Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	142,194	G→A	G159G (GGC→GGT)	can ←	carbonic anhydrase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	142,194	0	G	A	100.0%	338.0 / NA	96	G159G (GGC→GGT)	can	carbonic anhydrase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (47/49);  total (47/49)

TAACGTTGCTCAAGGGTTTCGCGGTTGGTGGCGGTAACATCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGGCCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAGCAATGAGCTATGTTTGAACCAGATATCGCGGATATGCAGCAGCCAGTTGTTGATAAGC  >  NC_000913/142057‑142329                                                                                                                                          |                                                                                                                                        tAACGTTGCTCAAGGGTTTCGCGGTTGGTGGCGGTAACATCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGAcc                                                                                                                                       <  1:534451/140‑1 (MQ=255)  aaCGTTGCTCAAGGGTTTCGCGGTTGGTGGCGGTAACATCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGAccc                                                                                                                                      <  1:1318548/140‑1 (MQ=255)   aCGTTGCTCAAGGGTTTCGCGGTTGGTGGCGGTAACATCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCa                                                                                                                                     <  1:902343/140‑1 (MQ=255)   aCGTTGCTCAAGGGTTTCGCGGTTGGTGGCGGTAACATCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCa                                                                                                                                     <  1:186757/140‑1 (MQ=255)   aCGTTGCTCAAGGGTTTCGCGGTTGGTGGCGGTAACATCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCATTTCCACGCTGATTGCATAATGGTGGAGTGACCCa                                                                                                                                     <  2:26355/140‑1 (MQ=255)    cGTTGCTCAAGGGTTTCGCGGTTGGTGGCGGTAACATCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAg                                                                                                                                    >  1:1170059/1‑140 (MQ=255)       tGCTCAAGGGTTTCGCGGTTGGTGGCGGTAACATCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGtt                                                                                                                                 >  1:727969/1‑140 (MQ=255)        gCTCAAGGGTTTCGCGGTTGGTGGCGGTAACATCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTta                                                                                                                                <  1:57491/140‑1 (MQ=255)        gCTCAAGGGTTTCGCGGTTGGTGGCGGTAACATCCAGATCACGCAGCAAGCCGTCGTGAACGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTta                                                                                                                                >  2:979550/1‑140 (MQ=255)         cTCAAGGGTTTCGCGGTTGGTGGCGGTAACATCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGAccc                                                                                                                                      >  1:1120858/1‑133 (MQ=255)         cTCAAGGGTTTCGCGGTTGGTGGCGGTAACATCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGAccc                                                                                                                                      <  2:1120858/133‑1 (MQ=255)         cTCAAGGGTTTCGCGGTTGGTGGCGGTAACATCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTtat                                                                                                                               >  1:1344729/1‑140 (MQ=255)         cTCAAGGGTTTCGCGGTTGGTGGCGGTAACATCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTtat                                                                                                                               >  2:551357/1‑140 (MQ=255)         cTCAAGGGTTTCGCGGTTGGTGGCGGTAACATCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTtat                                                                                                                               <  2:727969/140‑1 (MQ=255)             aGGGTTTCGCGGTTGGTGGCGGTAACATCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATacac                                                                                                                           <  1:32494/140‑1 (MQ=255)             aGGGTTTCGCGGTTGGTGGCGGTAACATCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATacac                                                                                                                           <  2:1344729/140‑1 (MQ=255)             aGGGTTTCGCGGTTGGTGGCGGTAACATCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATacac                                                                                                                           <  1:800171/140‑1 (MQ=255)              gggTTTCGCGGTTGGTGGCGGTAACATCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACAcc                                                                                                                          >  1:1116250/1‑140 (MQ=255)                 tttCGCGGTTGGTGGCGGTAACATCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATacac                                                                                                                           >  2:763062/1‑136 (MQ=255)                 tttCGCGGTTGGTGGCGGTAACATCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATacac                                                                                                                           <  1:763062/136‑1 (MQ=255)                   tCGCGGTTGGTGGCGGTAACATCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTc                                                                                                                     >  1:142098/1‑140 (MQ=255)                   tCGCGGTTGGTGGCGGTAACATCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTc                                                                                                                     >  2:872359/1‑140 (MQ=255)                   tCGCGGTTGGTGGCGGTAACATCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTc                                                                                                                     >  2:764171/1‑140 (MQ=255)                   tCGCGGTTGGTGGCGGTAACATCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTc                                                                                                                     >  1:1208642/1‑140 (MQ=255)                      cgGTTGGTGGCGGTAACATCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCAt                                                                                                                  >  2:466610/1‑140 (MQ=255)                         ttGGTGGCGGTAACATCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGAc                                                                                                               >  1:70129/1‑140 (MQ=255)                           ggtggCGGTAACATCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACgt                                                                                                             >  1:432961/1‑140 (MQ=255)                            gtggCGGTAACATCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACgtt                                                                                                            <  2:779700/140‑1 (MQ=255)                                cGGTAACATCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACgttcagt                                                                                                        <  1:551357/140‑1 (MQ=255)                                   tAACATCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTcc                                                                                                                    >  2:1020409/1‑125 (MQ=255)                                   tAACATCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTcc                                                                                                                    <  1:1020409/125‑1 (MQ=255)                                    aaCATCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTcac                                                                                                    <  2:1154471/140‑1 (MQ=255)                                    aaCATCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTcac                                                                                                    <  1:872359/140‑1 (MQ=255)                                    aaCATCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTcac                                                                                                    <  2:432961/140‑1 (MQ=255)                                     aCATCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTcaca                                                                                                   >  1:719463/1‑140 (MQ=255)                                       aTCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTcacaca                                                                                                 <  1:466610/140‑1 (MQ=255)                                       aTCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTcacaca                                                                                                 <  1:764171/140‑1 (MQ=255)                                        tCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACaa                                                                                                >  2:380692/1‑140 (MQ=255)                                        tCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACaa                                                                                                <  1:379781/140‑1 (MQ=255)                                           aGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGt                                                                                             <  1:755123/140‑1 (MQ=255)                                             aTCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTAt                                                                                           >  1:879576/1‑140 (MQ=255)                                               cACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTTTACACCTGTTCCATGACGTTCAGTTCACACAAGGTATcc                                                                                         <  1:90163/140‑1 (MQ=255)                                               cACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATcc                                                                                         >  1:1235795/1‑140 (MQ=255)                                                aCGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCa                                                                                        <  1:732564/140‑1 (MQ=255)                                                 cGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAg                                                                                       >  2:258916/1‑140 (MQ=255)                                                 cGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAg                                                                                       >  2:346536/1‑140 (MQ=255)                                                 cGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAg                                                                                       >  2:876196/1‑140 (MQ=255)                                                 cGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAg                                                                                       >  2:995240/1‑140 (MQ=255)                                                 cGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAg                                                                                       >  1:488507/1‑140 (MQ=255)                                                  gcagcaAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGa                                                                                      <  1:995240/140‑1 (MQ=255)                                                       aaGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGa                                                                                      >  1:1359698/1‑135 (MQ=255)                                                       aaGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGa                                                                                      <  2:1359698/135‑1 (MQ=255)                                                        aGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGgcgc                                                                                >  2:231255/1‑140 (MQ=255)                                                         gCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGct                                                                               >  2:840805/1‑140 (MQ=255)                                                           cgtcgtGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGgcg                                                                                 <  2:244888/136‑1 (MQ=255)                                                           cgtcgtGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGgcg                                                                                 >  1:244888/1‑136 (MQ=255)                                                             tcgtGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTg                                                                           <  1:688407/140‑1 (MQ=255)                                                                  aaTGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCa                                                                      <  1:795843/140‑1 (MQ=255)                                                                    tGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCAtt                                                                    >  1:794888/1‑140 (MQ=255)                                                                         tAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGcc                                                               <  2:290608/140‑1 (MQ=255)                                                                            gCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAg                                                            >  1:1160305/1‑140 (MQ=255)                                                                             cccAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAGc                                                           <  2:719463/140‑1 (MQ=255)                                                                              ccAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAGCa                                                          <  1:876196/140‑1 (MQ=255)                                                                              ccAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAGCa                                                          <  1:380692/140‑1 (MQ=255)                                                                              ccAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAGCa                                                          <  2:1160305/140‑1 (MQ=255)                                                                              ccAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAGCa                                                          <  2:95805/140‑1 (MQ=255)                                                                                 gCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAGCAATg                                                       <  2:142098/140‑1 (MQ=255)                                                                                    gTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAGCAATGAGc                                                    <  1:1187090/140‑1 (MQ=255)                                                                                     tGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAGCAATGAGCt                                                   >  1:672988/1‑140 (MQ=255)                                                                                     tGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAGCAATGAGCt                                                   >  1:1274054/1‑140 (MQ=255)                                                                                      gAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAGCAATGAGc                                                    >  1:367244/1‑138 (MQ=255)                                                                                      gAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAGCAATGAGc                                                    <  2:367244/138‑1 (MQ=255)                                                                                         tGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAGCAATGAGCTATGt                                               <  1:840805/140‑1 (MQ=255)                                                                                          ggTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAGCAATGAGCTATGtt                                              <  2:879576/140‑1 (MQ=255)                                                                                           gTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCCCTTGCGGCATTTCGCCGAGCAATGAGCTATGttt                                             <  2:1208642/140‑1 (MQ=255)                                                                                            tAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAGCAATGAGCTATGTTTg                                            >  1:425097/1‑140 (MQ=255)                                                                                             aaCTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAGCAATGAGCTATGTTTGa                                           <  2:488507/140‑1 (MQ=255)                                                                                               cTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGGCCCAGGTTATACACCTGTGCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAGAAATGAGCTATGTTTGAAc                                         <  2:186586/140‑1 (MQ=255)                                                                                                 ttCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAGCAATGAGCTATGTTTGAACCa                                       >  1:433621/1‑140 (MQ=255)                                                                                                  tCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAGCAATGAGCTATGTTTGAACCAg                                      >  2:404857/1‑140 (MQ=255)                                                                                                     gCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAGCAATGAGCTATGTTTGAACCAGata                                   >  2:890986/1‑140 (MQ=255)                                                                                                          cgTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAGCAATGAGCTATGTTTGAACCAGa                                     >  2:440879/1‑133 (MQ=255)                                                                                                          cgTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAGCAATGAGCTATGTTTGAACCAGa                                     <  1:440879/133‑1 (MQ=255)                                                                                                          cgTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAGCAATGAGCTATGTTTGAACCAGATATcgcg                              >  2:811325/1‑140 (MQ=255)                                                                                                          cgTTTCCACGCTGATTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAGCAATGAGCTATGTTTGAACCAGATATcgcg                              >  1:580066/1‑140 (MQ=255)                                                                                                                       aTTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAGCAATGAGCTATGTTTGAACCAGATATCGCGGATATgcagca                   <  2:835724/138‑1 (MQ=255)                                                                                                                       aTTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAGCAATGAGCTATGTTTGAACCAGATATCGCGGATATgcagca                   >  1:835724/1‑138 (MQ=255)                                                                                                                       aTTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAGCAATGAGCTATGTTTGAACCAGATATCGCGGATATGCagcagc                 <  2:260497/140‑1 (MQ=255)                                                                                                                       aTTGCATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAGCAATGAGCTATGTTTGAACCAGATATCGCGGATATGCagcagc                 >  1:966716/1‑140 (MQ=255)                                                                                                                           cATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAGCAATGAGCTATGTTTGAACCAGATATCGCGGATATGCAGCAGCCAgt             <  2:966716/140‑1 (MQ=255)                                                                                                                           cATAATGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAGCAATGAGCTATGTTTGAACCAGATATCGCGGATATGCAGCAGCCAgt             <  1:404857/140‑1 (MQ=255)                                                                                                                              aaTGGTGGAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAGCAATGAGCTATGTTTGAACCAGATATCGCGGATATGCAGCAGCCAgttgt          <  2:395576/140‑1 (MQ=255)                                                                                                                                tggtggAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAGCAATGAGCTATGTTTGAACCAGATATCGCGGATATGCAGCAGCCAGttgttg        >  1:330778/1‑140 (MQ=255)                                                                                                                                 ggtggAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAGCAATGAGCTATGTTTGAACCAGATATCGCGGATATGCAGCAGCCAGTTGTTGa       >  1:735384/1‑140 (MQ=255)                                                                                                                                 ggtggAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAG‑CGGCGCTCTTGCGGCATTTCGCCGAGCAATGAGCTATGTTTGAACCAGATATCGCGGATATGCAGCAGCCAGTTGTTGAt      >  2:250371/1‑140 (MQ=255)                                                                                                                                  gtggAGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAGCAATGAGCTATGTTTGAACCAGATATCGCGGATATGCAGCAGCCAGTTGTTGAt      <  2:1116250/140‑1 (MQ=255)                                                                                                                                      aGTGACCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAGCAATGAGCTATGTTTGAACCAGATATCGCGGATATGCAGCAGCCAGTTGTTGATAAGc  >  1:1363554/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        TAACGTTGCTCAAGGGTTTCGCGGTTGGTGGCGGTAACATCCAGATCACGCAGCAAGCCGTCGTGAATGCCGTAGGCCCAGCCGTGAATGGTAACTTTCTGCCCGCGTTTCCACGCTGATTGCATAATGGTGGAGTGGCCCAGGTTATACACCTGTTCCATGACGTTCAGTTCACACAAGGTATCCAGACGGCGCTCTTGCGGCATTTCGCCGAGCAATGAGCTATGTTTGAACCAGATATCGCGGATATGCAGCAGCCAGTTGTTGATAAGC  >  NC_000913/142057‑142329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑