Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	498,410	T→C	V119A (GTA→GCA)	hemH →	ferrochelatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	498,410	0	T	C	100.0%	313.2 / NA	85	V119A (GTA→GCA)	hemH	ferrochelatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/48);  total (37/48)

TCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACGTAGCATTCCGGGGATATCGTTTATACGTGATTACGCCG  >  NC_000913/498274‑498544                                                                                                                                         |                                                                                                                                       tCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGa                                                                                                                                     <  2:586701/140‑1 (MQ=255) tCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGa                                                                                                                                     <  2:948069/140‑1 (MQ=255) tCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGa                                                                                                                                     <  1:625238/140‑1 (MQ=255) tCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGa                                                                                                                                     <  1:1137316/140‑1 (MQ=255)         gATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATtg                                                                                                                             <  1:815970/140‑1 (MQ=255)              tttACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTgtggtg                                                                                                                        <  2:212464/140‑1 (MQ=255)              tttACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTgtggtg                                                                                                                        >  1:1135672/1‑140 (MQ=255)                tACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGtgct                                                                                                                      <  1:1299695/140‑1 (MQ=255)                tACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGtgct                                                                                                                      <  1:471970/140‑1 (MQ=255)                    gccgccAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCg                                                                                                                  >  1:1130210/1‑140 (MQ=255)                    gccgccAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCg                                                                                                                  >  1:657102/1‑140 (MQ=255)                     ccgccAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGc                                                                                                                 >  1:1059261/1‑140 (MQ=255)                      cgccAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCt                                                                                                                <  2:980975/140‑1 (MQ=255)                       gccAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCtt                                                                                                               >  1:217600/1‑140 (MQ=255)                        ccAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCttt                                                                                                              >  1:1128900/1‑140 (MQ=255)                        ccAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCttt                                                                                                              >  2:647712/1‑140 (MQ=255)                           gCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATc                                                                                                           <  2:657102/140‑1 (MQ=255)                             aaCAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCg                                                                                                         <  1:835990/140‑1 (MQ=255)                             aaCAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCg                                                                                                         <  2:1216411/140‑1 (MQ=255)                                agcagGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCaa                                                                                                      <  2:1310871/140‑1 (MQ=255)                                     gcgcTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATtctc                                                                                                 <  2:898347/140‑1 (MQ=255)                                     gcgcTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATtctc                                                                                                 <  1:694281/140‑1 (MQ=255)                                      cgcTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTcc                                                                                                <  1:563499/140‑1 (MQ=255)                                       gcTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCt                                                                                               <  1:970097/140‑1 (MQ=255)                                                 cGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTAcggt                                                                                     <  1:657343/140‑1 (MQ=255)                                                   tttACCGGAGATGCCCGTAGCGCTGGGATTGAGCTACGCCTCCCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTAcggtcg                                                                                   >  1:119764/1‑140 (MQ=255)                                                   tttACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTAcggtcg                                                                                   <  1:696623/140‑1 (MQ=255)                                                     tACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTAcggtcggt                                                                                 <  2:1130210/140‑1 (MQ=255)                                                      aCCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTg                                                                                <  1:647712/140‑1 (MQ=255)                                                      aCCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTg                                                                                <  1:1361697/140‑1 (MQ=255)                                                            gaTGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCg                                                                              >  1:1321642/1‑136 (MQ=255)                                                            gaTGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCg                                                                              <  2:1321642/136‑1 (MQ=255)                                                             aTGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATg                                                                         >  2:885035/1‑140 (MQ=255)                                                               gCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATggg                                                                       >  2:95508/1‑140 (MQ=255)                                                                    tAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGaa                                                                  >  1:928287/1‑140 (MQ=255)                                                                         cTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGt                                                                            >  1:60372/1‑125 (MQ=255)                                                                         cTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGt                                                                            <  2:60372/125‑1 (MQ=255)                                                                         cTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGc                                                             <  2:1128900/140‑1 (MQ=255)                                                                          tGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCa                                                            >  1:695987/1‑140 (MQ=255)                                                                               aTGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCAt                                                       >  2:1104398/1‑140 (MQ=255)                                                                                  aGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCt                                                    >  1:562742/1‑140 (MQ=255)                                                                                   gCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTg                                                   <  2:1059261/140‑1 (MQ=255)                                                                                    cTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTgg                                                  >  2:428194/1‑140 (MQ=255)                                                                                      aCGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTgg                                                  >  2:700063/1‑138 (MQ=255)                                                                                      aCGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTgg                                                  <  1:700063/138‑1 (MQ=255)                                                                                      aCGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGgcg                                                >  1:657841/1‑140 (MQ=255)                                                                                        ggCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGgcgcg                                              <  1:498890/140‑1 (MQ=255)                                                                                        ggCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGgcgcg                                              <  1:95508/140‑1 (MQ=255)                                                                                         gCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGgcgcgc                                             <  2:585072/140‑1 (MQ=255)                                                                                            cGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCaaa                                          >  1:1031820/1‑140 (MQ=255)                                                                                              ccATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACg                                        <  2:1135672/140‑1 (MQ=255)                                                                                                aTCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACGTa                                      <  1:1047493/140‑1 (MQ=255)                                                                                                aTCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACGTa                                      <  1:316238/140‑1 (MQ=255)                                                                                                       gAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACGTAGCATTcc                               >  1:805155/1‑140 (MQ=255)                                                                                                        aaaGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACGTAGCATTCCg                              <  2:119764/140‑1 (MQ=255)                                                                                                         aaGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACGTAGCATTCCgg                             >  2:525757/1‑140 (MQ=255)                                                                                                         aaGCGCCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACGTAGCATTCCgg                             >  2:45816/1‑140 (MQ=255)                                                                                                           gcgcCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACGTAGCATTCCgggg                           >  1:1303644/1‑140 (MQ=255)                                                                                                            cgcCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACGTAGCATTCCgggg                           <  1:1070846/139‑1 (MQ=255)                                                                                                            cgcCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACGTAGCATTCCgggg                           >  2:1070846/1‑139 (MQ=255)                                                                                                            cgcCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACGTAGCATTCCGGGGa                          >  2:1315549/1‑140 (MQ=255)                                                                                                             gcCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACGTAg                                     <  1:769158/128‑1 (MQ=255)                                                                                                             gcCGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACGTAg                                     >  2:769158/1‑128 (MQ=255)                                                                                                              ccGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACGTAGCATTCCGGGGata                        >  2:457400/1‑140 (MQ=255)                                                                                                              ccGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACGTAGCATTCCGGGGata                        <  1:885035/140‑1 (MQ=255)                                                                                                              ccGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACGTAGCATTCCGGGGata                        <  1:525757/140‑1 (MQ=255)                                                                                                              ccGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACGTAGCATTCCGGGGata                        <  1:31854/140‑1 (MQ=255)                                                                                                              ccGTAGATGAACTCCTGGCAGAACATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACGTAGCATTCCGGGGata                        <  2:948061/140‑1 (MQ=255)                                                                                                               cGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACGTAGCATTcc                               >  1:856557/1‑132 (MQ=255)                                                                                                               cGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACGTAGCATTcc                               <  2:856557/132‑1 (MQ=255)                                                                                                               cGTAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACGTAGCATTCCGGGGatat                       >  2:905010/1‑140 (MQ=255)                                                                                                                 tAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACGTAGCATTCCgggg                           <  2:413527/134‑1 (MQ=255)                                                                                                                 tAGATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACGTAGCATTCCgggg                           >  1:413527/1‑134 (MQ=255)                                                                                                                   gATGAACTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACGTAGCATTCCGGGGATATCGtt                   <  1:1315549/140‑1 (MQ=255)                                                                                                                       aaCTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACGTAGCATTCCGGGGATATCGTTtata               <  2:1303644/140‑1 (MQ=255)                                                                                                                       aaCTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACGTAGCATTCCGGGGATATCGTTtata               <  2:1031820/140‑1 (MQ=255)                                                                                                                         cTCCTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACGTAGCATTCCGGGGATATCGTTTATACg             >  1:1281651/1‑140 (MQ=255)                                                                                                                            cTGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACGTAGCATTCCGGGGATATCGTTTATACGTGa          <  2:805155/140‑1 (MQ=255)                                                                                                                             tGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACGTAGCATTCCGGGGata                        <  2:647001/125‑1 (MQ=255)                                                                                                                             tGGCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACGTAGCATTCCGGGGata                        >  1:647001/1‑125 (MQ=255)                                                                                                                              ggCAGAGCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACGTAGCATTCCGGGGATATCGTTTATACGTGAtt        >  1:1331658/1‑140 (MQ=255)                                                                                                                                 agagCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACGTAGCATTCCGGGGATATCGTTTATACGTGATTACg     >  2:1265386/1‑140 (MQ=255)                                                                                                                                   agCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACGTAGCATTCCGGGGATATCGTTTATACGTGATTACGcc   >  1:648254/1‑140 (MQ=255)                                                                                                                                   agCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACGTAGCATTCCGGGGATATCGTTTATACGTGATTACGcc   >  2:494222/1‑140 (MQ=255)                                                                                                                                    gCATGCAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACGTAGCATTCCGGGGATATCGTTTATACGTGATTACGCCg  <  2:657841/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       TCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGGATGAACTGGCACGCATTCTGGCGCGCAAACGTAGCATTCCGGGGATATCGTTTATACGTGATTACGCCG  >  NC_000913/498274‑498544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑