392/1‑140 (MQ=255)                                                                                   ccACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCg                                    >  1:479255/1‑140 (MQ=255)                                                                                   ccACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCg                                    >  1:1160996/1‑140 (MQ=255)                                                                                     aCGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGt                                  >  1:511420/1‑140 (MQ=255)                                                                                         cTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGccc                              <  2:580392/140‑1 (MQ=255)                                                                                                 aCGGAACCCGTAATGGTGCGGGAA‑GGGGCATGGTAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGacc                      <  1:350027/140‑1 (MQ=255)                                                                                                 aCGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGacc                      <  1:70840/140‑1 (MQ=255)                                                                                                  cGGAACCCGTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGacca                     >  2:912689/1‑140 (MQ=255)                                                                                                          gTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGaccaac                   <  2:1210542/134‑1 (MQ=255)                                                                                                          gTAATGGTGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGaccaac                   >  1:1210542/1‑134 (MQ=255)                                                                                                                 tGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACtgacgtgacg      >  2:398126/1‑140 (MQ=255)                                                                                                                 tGCGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACtgacgtgacg      >  1:88793/1‑140 (MQ=255)                                                                                                                   cGGGAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGcc    >  2:123869/1‑140 (MQ=255)                                                                                                                    gggAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCC‑TCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCg  <  1:151358/140‑1 (MQ=255)                                                                                                                     ggAA‑GGGGCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCg  >  1:702613/1‑140 (MQ=255)                                                                                                                             |                                                                                                                                     TTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAA‑GGGACATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCG  >  NC_000913/4558988‑4559243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑