Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,629,055	A→G	F490L (TTC→CTC)	ettA ←	energy‑dependent translational throttle protein EttA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,629,055	0	A	G	100.0%	253.6 / NA	70	F490L (TTC→CTC)	ettA	energy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/31);  total (39/31)

TTGTACTCTTCGTACTCGGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAGAGT  >  NC_000913/4628922‑4629191                                                                                                                                      |                                                                                                                                         ttGTACTCTTCGTACTCGGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCcag                                                                                                                                    >  1:249807/1‑140 (MQ=255)  tGTACTCTTCGTACTCGGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCcagc                                                                                                                                   <  1:1338688/140‑1 (MQ=255)   gTACTCTTCGTACTCGGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCcagca                                                                                                                                  >  2:869259/1‑140 (MQ=255)         ttCGTACTCGGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACTATCGCACAGCCCGGGAGCTCCAGCAGGGCGt                                                                                                                            >  1:1062652/1‑140 (MQ=255)         ttCGTACTCGGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGt                                                                                                                            >  2:1123581/1‑140 (MQ=255)         ttCGTACTCGGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGt                                                                                                                            >  1:296490/1‑140 (MQ=255)               cTCGGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCa                                                                                                                      <  1:869259/140‑1 (MQ=255)                tCGGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAg                                                                                                                     <  2:290188/140‑1 (MQ=255)                 cGGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAgc                                                                                                                    <  1:231728/140‑1 (MQ=255)                  ggTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAgcg                                                                                                                   >  2:410524/1‑140 (MQ=255)                   gTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAgcgc                                                                                                                  >  2:922963/1‑140 (MQ=255)                   gTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAgcgc                                                                                                                  >  2:371386/1‑140 (MQ=255)                      aaGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAgcgcgcg                                                                                                               <  2:972965/140‑1 (MQ=255)                      aaGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAgcgcgcg                                                                                                               <  2:22654/140‑1 (MQ=255)                       aGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAgcgcgcgc                                                                                                              <  2:296490/140‑1 (MQ=255)                        gTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCa                                                                                                             <  2:1076782/140‑1 (MQ=255)                         ttACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAg                                                                                                            <  2:49604/140‑1 (MQ=255)                          tACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAgg                                                                                                           <  1:371386/140‑1 (MQ=255)                          tACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAgg                                                                                                           <  1:922963/140‑1 (MQ=255)                          tACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAgg                                                                                                           <  1:38233/140‑1 (MQ=255)                          tACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAgg                                                                                                           <  1:82728/140‑1 (MQ=255)                           aCCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAggg                                                                                                          >  2:338605/1‑140 (MQ=255)                            ccTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGt                                                                                                         >  1:729760/1‑140 (MQ=255)                                   agaaCTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCg                                                                                                     <  1:233411/137‑1 (MQ=255)                                   agaaCTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCg                                                                                                     >  2:233411/1‑137 (MQ=255)                                       cTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGGTAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTTCAGCGCGCGCAGGGTTTCGATATcc                                                                                               >  1:177812/1‑139 (MQ=255)                                       cTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGGTAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTTCAGCGCGCGCAGGGTTTCGATATcc                                                                                               <  2:177812/139‑1 (MQ=255)                                                      aTCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTggttggtt                                                                               >  2:1003767/1‑140 (MQ=255)                                                          tGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTtcgtc                                                                           <  2:729760/140‑1 (MQ=255)                                                           ggTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTtcgtcg                                                                          >  2:1171899/1‑140 (MQ=255)                                                           ggTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTtcgtcg                                                                          >  2:1124475/1‑140 (MQ=255)                                                                tCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGagcag                                                                     >  1:169343/1‑140 (MQ=255)                                                                tCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGagcag                                                                     >  1:1270726/1‑140 (MQ=255)                                                                tCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGagcag                                                                     >  1:881576/1‑140 (MQ=255)                                                                tCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGagcag                                                                     >  1:337217/1‑140 (MQ=255)                                                                        gtgCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGt                                                             <  1:1003767/140‑1 (MQ=255)                                                                         tgCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGtt                                                            >  2:1246913/1‑140 (MQ=255)                                                                          gCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCATCAGCATGTTg                                                           >  2:838233/1‑140 (MQ=255)                                                                          gCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTg                                                           >  1:249332/1‑140 (MQ=255)                                                                          gCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTg                                                           >  2:468710/1‑140 (MQ=255)                                                                           cGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGagca                                                                      >  1:530854/1‑128 (MQ=255)                                                                           cGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGagca                                                                      <  2:530854/128‑1 (MQ=255)                                                                           cGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTgc                                                          >  1:84309/1‑140 (MQ=255)                                                                           cGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTgc                                                          >  2:670276/1‑140 (MQ=255)                                                                               gCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTgccgcc                                                      >  1:619957/1‑140 (MQ=255)                                                                                   tACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAAcc                                                  >  1:384150/1‑140 (MQ=255)                                                                                       gTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTgccg                                                        <  2:117534/130‑1 (MQ=255)                                                                                       gTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTgccg                                                        >  1:117534/1‑130 (MQ=255)                                                                                       gTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTgca                                              <  2:60162/140‑1 (MQ=255)                                                                                        tCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTgcag                                             >  2:873736/1‑140 (MQ=255)                                                                                             gAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCACAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCt                                        >  1:1097739/1‑140 (MQ=255)                                                                                                 cAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGCTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGc                                    <  2:189628/140‑1 (MQ=255)                                                                                                  aaCGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGcc                                   >  2:1086546/1‑140 (MQ=255)                                                                                                    cGGTCGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAg                                 <  1:410524/140‑1 (MQ=255)                                                                                                        cGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCt                                        <  1:705204/129‑1 (MQ=255)                                                                                                        cGTGCGAGATAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCt                                        >  2:705204/1‑129 (MQ=255)                                                                                                                aTAACCATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGAcc                     <  1:1086546/140‑1 (MQ=255)                                                                                                                 tAACCATCGCACGGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACcg                    >  1:22536/1‑140 (MQ=255)                                                                                                                     cATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCt                <  1:1246913/140‑1 (MQ=255)                                                                                                                     cATCGCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCt                <  2:84309/140‑1 (MQ=255)                                                                                                                         gctcaGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCTCAGTGTTTCGATATCCAGGTCGGTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTcacc            >  2:864443/4‑140 (MQ=255)                                                                                                                         gCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTcacc            >  2:1344421/1‑140 (MQ=255)                                                                                                                         gCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTcac             <  2:37366/139‑1 (MQ=255)                                                                                                                         gCACAGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTcac             >  1:37366/1‑139 (MQ=255)                                                                                                                          cacaGCCCGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTcacca           <  2:1062652/140‑1 (MQ=255)                                                                                                                               cccGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGga      <  2:619957/140‑1 (MQ=255)                                                                                                                               cccGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGga      <  1:468710/140‑1 (MQ=255)                                                                                                                               cccGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGga      >  1:526716/1‑140 (MQ=255)                                                                                                                               cccGGGAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGga      >  1:980759/1‑140 (MQ=255)                                                                                                                                   ggAGCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAGAGt  <  1:670276/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         TTGTACTCTTCGTACTCGGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAGAGT  >  NC_000913/4628922‑4629191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑