BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	78,265	T→C	S215S (AGT→AGC)	setA →	sugar exporter SetA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	78,265	0	T	C	98.3%	427.5 / ‑3.0	119	S215S (AGT→AGC)	setA	sugar exporter SetA
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (55/62); total (56/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 120 total aligned reads displayed.

TACGGTGATGTTTTCGATTGCCGCCGGGATATTCACACTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGTAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGC  >  NC_000913/78130‑78399

  cGGTGATGTTTTCGATTGCCGCCGGGATATTCACACTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTa                                                                                                                                  <  1:1041090/140‑1 (MQ=255)

  cGGTGATGTTTTCGATTGCCGCCGGGATATTCACACTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTa                                                                                                                                  <  2:279745/140‑1 (MQ=255)

     tGATGTTTTCGATTGCCGCCGGGATATTCACACTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACgg                                                                                                                               <  2:443008/140‑1 (MQ=255)

      gATGTTTTCGATTGCCGCCGGGATATTCACACTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGa                                                                                                                              >  1:740298/1‑140 (MQ=255)

       aTGTTTTCGATTGCCGCCGGGATGTTCACACTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGAt                                                                                                                             >  2:936878/1‑140 (MQ=255)

       aTGTTTTCGATTGCCGCCGGGATATTCACACTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTa                                                                                                                                  <  2:77504/135‑1 (MQ=255)

       aTGTTTTCGATTGCCGCCGGGATATTCACACTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTa                                                                                                                                  >  1:77504/1‑135 (MQ=255)

       aTGTTTTCGATTGCCGCCGGGATATTCACACTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGAt                                                                                                                             >  2:904082/1‑140 (MQ=255)

       aTGTTTTCGATTGCCGCCGGGATATTCACACTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGAt                                                                                                                             >  2:255206/1‑140 (MQ=255)

          ttttCGATTGCCGCCGGGATATTCACACTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGtt                                                                                                                          >  1:498813/1‑140 (MQ=255)

             tCGATTGCCGCCGGGATATTCACACTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTAtt                                                                                                                       >  1:663689/1‑140 (MQ=255)

              cGATTGCCGCCGGGATATTCACACTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGtt                                                                                                                          <  2:946625/136‑1 (MQ=255)

              cGATTGCCGCCGGGATATTCACACTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGtt                                                                                                                          >  1:946625/1‑136 (MQ=255)

              cGATTGCCGCCGGGATATTCACACTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTAttt                                                                                                                      >  2:767165/1‑140 (MQ=255)

                 ttGCCGCCGGGATATTCACACTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTc                                                                                                                   >  2:347756/1‑140 (MQ=255)

                   gccgccGGGATATTCACACTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGc                                                                                                                 <  2:586062/140‑1 (MQ=255)

                     cgccgGGATATTCACACTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCt                                                                                                               <  1:171261/140‑1 (MQ=255)

                     cgccgGGATATTCACACTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCt                                                                                                               <  2:740298/140‑1 (MQ=255)

                           gATATTCACACTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGt                                                                                                         <  2:1277628/140‑1 (MQ=255)

                           gATATTCACACTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGt                                                                                                         <  2:239243/140‑1 (MQ=255)

                                  acacTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGC‑GGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTgg                                                                                                 >  1:441753/1‑140 (MQ=255)

                                   cacTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTgg                                                                                                 <  1:1312902/140‑1 (MQ=255)

                                    acTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGa                                                                                                >  1:497078/1‑140 (MQ=255)

                                     cTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGt                                                                                                         >  1:1153954/1‑130 (MQ=255)

                                     cTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGAc                                                                                               >  1:1089253/1‑140 (MQ=255)

                                     cTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGAc                                                                                               <  2:664879/140‑1 (MQ=255)

                                      tCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGAcc                                                                                              >  2:678213/1‑140 (MQ=255)

                                        aGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGTAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTg                                                                                            >  2:878168/1‑140 (MQ=255)

                                        aGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTgg                                                                                                 <  2:338196/135‑1 (MQ=255)

                                        aGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTgg                                                                                                 >  1:338196/1‑135 (MQ=255)

                                         gTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAAtgtg                                                                                                  >  1:783565/1‑133 (MQ=255)

                                         gTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAAtgtg                                                                                                  <  2:783565/133‑1 (MQ=255)

                                         gTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGc                                                                                           >  1:216475/1‑140 (MQ=255)

                                         gTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGc                                                                                           >  1:697603/1‑140 (MQ=255)

                                         gTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGC‑GGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCa                                                                                          <  2:441753/140‑1 (MQ=255)

                                          tCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTgg                                                                                                 >  1:136163/1‑133 (MQ=255)

                                          tCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTgg                                                                                                 <  2:136163/133‑1 (MQ=255)

                                          tCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCa                                                                                          >  1:16758/1‑140 (MQ=255)

                                          tCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCa                                                                                          >  2:1321273/1‑140 (MQ=255)

                                          tCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCa                                                                                          <  1:936878/140‑1 (MQ=255)

                                          tCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCa                                                                                          >  1:1214627/1‑140 (MQ=255)

                                          tCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCa                                                                                          <  1:347756/140‑1 (MQ=255)

                                          tCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCa                                                                                          <  1:255206/140‑1 (MQ=255)

                                          tCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCa                                                                                          >  2:621989/1‑140 (MQ=255)

                                          tCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCa                                                                                          >  1:412818/1‑140 (MQ=255)

                                          tCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCa                                                                                          <  2:75446/140‑1 (MQ=255)

                                             ggTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAaca                                                                                       <  1:621989/140‑1 (MQ=255)

                                             ggTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAaca                                                                                       <  2:46130/140‑1 (MQ=255)

                                             ggTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAaca                                                                                       <  1:956752/140‑1 (MQ=255)

                                              gTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAacac                                                                                      >  1:1012938/1‑140 (MQ=255)

                                              gTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAacac                                                                                      >  1:393588/1‑140 (MQ=255)

                                               tATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGTAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACAcc                                                                                     <  1:878168/140‑1 (MQ=255)

                                               tATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACAcc                                                                                     <  2:1089253/140‑1 (MQ=255)

                                               tATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACAcc                                                                                     <  1:1048013/140‑1 (MQ=255)

                                               tATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACAcc                                                                                     <  2:1324092/140‑1 (MQ=255)

                                                 ttgattgCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCAt                                                                                   <  2:532160/140‑1 (MQ=255)

                                                 ttgattgCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCAt                                                                                   <  2:1214627/140‑1 (MQ=255)

                                                  tgattgCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATg                                                                                  >  2:109859/1‑140 (MQ=255)

                                                  tgattgCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATg                                                                                  <  1:1321273/140‑1 (MQ=255)

                                                  tgattgCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATg                                                                                  >  2:1136942/1‑140 (MQ=255)

                                                    attgCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCaa                                                                                         <  1:352198/131‑1 (MQ=255)

                                                    attgCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCaa                                                                                         >  2:352198/1‑131 (MQ=255)

                                                    attgCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCa                                                                                          >  1:91487/1‑130 (MQ=255)

                                                    attgCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCa                                                                                          <  2:91487/130‑1 (MQ=255)

                                                    attgCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAaca                                                                                       >  2:528796/1‑133 (MQ=255)

                                                    attgCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAaca                                                                                       <  1:528796/133‑1 (MQ=255)

                                                    attgCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTa                                                                                >  2:1366909/1‑140 (MQ=255)

                                                    attgCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTa                                                                                >  1:1162987/1‑140 (MQ=255)

                                                    attgCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTa                                                                                >  2:442131/1‑140 (MQ=255)

                                                        cATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTa                                                                                >  2:1166043/1‑136 (MQ=255)

                                                        cATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTa                                                                                <  1:1166043/136‑1 (MQ=255)

                                                        cATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACatt                                                                            >  2:371918/1‑140 (MQ=255)

                                                              tGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGat                                                                      >  1:31266/1‑140 (MQ=255)

                                                                 ttCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATg                                                                   <  2:216475/140‑1 (MQ=255)

                                                                  tCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGc                                                                  <  1:442131/140‑1 (MQ=255)

                                                                  tCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGc                                                                  <  1:1366909/140‑1 (MQ=255)

                                                                   ccGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGcc                                                                 >  1:651224/1‑140 (MQ=255)

                                                                     gTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGt                                                               <  1:767165/140‑1 (MQ=255)

                                                                     gTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGt                                                               <  1:176376/140‑1 (MQ=255)

                                                                       cTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTtg                                                             <  2:498813/140‑1 (MQ=255)

                                                                         gtgGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTtgtg                                                           <  2:403966/140‑1 (MQ=255)

                                                                           ggCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGa                                                         >  1:497053/1‑140 (MQ=255)

                                                                            gcgcGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGAt                                                        <  2:1012938/140‑1 (MQ=255)

                                                                            gcgcGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGAt                                                        <  2:663689/140‑1 (MQ=255)

                                                                                  gtggAACTGCCGTCGGAAAATGCTTTATGAATGCAAGGTGGCTGGCAGGATAGCAACGTGCGGATGTTATTTGTTGCCTAGGCGTTAATGTGGTTCTACAACACCCTGTGCATTATTGATATGCCGTTGTGGATCAGTAg                                                  <  2:1229652/137‑1 (MQ=255)

                                                                                  gTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAg                                                  <  2:368682/140‑1 (MQ=255)

                                                                                  gTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAg                                                  <  1:107936/140‑1 (MQ=255)

                                                                                    aGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAg                                                  >  1:835815/1‑138 (MQ=255)

                                                                                      aaCTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAg                                              <  1:464868/140‑1 (MQ=255)

                                                                                          gCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAg                                          <  1:507709/140‑1 (MQ=255)

                                                                                            cGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGa                                        >  2:113319/1‑140 (MQ=255)

                                                                                               cGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTg                                     <  2:497078/140‑1 (MQ=255)

                                                                                                ggAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGGCCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGc                                    >  2:1221019/1‑140 (MQ=255)

                                                                                                                aTGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGAtt                                      <  2:170584/122‑1 (MQ=255)

                                                                                                                aTGCAAGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCggg                    >  1:385691/1‑140 (MQ=255)

                                                                                                                    aaGGTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTc                <  1:1221019/140‑1 (MQ=255)

                                                                                                                       gTGGCTGGCAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTg             <  1:334608/140‑1 (MQ=255)

                                                                                                                          gctggcAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATg          >  2:162855/1‑140 (MQ=255)

                                                                                                                               cAGGATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGAc     <  2:497053/140‑1 (MQ=255)

                                                                                                                                  gATAGCAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGGTTCCTGATGGGGACGGc  >  2:1134548/1‑140 (MQ=255)

TACGGTGATGTTTTCGATTGCCGCCGGGATATTCACACTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGTAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGC  >  NC_000913/78130‑78399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`