Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	923,993	A→G	T244A (ACG→GCG)	clpA →	ATP‑dependent Clp protease ATP‑binding subunit ClpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	923,993	0	A	G	100.0%	182.2 / NA	50	T244A (ACG→GCG)	clpA	ATP‑dependent Clp protease ATP‑binding subunit ClpA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/35);  total (15/35)

CTATTCAGGTTCTCTGCCGTCGCCGTAAAAACAACCCGCTGCTGGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCAC  >  NC_000913/923856‑924127                                                                                                                                          |                                                                                                                                       cTATTCAGGTTCTCTGCCGTCGCCGTAAAAACAACCCGCTGCTGGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTGCg                                                                                                                                      >  1:1122421/1‑140 (MQ=255)   aTTCAGGTTCTCTGCCGTCGCCGTAAAAACAACCCGCTGCTGGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTGCGAt                                                                                                                                    <  1:1337612/140‑1 (MQ=255)    ttCAGGTTCTCTGCCGTCGCCGTAAAAACAACCCGCTGCTGGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTGCGAtt                                                                                                                                   >  2:108794/1‑140 (MQ=255)        ggTTCTCTGCCGTCGCCGTAAAAACAACCCGCTGCTGGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTGCGATTTAct                                                                                                                               <  2:427152/140‑1 (MQ=255)            ctctGCCGTCGCCGTAAAAACAACCCGCTGCTGGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTGCGATTTActctct                                                                                                                           <  2:1081249/140‑1 (MQ=255)             tctGCCGTCGCCGTAAAAACAACCCGCTGCTGGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTGCGATTTACtctctc                                                                                                                          <  2:47403/140‑1 (MQ=255)             tctGCCGTCGCCGTAAAAACAACCCGCTGCTGGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTGCGATTTACtctctc                                                                                                                          <  1:492181/140‑1 (MQ=255)                      gCCGTAAAAACAACCCGCTGCTGGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGt                                                                                                                 >  1:1342989/1‑140 (MQ=255)                       ccGTAAAAACAACCCGCTGCTGGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGtt                                                                                                                <  1:1120535/140‑1 (MQ=255)                       ccGTAAAAACAACCCGCTGCTGGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGtt                                                                                                                <  1:927892/140‑1 (MQ=255)                       ccGTAAAAACAACCCGCTGCTGGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGtt                                                                                                                <  1:321304/140‑1 (MQ=255)                                   cccGCTGCTGGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCgg                                                                                                    <  1:460205/140‑1 (MQ=255)                                   cccGCTGCTGGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCgg                                                                                                    <  2:768090/140‑1 (MQ=255)                                          ctgGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACaaa                                                                                             <  1:824542/140‑1 (MQ=255)                                                ggggAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATcg                                                                                       >  2:890635/1‑140 (MQ=255)                                                   gAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCgcgg                                                                                    <  2:1022998/140‑1 (MQ=255)                                                   gAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCgcgg                                                                                    <  1:623151/140‑1 (MQ=255)                                                    aaTCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCgcggc                                                                                   >  2:845794/1‑140 (MQ=255)                                                        tGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACt                                                                               <  2:1122421/140‑1 (MQ=255)                                                             tCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGaa                                                                          <  2:1320263/140‑1 (MQ=255)                                                               ggTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGaaaa                                                                        <  2:308807/140‑1 (MQ=255)                                                                gTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGaaaaa                                                                       >  1:469729/1‑140 (MQ=255)                                                                         cgATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTaaa                                                              >  2:868220/1‑140 (MQ=255)                                                                           aTTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGc                                                            <  2:1093977/140‑1 (MQ=255)                                                                            ttGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCg                                                           <  2:355316/140‑1 (MQ=255)                                                                             tGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGt                                                          <  2:1182280/140‑1 (MQ=255)                                                                                         tGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAagcagc                                              <  2:469729/140‑1 (MQ=255)                                                                                               gCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGc                                        >  2:1355287/1‑140 (MQ=255)                                                                                               gCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGc                                        <  1:108794/140‑1 (MQ=255)                                                                                               gCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGc                                        >  2:1129238/1‑140 (MQ=255)                                                                                               gCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGc                                        <  1:845794/140‑1 (MQ=255)                                                                                                cGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCa                                       >  1:877938/1‑140 (MQ=255)                                                                                                 gAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAg                                      <  1:868220/140‑1 (MQ=255)                                                                                                   aTTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGa                                    <  1:1355287/140‑1 (MQ=255)                                                                                                             gCGATGTGCCGGAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGc                          <  2:1342989/140‑1 (MQ=255)                                                                                                                aTGTGCCGGAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATc                       <  2:802047/140‑1 (MQ=255)                                                                                                                aTGTGCCGGAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATc                       <  1:709989/140‑1 (MQ=255)                                                                                                                 tgtgCCGGAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTaa                              >  2:1309549/1‑132 (MQ=255)                                                                                                                 tgtgCCGGAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTaa                              <  1:1309549/132‑1 (MQ=255)                                                                                                                 tgtgCCGGAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGc                          <  1:1264051/136‑1 (MQ=255)                                                                                                                 tgtgCCGGAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGc                          >  2:1264051/1‑136 (MQ=255)                                                                                                                 tgtgCCGGAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATcc                      <  1:1129238/140‑1 (MQ=255)                                                                                                                       ggAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTa                <  1:332139/140‑1 (MQ=255)                                                                                                                        gAAGTGATGGCTGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTAt               <  2:449319/140‑1 (MQ=255)                                                                                                                              aTGGCTGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATtgatga         >  2:1354765/1‑140 (MQ=255)                                                                                                                                  cTGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATc     <  1:234727/140‑1 (MQ=255)                                                                                                                                   tGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATcc    <  2:877938/140‑1 (MQ=255)                                                                                                                                   tGACTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATcc    >  2:93359/1‑140 (MQ=255)                                                                                                                                     aCTGTGCGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCcac  <  1:93359/140‑1 (MQ=255)                                                                                                                                     aCTGTGCGATTTACTCTCTCGATATCGCTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCcac  >  1:868367/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       CTATTCAGGTTCTCTGCCGTCGCCGTAAAAACAACCCGCTGCTGGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCAC  >  NC_000913/923856‑924127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑