Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,537,145	C→T	W55* (TGG→TGA)	narW ←	NarW, putative private chaperone for NarZ nitrate reductase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,537,145	0	C	T	100.0%	327.4 / NA	91	W55* (TGG→TGA)	narW	NarW, putative private chaperone for NarZ nitrate reductase subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (41/50);  total (41/50)

AATCCAGCTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCATC  >  NC_000913/1537012‑1537280                                                                                                                                      |                                                                                                                                        aaTCCAGCTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGTAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCg                                                                                                                                   <  2:381235/140‑1 (MQ=255)  aTCCAGCTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCgg                                                                                                                                  <  2:257842/140‑1 (MQ=255)  aTCCAGCTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCgg                                                                                                                                  <  2:1047785/140‑1 (MQ=255)  aTCCAGCTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCgg                                                                                                                                  <  1:664685/140‑1 (MQ=255)   tCCAGCTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGc                                                                                                                                 <  2:1096337/140‑1 (MQ=255)   tCCAGCTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGc                                                                                                                                 >  2:561239/1‑140 (MQ=255)   tCCAGCTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGc                                                                                                                                 >  2:1046952/1‑140 (MQ=255)     cAGCTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACAATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCt                                                                                                                               <  2:1261730/140‑1 (MQ=255)       gCTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGt                                                                                                                             <  2:829167/140‑1 (MQ=255)          gCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTa                                                                                                                          <  2:978160/140‑1 (MQ=255)           cAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTAt                                                                                                                         >  1:589457/1‑140 (MQ=255)             ggCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATcc                                                                                                                       >  1:622204/1‑140 (MQ=255)                   ccTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCagcagc                                                                                                                 <  2:139893/140‑1 (MQ=255)                    cTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAgcg                                                                                                                <  1:1138659/140‑1 (MQ=255)                        tCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGgcgc                                                                                                            <  1:528170/140‑1 (MQ=255)                        tCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGgcgc                                                                                                            <  2:515550/140‑1 (MQ=255)                          aTACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGt                                                                                                          <  1:561239/140‑1 (MQ=255)                              tCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGt                                                                                                          >  2:626317/1‑136 (MQ=255)                              tCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGt                                                                                                          <  1:626317/136‑1 (MQ=255)                              tCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAg                                                                                                      <  1:782958/140‑1 (MQ=255)                              tCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAg                                                                                                      <  1:585269/140‑1 (MQ=255)                              cccGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAg                                                                                                      <  2:464271/139‑1 (MQ=255)                                cGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCa                                                                                                    >  2:1119164/1‑140 (MQ=255)                                 gCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAg                                                                                                   >  2:410889/1‑140 (MQ=255)                                  ccAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAg                                                                                                      >  1:174539/1‑136 (MQ=255)                                  ccAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAg                                                                                                      <  2:174539/136‑1 (MQ=255)                                  ccAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAgg                                                                                                  >  1:1130694/1‑140 (MQ=255)                                     gcagGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTtg                                                                                               <  1:488918/140‑1 (MQ=255)                                           ccaccaTTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTg                                                                                         <  1:46592/140‑1 (MQ=255)                                            caccaTTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGTGTGa                                                                                        <  2:548049/140‑1 (MQ=255)                                            caccaTTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGa                                                                                        <  1:1119164/140‑1 (MQ=255)                                                   gCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGt                                                                                 >  1:227799/1‑140 (MQ=255)                                                    ccTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGa                                                                                        >  1:302839/1‑132 (MQ=255)                                                    ccTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGa                                                                                        <  2:302839/132‑1 (MQ=255)                                                    ccTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTa                                                                                >  2:927096/1‑140 (MQ=255)                                                    ccTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTa                                                                                >  2:1364332/1‑140 (MQ=255)                                                    ccTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTa                                                                                <  2:113803/140‑1 (MQ=255)                                                         ccGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGaa                                                                                       <  1:371044/128‑1 (MQ=255)                                                         ccGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGaa                                                                                       >  2:371044/1‑128 (MQ=255)                                                         ccGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCAt                                                                           >  1:707173/1‑140 (MQ=255)                                                              aTCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGgcg                                                                      >  1:292897/1‑140 (MQ=255)                                                              aTCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGgcg                                                                      >  1:890876/1‑140 (MQ=255)                                                              aTCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGgcg                                                                      <  1:1096978/140‑1 (MQ=255)                                                                  cgGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTc                                                                  <  2:227799/140‑1 (MQ=255)                                                                    ggACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTcgc                                                                >  2:495678/1‑140 (MQ=255)                                                                      aCTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGTGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCgcgg                                                              <  1:123712/140‑1 (MQ=255)                                                                      aCTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCgcgg                                                              <  1:150992/140‑1 (MQ=255)                                                                         cGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGcggcgg                                                           >  1:958265/1‑140 (MQ=255)                                                                          ggCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGa                                                          >  1:889160/1‑140 (MQ=255)                                                                          ggCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGa                                                          >  2:73204/1‑140 (MQ=255)                                                                          ggCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGa                                                          >  2:1368084/1‑140 (MQ=255)                                                                              tGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCaa                                                      <  1:1046952/140‑1 (MQ=255)                                                                                  cATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGcc                                                  <  2:1336528/140‑1 (MQ=255)                                                                                  cATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGcc                                                  <  2:292897/140‑1 (MQ=255)                                                                                     gTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAgc                                               >  1:660007/1‑140 (MQ=255)                                                                                      ttCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAgcg                                              >  2:458798/1‑140 (MQ=255)                                                                                      ttCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAgcg                                              >  1:780040/1‑140 (MQ=255)                                                                                        cGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGcgcg                                            <  1:458798/140‑1 (MQ=255)                                                                                        cGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGcgcg                                            <  1:1364332/140‑1 (MQ=255)                                                                                        cGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGcgcg                                            <  2:622204/140‑1 (MQ=255)                                                                                        cGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGcgcg                                            <  1:1006268/140‑1 (MQ=255)                                                                                         gAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGt                                           <  2:779520/140‑1 (MQ=255)                                                                                         gAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGt                                           <  2:19311/140‑1 (MQ=255)                                                                                         gAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGt                                           <  2:1196929/140‑1 (MQ=255)                                                                                         gAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGCCGCGGCGGATCAACGCCAGCGCGt                                           <  2:589457/140‑1 (MQ=255)                                                                                          aaCAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGtc                                          >  2:1047052/1‑140 (MQ=255)                                                                                          aaCAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGtc                                          >  1:1119980/1‑140 (MQ=255)                                                                                          aaCAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGtc                                          >  1:1082223/1‑140 (MQ=255)                                                                                           aCAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGtcc                                         <  2:1082223/140‑1 (MQ=255)                                                                                            cagcagcagCGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGtcct                                        <  2:958265/140‑1 (MQ=255)                                                                                                  cagcGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGc                                  <  2:306192/140‑1 (MQ=255)                                                                                                  cagcGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGc                                  >  2:1359677/1‑140 (MQ=255)                                                                                                     cGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAgcgc                                             >  1:1077568/1‑126 (MQ=255)                                                                                                     cGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAgcgc                                             <  2:1077568/126‑1 (MQ=255)                                                                                                     cGACGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCAtt                               <  2:1119980/140‑1 (MQ=255)                                                                                                       aCGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTcc                             >  1:29177/1‑140 (MQ=255)                                                                                                       aCGTGGTGCGCCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTcc                             <  2:780040/140‑1 (MQ=255)                                                                                                               cgcCCGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAg                     <  1:1368084/140‑1 (MQ=255)                                                                                                                 cccGCGGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCt                   <  2:889160/140‑1 (MQ=255)                                                                                                                   cgcgGTCAAACACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCg                 >  2:611806/1‑140 (MQ=255)                                                                                                                          aaaCACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGAt          <  1:410889/140‑1 (MQ=255)                                                                                                                          aaaCACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGAt          <  1:1359677/140‑1 (MQ=255)                                                                                                                          aaaCACTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGAt          <  2:1130694/140‑1 (MQ=255)                                                                                                                               cTTCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTcc     >  1:262837/1‑140 (MQ=255)                                                                                                                                ttCGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGGTACTCCa    >  2:978513/1‑140 (MQ=255)                                                                                                                                  cGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCATc  >  1:1119725/1‑140 (MQ=255)                                                                                                                                  cGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCATc  >  1:381436/1‑140 (MQ=255)                                                                                                                                  cGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCATc  >  2:371103/1‑140 (MQ=255)                                                                                                                                  cGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCATc  >  1:492087/1‑140 (MQ=255)                                                                                                                                  cGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCATc  >  2:263311/1‑140 (MQ=255)                                                                                                                                  cGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCATc  >  1:520700/1‑140 (MQ=255)                                                                                                                                  cGCATCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCATc  >  1:983040/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                        AATCCAGCTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGCGACGTGGTGCGCCCGCGGTCAAACACTTCGCACCATTCGGCCTGTTTATCCAGCAGCGGCGCGTTAAGCAGGTTGTGGGTGAAATCCGTAAGCATCGGCGCGTCGCGGCGGATCAACGCCAGCGCGTCCTCCTTGCATTCCCACAACAGCTCGTCCGGATACTCCATC  >  NC_000913/1537012‑1537280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑