Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,609,962	C→T	Y183Y (TAC→TAT)	prfC →	peptide chain release factor RF3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,609,962	0	C	T	100.0%	240.6 / NA	68	Y183Y (TAC→TAT)	prfC	peptide chain release factor RF3
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/42);  total (26/42)

CTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTACCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCCACACCATTCAGGAAGTCCGCATTGTTAAAGGGCTGAATAACCCGGATCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGT  >  NC_000913/4609825‑4610097                                                                                                                                          |                                                                                                                                        cTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTatca                                                                                                                                       <  1:895587/140‑1 (MQ=255) cTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTatca                                                                                                                                       >  2:960394/1‑140 (MQ=255) cTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTatca                                                                                                                                       >  2:166787/1‑140 (MQ=255)    aCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCt                                                                                                                                    >  1:83757/1‑140 (MQ=255)    aCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCt                                                                                                                                    >  1:796853/1‑140 (MQ=255)         tATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTtata                                                                                                                               >  1:899261/1‑140 (MQ=255)         tATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTtata                                                                                                                               <  1:309943/140‑1 (MQ=255)         tATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTtata                                                                                                                               <  1:298433/140‑1 (MQ=255)           tGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATaaa                                                                                                                             >  2:1053798/1‑140 (MQ=255)              aCAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGAc                                                                                                                          <  1:756864/140‑1 (MQ=255)              aCAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGAc                                                                                                                          <  1:166787/140‑1 (MQ=255)               cAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACg                                                                                                                         <  1:391567/140‑1 (MQ=255)               cAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACg                                                                                                                         <  1:352989/140‑1 (MQ=255)                   cTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAAc                                                                                                                     <  1:726444/140‑1 (MQ=255)                   cTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAAc                                                                                                                     <  2:475358/140‑1 (MQ=255)                   cTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAAc                                                                                                                     >  1:475358/1‑140 (MQ=255)                            gATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTa                                                                                                            >  2:1279981/1‑140 (MQ=255)                            gATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTa                                                                                                            >  2:1332151/1‑140 (MQ=255)                             atatCCGCGACACGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTAt                                                                                                           >  2:574979/1‑140 (MQ=255)                                 ccGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCaga                                                                                                       >  1:1357354/1‑140 (MQ=255)                                  cgcgGCCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCTCCTTTATAAAGACGAAACCTATCTCTATCagag                                                                                                      <  2:646095/140‑1 (MQ=255)                                  cgcgACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCagag                                                                                                      <  1:1026508/140‑1 (MQ=255)                                  cgcgACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCagag                                                                                                      <  2:901554/140‑1 (MQ=255)                                  cgcgACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCagag                                                                                                      <  2:83757/140‑1 (MQ=255)                                  cgcgACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCagag                                                                                                      >  2:405670/1‑140 (MQ=255)                                      acacGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGt                                                                                                  <  1:574979/137‑1 (MQ=255)                                      aCCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGt                                                                                                  <  2:899261/140‑1 (MQ=255)                                       cccGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTa                                                                                                 >  2:76163/1‑140 (MQ=255)                                       cccGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTa                                                                                                 <  1:1053798/140‑1 (MQ=255)                                           aTGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAgg                                                                                             <  1:1332151/140‑1 (MQ=255)                                               aGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCagag                                                                                                      >  2:371556/1‑127 (MQ=255)                                               aGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCagag                                                                                                      <  1:371556/127‑1 (MQ=255)                                               aGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCcac                                                                                         <  1:106287/140‑1 (MQ=255)                                               aGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCcac                                                                                         <  1:635157/140‑1 (MQ=255)                                                   gctCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTGTCAGAGCGGTAAAGGCCACACCa                                                                                     <  2:489709/140‑1 (MQ=255)                                                      cGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCCacac                                                                                       >  2:765995/1‑135 (MQ=255)                                                      cGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCCacac                                                                                       <  1:765995/135‑1 (MQ=255)                                                         tGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCca                                                                                          >  1:91539/1‑129 (MQ=255)                                                         tGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCca                                                                                          <  2:91539/129‑1 (MQ=255)                                                         tGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCCACACCATTCAgg                                                                               <  2:1124260/140‑1 (MQ=255)                                                         tGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCCACACCATTCAgg                                                                               >  1:1285638/1‑140 (MQ=255)                                                             gTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCCACACCATTCAGGAAGt                                                                           <  2:266986/140‑1 (MQ=255)                                                                gagaACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCCACACCATTCAGGAAGTCCg                                                                        <  1:76163/140‑1 (MQ=255)                                                                gagaACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCCACACCATTCAGGAAGTCCg                                                                        <  2:1357354/140‑1 (MQ=255)                                                                gagaACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCCACACCATTCAGGAAGTCCg                                                                        <  1:1262185/140‑1 (MQ=255)                                                                 agaACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCCACACCATTCAGGAAGTCCGc                                                                       >  2:609754/1‑140 (MQ=255)                                                                 agaACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCCACACCATTCAGGAAGTCCGc                                                                       <  1:1064315/140‑1 (MQ=255)                                                                   aaCGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCCACACCATTCAGGAAGTCCGCAt                                                                     <  2:1285638/140‑1 (MQ=255)                                                                   aaCGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCCACACCATTCAGGAAGTCCGCAt                                                                     >  1:1161521/1‑140 (MQ=255)                                                                        gCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCCACACCATTCAGGAAGTCCGCATTGTTa                                                                >  1:458332/1‑140 (MQ=255)                                                                             aaaTCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCCACACCATTCAGGAAGTCCGCATTGTTAAAggg                                                           <  2:796853/140‑1 (MQ=255)                                                                                  ggCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCCACACCATTCAGGAAGTCCGCATTGTTa                                                                >  1:1347031/1‑130 (MQ=255)                                                                                  ggCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCCACACCATTCAGGAAGTCCGCATTGTTa                                                                <  2:1347031/130‑1 (MQ=255)                                                                                       tgCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCCACACCATTCAGGAAGTCCGCATTGTTAAAGGGCTGAATAAcc                                                 <  2:577925/140‑1 (MQ=255)                                                                                                 aCCTGTCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCCACACCATTCAGGAAGTCCGCATTGTTAAAGGGCTGAATAACCCGGATCTCGa                                       <  2:1209037/140‑1 (MQ=255)                                                                                                 aCCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCCACACCATTCAGGAAGTCCGCATTGTTAAAGGGCTGAATAACCCGGAtct                                          >  1:518216/1‑137 (MQ=255)                                                                                                 aCCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCCACACCATTCAGGAAGTCCGCATTGTTAAAGGGCTGAATAACCCGGAtct                                          <  2:518216/137‑1 (MQ=255)                                                                                                 aCCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCCACACCATTCAGGAAGTCCGCATTGTTAAAGGGCTGAATAACCCGGATCTCGa                                       >  1:235299/1‑140 (MQ=255)                                                                                                 aCCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCCACACCATTCAGGAAGTCCGCATTGTTAAAGGGCTGAATAACCCGGATCTCGa                                       <  2:1161521/140‑1 (MQ=255)                                                                                                       ccGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCCACACCATTCAGGAAGTCCGCATTGTTAAAGGGCTGAATAACCCGGATCTCGAtgctgc                                 <  1:960394/140‑1 (MQ=255)                                                                                                         gATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCCACACCATTCAGGAAGTCCGCATTGTTAAAGGGCTGAATAACCCGGATCTCGATGCTGCgg                               <  2:89857/140‑1 (MQ=255)                                                                                                          aTTGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCCACACCATTCAGGAAGTCCGCATTGTTAAAGGGCTGAATAACCCGGATCTCGATGCTGCGGt                              <  1:405670/140‑1 (MQ=255)                                                                                                           ttGGCTGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCCACACCATTCAGGAAGTCCGCATTGTTAAAGGGCTGAATAACCCGGATCTCGATGCTGCGGtt                             >  1:207673/1‑140 (MQ=255)                                                                                                                tGCGGCAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCCACACCATTCAGGAAGTCCGCATTGTTAAAGGGCTGAATAACCCGGATCTCGATGCTGCGGTTGGTGa                        <  2:207673/140‑1 (MQ=255)                                                                                                                     cAAGCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCCACACCATTCAGGAAGTCCGCATTGTTAAAGGGCTGAATAACCCGGATCTCGATGCTGCGGTTGGTGAAGATc                   <  1:1073685/140‑1 (MQ=255)                                                                                                                        gCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCCACACCATTCAGGAAGTCCGCATTGTTAAAGGGCTGAATAACCCGGATCTCGATGCTGCGGTTGGTGAAGATCTgg                <  2:458332/140‑1 (MQ=255)                                                                                                                        gCTGTTTAAAGGCGTTTATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCCACACCATTCAGGAAGTCCGCATTGTTAAAGGGCTGAATAACCCGGATCTCGATGCTGCGGTTGGTGAAGATCTgg                <  1:1279981/140‑1 (MQ=255)                                                                                                                                      tttATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCCACACCATTCAGGAAGTCCGCATTGTTAAAGGGCTGAATAACCCGGATCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGt  >  1:334704/1‑140 (MQ=255)                                                                                                                                      tttATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCCACACCATTCAGGAAGTCCGCATTGTTAAAGGGCTGAATAACCCGGATCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGt  >  1:626532/1‑140 (MQ=255)                                                                                                                                      tttATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCCACACCATTCAGGAAGTCCGCATTGTTAAAGGGCTGAATAACCCGGATCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGt  >  2:155556/1‑140 (MQ=255)                                                                                                                                      tttATCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCCACACCATTCAGGAAGTCCGCATTGTTAAAGGGCTGAATAACCCGGATCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGt  >  1:111254/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        CTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTACCACCTTTATAAAGACGAAACCTATCTCTATCAGAGCGGTAAAGGCCACACCATTCAGGAAGTCCGCATTGTTAAAGGGCTGAATAACCCGGATCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGT  >  NC_000913/4609825‑4610097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑