Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	594,037	A→G	V245A (GTA→GCA)	cusS ←	sensory histidine kinase CusS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	594,037	0	A	G	100.0%	214.0 / NA	59	V245A (GTA→GCA)	cusS	sensory histidine kinase CusS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/34);  total (25/34)

GAGGGCGATTTCGGTTTGCGTTATGAGATTCGTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGACAGTACCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGTGACCTTTATGTACCGCCAACAGTACGATAAA  >  NC_000913/593907‑594173                                                                                                                                   |                                                                                                                                         gAGGGCGATTTCGGTTTGCGTTATGAGACTCGTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGACAGTGCCAGCTGtt                                                                                                                                 <  1:1352720/140‑1 (MQ=255)                ttGCGTTATGAGATTCGTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCa                                                                                                                  <  1:1345662/140‑1 (MQ=255)                        tGAGATTCGTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGc                                                                                                          <  1:1130990/140‑1 (MQ=255)                                  aaTTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAAATTGGACTGGCGGGGAAAAAAAACCCCAAAACACCCCGTCATATTGTTGAAAGACAGAGCCAGCCGGTCCCGCCCAATAGGCGCGGGCCGcggggcggggg                                                                                                >  2:502089/1‑139 (MQ=255)                                   aTTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCg                                                                                               <  1:1175980/140‑1 (MQ=255)                                    ttGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGa                                                                                              <  1:502093/140‑1 (MQ=255)                                                  tCGTGAGCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCTAACGTCGAGATCttt                                                                                <  1:764217/140‑1 (MQ=255)                                                  tCGTGAGCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCttt                                                                                <  1:495645/140‑1 (MQ=255)                                                  tCGTGAGCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCttt                                                                                <  1:405954/140‑1 (MQ=255)                                                  tCGTGAGCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCttt                                                                                <  1:610023/140‑1 (MQ=255)                                                  tCGTGAGCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCttt                                                                                <  2:468043/140‑1 (MQ=255)                                                    gTGAGCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCg                                                                              <  2:563535/140‑1 (MQ=255)                                                         cGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTa                                                                         <  1:444137/140‑1 (MQ=255)                                                                 gCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGACAGTGCCAGCTGCTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTg                                                                 <  2:1199556/140‑1 (MQ=255)                                                                       aaaTTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTg                                                           <  1:1229978/140‑1 (MQ=255)                                                                            ggACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCATGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGc                                                      <  2:442087/140‑1 (MQ=255)                                                                               cTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGa                                                   <  2:915502/140‑1 (MQ=255)                                                                                 ggCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACg                                                 <  2:60524/140‑1 (MQ=255)                                                                                            aCATCCTCAATACGCTCGATCATATGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCgg                                      >  2:1367699/1‑140 (MQ=255)                                                                                              aTCCTCAATACGCTCGATCATATGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGgcg                                    >  2:91849/1‑140 (MQ=255)                                                                                                ccTCAATACGCTCGATCATATGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGCGCTCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGcgcg                                  >  2:1216879/1‑140 (MQ=255)                                                                                                ccTCAATACGCTCGATCATATGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGcgcg                                  <  1:619962/140‑1 (MQ=255)                                                                                                ccTCAATACGCTCGATCATATGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGcgcg                                  <  2:819374/140‑1 (MQ=255)                                                                                                ccTCAATACGCTCGATCATATGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGcgcg                                  >  2:1250150/1‑140 (MQ=255)                                                                                                 cTCAATACGCTCGATCATATGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGt                                 >  2:1050608/1‑140 (MQ=255)                                                                                                 cTCAATACGCTCGATCATATGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGt                                 <  1:1216879/140‑1 (MQ=255)                                                                                                   cAATACGCTCGATCATATGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGTGa                               <  1:342893/140‑1 (MQ=255)                                                                                                     aTACGCTCGATCATATGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGTg                                >  2:260871/1‑137 (MQ=255)                                                                                                     aTACGCTCGATCATATGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGTg                                <  1:260871/137‑1 (MQ=255)                                                                                                      tACGCTCGATCATATGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGTGACCt                            >  1:287885/1‑140 (MQ=255)                                                                                                      tACGCTCGATCATATGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGTGACCt                            >  1:1278624/1‑140 (MQ=255)                                                                                                          cTCGATCATATGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGTGACCTTTAt                        >  2:961270/1‑140 (MQ=255)                                                                                                          cTCGATCATATGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGTGACCTTTAt                        <  1:452773/140‑1 (MQ=255)                                                                                                          cTCGATCATATGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGTGACCTTTAt                        >  1:517807/1‑140 (MQ=255)                                                                                                           tCGATCATATGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGGGCGTGACCTTTATg                       >  1:1208768/1‑140 (MQ=255)                                                                                                           tCGATCATATGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGTGACCTTTATg                       >  2:405313/1‑140 (MQ=255)                                                                                                            cGATCATATGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGTGACCTTTATGt                      <  1:502089/140‑1 (MQ=255)                                                                                                              aTCATATGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGTGACCTTTATGTAc                    >  1:1225380/1‑140 (MQ=255)                                                                                                               tCATATGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGTGACCTTTATGTAcc                   <  2:1225380/140‑1 (MQ=255)                                                                                                                    tGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGTGACCTTTATGTACCGCCaa              >  2:606802/1‑140 (MQ=255)                                                                                                                    tGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGTGACCTTTATGTACCGCCaa              <  1:238905/140‑1 (MQ=255)                                                                                                                    tGGTTGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGTGACCTTTATGTACCGCCaa              <  1:858762/140‑1 (MQ=255)                                                                                                                       ttGAACGACAGTGCCAGCTGTTCCAGCTTAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGTGACCTTTATGTACCGCCAACAg           <  1:154019/140‑1 (MQ=255)                                                                                                                       ttGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGTGACCTTTAt                        >  2:937542/1‑127 (MQ=255)                                                                                                                       ttGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGTGACCTTTAt                        <  1:937542/127‑1 (MQ=255)                                                                                                                       ttGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGTGACCTTTATGTACCGCCAACAg           >  1:1206458/1‑140 (MQ=255)                                                                                                                       ttGAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGTGACCTTTATGTACCGCCAACAg           <  2:1278624/140‑1 (MQ=255)                                                                                                                         gAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGTGACCTTTATGTACCGCCaa              <  1:628263/135‑1 (MQ=255)                                                                                                                         gAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGTGACCTTTATGTACCGCCaa              >  2:628263/1‑135 (MQ=255)                                                                                                                         gAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGTGACCTTTATGTACCGCCAACAGTa         >  2:824998/1‑140 (MQ=255)                                                                                                                         gAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGTGACCTTTATGTACCGCCAACAGTa         >  2:1167184/1‑140 (MQ=255)                                                                                                                         gAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGTGACCTTTATGTACCGCCAACAGTa         >  2:179194/1‑140 (MQ=255)                                                                                                                         gAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGTGACCTTTATGTACCGCCAACAGTa         >  2:163692/1‑140 (MQ=255)                                                                                                                         gAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGTGACCTTTATGTACCGCCAACAGTa         >  2:1308849/1‑140 (MQ=255)                                                                                                                         gAACGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGTGACCTTTATGTACCGCCAACAGTa         >  1:569560/1‑140 (MQ=255)                                                                                                                          aaCGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGTGACCTTTATGTACCGCCAACAGt          <  1:140447/138‑1 (MQ=255)                                                                                                                          aaCGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGTGACCTTTATGTACCGCCAACAGt          >  2:140447/1‑138 (MQ=255)                                                                                                                          aaCGACAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGTGACCTTTATGTACCGCCAACAGTAc        <  1:1308849/140‑1 (MQ=255)                                                                                                                              aCAGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGTGACCTTTATGTACCGCCAACAGTACGATa    <  1:1050608/140‑1 (MQ=255)                                                                                                                                aGTGCCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGTGACCTTTATGTACCGCCAACAGTACGATaaa  >  2:628762/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                         GAGGGCGATTTCGGTTTGCGTTATGAGATTCGTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGACAGTACCAGCTGTTCCAGCTCAATGGGCACGGTCTGCGGGTCGAGGCGAACGTCGAGATCTTTCGAGGTAATATTCTGGATTTGACGGCTGACGCTGCGGATCGGCGCGTGACCTTTATGTACCGCCAACAGTACGATAAA  >  NC_000913/593907‑594173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑