Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,608,220	A→G	L12L (TTA→TTG)	rimI →	ribosomal‑protein‑S18‑alanine N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,608,220	0	A	G	100.0%	155.4 / NA	43	L12L (TTA→TTG)	rimI	ribosomal‑protein‑S18‑alanine N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/21);  total (22/21)

AACCGCTATCACTGGAAGGCGCTCAGGTGGCATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACG  >  NC_000913/4608083‑4608355                                                                                                                                          |                                                                                                                                        aaCCGCTATCACTGGAAGGCGCTCAGGTGGCATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGcc                                                                                                                                       >  1:1303791/1‑140 (MQ=255)  aCCGCTATCACTGGAAGGCGCTCAGGTGGCATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCcg                                                                                                                                      >  1:229737/1‑140 (MQ=255)     gCTATCACTGGAAGGCGCTCAGGTGGCATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCcggcg                                                                                                                                   <  1:1008557/140‑1 (MQ=255)     gCTATCACTGGAAGGCGCTCAGGTGGCATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCcggcg                                                                                                                                   <  2:1087025/140‑1 (MQ=255)     gCTATCACTGGAAGGCGCTCAGGTGGCATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCcggcg                                                                                                                                   <  1:545789/140‑1 (MQ=255)      cTATCACTGGAAGGCGCTCAGGTGGCATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCcggcgg                                                                                                                                  >  1:1288712/1‑140 (MQ=255)      cTATCACTGGAAGGCGCTCAGGTGGCATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCcggcgg                                                                                                                                  >  1:292317/1‑140 (MQ=255)          cACTGGAAGGCGCTCAGGTGGCATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTa                                                                                                                              <  2:229737/140‑1 (MQ=255)          cACTGGAAGGCGCTCAGGTGGCATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTa                                                                                                                              <  2:1288712/140‑1 (MQ=255)          cACTGGAAGGCGCTCAGGTGGCATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTa                                                                                                                              <  1:674123/140‑1 (MQ=255)                aaGGCGCTCAGGTGGCATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAGCACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACAt                                                                                                                        >  1:1185501/1‑140 (MQ=255)                    cgcTCAGGTGGCATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGaa                                                                                                                    >  2:577557/1‑140 (MQ=255)                     gcTCAGGTGGCATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGaac                                                                                                                   >  1:894813/1‑140 (MQ=255)                        cAGGTGGCATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGaacaac                                                                                                                <  2:1076213/140‑1 (MQ=255)                              gCATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGccc                                                                                                          >  2:436050/1‑140 (MQ=255)                               cATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCa                                                                                                         <  2:292317/140‑1 (MQ=255)                               cATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCa                                                                                                         <  2:1226666/140‑1 (MQ=255)                                 tCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACg                                                                                                       <  2:894813/140‑1 (MQ=255)                                   aCCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGcc                                                                                                     >  2:760710/1‑140 (MQ=255)                                           tCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTg                                                                                             >  1:1359670/1‑140 (MQ=255)                                            cACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTgg                                                                                            >  1:97187/1‑140 (MQ=255)                                             aCCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTcc                                                                                                <  1:1048975/135‑1 (MQ=255)                                             aCCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTcc                                                                                                >  2:1048975/1‑135 (MQ=255)                                                         gCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGt                                                                               >  2:1256120/1‑140 (MQ=255)                                                           aaaCCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCTCATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGttt                                                                             >  1:594225/1‑140 (MQ=255)                                                              cccAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGcc                                                                          <  2:1274289/140‑1 (MQ=255)                                                              cccAACGGCACGCGCCGCGTTATGGCAACAAACTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACACGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGcc                                                                          <  2:1205970/140‑1 (MQ=255)                                                                    ggCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAGCACGATTTCTTCCCTCGAAACGACTGCTTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAAc                                                                    <  2:1185501/140‑1 (MQ=255)                                                                        cgcgCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAACCAgg                                                                >  2:354676/1‑140 (MQ=255)                                                                               cgTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCCTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAACCAGGGCGAGCg                                                         <  2:948010/140‑1 (MQ=255)                                                                                     ggtaacaaATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTAtct                                                   <  1:577557/137‑1 (MQ=255)                                                                                      gCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTAtctc                                                  <  2:1359670/140‑1 (MQ=255)                                                                                         acaaATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAAc                                               >  1:1070291/1‑140 (MQ=255)                                                                                         acaaATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAAc                                               <  1:436050/140‑1 (MQ=255)                                                                                                       atGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAAGCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCa                                 >  2:939044/1‑140 (MQ=255)                                                                                                               gATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCa                         >  1:1042010/1‑140 (MQ=255)                                                                                                               gATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCa                         >  1:490641/1‑140 (MQ=255)                                                                                                                 tttCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCaaa                       >  1:829861/1‑140 (MQ=255)                                                                                                                   tcttcCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAAt                     <  2:1303791/140‑1 (MQ=255)                                                                                                                       cccTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATggcg                 >  2:516515/1‑140 (MQ=255)                                                                                                                       cccTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATggcg                 <  2:97187/140‑1 (MQ=255)                                                                                                                                   cTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAACCAGGGAGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGAtt     >  1:1111188/1‑140 (MQ=255)                                                                                                                                      aTTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACg  <  2:1215477/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        AACCGCTATCACTGGAAGGCGCTCAGGTGGCATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACG  >  NC_000913/4608083‑4608355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑