Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,029,596 A→G V125A (GTG→GCG)  rlmI ← 23S rRNA m(5)C1962 methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,029,5960AG97.4% 132.1 / ‑4.8 39V125A (GTG→GCG) rlmI23S rRNA m(5)C1962 methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (18/20);  total (19/20)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

CTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCAGGGCACTAATTAATGCCGCGCGCTGATATTCTGCCCCAGCACTCAGCAGTTGCAGCACCAGAAAATTACCGAAACGATCGATAGTAATACCCGGCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGGA  >  NC_000913/1029466‑1029732
                                                                                                                                  |                                                                                                                                        
cTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGATCATTCCGGGTACAGCGTTTGCAGGGCACTAATTAATGCCGCGCGCTGATATTCTGCCCCAGCACTCAGCAGTTGCAGCGCCAGAAAAt                                                                                                                                 >  2:754384/1‑140 (MQ=255)
     ttCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCAGGGCACTAATTAATGCCGCGCGCTGATATTCTGCCCCAGCACTCAGCAGTTGCAGCGCCAGAAAATTACCg                                                                                                                            >  2:363441/1‑140 (MQ=255)
        cttcttTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCAGGGCACTAATTAATGCCGCGCGCTGATATTCTGCCCCAGCACTCAGCAGTTGCAGCGCCAGAAAATTACCGaaa                                                                                                                         <  2:89281/140‑1 (MQ=255)
            ttttttACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCAGGGCACTAATTAATGCCGCGCGCTGATATTCTGCCCCAGCACTCAGCAGTTGCAGCGCCAGAAAATTACCGAAAcgat                                                                                                                     <  1:919886/140‑1 (MQ=255)
             tttttACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCAGGGCACTAATTAATGCCGCGCGCTGATATTCTGCCCCAGCACTCAGCAGTTGCAGCGCCAGAAAATTACCGAAAcgatc                                                                                                                    <  2:1001333/140‑1 (MQ=255)
             tttttACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCAGGGCACTAATTAATGCCGCGCGCTGATATTCTGCCCCAGCACTCAGCAGTTGCAGCGCCAGAAAATTACCGAAAcgatc                                                                                                                    <  2:472825/140‑1 (MQ=255)
             tttttACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCAGGGCACTAATTAATGCCGCGCGCTGATATTCTGCCCCAGCACTCAGCAGTTGCAGCGCCAGAAAATTACCGAAAcgatc                                                                                                                    <  2:1062030/140‑1 (MQ=255)
             tttttACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCAGGGCACTAATTAATGCCGCGCGCTGATATTCTGCCCCAGCACTCAGCAGTTGCAGCGCCAGAAAATTACCGAAAcgatc                                                                                                                    <  2:1033911/140‑1 (MQ=255)
             tttttACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCAGGGCACTAATTAATGCCGCGCGCTGATATTCTGCCCCAGCACTCAGCAGTTGCAGCGCCAGAAAATTACCGAAAcgatc                                                                                                                    <  1:408201/140‑1 (MQ=255)
                    gTACCGCGACGTCGCTGCGATCGTAAATCGATCATTCCGGGTACAGCGTTTGCAGGGCACTAATTAATGCCGCGCGCTGATATTCTGCCCCAGCACTCAGCAGTTGCAGCGCCAGAAAATTACCGAAACGATCGATAGTa                                                                                                             <  1:754384/140‑1 (MQ=255)
                     tACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCAGGTCACTAATTAATGCCGCGCGCTGATATTCTGCCCCAGCACTCAGCAGTTGCAGCGCCAGAAAATTACCGAAAcgatcg                                                                                                                   >  2:263028/1‑133 (MQ=255)
                     tACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCAGGTCACTAATTAATGCCGCGCGCTGATATTCTGCCCCAGCACTCAGCAGTTGCAGCGCCAGAAAATTACCGAAAcgatcg                                                                                                                   <  1:263028/133‑1 (MQ=255)
                       ccGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCAGGGCACTAATTAATGCCGCGCGCTGATATTCTGCCCCAGCACTCAGCAGTTGCAGCGCCAGAAAATTACCGAAACGATCGATAGTAATa                                                                                                          <  2:1142323/140‑1 (MQ=255)
                        cgcgACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCAGGGCACTAATTAATGCCGCGCGCTGATATTCTGCCCCAGCACTCAGCAGTTGCAGCGCCAGAAAATTACCGAAAcgatc                                                                                                                    >  1:1359580/1‑129 (MQ=255)
                        cgcgACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCAGGGCACTAATTAATGCCGCGCGCTGATATTCTGCCCCAGCACTCAGCAGTTGCAGCGCCAGAAAATTACCGAAAcgatc                                                                                                                    <  2:1359580/129‑1 (MQ=255)
                        cgcgACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCAGGGCACTAATTAATGCCGCGCGCTGATATTCTGCCCCAGCACTCAGCAGTTGCAGCGCCAGAAAATTACCGAAACGATCGATAGTAATAc                                                                                                         >  2:1263058/1‑140 (MQ=255)
                          cgACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCAGGGCACTAATTAATGCCGCGCGCTGATATTCTGCCCCAGCACTCAGCAGTTGCAGCGCCAGAAAATTACCGaaa                                                                                                                         <  1:861850/122‑1 (MQ=255)
                          cgACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCAGGGCACTAATTAATGCCGCGCGCTGATATTCTGCCCCAGCACTCAGCAGTTGCAGCGCCAGAAAATTACCGaaa                                                                                                                         >  2:861850/1‑122 (MQ=255)
                          cgACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCAGGGCACTAATTAATGCCGCGCGCTGATATTCTGCCCCAGCACTCAGCAGTTGCAGCACCAGAAAATTACCGAAACGATCGATAGTAATAccc                                                                                                       >  1:1143281/1‑140 (MQ=255)
                                  cTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCAGGGCACTAATTAATGCCGCGCGCTGATATTCTGCCCCAGCACTCAGCAGTTGCAGCGCCAGAAAATTACCGAAACGATCGATAGTAATACCCGGCAGGcc                                                                                               <  1:1123748/140‑1 (MQ=255)
                                                         cGGGTACAGCGTTTGCAGGGCACTAATTAATGCCGCGCGCTGATATTCTGCCCCAGCACTCAGCAGTTGCAGCGCCAGAAAATTACCGAAACGATCGATAGTAATACCCGGCAGGCCATCAGATTCTCCGGCGATTAAAc                                                                        <  2:284794/140‑1 (MQ=255)
                                                             tACAGCGTTTGCAGGGCACTAATTAATGCCGCGCGCTGATATTCTGCCCCAGCACTCAGCAGTTGCAGCGCCAGAAAATTACCGAAACGATCGATAGTAATACCCGGCAGGCCATCAGATTCTCCGGCGATTAAACGATa                                                                    <  2:419818/140‑1 (MQ=255)
                                                               cAGCGTTTGCAGGGCACTAATTAATGCCGCGCGCTGATATTCTGCCCCAGCACTCAGCAGTTGCAGCGCCAGAAAATTACCGAAACGATCGATAGTAATACCCGGCAGGCCATCAGATTCTCCGGCGATTAAAc                                                                        <  1:17440/134‑1 (MQ=255)
                                                               cAGCGTTTGCAGGGCACTAATTAATGCCGCGCGCTGATATTCTGCCCCAGCACTCAGCAGTTGCAGCGCCAGAAAATTACCGAAACGATCGATAGTAATACCCGGCAGGCCATCAGATTCTCCGGCGATTAAAc                                                                        >  2:17440/1‑134 (MQ=255)
                                                                  cGTTTGCAGGGCACTAATTAATGCCGCGCGCTGATATTCTGCCCCAGCACTCAGCAGTTGCAGCGCCAGAAAATTACCGAAACGATCGATAGTAATACCCGGCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGt                                                               >  1:62643/1‑140 (MQ=255)
                                                                     ttGCAGGGCACTAATTAATGCCGCGCGCTGATATTCTGCCCCAGCACTCAGCAGTTGCAGCGCCAGAAAATTACCGAAACGATCGATAGTAATACCCGGCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGa                                                            >  2:1306984/1‑140 (MQ=255)
                                                                           ggCACTAATTAATGCCGCGCGCTGATATTCTGCCCCAGCACTCAGCAGTTGCAGCGCCAGAAAATTACCGAAACGATCGATAGTAATACCCGGCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCAt                                                      <  1:774729/140‑1 (MQ=255)
                                                                                       tGCCGCGCGCTGATATTCTGCCCCAGCACTCAGCAGTTGCAGCGCCAGAAAATTACCGAAACGATCGATAGTAATACCCGGCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCgcca                                          >  1:789023/1‑140 (MQ=255)
                                                                                       tGCCGCGCGCTGATATTCTGCCCCAGCACTCAGCAGTTGCAGCGCCAGAAAATTACCGAAACGATCGATAGTAATACCCGGCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCgcca                                          >  2:799667/1‑140 (MQ=255)
                                                                                        gCCGCGCGCTGATATTCTGCCCCAGCACTCAGCAGTTGCAGCGCCAGAAAATTACCGAAACGATCGATAGTAATACCCGGCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCgccag                                         <  1:656591/140‑1 (MQ=255)
                                                                                                           ccccAGCACTCAGCAGTTGCAGCGCCGGAAAATTACCGAAACGATCGATAGTAATACCCGGCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTtgt                      >  1:89303/1‑140 (MQ=255)
                                                                                                           ccccAGCACTCAGCAGTTGCAGCGCCAGAAAATTACCGAAACGATCGATAGTAATACCCGGCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTtgt                      >  1:391649/1‑140 (MQ=255)
                                                                                                           ccccAGCACTCAGCAGTTGCAGCGCCAGAAAATTACCGAAACGATCGATAGTAATACCCGGCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTtgt                      >  2:842082/1‑140 (MQ=255)
                                                                                                             ccAGCACTCAGCAGTTGCAGCGCCAGAAAATTACCGAAACGATCGATAGTAATACCCGGCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACg                                >  1:801649/1‑128 (MQ=255)
                                                                                                             ccAGCACTCAGCAGTTGCAGCGCCAGAAAATTACCGAAACGATCGATAGTAATACCCGGCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACg                                <  2:801649/128‑1 (MQ=255)
                                                                                                               aGCACTCAGCAGTTGCAGCGCCAGAAAATTACCGAAACGATCGATAGTAATACCCGGCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCtt                  <  1:1306984/140‑1 (MQ=255)
                                                                                                                     cagcagTTGCAGCGCCAGAAAATTACCGAAACGATCGATAGTAATACCCGGCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCa            >  2:381014/1‑140 (MQ=255)
                                                                                                                           ttGCAGCGCCAGAAAATTACCGAAACGATCGATAGTAATACCCGGCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAAcggc      >  1:558296/1‑140 (MQ=255)
                                                                                                                           ttGCAGCGCCAGAAAATTACCGAAACGATCGATAGTAATACCCGGCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAAcggc      >  1:1240289/1‑140 (MQ=255)
                                                                                                                             gcagcgCCAGAAAATTACCGAAACGATCGATAGTAATACCCGGCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAAcggcgg    >  1:87976/1‑140 (MQ=255)
                                                                                                                               agcaCCAGAAAATTACCGAAACGATCGATAGTAATACCCGGCAGGCCATCAGATTCTCCGGCGATTAACCGATAGCTGTCGAGGCCATCTTTTGGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGGa  <  2:1143281/140‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                        
CTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCAGGGCACTAATTAATGCCGCGCGCTGATATTCTGCCCCAGCACTCAGCAGTTGCAGCACCAGAAAATTACCGAAACGATCGATAGTAATACCCGGCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGGA  >  NC_000913/1029466‑1029732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑