Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,014,202	T→C	V315A (GTA→GCA)	pqiB →	intermembrane transport protein PqiB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,014,202	0	T	C	100.0%	327.4 / NA	88	V315A (GTA→GCA)	pqiB	intermembrane transport protein PqiB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (54/34);  total (54/34)

GTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGAAAATGCGGATGTTGTTGAGCACCTT  >  NC_000913/1014068‑1014338                                                                                                                                       |                                                                                                                                         gTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCa                                                                                                                                     <  2:452378/140‑1 (MQ=255) gTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCa                                                                                                                                     <  1:110883/140‑1 (MQ=255)    tgatgaTCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAg                                                                                                                                  >  1:503444/1‑140 (MQ=255)    tgatgaTCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGATCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAg                                                                                                                                  >  2:73451/1‑140 (MQ=255)      atgatCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTg                                                                                                                                >  2:612690/1‑140 (MQ=255)                 agCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCa                                                                                                                                     <  2:465723/124‑1 (MQ=255)                 agCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCa                                                                                                                                     >  1:465723/1‑124 (MQ=255)                   cATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCTTTCTTTgcgc                                                                                                                   >  2:924046/1‑140 (MQ=255)                   cATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTgcgc                                                                                                                   >  1:1090654/1‑140 (MQ=255)                     ttCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCg                                                                                                                 >  1:880125/1‑140 (MQ=255)                     ttCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCg                                                                                                                 >  2:929452/1‑140 (MQ=255)                              cGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGt                                                                                                        >  2:162256/1‑140 (MQ=255)                                        cGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTa                                                                                              >  1:405039/1‑140 (MQ=255)                                          aTCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAata                                                                                                             <  1:1214999/123‑1 (MQ=255)                                          aTCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAata                                                                                                             >  2:1214999/1‑123 (MQ=255)                                          aTCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAAc                                                                                            >  2:578319/1‑140 (MQ=255)                                          aTCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAAc                                                                                            >  2:54896/1‑140 (MQ=255)                                          aTCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAAc                                                                                            >  2:1032634/1‑140 (MQ=255)                                             acaTTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACgat                                                                                         >  2:209307/1‑140 (MQ=255)                                             acaTTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACgat                                                                                         <  1:209307/140‑1 (MQ=255)                                             acaTTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACgat                                                                                         >  1:1023365/1‑140 (MQ=255)                                               aTTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTa                                                                                              >  1:1315747/1‑133 (MQ=255)                                               aTTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTa                                                                                              <  2:1315747/133‑1 (MQ=255)                                               aTTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACgatga                                                                                       <  1:578319/140‑1 (MQ=255)                                               aTTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACgatga                                                                                       <  1:1032634/140‑1 (MQ=255)                                               aTTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACgatga                                                                                       >  1:61190/1‑140 (MQ=255)                                                 tGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGAtt                                                                                     <  2:678/140‑1 (MQ=255)                                                     tATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCg                                                                                 >  2:1042099/1‑140 (MQ=255)                                                     tATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCg                                                                                 <  1:54896/140‑1 (MQ=255)                                                       tCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAGTATGCGTCAGACATTTAACGATGATTACCGTa                                                                               >  2:967586/1‑140 (MQ=255)                                                       tCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTa                                                                               >  1:290145/1‑140 (MQ=255)                                                       tCTGATGATCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTa                                                                               <  1:73451/140‑1 (MQ=255)                                                         tGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTAtt                                                                             >  2:714869/1‑140 (MQ=255)                                                             gTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCgg                                                                         >  2:535538/1‑140 (MQ=255)                                                               tCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTAtt                                                                             >  1:899146/1‑134 (MQ=255)                                                               tCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTAtt                                                                             <  2:899146/134‑1 (MQ=255)                                                               tCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTa                                                                       <  2:61190/140‑1 (MQ=255)                                                               tCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTa                                                                       <  1:507460/140‑1 (MQ=255)                                                               tCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTa                                                                       <  1:1338571/140‑1 (MQ=255)                                                                cTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTAc                                                                      >  2:1311734/1‑140 (MQ=255)                                                                     aaGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGAtt                                                                 <  1:162256/140‑1 (MQ=255)                                                                        aTTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGTGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGt                                                              >  1:320222/1‑140 (MQ=255)                                                                        aTTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACt                                                                     >  2:683072/1‑133 (MQ=255)                                                                        aTTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACt                                                                     <  1:683072/133‑1 (MQ=255)                                                                        aTTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGt                                                              >  2:1140723/1‑140 (MQ=255)                                                                          tCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTAt                                                            <  1:1302405/140‑1 (MQ=255)                                                                          tCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTAt                                                            <  2:503444/140‑1 (MQ=255)                                                                          tCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTAt                                                            >  1:1137379/1‑140 (MQ=255)                                                                          tCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTAt                                                            >  2:789370/1‑140 (MQ=255)                                                                           cGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGAGATTTAACGATGATTACCGTATTCCGGTACTGATTGGTATc                                                           >  2:1207237/1‑140 (MQ=255)                                                                                  cgGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCag                                                    >  2:61477/1‑140 (MQ=255)                                                                                  cgGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCag                                                    >  2:1337677/1‑140 (MQ=255)                                                                                  cgGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCag                                                    >  2:125972/1‑140 (MQ=255)                                                                                   ggTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCaga                                                   <  2:1113745/140‑1 (MQ=255)                                                                                    gTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCagag                                                  >  2:965164/1‑140 (MQ=255)                                                                                         cAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCt                                             <  1:1207237/140‑1 (MQ=255)                                                                                             cGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCg                                                >  1:1198463/1‑133 (MQ=255)                                                                                             cGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCg                                                <  2:1198463/133‑1 (MQ=255)                                                                                               ggAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAAt                                       >  1:666965/1‑140 (MQ=255)                                                                                               ggAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAAt                                       >  1:1040859/1‑140 (MQ=255)                                                                                               ggAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAAt                                       >  1:1092514/1‑140 (MQ=255)                                                                                               ggAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAAt                                       >  1:1098985/1‑140 (MQ=255)                                                                                               ggAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAAt                                       <  1:789370/140‑1 (MQ=255)                                                                                               ggAGCTCCGGTAGAGTTCCGGGGGATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCGGGAGCGGCTGaaaaa                                       >  2:654448/1‑139 (MQ=255)                                                                                               ggAGCTCCGGTAGAGTTCCAGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAAt                                       >  2:360337/1‑140 (MQ=255)                                                                                                gAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATg                                      <  1:125972/140‑1 (MQ=255)                                                                                                             gTTCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGaaa                         >  2:453809/1‑140 (MQ=255)                                                                                                              ttCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGataa                        >  2:813316/1‑140 (MQ=255)                                                                                                              ttCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGaaaa                        >  2:190292/1‑140 (MQ=255)                                                                                                              ttCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGaaaa                        >  2:1236074/1‑140 (MQ=255)                                                                                                              ttCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGaaaa                        >  2:602820/1‑140 (MQ=255)                                                                                                               tCCGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGAAAAt                       <  2:1098985/140‑1 (MQ=255)                                                                                                                ccGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGaa                          <  1:709628/136‑1 (MQ=255)                                                                                                                ccGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGaa                          >  2:709628/1‑136 (MQ=255)                                                                                                                ccGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGAAAATg                      >  1:1261059/1‑140 (MQ=255)                                                                                                                 cgtggTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGAAAATGc                     <  2:320222/137‑1 (MQ=255)                                                                                                                 cGGGGTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGAAAATGc                     <  1:535538/140‑1 (MQ=255)                                                                                                                   gggTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGAAAATGCgg                   >  2:572472/1‑140 (MQ=255)                                                                                                                   gggTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGAAAATGCgg                   >  1:259893/1‑140 (MQ=255)                                                                                                                    ggTATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGAAAATGCGGa                  <  1:1331548/140‑1 (MQ=255)                                                                                                                      tATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGAAAATGCGGAtg                <  1:1236074/140‑1 (MQ=255)                                                                                                                      tATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGAAAATGCGGAtg                <  2:1261059/140‑1 (MQ=255)                                                                                                                      tATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGAAAATGCGGAtg                <  1:61477/140‑1 (MQ=255)                                                                                                                      tATTCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGAAAATGCGGAtg                <  1:602820/140‑1 (MQ=255)                                                                                                                        ttCGCCTGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGAAAATGCGGAtgtt              >  2:574925/1‑140 (MQ=255)                                                                                                                              tGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGAAAATGCGGATGTTGTTGAg        <  2:1040859/140‑1 (MQ=255)                                                                                                                              tGGGTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGAAAATGCGGATGTTGTTGAg        <  1:867547/140‑1 (MQ=255)                                                                                                                                ggTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGAAAATGCGGATGTTGTTGAGCa      >  2:1167807/1‑140 (MQ=255)                                                                                                                                ggTACCGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGAAAATGCGGATGTTGTTGAGCa      >  1:294213/1‑140 (MQ=255)                                                                                                                                    ccGCAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGAAAATGCGGATGTTGTTGAGCACCtt  >  2:625576/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                         GTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGAAAATGCGGATGTTGTTGAGCACCTT  >  NC_000913/1014068‑1014338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑