Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,280,410	C→T	P183S (CCG→TCG)	narG →	nitrate reductase A subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,280,410	0	C	T	100.0%	188.8 / NA	54	P183S (CCG→TCG)	narG	nitrate reductase A subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/29);  total (25/29)

CTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGTGGACGCGGTGGATTTGTTCGTTCTTCCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGT  >  NC_000913/1280276‑1280545                                                                                                                                       |                                                                                                                                        cTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGTGGACGCGGTGGATTTGTTCGTTCTTCCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGAc                                                                                                                                    <  1:882664/140‑1 (MQ=255)            gACGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGTGGACGCGGTGGATTTGTTCGTTCTTCCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCt                                                                                                                           <  1:782775/138‑1 (MQ=255)            gACGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGTGGACGCGGTGGATTTGTTCGTTCTTCCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCt                                                                                                                           >  2:782775/1‑138 (MQ=255)            gACGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGTGGACGCGGTGGATTTGTTCGTTCTTCCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTgg                                                                                                                         >  1:781736/1‑140 (MQ=255)             aCGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGTGGACGCGGTGGATTTGTTCGTTCTTCCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGt                                                                                                                        >  2:1184220/1‑140 (MQ=255)             aCGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGTGGACGCGGTGGATTTGTTCGTTCTTCCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGt                                                                                                                        >  2:20995/1‑140 (MQ=255)              cGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGTGGACGCGGTGGATTTGTTCGTTCTTCCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGtt                                                                                                                       >  2:1157856/1‑140 (MQ=255)              cGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGTGGACGCGGTGGATTTGTTCGTTCTTCCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGtt                                                                                                                       >  1:338420/1‑140 (MQ=255)              cGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGTGGACGCGGTGGATTTGTTCGTTCTTCCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGtt                                                                                                                       >  1:918345/1‑140 (MQ=255)                ccGATAAAGCGAAAAGCTTTAAGCAGGCGCGTGGACGCGGTGGATTTGTTCGTTCTTCCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTtc                                                                                                                     <  2:918345/140‑1 (MQ=255)                    tAAAGCGAAAAGCTTTAAGCAGGCGCGTGGACGCGGTGGATTTGTTCGTTCTTCCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGc                                                                                                                 >  2:320934/1‑140 (MQ=255)                         cGAAAAGCTTTAAGCAGGCGCGTGGACGCGGTGGATTTGTTCGTTCTTCCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAAtt                                                                                                            <  2:597105/140‑1 (MQ=255)                           aaaaGCTTTAAGCAGGCGCGTGGACGCGGTGGATTTGTTCGTTCTTCCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTcc                                                                                                          >  1:950132/1‑140 (MQ=255)                               gCTTTAAGCAGGCGCGTGGACGCGGTGGATTTGTTCGTTCTTCCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCa                                                                                                      <  2:338420/140‑1 (MQ=255)                                     aGCAGGCGCGTGGACGCGGTGGATTTGTTCGTTCTTCCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCg                                                                                                <  2:781736/140‑1 (MQ=255)                                        aGGCGCGTGGACGCGGTGGATTTGTTCGTTCTTCCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATg                                                                                             <  1:450516/140‑1 (MQ=255)                                        aGGCGCGTGGACGCGGTGGATTTGTTCGTTCTTCCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATg                                                                                             <  2:1045314/140‑1 (MQ=255)                                          gcgcGTGGACGCGGTGGATTTGTTCGTTCTTCCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGt                                                                                           >  2:157793/1‑140 (MQ=255)                                              gTGGACGCGGTGGATTTGTTCGTTCTTCCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCt                                                                                       <  1:1352093/140‑1 (MQ=255)                                               tGGACGCGGTGGATTTGTTCGTTCTTCCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCtt                                                                                      >  2:38192/1‑140 (MQ=255)                                                ggACGCGGTGGATTTGTTCGTTCTTCCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTa                                                                                     <  1:1251494/140‑1 (MQ=255)                                                          gATTTGTTCGTTCTTCCTGGCAGGAGGTGAACGAACTGATCGCTGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGt                                                                           <  2:436075/140‑1 (MQ=255)                                                           aTTTGTTCGTTCTTCCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTg                                                                          <  1:1184220/140‑1 (MQ=255)                                                           aTTTGTTCGTTCTTCCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTg                                                                          <  1:20995/140‑1 (MQ=255)                                                                ttcgttcTTCCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGGTTCTTACGCATCGGGGGCACGc                                                                     >  2:876773/1‑140 (MQ=255)                                                                  cgttcTTCCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTa                                                                   >  2:892010/1‑140 (MQ=255)                                                                    ttcttcCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTg                                                                          <  1:633831/131‑1 (MQ=255)                                                                    ttcttcCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTg                                                                          >  2:633831/1‑131 (MQ=255)                                                                       ttcCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTAtctct                                                              <  1:157793/140‑1 (MQ=255)                                                                         ccTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCg                                                            <  2:809474/140‑1 (MQ=255)                                                                              cAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGAt                                                       <  1:38192/140‑1 (MQ=255)                                                                                 gaggTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTgg                                                    >  1:986739/1‑140 (MQ=255)                                                                                 gaggTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTgg                                                    <  1:320934/140‑1 (MQ=255)                                                                                 gaggTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTgg                                                    >  1:34371/1‑140 (MQ=255)                                                                                  aggTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGc                                                   >  2:1278534/1‑140 (MQ=255)                                                                                      gaacgaacTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTa                                               <  1:504216/140‑1 (MQ=255)                                                                                       aacgaacTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGCCCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGATTCTTACGCATCGGGTGCACGCTATCTCTCGCTGCTTTGCGGGAc                                              >  2:399218/1‑140 (MQ=255)                                                                                       aacgaacTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGcc                                                                                                                >  1:766653/1‑74 (MQ=255)                                                                                       aacgaacTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGcc                                                                                                                <  2:766653/74‑1 (MQ=255)                                                                                            acTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCt                                         <  1:1278534/140‑1 (MQ=255)                                                                                            acTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCt                                         <  1:608366/140‑1 (MQ=255)                                                                                            acTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCt                                         >  1:478948/1‑140 (MQ=255)                                                                                             cTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                        <  2:478948/140‑1 (MQ=255)                                                                                              tGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTa                                       <  2:34371/140‑1 (MQ=255)                                                                                                     cgcATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCt                                <  2:986739/140‑1 (MQ=255)                                                                                                       cATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTTAGCTTCttc                              >  2:37332/1‑138 (MQ=255)                                                                                                       cATCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTAc                              >  2:146994/1‑140 (MQ=255)                                                                                                        aTCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCt                                >  1:98124/1‑137 (MQ=255)                                                                                                        aTCTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCt                                <  2:98124/137‑1 (MQ=255)                                                                                                          cTAACGTTTACACCATCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGAc                           <  1:399218/140‑1 (MQ=255)                                                                                                                         tCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTg            <  1:1299770/140‑1 (MQ=255)                                                                                                                         tCAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTg            <  1:654875/140‑1 (MQ=255)                                                                                                                          cAAAAACTACGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGc           >  1:551921/1‑140 (MQ=255)                                                                                                                                   cGGCTCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGt  >  1:357651/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        CTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGTGGACGCGGTGGATTTGTTCGTTCTTCCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGT  >  NC_000913/1280276‑1280545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑