Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,335,943	T→C	L38P (CTA→CCA)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,335,943	0	T	C	100.0%	322.8 / NA	89	L38P (CTA→CCA)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (49/40);  total (49/40)

CCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTACCGCCC  >  NC_000913/1335810‑1336078                                                                                                                                      |                                                                                                                                        cccTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAACCACTGGGCGATATCACCCGTCCACCCaa                                                                                                                                   >  1:136821/1‑140 (MQ=255)  ccTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAg                                                                                                                                  <  1:1122544/140‑1 (MQ=255)   cTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGt                                                                                                                                 <  1:442224/140‑1 (MQ=255)    tGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTc                                                                                                                                >  1:512162/1‑140 (MQ=255)    tGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTc                                                                                                                                <  1:247825/140‑1 (MQ=255)         ttAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGTCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCa                                                                                                                           <  2:721071/140‑1 (MQ=255)             ggaggaGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGt                                                                                                                       >  1:110976/1‑140 (MQ=255)               aggagCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGttt                                                                                                                     <  1:963142/140‑1 (MQ=255)                ggagCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGtttt                                                                                                                    >  1:1015232/1‑140 (MQ=255)                ggagCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGtttt                                                                                                                    >  2:739022/1‑140 (MQ=255)                ggagCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGtttt                                                                                                                    >  2:668405/1‑140 (MQ=255)                 gagCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTg                                                                                                                   >  2:1265872/1‑140 (MQ=255)                  agCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGc                                                                                                                  >  1:1121708/1‑140 (MQ=255)                    cTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTc                                                                                                                >  1:1195706/1‑140 (MQ=255)                     tATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCg                                                                                                               <  1:668405/140‑1 (MQ=255)                      aTGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTTAAAGTTTTGCTCGa                                                                                                              >  1:436773/1‑140 (MQ=255)                      aTGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGa                                                                                                              >  2:392056/1‑140 (MQ=255)                        gtcgtcAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGaaa                                                                                                            >  2:272259/1‑140 (MQ=255)                        gtcgtcAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGaaa                                                                                                            >  2:274941/1‑140 (MQ=255)                                  cTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTgcg                                                                                                  <  1:979048/140‑1 (MQ=255)                                  cTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTgcg                                                                                                  <  2:512162/140‑1 (MQ=255)                                  cTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTgcg                                                                                                  <  1:168508/140‑1 (MQ=255)                                   tACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTgcgc                                                                                                 <  2:578325/140‑1 (MQ=255)                                       agaAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGc                                                                                             <  1:739022/140‑1 (MQ=255)                                        gaAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCa                                                                                            <  2:1015232/140‑1 (MQ=255)                                        gaAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCa                                                                                            <  2:1121708/140‑1 (MQ=255)                                         aaGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAg                                                                                           <  1:392056/140‑1 (MQ=255)                                          aGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACctgct                                                                                                     <  2:96863/129‑1 (MQ=255)                                          aGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACctgct                                                                                                     >  1:96863/1‑129 (MQ=255)                                           gCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCTGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAg                                                                                           <  1:925953/138‑1 (MQ=255)                                           gCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCTGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAg                                                                                           >  2:925953/1‑138 (MQ=255)                                               gTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGt                                                                                     <  1:141304/140‑1 (MQ=255)                                                tAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTa                                                                                    <  2:1074217/140‑1 (MQ=255)                                                  aGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAAc                                                                                  <  1:954809/140‑1 (MQ=255)                                                  aGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTCGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAAc                                                                                  <  2:1209492/140‑1 (MQ=255)                                                        cGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGt                                                                                     <  2:585000/131‑1 (MQ=255)                                                        cGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGt                                                                                     >  1:585000/1‑131 (MQ=255)                                                          ttGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTAc                                                                          <  1:1265872/140‑1 (MQ=255)                                                          ttGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTAc                                                                          >  2:633582/1‑140 (MQ=255)                                                            gCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCg                                                                        <  2:970282/140‑1 (MQ=255)                                                            gCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCg                                                                        <  1:274941/140‑1 (MQ=255)                                                             cAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGa                                                                       <  2:110976/140‑1 (MQ=255)                                                              aGGCCAGAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGaa                                                                      >  1:575147/1‑140 (MQ=255)                                                              aGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGaa                                                                      >  2:420853/1‑140 (MQ=255)                                                                  cAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAgg                                                                  >  1:1167252/1‑140 (MQ=255)                                                                  cAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCAAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAgg                                                                  >  2:1328362/1‑140 (MQ=255)                                                                  cAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCAAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAgg                                                                  <  1:1328362/140‑1 (MQ=255)                                                                     aGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAagag                                                                   <  2:982121/136‑1 (MQ=255)                                                                     aGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAagag                                                                   >  1:982121/1‑136 (MQ=255)                                                                         aaaaCTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCa                                                           <  1:420853/140‑1 (MQ=255)                                                                                aCCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTg                                                    >  2:183011/1‑140 (MQ=255)                                                                                 ccACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTgg                                                   >  2:1270602/1‑140 (MQ=255)                                                                                    ctactaCAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAg                                                >  2:142259/1‑140 (MQ=255)                                                                                     tactacAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAgg                                               >  2:1012184/1‑140 (MQ=255)                                                                                          cAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTAGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGc                                          >  1:316229/1‑140 (MQ=255)                                                                                           aGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCt                                         <  2:611681/140‑1 (MQ=255)                                                                                             ccTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGa                                       >  1:749399/1‑140 (MQ=255)                                                                                               tGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGaaa                                     <  1:183011/140‑1 (MQ=255)                                                                                               tGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGaaa                                     >  2:120028/1‑140 (MQ=255)                                                                                               tGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGaaa                                     >  1:955372/1‑140 (MQ=255)                                                                                               tGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGaaa                                     <  1:142259/140‑1 (MQ=255)                                                                                               tGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGaaa                                     >  1:828378/1‑140 (MQ=255)                                                                                               tGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGCTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGaaa                                     >  2:250651/1‑140 (MQ=255)                                                                                                 ccGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGaaaaa                                   <  1:704134/140‑1 (MQ=255)                                                                                                 ccGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGaaaaa                                   >  2:412274/1‑140 (MQ=255)                                                                                                 ccGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGaaaaa                                   >  1:620940/1‑140 (MQ=255)                                                                                                 ccGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGaaaaa                                   >  2:1112648/1‑140 (MQ=255)                                                                                                 ccGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGaaaaa                                   <  1:1112648/140‑1 (MQ=255)                                                                                                 ccGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGaaaaa                                   >  2:1033838/1‑140 (MQ=255)                                                                                                   gCTTGCTGCTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATg                                 <  1:990165/140‑1 (MQ=255)                                                                                                      tgctgcTAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGccc                              >  2:1213791/1‑140 (MQ=255)                                                                                                         tgctAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATg                           <  2:1195706/140‑1 (MQ=255)                                                                                                           ctAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCt                         <  2:1067129/140‑1 (MQ=255)                                                                                                            tAAATCACTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGCAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTg                        >  1:895549/1‑140 (MQ=255)                                                                                                                   cTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATg                                 >  2:480924/1‑124 (MQ=255)                                                                                                                   cTGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATg                                 <  1:480924/124‑1 (MQ=255)                                                                                                                    tGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGaa                >  2:566499/1‑140 (MQ=255)                                                                                                                    tGGGCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGaa                >  2:813511/1‑140 (MQ=255)                                                                                                                     gggCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATg                           >  1:233290/1‑128 (MQ=255)                                                                                                                     gggCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATg                           <  2:233290/128‑1 (MQ=255)                                                                                                                     gggCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGaaa               >  2:29627/1‑140 (MQ=255)                                                                                                                     gggCGATATCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGaaa               >  1:1130329/1‑140 (MQ=255)                                                                                                                          atatCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGcc          >  2:397672/1‑140 (MQ=255)                                                                                                                            atCACCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTa        >  1:641171/1‑140 (MQ=255)                                                                                                                               aCCCGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTACCg     <  1:1213791/140‑1 (MQ=255)                                                                                                                                cccGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTACCGc    >  1:1354593/1‑140 (MQ=255)                                                                                                                                cccGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTACCGc    >  1:1298390/1‑140 (MQ=255)                                                                                                                                cccGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTACCGc    >  2:1099577/1‑140 (MQ=255)                                                                                                                                  cGTCCACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTACCGccc  <  2:641171/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        CCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGTAACTCGGTTACCGAAGAGGATATCCACGCGCTGGCAGGATGGCTGAAAAATGCCCATGCTGACCGTGAAATTGCCTACCGCCC  >  NC_000913/1335810‑1336078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑