Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	128,349	T→C	G146G (GGT→GGC)	lpd →	lipoamide dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	128,349	0	T	C	97.8%	309.6 / ‑2.5	89	G146G (GGT→GGC)	lpd	lipoamide dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (39/48);  total (40/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01

TGGCTGGTATGGCGAAAGGCCGCAAAGTCAAAGTGGTCAACGGTCTGGGTAAATTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGTTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGAAAGAAGTACCAGAACGCCTGCTGGTAATGGGTGGCGGTATCATCGGTCTGGAAATGGGC  >  NC_000913/128213‑128484                                                                                                                                         |                                                                                                                                        tggctggTATGGCGAAAGGCCGCAAAGTCAAAGTGGTCAACGGTCTGGGTAAATTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGctct                                                                                                                                      >  1:1030587/1‑140 (MQ=255) tggctggTATGGCGAAAGGCCGCAAAGTCAAAGTGGTCAACGGTCTGGGTAAATTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGctct                                                                                                                                      >  1:677346/1‑140 (MQ=255)  ggctggTATGGCGAAAGGCCGCAAAGTCAAAGTGGTCAACGGTCTGGGTAAATTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCtctc                                                                                                                                     >  1:365586/1‑140 (MQ=255)      ggTATGGCGAAAGGCCGCAAAGTCAAAGTGGTCAACGGTCTGGGTAAATTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGccc                                                                                                                                 >  2:1113036/1‑140 (MQ=255)      ggTATGGCGAAAGGCCGCAAAGTCAAAGTGGTCAACGGTCTGGGTAAATTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGccc                                                                                                                                 >  2:853439/1‑140 (MQ=255)        tATGGCGAAAGGCCGCAAAGTCAAAGTGGTCAACGGTCTGGGTAAATTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGa                                                                                                                               >  1:64563/1‑140 (MQ=255)        tATGGCGAAAGGCCGCAAAGTCAAAGTGGTCAACGGTCTGGGTAAATTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGa                                                                                                                               >  1:15496/1‑140 (MQ=255)             cGAAAGGCCGCAAAGTCAAAGTGGTCAACGGTCTGGGTAAATTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCaa                                                                                                                          >  2:103801/1‑140 (MQ=255)             cGAAAGGCCGCAAAGTCAAAGTGGTCAACGGTCTGGGTAAATTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCaa                                                                                                                          >  2:1248394/1‑140 (MQ=255)             cGAAAGGCCGCAAAGTCAAAGTGGTCAACGGTCTGGGTAAATTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCaa                                                                                                                          >  1:145805/1‑140 (MQ=255)               aaaGGCCGCAAAGTCAAAGTGGTCAACGGTCTGGGTAAATTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACt                                                                                                                        <  2:680329/140‑1 (MQ=255)                 aGGCCGCAAAGTCAAAGTGGTCAACGGTCTGGGTAAATTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGc                                                                                                                      >  2:972619/1‑140 (MQ=255)                  ggCCGCAAAGTCAAAGTGGTCAACGGTCTGGGTAAATTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGcc                                                                                                                     >  2:855486/1‑140 (MQ=255)                      gCAAAGTCAAAGTGGTCAACGGTCTGGGTAAATTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGttt                                                                                                                 >  1:1242761/1‑140 (MQ=255)                            tcaaagtGGTCAACGGTCTGGGTAAATTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTa                                                                                                                >  2:244519/1‑135 (MQ=255)                            tcaaagtGGTCAACGGTCTGGGTAAATTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTa                                                                                                                <  1:244519/135‑1 (MQ=255)                               aagtGGTCAACGGTCTGGGTAAATTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCAt                                                                                                        <  2:64563/140‑1 (MQ=255)                                    gTCAACGGTCTGGGTAAGTTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTAtt                                                                                                              <  1:561010/129‑1 (MQ=255)                                    gTCAACGGTCTGGGTAAGTTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTAtt                                                                                                              >  2:561010/1‑129 (MQ=255)                                      cAACGGTCTGGGTAAATTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATc                                                                                                 >  1:516897/1‑140 (MQ=255)                                        aCGGTCTGGGTAAATTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCcg                                                                                               <  1:853439/140‑1 (MQ=255)                                         cGGTCTGGGTAAATTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCcgc                                                                                              <  2:677346/140‑1 (MQ=255)                                         cGGTCTGGGTAAATTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCcgc                                                                                              <  2:628846/140‑1 (MQ=255)                                         cGGTCTGGGTAAATTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCcgc                                                                                              <  1:1248394/140‑1 (MQ=255)                                            tCTGGGTAAATTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCcgc                                                                                              <  2:97316/137‑1 (MQ=255)                                            tCTGGGTAAATTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCcgc                                                                                              >  1:97316/1‑137 (MQ=255)                                            tCTGGGTAAATTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTa                                                                                           >  1:1325215/1‑140 (MQ=255)                                            tCTGGGTAAATTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTa                                                                                           >  2:714252/1‑140 (MQ=255)                                              tGGGTAAATTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATc                                                                                         >  2:520164/1‑140 (MQ=255)                                                 gTAAATTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTgg                                                                                      <  2:516897/140‑1 (MQ=255)                                                 gTAAATTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTgg                                                                                      <  1:1325627/140‑1 (MQ=255)                                                   aaaTTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAATGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGa                                                                                    <  2:992144/140‑1 (MQ=255)                                                     aTTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGa                                                                                    <  1:1068356/138‑1 (MQ=255)                                                     aTTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGa                                                                                    >  2:1068356/1‑138 (MQ=255)                                                     aTTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACt                                                                                  >  2:766829/1‑140 (MQ=255)                                                      ttCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTc                                                                                 >  1:378010/1‑140 (MQ=255)                                                           cGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTg                                                                            >  1:24109/1‑140 (MQ=255)                                                            ggggCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGa                                                                           >  2:997832/1‑140 (MQ=255)                                                                cTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGAcgcg                                                                       <  1:997832/140‑1 (MQ=255)                                                                cTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGAcgcg                                                                       >  2:1104530/1‑140 (MQ=255)                                                                     acCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGa                                                                  <  2:24109/140‑1 (MQ=255)                                                                      cccTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGaa                                                                 <  1:45914/140‑1 (MQ=255)                                                                      cccTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGaa                                                                 <  1:714252/140‑1 (MQ=255)                                                                        cTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACt                                                               <  2:15496/140‑1 (MQ=255)                                                                        cTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACt                                                               <  2:107306/140‑1 (MQ=255)                                                                         tGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTg                                                              >  1:952068/1‑140 (MQ=255)                                                                         tGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTg                                                              >  1:1010897/1‑140 (MQ=255)                                                                         tGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGAACCGCGTATCTGGGACTCCACTGACGCGCTGGAACTg                                                              <  1:855486/140‑1 (MQ=255)                                                                          ggAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGa                                                             >  1:1262524/1‑140 (MQ=255)                                                                           gAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGaa                                                            <  2:145805/140‑1 (MQ=255)                                                                             aGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGAaag                                                          <  2:378010/140‑1 (MQ=255)                                                                             aGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAAAGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGAaag                                                          <  2:1262524/140‑1 (MQ=255)                                                                               ttGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGaa                                                            >  1:346759/1‑136 (MQ=255)                                                                               ttGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGaa                                                            <  2:52214/136‑1 (MQ=255)                                                                               ttGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGaa                                                            >  1:52214/1‑136 (MQ=255)                                                                               ttGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGaa                                                            <  2:346759/136‑1 (MQ=255)                                                                                tGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGAaagaag                                                       <  1:103801/140‑1 (MQ=255)                                                                                 gAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTTACGCGCTGGAACTGAAAGAAGt                                                      >  2:1050333/1‑140 (MQ=255)                                                                                 gAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGaa                                                            <  1:659299/134‑1 (MQ=255)                                                                                 gAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGaa                                                            >  2:659299/1‑134 (MQ=255)                                                                                 gAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGAAAGAAGt                                                      >  1:372673/1‑140 (MQ=255)                                                                                 gAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGAAAGAAGt                                                      >  1:454466/1‑140 (MQ=255)                                                                                     gTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGAAAGAAGTACCa                                                  <  2:312867/140‑1 (MQ=255)                                                                                     gTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATAAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGAAAGAAGTACCa                                                  >  1:680332/1‑140 (MQ=255)                                                                                           aCGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGAAAGAAGTACCAGAACGc                                            <  1:1113036/140‑1 (MQ=255)                                                                                            cGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATAAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGAAAGAAGTACCAGAACGcc                                           <  2:680332/140‑1 (MQ=255)                                                                                              gCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAGCTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGAAAGAAGTACCAGAACGCctg                                         <  1:972619/140‑1 (MQ=255)                                                                                               cAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGAAAGAAGTACCAGAACGCctgc                                        >  2:1061985/1‑140 (MQ=255)                                                                                               cAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGAAAGAAGTACCAGAACGCctgc                                        <  2:1242761/140‑1 (MQ=255)                                                                                                 aaaCCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTTACGCGCTGGAACTGAAAGAAGTACCAGAACGCctgctg                                      <  1:1050333/140‑1 (MQ=255)                                                                                                        gATCAACTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGAAAGAAGTACCAGAACGCCTGCTGGTAATgg                               <  1:767191/140‑1 (MQ=255)                                                                                                            aaCTTCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATACCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGAAAGAAGTACCAGAACGCCTGCTGGTAATGGGTgg                           <  1:902690/140‑1 (MQ=255)                                                                                                               ttCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGAAAGAAGTACCAGAACGCCTGCTGGTAATGGGTGGCgg                        <  1:1061985/140‑1 (MQ=255)                                                                                                               ttCGACAACGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGAAAGAAGTACCAGAACGCCTGCTGGTAATGGGTGGCgg                        <  2:952068/140‑1 (MQ=255)                                                                                                                    cAACGCGATCATTGCAGCGGGTTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGAAAGAAGTACCAGAACGCCTGCTGGTAATGGGTGGCGGTatca                   <  2:664110/140‑1 (MQ=255)                                                                                                                    cAACGCGATCATTGCAGCGGGTTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGAAAGAAGTACCAGAACGCCTGCTGGTAATGGGTGGCGGTatca                   >  1:664110/1‑140 (MQ=255)                                                                                                                     aaCGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGAAAGAAGTACCAGAACGCCTGCTGGTAATGGGTGGCGGTatcat                  <  2:1333931/140‑1 (MQ=255)                                                                                                                     aaCGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGAAAGAAGTACCAGAACGCCTGCTGGTAATGGGTGGCGGTatcat                  <  2:458164/140‑1 (MQ=255)                                                                                                                     aaCGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGAAAGAAGTACCAGAACGCCTGCTGGTAATGGGTGGCGGTatcat                  <  2:365586/140‑1 (MQ=255)                                                                                                                     aaCGCGATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGAAAGAAGTACCAGAACGCCTGCTGGTAATGGGTGGCGGTatcat                  <  1:1338440/140‑1 (MQ=255)                                                                                                                        gcgATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGAAAGAAGTACCAGAACGCCTGCTGGTAATGGGTGGCGGTATCATCgg               >  2:1110407/1‑140 (MQ=255)                                                                                                                         cgATCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGAAAGAAGTACCAGAACGCCTGCTGGTAATGGGTGGCGGTATCATCGGt              >  2:1301690/1‑140 (MQ=255)                                                                                                                           aTCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGAAAGAAGTACCAGAACGCCTGCTGGTAATGGGTGGCGGTATCATCGGTCt            >  1:519743/1‑140 (MQ=255)                                                                                                                            tCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGAAAGAAGTACCAGAACGCCTGCTGGTAATGGGTGGCGGTATCATCGGTCTg           <  2:131008/140‑1 (MQ=255)                                                                                                                            tCATTGCAGCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGAACTCCACTGACGCGCTGGAACTGAAAGAAGTACCAGAACGCCTGCTGGTAATGGGTGGCGGTATCATCGGTCTg           <  1:1229110/140‑1 (MQ=255)                                                                                                                                    gCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGAAAGAAGTACCAGAACGCCTGCTGGTAATGGGTGGCGGTATCATCGGTCTGGAAATggg   <  1:1104530/140‑1 (MQ=255)                                                                                                                                    gCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGAAAGAAGTACCAGAACGCCTGCTGGTAATGGGTGGCGGTATCATCGGTCTGGAAATggg   <  2:519743/140‑1 (MQ=255)                                                                                                                                    gCGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGAAAGAAGTACCAGAACGCCTGCTGGTAATGGGTGGCGGTATCATCGGTCTGAAAATggg   <  2:694216/140‑1 (MQ=255)                                                                                                                                     cGGGCTCTCGCCCGATCCAGCTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGAAAGAAGGACCAGAACGCCTGCTGGTAATGCGTGGCGGCATCATCGGTCTGGAAATGAGc  >  2:1085081/1‑140 (MQ=255)                                                                                                                                     cGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGAAAGAAGTACCAGAACGCCTGCTGGTAATGGGTGGCGGTATCATCGGTCTGGAAATGGGc  <  2:72340/140‑1 (MQ=255)                                                                                                                                     cGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGAAAGAAGTACCAGAACGCCTGCTGGTAATGGGTGGCGGTATCATCGGTCTGGAAATGGGc  <  2:1010897/140‑1 (MQ=255)                                                                                                                                     cGGGCTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGAAAGAAGTACCAGAACGCCTGCTGGTAATGGGTGGCGGTATCATCGGTCCGGAAATGGGc  >  2:15986/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        TGGCTGGTATGGCGAAAGGCCGCAAAGTCAAAGTGGTCAACGGTCTGGGTAAATTCACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGTTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCACTGACGCGCTGGAACTGAAAGAAGTACCAGAACGCCTGCTGGTAATGGGTGGCGGTATCATCGGTCTGGAAATGGGC  >  NC_000913/128213‑128484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑