Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,537,724	A→G	L377P (CTG→CCG)	narY ←	nitrate reductase Z subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,537,724	0	A	G	100.0%	289.4 / NA	80	L377P (CTG→CCG)	narY	nitrate reductase Z subunit beta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/45);  total (35/45)

TTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCAGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTACCAGACCATCGGCAGGGTGCGGTATTCAGGGTGCAACGGTAGTGCCAGTTTCCAGTCCATCGCCA  >  NC_000913/1537588‑1537859                                                                                                                                         |                                                                                                                                        ttCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGgcggca                                                                                                                                      <  1:470182/140‑1 (MQ=255) ttCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGgcggca                                                                                                                                      <  2:61475/140‑1 (MQ=255) ttCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGgcggca                                                                                                                                      <  1:483699/140‑1 (MQ=255) ttCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGgcggca                                                                                                                                      <  2:442002/140‑1 (MQ=255) ttCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGgcggca                                                                                                                                      <  1:540454/140‑1 (MQ=255)  tCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCAc                                                                                                                                     >  2:520779/1‑140 (MQ=255)  tCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCAc                                                                                                                                     <  2:784792/140‑1 (MQ=255)  tCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCAc                                                                                                                                     >  2:756941/1‑140 (MQ=255)    cACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGc                                                                                                                                   >  1:748839/1‑140 (MQ=255)           tgtgAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCt                                                                                                                            >  1:164844/1‑140 (MQ=255)           tgtgAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCt                                                                                                                            <  2:781865/140‑1 (MQ=255)           tgtgAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCt                                                                                                                            <  2:1272138/140‑1 (MQ=255)              gAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCtttt                                                                                                                         >  2:628727/1‑140 (MQ=255)                   cATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCa                                                                                                                    >  1:534332/1‑140 (MQ=255)                   cATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCa                                                                                                                    >  2:266249/1‑140 (MQ=255)                   cATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCa                                                                                                                    >  1:847226/1‑140 (MQ=255)                          tGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAAccgcc                                                                                                             <  1:553070/140‑1 (MQ=255)                          tGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAAccgcc                                                                                                             <  2:847226/140‑1 (MQ=255)                            gcgcATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAAccgcccg                                                                                                           >  2:1202561/1‑140 (MQ=255)                            gcgcATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAAccgcccg                                                                                                           >  1:331192/1‑140 (MQ=255)                            gcgcATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAAccgcccg                                                                                                           >  2:610422/1‑140 (MQ=255)                            gcgcATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAAccgcccg                                                                                                           >  2:628714/1‑140 (MQ=255)                            gcgcATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAAccgcc                                                                                                             <  1:464651/138‑1 (MQ=255)                            gcgcATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAAccgcc                                                                                                             >  2:464651/1‑138 (MQ=255)                             cgcATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAAGATATTGGCGAGATATTGCACCGGAATACACAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAAccgcccgc                                                                                                          >  2:942995/1‑140 (MQ=255)                              gcATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAAccgcc                                                                                                             <  1:268694/136‑1 (MQ=255)                              gcATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAAccgcc                                                                                                             >  2:268694/1‑136 (MQ=255)                               cATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCAt                                                                                                        >  1:827058/1‑140 (MQ=255)                               cATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCAt                                                                                                        <  1:756941/140‑1 (MQ=255)                                  cGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTg                                                                                                     >  2:187249/1‑140 (MQ=255)                                    ccATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCg                                                                                                   <  2:748839/140‑1 (MQ=255)                                    ccATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCg                                                                                                   <  1:819090/140‑1 (MQ=255)                                    ccATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCg                                                                                                   <  2:47053/140‑1 (MQ=255)                                    ccATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCg                                                                                                   <  1:1202561/140‑1 (MQ=255)                                    ccATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCg                                                                                                   <  1:1250783/140‑1 (MQ=255)                                    ccATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCg                                                                                                   <  1:628714/140‑1 (MQ=255)                                         atcCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGa                                                                                              <  1:266249/140‑1 (MQ=255)                                               ttCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAAt                                                                                        >  1:666715/1‑140 (MQ=255)                                               ttCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAAt                                                                                        >  2:698794/1‑140 (MQ=255)                                                   gcgcCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCg                                                                                                   <  1:393493/125‑1 (MQ=255)                                                   gcgcCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCg                                                                                                   >  2:393493/1‑125 (MQ=255)                                                     gcCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGa                                                                                  <  2:374582/140‑1 (MQ=255)                                                        cGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAg                                                                               >  2:572927/1‑140 (MQ=255)                                                            gTACCGGACCGGTATCGCCGGCACT‑AACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCggag                                                                          <  2:475559/140‑1 (MQ=255)                                                               ccggaccggTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCggagga                                                                        <  2:873087/140‑1 (MQ=255)                                                                cggaccggTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGaa                                                                       <  1:610422/140‑1 (MQ=255)                                                                cggaccggTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGaa                                                                       <  1:41154/140‑1 (MQ=255)                                                                cggaccggTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGaa                                                                       <  1:898337/140‑1 (MQ=255)                                                                      ggTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTAcc                                                                 <  1:187249/140‑1 (MQ=255)                                                                      ggTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTAcc                                                                 <  1:266760/140‑1 (MQ=255)                                                                       gTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGAc                                                                                 <  1:155279/123‑1 (MQ=255)                                                                       gTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGAc                                                                                 >  2:155279/1‑123 (MQ=255)                                                                       gTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTACCa                                                                >  2:375611/1‑140 (MQ=255)                                                                             ccGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTACCAGACCAt                                                          >  2:307867/1‑140 (MQ=255)                                                                                gCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTACCAGACCATCgg                                                       <  1:572927/140‑1 (MQ=255)                                                                                 cACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTACCAGACCATCGGc                                                      <  2:827058/140‑1 (MQ=255)                                                                                  aCTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTACCAGACCATCGGCa                                                     >  1:587270/1‑140 (MQ=255)                                                                                  aCTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTACCAGACCATCGGCa                                                     <  1:375611/140‑1 (MQ=255)                                                                                    tCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTACCAGACCATCGGCAgg                                                   <  1:698794/140‑1 (MQ=255)                                                                                    tCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTACCAGACCATCGGCAgg                                                   <  1:820669/140‑1 (MQ=255)                                                                                      aaCATATTGGCGAGATATTGCACCGGAATACGCAGGCGTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAGTCGGTGATAGCGGAGGAACGTACCAGACCATCGGGAgggg                                                 >  2:1206871/1‑139 (MQ=255)                                                                                       aCATATTGGCGAGATATTGCACCGGAATACGGAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTACCAGACCATCGGCAGGGTg                                                <  1:91585/140‑1 (MQ=255)                                                                                         atatTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTACCAGACCATCGGCAGGGTGCg                                              <  1:1210004/140‑1 (MQ=255)                                                                                            ttGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTACCAGACCATCGGCAGGGTGCGGTa                                           >  2:386344/1‑140 (MQ=255)                                                                                              ggCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGG‑GGAACGTACCAGACCATCGGCAGGGTg                                                >  2:1043310/1‑132 (MQ=255)                                                                                              ggCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGG‑GGAACGTACCAGACCATCGGCAGGGTg                                                <  1:1043310/132‑1 (MQ=255)                                                                                                cGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTACCAGACCATCGGCAGGGGGCGGTATTCa                                       >  1:993788/1‑140 (MQ=255)                                                                                                  agaTATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTACCAGACCATCGGCAGGGTGCGGTATTCAgg                                     >  2:912481/1‑140 (MQ=255)                                                                                                  agaTATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTACCAGACCATCGGCAGGGTGCGGTATTCAgg                                     >  1:1136971/1‑140 (MQ=255)                                                                                                   gaTATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTACCAGACCATCGGCAGGGTGCGGTATTCAggg                                    <  2:335514/140‑1 (MQ=255)                                                                                                   gaTATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTACCAGACCATCGGCAGGGTGCGGTATTCAggg                                    <  2:964445/140‑1 (MQ=255)                                                                                                         gCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTACCAGACCATCGGCAGGGTGCGGTATTCAGGGTGCAAc                              <  1:1206871/140‑1 (MQ=255)                                                                                                         gCACCGGAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTACCAGACCATCGGCAGGGTGCGGTATTCAGGGTGCAAc                              >  1:644899/1‑140 (MQ=255)                                                                                                              ggAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTACCAGACCATCGGCAGGGTGCGGTATTCAGGGTGCAACGGTAg                         <  1:1086748/140‑1 (MQ=255)                                                                                                              ggAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTACCAGACCATCGGCAGGGTGCGGTATTCAGGGTGCAACGGTAg                         >  2:41184/1‑140 (MQ=255)                                                                                                               gAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTACCAGACCATCGGCAGGGTGCGGTATTCAGGGTGCAACGGTAGt                        >  2:1164570/1‑140 (MQ=255)                                                                                                               gAATACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTACCAGACCATCGGCAGGGTGCGGTATTCAGGGTGCAACGGTAGt                        >  2:927055/1‑140 (MQ=255)                                                                                                                aaTACGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTACCAGACCATCGGCAGGGTGCGGTATTCAGGGTGCAACGGTAGTg                       <  1:520779/140‑1 (MQ=255)                                                                                                                   aCGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTACCAGACCATCGGCAGGGTGCGGTATTCAGGGTGCAACGGTAGTGCCa                    <  1:927055/140‑1 (MQ=255)                                                                                                                   aCGCAGGCTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTACCAGACCATCGGCAGGGTGCGGTATTCAGGGTGCAACGGTAGTGCCa                    <  1:628727/140‑1 (MQ=255)                                                                                                                          cTTTCGATGGCGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTACCAGACCATCGGCAGGGTGCGGTATTCAGGGGGCAACGGTAGTGCCAGTTTCCa             >  1:230483/1‑140 (MQ=255)                                                                                                                                   gcgggcggCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTACCAGACCATCGGCAGGGTGCGGTATTCAGGGTGCAACGGTAGTGCCAGTTTCCAGTCCATCGc    >  2:1090031/1‑140 (MQ=255)                                                                                                                                   gcgggcggCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTACCAGACCATCGGCAGGGTGCGGTATTCAGGGTGCAACGGTAGTGCCAGTTTCCAGTCCATAGc    >  2:1181006/1‑140 (MQ=255)                                                                                                                                    cGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTACCAGACCATCGGCAGGGTGCGGTATTCAGGGTGCAACGGTAGTGCCAGTTTCCAGTCCATCGcc   <  2:644899/140‑1 (MQ=255)                                                                                                                                    cGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTACCAGACCATCGGCAGGGTGCGGTATTCAGGGTGCAACGGTAGTGCCAGTTTCCAGTCCATCGcc   <  1:912268/140‑1 (MQ=255)                                                                                                                                    cGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTACCAGACCATCGGCAGGGTGCGGTATTCAGGGTGCAACGGTAGTGCCAGTTTCCAGTCCATCGcc   <  1:689983/140‑1 (MQ=255)                                                                                                                                    cGGGCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTACCAGACCATCGGCAGGGTGCGGTATTCAGGGTGCAACGGTAGTGCCAGTTTCCAGTCCATCGcc   <  1:513224/140‑1 (MQ=255)                                                                                                                                     gggCGGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTACCAGACCATCGGCAGGGTGCGGTATTCAGGGTGCAACGGTAGTGCCAGTTTCCAGTCCATCGCCa  <  2:331192/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        TTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGAGATATTGCACCGGAATACGCAGGCTTTCGATGGCGGGCAGCACGCCTTCGCTTTTCGGCAAACCGCCCGCATCTGCGTAGGACTGAATCGGTGACAGCGGAGGAACGTACCAGACCATCGGCAGGGTGCGGTATTCAGGGTGCAACGGTAGTGCCAGTTTCCAGTCCATCGCCA  >  NC_000913/1537588‑1537859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑