Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	179,819	A→G	T195A (ACA→GCA)	dgt →	dGTP triphosphohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	179,819	0	A	G	100.0%	200.8 / NA	56	T195A (ACA→GCA)	dgt	dGTP triphosphohydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/30);  total (26/30)

TCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATACATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAA  >  NC_000913/179683‑179953                                                                                                                                         |                                                                                                                                       tctGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTgcatgcat                                                                                                                                     <  2:507489/140‑1 (MQ=255)          cGATCGCTGCAGCGTGGCGGCACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGa                                                                                                                            >  2:151659/1‑140 (MQ=255)           gATCGCTGCAGCGTGGCGGCACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGAt                                                                                                                           <  2:51255/140‑1 (MQ=255)                cTGCAGCGTGGCGGCACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAAtc                                                                                                                      >  2:1170877/1‑140 (MQ=255)                   cAGCGTGGCGGCACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCa                                                                                                                   >  1:1355228/1‑140 (MQ=255)                    aGCGTGGCGGCACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCAc                                                                                                                  <  1:1170877/140‑1 (MQ=255)                     gCGTGGCGGCACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCAcc                                                                                                                 <  1:1214175/140‑1 (MQ=255)                      cGTGGCGGCACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCt                                                                                                                <  1:151659/140‑1 (MQ=255)                        tGGCGGCACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTgg                                                                                                              >  2:230734/1‑140 (MQ=255)                        tGGCGGCACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTgg                                                                                                              >  2:557276/1‑140 (MQ=255)                        tGGCGGCACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTgg                                                                                                              >  2:970034/1‑140 (MQ=255)                         ggcggcACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTggg                                                                                                             >  2:233112/1‑140 (MQ=255)                           cggcACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGca                                                                                                           >  1:828684/1‑140 (MQ=255)                           cggcACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGca                                                                                                           >  2:156385/1‑140 (MQ=255)                           cggcACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGca                                                                                                           <  2:1213826/140‑1 (MQ=255)                           cggcACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGca                                                                                                           <  1:391596/140‑1 (MQ=255)                           cggcACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGca                                                                                                           >  2:709308/1‑140 (MQ=255)                           cggcACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGca                                                                                                           <  2:1203295/140‑1 (MQ=255)                                   cGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTgg                                                                                                   <  1:557276/140‑1 (MQ=255)                                     tttACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCg                                                                                                 <  1:839094/140‑1 (MQ=255)                                     tttACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCg                                                                                                 <  1:1155471/140‑1 (MQ=255)                                           ggacgggGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTAttt                                                                                           <  2:1355228/140‑1 (MQ=255)                                              cgggGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTaa                                                                                        >  2:392391/1‑140 (MQ=255)                                               ggggAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTaaa                                                                                       >  2:833846/1‑140 (MQ=255)                                                 ggAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAat                                                                                     >  2:448135/1‑140 (MQ=255)                                                  gaagaaCCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAata                                                                                    <  1:780973/140‑1 (MQ=255)                                                   aagaaCCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAatat                                                                                   >  2:106993/1‑140 (MQ=255)                                                     gaaCCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATAc                                                                                 <  1:412741/140‑1 (MQ=255)                                                          gCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTc                                                                            <  1:106993/140‑1 (MQ=255)                                                           cTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTcc                                                                           >  1:536276/1‑140 (MQ=255)                                                           cTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTcc                                                                           >  2:747533/1‑140 (MQ=255)                                                                cGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCgt                                                                      >  1:551991/1‑140 (MQ=255)                                                                            cAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGtggcgtggcg                                                          >  2:970509/1‑140 (MQ=255)                                                                              aGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGaa                                                        <  1:374814/140‑1 (MQ=255)                                                                                    gTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCG‑GGCGAAACGCCTg                                                 >  2:517156/1‑140 (MQ=255)                                                                                        ggACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTgaga                                              <  1:233112/140‑1 (MQ=255)                                                                                        ggACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTgaga                                              <  2:536276/140‑1 (MQ=255)                                                                                        ggACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTgaga                                              <  2:1301727/140‑1 (MQ=255)                                                                                          aCTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGaca                                            <  1:448135/140‑1 (MQ=255)                                                                                          aCTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGaca                                            <  1:1026713/140‑1 (MQ=255)                                                                                          aCTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGaca                                            <  1:970509/140‑1 (MQ=255)                                                                                          aCTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGaca                                            <  1:970034/140‑1 (MQ=255)                                                                                             tATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGacacgt                                         <  1:230734/140‑3 (MQ=255)                                                                                               tGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACACGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCa                                       <  2:1201494/140‑1 (MQ=255)                                                                                                   aTTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGaca                                            <  1:1368197/131‑1 (MQ=255)                                                                                                   aTTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGaca                                            >  2:1368197/1‑131 (MQ=255)                                                                                                    ttttGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGaca                                            >  1:765346/1‑130 (MQ=255)                                                                                                    ttttGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGaca                                            <  2:765346/130‑1 (MQ=255)                                                                                                         aGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATg                             >  1:719928/1‑140 (MQ=255)                                                                                                                tGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGc                      >  1:318188/1‑140 (MQ=255)                                                                                                                tGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGc                      <  1:747533/140‑1 (MQ=255)                                                                                                                 gCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGcc                     <  2:318188/140‑1 (MQ=255)                                                                                                                     aaGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGt                 >  2:51464/1‑140 (MQ=255)                                                                                                                          aTTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGttatta            <  1:95116/140‑1 (MQ=255)                                                                                                                           ttCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGttatta            <  2:742441/139‑1 (MQ=255)                                                                                                                           ttCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGttatta            >  1:742441/1‑139 (MQ=255)                                                                                                                            tCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATc          >  2:1251122/1‑140 (MQ=255)                                                                                                                                    tgcatgcaTTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTgaa  <  1:1251122/140‑1 (MQ=255)                                                                                                                                    tgcatgcaTTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTgaa  <  1:1093913/140‑1 (MQ=255)                                                                                                                                    tgcatgcaTTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTgaa  <  1:833846/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       TCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATACATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAA  >  NC_000913/179683‑179953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑